Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pT2/CMV-PDGFA-IRES-DsRed (#RDB16061)

The Sleeping Beauty (SB) transposon system clone. Expression vector of human PDGFA.

Alternative name P vector
Clone info. The Sleeping Beauty (SB) transposon system clone. Expression vector of human platelet-derived growth factor subunit A (PDGFA). DsRed is expressed as a marker. Please refer to Fig.1A of Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pT2/Onc2 (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r
Gene/insert name Human PDGFA cDNA
Depositor|Developer Koso, Hideto |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
In order to receive this biological resource, please request the Exclusive MTA to us.
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018).
Remarks This clone is provided directly from Dr. Hideto Koso of the Institute of Medical Science, Tokyo University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sumiyoshi, K. et al. Cancer Sci., 109 (5): 1513-1523, 2018)。
備考 本クローンは、東京大学医科学研究所の高祖秀登先生から直接提供いたします。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16061 pT2/CMV-PDGFA-IRES-DsRed DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pT2/CMV-PDGFA-IRES-DsRed was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16061).

Reference section:

Sumiyoshi, K., Koso, H., Watanabe, S., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16061_A8Idp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB16061_A8Ida.seq check
>D05854A2-3_A8Id_CMV-Forward_A01_01_ABI24.ab1
    1 CTTTACCACT TCCGCCCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GACTCAGATC
  121 TCGAGCTCAA GCTTCGAATT CACTAGTGAT TGCGATGAGG ACCTTGGCTT GCCTGCTGCT
  181 CCTCGGCTGC GGATACCTCG CCCATGTTCT GGCCGAGGAA GCCGAGATCC CCCGCGAGGT
  241 GATCGAGAGG CTGGCCCGCA GTCAGATCCA CAGCATCCGG GACCTCCAGC GACTCCTGGA
  301 GATAGACTCC GTAGGGAGTG AGGATTCTTT GGACACCAGC CTGAGAGCTC ACGGGGTCCA
  361 CGCCACTAAG CATGTGCCCG AGAAGCGGCC CCTGCCCATT CGGAGGAAGA GAAGCATCGA
  421 GGAAGCTGTC CCCGCTGTCT GCAAGACCAG GACGGTCATT TACGAGATTC CTCGGAGTCA
  481 GGTCGACCCC ACGTCCGCCA ACTTCCTGAT CTGGCCCCCG TGCGTGGAGG TGAAACGCTG
  541 CACCGGCTGC TGCAACACGA GCAGTGTCAA GTGCCAGCCC TCCCGCGTCC ACCACCGCAG
  601 CGTCAAGGTG GCCAAGGTGG AATACGTCAG GAAGAAGCCA AAATTAAAAG AAGTCCAGGT
  661 GAGGTTAGAG GAGCATTTGG AGTGCGCCTG CGCGACCACA AGCCTGAATC CGGATTATCG
  721 GGAAGAGGAC ACGGGAAGGC CTAGGGAGTC AGGTAAAAAA CGGAAAAGAA AAAGGTTAAA
  781 ACCCACCTAA AGCAGCCAAC CAGATAATCG AATTCTGCAG TCGACGGTAC CGCGGGCCCG
  841 GGATCCGCCC CTCTCCCTCC CCCCCCCCCC CTAACGTTAC TGGGCCGAAG CCCGCTTGGA
  901 AATAAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTTGG
  961 CAATGTGAGG GCCCGGGAAC CTTGACCCTG TCTTCTTGAA CGAGCATCTT AGGGTCCTTT
1021 CCCCTCTCGC AAGGATGCAG GTCTGATTGT ATGTCCGTGA AGGAAGCAAG
//
Primer: Red-N_R
Sequence file: RDB16061_A8Idb.seq check
>D05854A2-3_A8Id_Red-N_R_B01_04_ABI24.ab1
    1 GTTATAATCG GCAACTTGAA GCGCATGAAC TCCTTGATGA CGTCCCTCGG AGGAGGCCAT
   61 GGTTGTGGCC ATATTATCAT CGTGTTTTTC AAAGGAAAAC CACGTCCCCG TGGTTCGGGG
  121 GGCCTAGACG TTTTTTTAAC CTCGACTAAA CACATGTAAA GCATGTGTAC CGAGGCCCCA
  181 GATCAGATCC CATACAATGG GGTACCTTCT GGGCATCCTT CAGCCCCTTG TTGAATACGC
  241 TTGAGGAGAG CCATTTGACT CTTTCCACAA CTATCCAACT CACAACGTGG CACTGGGGTT
  301 GTGCCGCCTT TGCAGGTGTA TCTTATACAC GTGGCTTTTG GCCGCAGAGG CACCTGTCGC
  361 CAGGTGGGGG GTTCCGCTGC CTGCAAAGGG TCGCTACAGA CGTTGTTTGT CTTCAAGAAG
  421 CTTCCAGAGG AACTGCTTCC TTCACGACAT TCAACAGACC TTGCATTCCT TTGGCGAGAG
  481 GGGAAAGACC CCTAGGAATG CTCGTCAAGA AGACAGGGCC AGGTTTCCGG GCCCTCACAT
  541 TGCCAAAAGA CGGCAATATG GTGGAAAATA ACATATAGAC AAACGCACAC CGGCCTTATT
  601 CCAAGCGGCT TCGGCCAGTA ACGTTAGGGG GGGGGGAGGG AGAGGGGCGG ATCCCGGGCC
  661 CGCGGTACCG TCGACTGCAG AATTCGATTA TCTGGTTGGC TGCTTTAGGT GGGTTTTAAC
  721 CTTTTTCTTT TCCGTTTTTT ACCTGACTCC CTAGGCCTTC CCGTGTCCTC TTCCCGATAA
  781 TCCGGATTCA GGCTTGTGGT CGCGCAGGCG CACTCCAAAT GCTCCTCTAA CCTCACCTGG
  841 ACTTCTTTTA ATTTTGGCTT CTTCCTGACG TATTCCACCT TGGCCACCTT GACGCTGCGG
  901 TGGTGGACGC GGGAGGGCTG GCACTTGACA CTGCTCGTGT TGCAGCAGCC GGGTGCAGCG
  961 TTTCCACCCT CCACGCACGG GGGCCCAGAT CAGGGAAAGT TGGGCGGACG TGGGGTCGAC
 1021 CTTGAACTTC CGAAGATCTC GTAAATGGAC GTCCCTGGTC TTGCAGAACA GCCGGGACAG
1081 GCTACTCGAT GCATTTCATC CGTA
//
Primer: M13_-40
Sequence file: RDB16061_A8Idc.seq check
>D05854A2-3_A8Id_M13_-40_C01_07_ABI24.ab1
    1 CACAGTGACG CGCGTATACG ACTCACTATA GGGCGAATTG GAGCTCGGAT CCCTATACAG
   61 TTGAAGTCGG AAGTTTACAT ACACTTAAGT TGGAGTCATT AAAACTCGTT TTTCAACTAC
  121 TCCACAAATT TCTTGTTAAC AAACAATAGT TTTGGCAAGT CAGTTAGGAC ATCTACTTTG
  181 TGCATGACAC AAGTCATTTT TCCAACAATT GTTTACAGAC AGATTATTTC ACTTATAATT
  241 CACTGTATCA CAATTCCAGT GGGTCAGAAG TTTACATACA CTAAGTTGAC TGTGCCTTTA
  301 AACAGCTTGG AAAATTCCAG AAAATGATGT CATGGCTTTA GAAGCTATCT AGTTATTAAT
  361 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
  421 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA
  481 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT
  541 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC
  601 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT
  661 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC
  721 GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC
  781 TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA
  841 AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG
  901 GTCTATATAA GCAGAGCTGG TTTAGTGAAA CCGTCAGATC CGCTAGCGCT ACCGGGACTC
  961 AGATCTCGAG CTCAAGCTTC GAAATTTCAC TAGTGATTGC GATGAAGGAC CTTTGGGCTT
 1021 TGCCCTGCTG CTCGTCGACT GGCCGGATAC GATCCGCCCA TGTCTGCGAA GGAAGCCGAG
1081 ATCCCCCCGC CGAG
//
Primer: reverse2
Sequence file: RDB16061_A8Idd.seq check
>D05854A2-3_A8Id_reverse2_D01_10_ABI24.ab1
    1 AGTAAGGCAT TCGCCAAGCG CGCATTAACC CTCACTAAAG GGAACAAAAG CTGGGTACCG
   61 GGCCCCCCCT CGAGGTCGAC TCTAGCTAGA GGATCCCTAT ACAGTTGAAG TCGGAAGTTT
  121 ACATACACCT TAGCCAAATA CATTTAAACT CACTTTTTCA CAATTCCTGA CATTTAATCC
  181 TAGTAAAAAT TCCCTGTCTT AGGTCAGTTA GGATCACCAC TTTATTTTAA GAATGTGAAA
  241 TATCAGAATA ATAGTAGAGA GAATGATTCA TTTCAGCTTT TATTTCTTTC ATCACATTCC
  301 CAGTGGGTCA GAAGTTTACA TACACTCAAT TAGTATTTGG TAGCATTGCC TTTAAATTGT
  361 TTAACTTGGG TCAAACATTT CGAGTAGCCT TCCACAAGCT AGATCCCCTA GTTTGTGATA
  421 GGAATTAGCT TTTCCGCAAG CTCTAGTCGA GCCCCAGCTG GTTCTTTCCG CCTCAGAAGC
  481 CATAGAGCCC ACCGCATCCC CAGCATGCCT GCTATTGTCT TCCCAATCCT CCCCCTTGCT
  541 GTCCTGCCCC ACCCCACCCC CCAGAATAGA ATGACACCTA CTCAGACAAT GCGATGCAAT
  601 TTCCTCATTT TATTAGGAAA GGACAGTGGG AGTGGCACCT TCCAGGGTCA AGGAAGGCAC
  661 GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC ACAGTCGAGG CTGATCAGCG
  721 AGCTCTAGAG AATTGATCGC GGCCGCTACA GGAACAGGTG GTGGCGGCCC TCGGCGCGCT
  781 CGTACTGCTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAGC TTGGAGTCCA
  841 CGTAGTAGTA GCCGGGCAGC TGCACGGGCT TCTTGGCCAT ATAGATAGAC TTGAACTCCA
  901 CCAGGTAGTG GCCGCCGTCC TTCAGCTTCA GGGCCCTTGT GGATCTCGCC CTTCAGCACG
  961 CCGTCGCGGG GGGTACAGCG CTCGGGTGGG AGGCCCTCCC AGCCCATAGT TCTTCGTCTG
 1021 CATTTACGGG GCCGGTCGAA GGGGAGGTTC ACGTCGATGG AACCTCACCT TGCTAGGATG
1081 GAAGGGAGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sumiyoshi, K., Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma. Cancer Sci. 109 (5): 1513-1523 (2018). PMID 29575648. [link to RRC of NBRP]