Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-Arl13bCerulean-Fucci2a

Mammalian expression construct of Arl13bCerulean-Fucci2a

Catalog number RDB16059
Resource name pCAG-Arl13bCerulean-Fucci2a
Clone info. Mammalian expression construct containng the CAG promoter drving Arl13bCerulean-Fucci2a a multicistronic version of Fucci2 composed of Arl13bCerulean, mVenus-hGem(1/110) and mCherry-hCdt1(30/120) fused with t2a and p2a.
Comment This clone was used in subcloning pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a by the depositor.
Vector backbone pCAGiP (Plasmid)
Selectable markers Ampicillin
Gene/insert name Human CDT1 cDNA Human Gem cDNA Mouse Arl13b cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Jackson, Ian | Mort, Richard |
Other clones in our bank

External Database
Human CDT1 Human Gem Mouse Arl13b Discosoma sp. RFP
    Aequorea victoria GFP

      Reference sequence
        
       

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions for distribution 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
      2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested.
      3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
      4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
      5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
      6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
      Additional terms and conditions:
      RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
      Ordering Please visit Information of Request for Distribution.[link] 
      Order form 
      [Credit Card Payment]  [Bank Transfer Payment]
      Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
      This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
      Approval form [Word] by Dr. Atsushi Miyawaki
      Remarks
      This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
      Please obtain written approval of Dr. Atsushi Miyawaki.
      提供案内 (日本国内) [open/close]

      提供条件 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
      2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested.
      3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
      4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
      5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
      6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
      付加的提供条件:
      利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
      提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
      提供依頼書 [Word]
      提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
      GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
      宮脇敦史博士による提供承諾書 [Word]
      備考 提供条件の言語は英語のみ。
      Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
      宮脇敦史博士の書面による提供承諾が必要です。

      Catalog # Resource name Shipping form Fee (non-profit org.)
      RDB16059 pCAG-Arl13bCerulean-Fucci2a DNA solution

      Please review the QC test results indicated by check icon below as well as clone information before placing your order.

      References and tips

      Original reference

      original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.

      Further references such as user reports and related articles (go to bottom)

      Featured content

      Featured content Fucci-related materials (en) (English text)
      Featured content Fucci-related materials (ja) (Japanese text)

      QC test results

      RIKEN BRC has sequenced portions of this material for quality test.
      Please review the QC test results indicated by check icon as well as clone information before placing your order.

      Test sheet RDB16059_A9K8p1-1.pdf check

      Nucleotide sequence of a portion of this resource (if available).

      Primer: pCAG-F_V2
      Region: CAG pro, insert 5' (Arl13b 5')
      Sequence file: RDB16059_A9K8a.seq check
      >D05769A4_A9K8_1_pCAG-F_V2_C05_08_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNTG NGCGGNNCCG ANTCTGGGAN GNGCCGCCGC ACCCCCTCTA
         61 GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG GAGGGCCTTC
        121 GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT CCGCGGGGGG
        181 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG
        241 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA
        301 ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAC ACGCGTGCTA
        361 GCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTATGTTCA GTCTGATGGC CAACTGCTGC
        421 AACTTGTTCA AGCGGTGGCG GGAGCCTGTC AGAAAGGTGA CACTTGTGAT GGTCGGCCTT
        481 GATAATGCTG GTAAAACAGC CACAGCAAAG GGAATCCAAG GAGAGCATCC TGAANACGTA
        541 GCTCCCACTG TTGGCTTTTC TAAAATTGAT CTGAGACAAG GAAAGTTCCA AGTTACCATC
        601 TTTGACTTAN GAGGTGGAAA AAGAATTCGG GGAATTTGGA AGAATTATTA TGCTGAATCC
        661 TATGGGGTAA TATTTGTTGT GGATTCCAGT GATGANGAGA GAATGGAAGA AACNAAGGAG
        721 ACAATGTCCG AANTGCTNAG ACACCCGANG ATATCANGAA AGCCTATATT GGTGCTGGNC
        781 AACAAGCAAG ACNAGGNNGG GGNTTTAGGC GAANCGGANG TGATTGANTG NCTCTCGCTG
        841 GANAANCTGG NCNACGAGCA CAANNGNCTG TGTCNNATCN AANCTTGTTC ANCNNTCTTG
        901 GGANATGGAA ANAAAANTGA NNAGNNNNTN NAAAAGGGGC TTTNNTGGNN NNCTNNNNNT
      961 CNTNGNNANN NNNTTTNNNN NCNTNNANNN NNNNCNTCCN NAAAGANNNN NNNNNN
      //
      Primer: mCerulean-N_R
      Region: insert mid (mCerulean, Arl13b 3')
      Sequence file: RDB16059_A9K8b.seq check
      >D05769A4_A9K8_1_mCerulean-N_R_A06_03_ABI24.ab1
          1 NNNNNNNNNN NNGNNNNNNN NNNNGGNNNC TNGCCGGNGG NGCANATGAN CTTCAGGGTC
         61 AGCTTGCCGT ANGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT
        121 ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG
        181 CTCACCATGG TGGCGACCGG TGGATCCCCT GAGATCGTGT CCTGAGCATC ACCGTTAGGT
        241 CTCTGTCGCA CAGCCAGGGG AGGCAGCGGC TTCCCATAGA AATCATTGTG ACGTGTTTCA
        301 CCAAGAGGCT CAAGTTTTGG AAGTCTAGTG ACTTTGGGGG TTCCCCAGCC AACAGGAGGG
        361 GGAGGTTGGG GAGGCGTGGG ACTCTTTGGA GTAGACTCGT CTGTATTGAC TGGTTCTACC
        421 CGATGACTCC TTTTCATTCT TAGTTTCTTG GTTTTCTTCT TACTGTTTTC CCCGGACTCT
        481 GACCCTCGCT GGTCCTGCTC ATCCTCACTG TCCAGCTGCT GCGACAGTGC CTCCCTGTAA
        541 CTGTCCACCA CTCGCTCGTC AGGGTTTTGG AGGCAGCAAT CTGCCTCACT CTGAGGCGCT
        601 GCTTGCTCTG GCTCCACTTT ATGTTCTAGG ANANCAACAT CACTGTCCTT CTCCACGGTC
        661 TGCTTCTTTT TCTCTTTTTC CTGCTTCTTT TCATTCTCAA TGATTACAGC TGCAATGGGT
        721 TGGANAGGAN TCGCAAGAAC TGGTCCTGAN TCAATCTCAG CCAGACCACN NGTCCCATCA
        781 AGTNNCGTCT GCTCTCGTTC TCTTTCTTCC CTTANNNTCC NGANTCGTTC ANCCCNCTCA
        841 CGTTTCTCTN GTNCCNCNNG ANCTCGCNGT TCANTTNNGN NNTTNNGGAT NCNNNCANTT
      901 NNNNNATCNA NNNNCNTNNN NNNNNNN
      //
      Primer: BGH_rev2
      Region: BGH p(A), insert 3' (Gem1_1-110, mVenus)
      Sequence file: RDB16059_A9K8c.seq check
      >D05769A4_A9K8_1_BGH_rev2_B06_06_ABI24.ab1
          1 NNNNNNNNNN NNNNNGNNNN GGGGNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC
         61 AGTCGAGGCT GATCAGCGAG CTCTAGATCA TCGAAGGCGC GCCACATGTG GTACCTTACA
        121 GCGCCTTTCT CCGTTTTTCT GCCACTTCCT TCCAATACTG AGAGGATGGA TTTTCTTTAA
        181 TCATAAGATC AAATGACTCC TGGGTGACTC CTCCAAGATT TTTATTTTCA CTAGATTCTG
        241 GGACAATAAC CCCAGGGCTG GAAGTTGTAG ATGTTAAGTG GTCATTCCGA TGTTTCCTTT
        301 TGGACAAGCC TGCGGACAGC TCATTTTCTC TTCCAACAAG AGATCCAGAT GCAGAAGGCT
        361 GAATCATCTT CAGAGTTCTT CTTGGGACAG AACTATTCTT TATATTCTCT TTGATTTCTT
        421 CTTGTTTCTG CTTCATACTG GGATTCATCT CGAGCGGCCG CCAGTGTGAT GGATATCCCT
        481 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA
        541 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT
        601 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
        661 GCTGCACGCC GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT
        721 TCTGCTTGTC GGCGGTGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC
        781 CCANGATGTT GCCGTCCTCC TTGAANTCGA TGCCCTTCAG CTCGATGCGG TTCANCAGGG
        841 NGTCGCCCNC GAACTTCACC TCNGCGCGGG NNTTGTANTT GCCGNCNNCC TTNNNGAANA
      901 TGGNGCNCTC CNNGNNNNNN NNNN
      //
      Primer: P2A_R2
      Region: insert mid (mCerulean)
      Sequence file: RDB16059_A9K8d.seq check
      >D05769A4_A9K8_1_P2A_R2_E05_14_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNTN NGTAGCTCCG CTTCCCTTGT ACNGCTCGTC CNTGCCGAGA
         61 GTGATCCCGN NNNNNNNNNN NAACTCCAGC AGGACCATGT GATCGCGCTT CTCGNTGGNG
        121 TCTTTGCTCA GCTTGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG
        181 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG
        241 TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC GGTGATATAG
        301 ACGTTGTCGC TGATGGCGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG
        361 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG
        421 GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG GACGTAGCCT
        481 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGGCGAAG
        541 CACTGCACGC CCCAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG
        601 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG CATCGCCCTC GCCCTCGCCG
        661 GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC
        721 CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGNCGA NCGGTGGATC CCCTGAGATC
        781 GTGTCCTGAG CATCACCGTT AGGTCTCTGT CGCACAGCCA GGGGNNGCAG CGGCTTCCCA
        841 TAGAAATCAT TGTGACGTGT TTCACCAAGA GGCTCAAGTT TTGGAAGTCT ANTGANTTTG
        901 GGGGGTTCCC NAGCCAACAG GNNGGGGGAN GTNGGGGANN NGNGGGNNTC TTTGGANTAN
        961 ANTCGNCNGT ANTGANNNGN NNCNNCCCNA TNNNTNCTTT TCNTTCNTNN NTTNNNNNNN
      1021 TTNNNNNNNN NN
      //
      Primer: mCherry-C_R2
      Region: insert mid (mCherry, P2A, mCerulean)
      Sequence file: RDB16059_A9K8e.seq check
      >D05769A4_A9K8_1_mCherry-C_R2_D05_11_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNT TNNGCTTNNG CCTCTGCTTG ATCTCGCCCT TCNGGGCGCC
         61 GTCCTCGGGG TACATCCGCT CGGAGGAGGC CTCCCAGCCC ATGGTCTTCT TCTGCATTAC
        121 GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC TTGTAGATGA ACTCGCCGTC
        181 CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG TCCTCGAAGT TCATCACGCG
        241 CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG TCGGGGATGT CGGCGGGGTG
        301 CTTCACGTAG GCCTTGGAGC CGTACATGAA CTGAGGGGAC AGGATGTCCC AGGCGAAGGG
        361 CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC
        421 CTCGCCCTCG CCCTCGATCT CGAACTCGTG GCCGTTCACG GAGCCCTCCA TGTGCACCTT
        481 GAAGCGCATG AACTCCTTGA TGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATACG
        541 CGCAGGTCCA GGGTTCTCCT CCACGTCTCC AGCCTGCTTC AGCAGGCTGA AGTTAGTAGC
        601 TCCGCTTCCC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC
        661 CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGGGTGCT
        721 CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA
        781 GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTGGNCTT
        841 GATGCCGTTC TTCTGCTTGN CGGCGGTGAT ATAGACGTTG TCGCTGATGG CGTTGTANTC
        901 CNGCTNGTGN CCCNNNATGT TGCCGNCCNN CTTGAAGTCN ANGCCCTNCA NNNCGATGCN
      961 GNNNCACNNG GGNNNCNNCN NNNAACTTCN NNNNNGCNNN GNNNNNNNAN NNNNNNNNN
      //
      Primer: mCherry-M_F1
      Region: insert mid (mCherry, Cdt1_30-120, T2A, mVenus)
      Sequence file: RDB16059_A9K8f.seq check
      >D05769A4_A9K8_1_mCherry-M_F1_B05_05_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNA TGNNNAANNN NNNNTGGGCT GGGNNGCCTC CTCCGAGCGG
         61 ATGTACCCCG AGGNNGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC
        121 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG
        181 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC
        241 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG
        301 TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA GCCCCGCCAG GCCCGCACTC
        361 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC
        421 GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC GGCTGTCGGT GGACGAGGTT
        481 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG
        541 ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC TGACATCCGC GCAGGACCAG
        601 GACACCATCC AATTGGAAGG CCGTGGCTCT CTTCTCACTT GTGGCGATGT CGAAGAGAAT
        661 CCTGGACCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
        721 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGANGGCGAT
        781 GCCACCTACN GCAAGCTGAN CCTNAANCTG ATCTGCACCA CCGGCAAGCT GCCCGNGCCC
        841 TGGCCCACCC TCGNGACCAC CCTGGGCTAC NGNCTGCAGN GNTTCNCCCG CNANCCCNAC
        901 CACNTGAAGC ANCACGACTN CTTCAAGTNC NNCNNGNCCN NNNNNNCNNN CAGNANCGCA
        961 NCATCTNCTN CNNNNANGAN NNNNNNTANN NNANCCNNNN CNANNNNNNN NTNNNNNGNN
      1021 NNNNNCNNNN NNNNANNNNN NNNNN
      //
      Primer: Cdt1_F1
      Region: insert mid (T2A, mVenus)
      Sequence file: RDB16059_A9K8g.seq check
      >D05769A4_A9K8_1_Cdt1_F1_F06_18_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNGG NNNNCNTCCN ATTGGAAGGC CGTGGCTCTC
         61 TTCTCNCTTG TGGCGATGTC GAAGAGAATC CTGGACCCAT GGTGAGCAAG GGCGAGGAGC
        121 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT
        181 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA
        241 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG
        301 GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG
        361 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA
        421 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG
        481 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA
        541 GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA
        601 TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC
        661 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCAAGC
        721 TGANCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGANCGCCG
        781 CCGGGNTCAC TCTCGGCATG NNCGANCTGT ACAANGNNTA TCCATCACAC TGGCGGCCGC
        841 TCNANATGAA TCCNANTATG AAGCANAANN NAAGAAGAAA TCAAAGANNN TATAAAGNNN
      901 ANTTCNGNCC NNANNANANN TCNNNANNAN NNNTNNNNNN NNNN
      //

      Please visit Sequencing and PCR primers for primer information.


      References

      Original, user report and related articles

      original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.
      protocol Van Kerckvoorde M., Live Imaging and Analysis of Cilia and Cell Cycle Dynamics with the Arl13bCerulean-Fucci2a Biosensor and Fucci Tools. Methods Mol. Biol. 2329: 291-309 (2021). PMID 34085231.

      2022.10.12

      GNP_filter3_RDBDEP_html_221010.pl