RDB16059 - pCAG-Arl13bCerulean-Fucci2a

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pCAG-Arl13bCerulean-Fucci2a (#RDB16059)

Mammalian expression construct of Arl13bCerulean-Fucci2a

Clone info.	Mammalian expression construct containng the CAG promoter drving Arl13bCerulean-Fucci2a a multicistronic version of Fucci2 composed of Arl13bCerulean, mVenus-hGem(1/110) and mCherry-hCdt1(30/120) fused with t2a and p2a.
Comment	This clone was used in subcloning pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a by the depositor.
Vector backbone	pCAGiP (Plasmid)
Selectable markers	Ampicillin
Gene/insert name	Human CDT1 cDNA Human Gem cDNA Mouse Arl13b cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Jackson, Ian \| Mort, Richard \|

Sequence (full)	RDB16059hts01.seq Assembled from experimentally sequenced data.
Publication	Ford, M.J., Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140. [link to RRC of NBRP]
Test sheet	RDB16059_B1E6p1-2.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested.
3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
Additional terms and conditions:
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki.

Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. 6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties. Additional terms and conditions: 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki.
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. 6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties. 付加的使用条件： 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki.
備考	提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16059	pCAG-Arl13bCerulean-Fucci2a	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCAG-Arl13bCerulean-Fucci2a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16059).

Reference section:

Ford, M.J., Yeyati, P.L., Mali, G.R., Keighren, M.A., Waddell, S.H., Mjoseng, H.K., Douglas, A.T., Hall, E.A., Sakaue-Sawano, A., Miyawaki, A., Meehan, R.R., Boulter, L., Jackson, I.J., Mill, P., Mort, R.L., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140. [link to RRC of NBRP]
protocol	Van Kerckvoorde M., Live Imaging and Analysis of Cilia and Cell Cycle Dynamics with the Arl13bCerulean-Fucci2a Biosensor and Fucci Tools. Methods Mol. Biol. 2329: 291-309 (2021). PMID 34085231.