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pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a

Rosa26 ES cell targeting construct of Arl13bCerulean-Fucci2a.

Catalog number RDB16058
Resource name pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a
Clone info. Rosa26 ES cell targeting construct to insert (by homologous recombination) Arl13bCerulean-Fucci2a (a multicistronic version of Fucci2 composed of Arl13bCerulean, mVenus-hGem(1/110) and mCherry-hCdt1(30/120) fused with t2a and p2a) into the mouse Rosa26 locus.
This clone was used in generating stable ES cell lines by the depositor.
Vector backbone pROSA26 (Plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name Human CDT1 cDNA Human Gem cDNA Mouse Arl13b cDNA Corynebacterium diphtheriae Diphtheria toxin A genomic DNA
Depositor|Developer Jackson, Ian | Mort, Richard |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) | mouse Arl13b (NCBI Gene 68146) | Corynebacterium diphtheriae tox (KEGG orthology K00776) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested.
3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
Ordering Please visit Information of Request for Distribution.[link] Please contact us. UCSD's MTA is also required.
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please contact us. UCSD's MTA is also required.
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16058 pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16058_A9K8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2
Region: CAG pro, loxP, NeoR
Sequence file: RDB16058_A9K8a.seq check
>D05769A3_A9K8_3_pCAG-F_V2_E06_15_ABI24.ab1
    1 NNNNNNNNNN NANNNTGNGC GGNNCCGNNT CTGGGAGGNG CCGCCGCACC CCCTCTAGCG
   61 GGCGCGGGGC GAAGCGGTGC GGCGCCGGCA GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG
  121 CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC TCCAGCCTCG GGGCTGTCCG CGGGGGGACG
  181 GCTGCCTTCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT
  241 CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
  301 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CGATATCAAG CTTATCGAGC
  361 GGCCGCTCTA GAACTAGTGG ATCCATAACT TCGTATAGCA TACATTATAC GAAGTTATCC
  421 CAGATCCCCC GGGCTGCAGC CAATATGGGA TCGGCCATTG AACAAGATGG ATTGCACGCA
  481 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
  541 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC
  601 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG
  661 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG
  721 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT
  781 GCCGANAAAG TATCCATCAT GGCTGANGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
  841 ACCTGCCCAT TCNACCACCA AGCGAANCAT CNNNTCGAGN NNNNNCGTAC TCGGATGGAA
  901 GCCNGNCTTG NCNATCANGA TGNNCTGGNN NAAGANCATC NNNGNCTCGC GCCNGCCNAN
  961 NNNTTCGCNA GGCNCNNNGN NCNCATGCCC NANNNNNANN NNNNCNNCNN NNCCNNNNNN
1021 NATNNNNGNT NNNCNNNNNN NNNN
//
Primer: pAxCALNLF1
Region: insert 5' (loxP, Arl13b 5')
Sequence file: RDB16058_A9K8b.seq check
>D05769A3_A9K8_3_pAxCALNLF1_E02_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCTNNNNN NCCCGATATC CCCATAACTT CGTATAGCAT
   61 ACATTATACG AAGTTATGGA TCCCCCGGGC TGCAGGAATT CCTCGACACG CGTGCTAGCG
  121 CCGCCACCAT GTTCAGTCTG ATGGCCAACT GCTGCAACTT GTTCAAGCGG TGGCGGGAGC
  181 CTGTCAGAAA GGTGACACTT GTGATGGTCG GCCTTGATAA TGCTGGTAAA ACAGCCACAG
  241 CAAAGGGAAT CCAAGGAGAG CATCCTGAAG ACGTAGCTCC CACTGTTGGC TTTTCTAAAA
  301 TTGATCTGAG ACAAGGAAAG TTCCAAGTTA CCATCTTTGA CTTAGGAGGT GGAAAAAGAA
  361 TTCGGGGAAT TTGGAAGAAT TATTATGCTG AATCCTATGG GGTAATATTT GTTGTGGATT
  421 CCAGTGATGA GGAGAGAATG GAAGAAACAA AGGAGACAAT GTCCGAAGTG CTAAGACACC
  481 CGAGGATATC AGGAAAGCCT ATATTGGTGC TGGCCAACAA GCAAGACAAG GAGGGGGCTT
  541 TAGGCGAAGC GGATGTGATT GAGTGTCTCT CGCTGGAGAA GCTGGTCAAC GAGCACAAGT
  601 GCCTGTGTCA GATCGAACCT TGTTCAGCAG TCTTGGGATA TGGAAAGAAA ATTGACAAGT
  661 CCATTAAAAA GGGGCTTTAT TGGCTACTGC ATATCATTGC AAAGGACTTT GATGCCTTAA
  721 GTGAACGCAT CCAGAAAGAC ACAACTGAAC AGCGAGCTCT TGANGAACAA GAGAAACGTG
  781 AGAGGGCTGN NNNNGTCCGG AAGTTAAGGG AAGAANGAGA ACGANAGCAG ACNGAACTTG
  841 ATGNNNCCAG NGGTCTGNNT GANATTGACT CNNNNCAGTT CTTGNGAANN CNTTCNNACC
  901 CATTGCAGCT GTAATCATTG AGAATGAAAA GAAGCNNNAA AAAGANNAAA ANNAANCNGA
  961 NNNNGGANAA NGGNNNNNNG ATGNNGGNCT NNNANNNNAT AAANNNNGNA NCNNGNNNNA
1021 NCANCGNCNT CNNNNNNNNN GNNNNNANNN NNNNNNN
//
Primer: mCerulean-N_R
Region: insert mid (mCerulean, Arl13b 3')
Sequence file: RDB16058_A9K8c.seq check
>D05769A3_A9K8_3_mCerulean-N_R_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN AGCTNGCCGG NGGTGCAGAT GAACTTCNGG
   61 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG
  121 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
  181 TTGCTCACCA TGGTGGCGAC CGGTGGATCC CCTGAGATCG TGTCCTGAGC ATCACCGTTA
  241 GGTCTCTGTC GCACAGCCAG GGGAGGCAGC GGCTTCCCAT AGAAATCATT GTGACGTGTT
  301 TCACCAAGAG GCTCAAGTTT TGGAAGTCTA GTGACTTTGG GGGTTCCCCA GCCAACAGGA
  361 GGGGGAGGTT GGGGAGGCGT GGGACTCTTT GGAGTAGACT CGTCTGTATT GACTGGTTCT
  421 ACCCGATGAC TCCTTTTCAT TCTTAGTTTC TTGGTTTTCT TCTTACTGTT TTCCCCGGAC
  481 TCTGACCCTC GCTGGTCCTG CTCATCCTCA CTGTCCAGCT GCTGCGACAG TGCCTCCCTG
  541 TAACTGTCCA CCACTCGCTC GTCAGGGTTT TGGAGGCAGC AATCTGCCTC ACTCTGAGGC
  601 GCTGCTTGCT CTGGCTCCAC TTTATGTTCT AGGAGACCAA CATCACTGTC CTTCTCCACG
  661 GTCTGCTTCT TTTTCTCTTT TTCCTGCTTC TTTTCATTCT CAATGATTAC AGCTGCNATG
  721 GGTTGGAAAG GATTCGCAAG AACTGGTCCT GAGTCAATCT CAGCCAGACC ACTGGTCCCA
  781 TCAAGTTCCG TCTGCTCTCG TTCTCTTTCT TCCCTTAACT TCCGGACTCG TTCAGCCCTC
  841 TCACGTTTCT CTTGTTCCTC AAGANCTCGC TGTTCAGTTG TGTCTTTCTG GATGCGTTCA
  901 CTNNANNNAT CAAAGNNCTT TGCAATGATA TGCAGTANCC AATNAAGCCC CCTTTTTANT
961 GGANTTGTCN ANTTTTCTTT NCNTATNNNA NNNNTGNNNN NNNNNN
//
Primer: mVenus-M_F
Region: insert 3' (mVenus, Gem1_1-110, BGH p(A))
Sequence file: RDB16058_A9K8d.seq check
>D05769A3_A9K8_3_mVenus-M_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNATC NNGGCCNACT TNNNGNTCCG CCNNCAACAT CGAGGNCGGC
   61 GGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
  121 CTGCCCGACA ACCACTACCT GAGCTACCAG TCCAAGCTGA GCAAAGACCC CAACGAGAAG
  181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
  241 GAGCTGTACA AGGGATATCC ATCACACTGG CGGCCGCTCG AGATGAATCC CAGTATGAAG
  301 CAGAAACAAG AAGAAATCAA AGAGAATATA AAGAATAGTT CTGTCCCAAG AAGAACTCTG
  361 AAGATGATTC AGCCTTCTGC ATCTGGATCT CTTGTTGGAA GAGAAAATGA GCTGTCCGCA
  421 GGCTTGTCCA AAAGGAAACA TCGGAATGAC CACTTAACAT CTACAACTTC CAGCCCTGGG
  481 GTTATTGTCC CAGAATCTAG TGAAAATAAA AATCTTGGAG GAGTCACCCA GGAGTCATTT
  541 GATCTTATGA TTAAAGAAAA TCCATCCTCT CAGTATTGGA AGGAAGTGGC AGAAAAACGG
  601 AGAAAGGCGC TGTAAGGTAC CACATGTGGC GCGCGCGCCT TCGATGATCT AGAGCTCGCT
  661 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC
  721 CTTCCTTGAC CCTGGAANGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GANGAAATTG
  781 CATCGCATTG TCTGANTANG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CANGNCAGCA
  841 AGGGGGGAGG ATTGGGAAGA NAATAGCAGG CATGCNGGGG GATGCNGNGG GCNCTATGGC
  901 TTCTGANGCG GAAAGAANCN GCNNCCCNNN CNNGGGCNGN NGGGNNNNNC CNANNNNNNN
961 NNN
//
Primer: P2A_R2
Region: insert mid (mCerulean)
Sequence file: RDB16058_A9K8e.seq check
>D05769A3_A9K8_3_P2A_R2_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTAGCTCCGC TTCCCTTGTA CNGCTCGTCC NTGCCGAGAG
   61 TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT
  121 CTTTGCTCAG CTTGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT
  181 CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT
  241 TGTGGCGGAT CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA
  301 CGTTGTCGCT GATGGCGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA
  361 AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG
  421 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT
  481 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC
  541 ACTGCACGCC CCAGGTCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG
  601 TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG
  661 ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC
  721 CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGAN CGGTGGATCC CCTGANATCG
  781 TGTCCTGAGC ATCACCGTTA GGTCTCTGTC GCACAGCCAG GGGANGCAGC GGCTTCCCAT
  841 ANAAATCATT GTGACGTGTT TCACCNAGAN GNTCANGTTT TGGAAGTCNA GTGANTTTGG
  901 GGGTTCCCCA GCCNACAGGA NGGGGANGNT GGGGNANGNG NNGGNNTCTT NNGANTANNN
  961 TCNNCNGNAN TGANNNNNNN NACCNNATGA NTCCTTTNCN TTCNNANNTT NNNNNNNNNN
//
Primer: FP-C_F1
Region: insert mid (mCerulean, P2A, mCherry)
Sequence file: RDB16058_A9K8f.seq check
>D05769A3_A9K8_3_FP-C_F1_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCGCGNTCNC NTGGTCCTGC TGGNGTTCGT GNCCGCCGCC
   61 GGGNTCACTC TCGGCATGGA CGAGCTGTAC AAGGGAAGCG GAGCTACTAA CTTCAGCCTG
  121 CTGAAGCAGG CTGGAGACGT GGAGGAGAAC CCTGGACCTG CGCGTATGGT GAGCAAGGGC
  181 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC
  241 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC
  301 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC
  361 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC
  421 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG
  481 GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC
  541 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGANC
  601 ATGGGCTGGG ANGNCTCCTC CGAGCGGATG TACCCCGAGG ANGGCGCCNT GAANGGCGAG
  661 ATCAAGCAGA NGCTGAAGCT GAANGANGGC GGCCACTACG ACGCTGANGT CAAGACCACC
  721 TACNAGGNCN AGAANCCCGT GCAGCTGCCC GGCGCCTACN NCGTCNACNT CAAGTTGGNN
  781 NTNACNTNCC NCNANNAGGA NTNCNCCATC NNNNNNNAGT ACNAANGCGC CNANGGNNGN
841 NNNTNCNNCN GNGNNNTNNN NNANNNNNNN NNN
//
Primer: mCherry-M_F1
Region: insert mid (mCherry, Cdt1_30-120, T2A, mVenus)
Sequence file: RDB16058_A9K8g.seq check
>D05769A3_A9K8_3_mCherry-M_F1_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNA TGNNGAANAN NNCNTGGGCT GGGANGCCTC CTCCGAGCGG
   61 ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC
  121 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG
  181 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC
  241 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG
  301 TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA GCCCCGCCAG GCCCGCACTC
  361 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC
  421 GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC GGCTGTCGGT GGACGAGGTT
  481 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG
  541 ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC TGACATCCGC GCAGGACCAG
  601 GACACCATCC AATTGGAAGG CCGTGGCTCT CTTCTCACTT GTGGCGATGT CGAAGAGAAT
  661 CCTGGACCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
  721 GAGCTGGACG GCGACGTNNN NNGCCACAAG TTCAGCGTGT CCGGCGAGGG CGANGGCGAT
  781 GCCACCTACG GCAAGCTGAN CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCNCGTGCCC
  841 NGGCCCACCN TCGNGACCAC CCNGGGCTAC NGCCTGCAGN GCTTCGCCCG CTANCCCGAN
  901 CACATGAAGC AGCNCGACTT CTTCNAGNNC GNNNTGNCCN NNNNNTACGT CCANNANCGC
961 ANNATCNTNN TNNNNNANNA NNGNNNNTAN NNNNCNNNNN NNNNN
//
Primer: Cdt1_F1
Region: insert mid(T2A, mVenus)
Sequence file: RDB16058_A9K8h.seq check
>D05769A3_A9K8_3_Cdt1_F1_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNNCCNTCN NATTGGNAGG CCGTGGCTCT
   61 CTTCTCACTT GTGGCGATGT CGAAGAGAAT CCTGGACCCA TGGTGAGCAA GGGCGAGGAG
  121 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG
  181 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG
  241 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC
  301 GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC
  361 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC
  421 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG
  481 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC
  541 AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG
  601 ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC
  661 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGANCTA CCAGTCCAAG
  721 CTGANCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGANTT CGTGACCGCC
  781 GCCGGGATCA CTCTCGGCAT GGACGANCTG TACNAGGGAT ATCCATCACA CNGGCGGCCG
  841 CTCGAGATGA ATCCCAGTAT GAAGCANAAA CNAGAANAAA TCAAAGAGAA TATAAAGAAT
901 AGTTCTNNCC NNNNNAGANN NCNGNANNAN GANTNNAGCC TTCNNNNNNN NNN
//
Primer: PGKpro_R
Region: PGK pro, Rosa26 right arm
Sequence file: RDB16058_A9K8i.seq check
>D05769A3_A9K8_3_PGKpro_R_H06_24_ABI24.ab1
    1 NNNNNNNNNN NGNNNNNGAN GNGTGCGAGG CCNGANGCCA CTTGTGTAGC GCCNAGTGCC
   61 CAGCGGGGCT GCTAAAGCGC ATGCTCCAGA CTGCCTTGGG AAAAGCGCCT CCCCTACCCG
  121 GTAGAATTCG ATATCAAGCT TATCGATACC GTCGACGGTA TCGATAAGCT AGATGTCCTG
  181 AAATATTTCT ATCAGAACAA GGTAGTATAA AGCTGGTAGG TATACAAAAC GCTAGACTAG
  241 TTTCTATCCC TGACCCTTAA TCTGCTAGTA TATCCGTAGG AAGTTGCTTA AGTGCCACTA
  301 GTACCAACAG CCTCTGTTAG AGTGAACAGT AGATTTTAAT GAAGGGCCAA TAAAGACCAC
  361 TTAAGACTAC TGACTGAATT AAAAATCCAC CCAGTGGAGT TAGTATTGGT TATAGTTATA
  421 TAACAAAATA TCCAACTTAG CCAGGCGTGG TGGTGCACGC CTTTAATCCC GGCACTCAGG
  481 AGGCAGAGGC AGGCGGATTT CTGAGTTCGA GGCCAGCCTG GTCTACAAAG TGAGTTCCAG
  541 GACAGCCAGG GCTATACAGA GAAACCCTGT CTCGAAAAAC CAAAAAAAAA AAAAAAAAGG
  601 AAAAAAAATA TGGCTTGAAA ACATGCTATT GTTGTCAAAA GGAGATCCAA ACAAGTATTT
  661 CTTTGAACAA AAATTATGTA GAAAACAGGG TGATAAAGAA CAAGGGCTTT TTAAAAAAGG
  721 NCAGCATTAT TTGAGATATA CAAGTGACTC CNATTCAAGC CTAAAGGNCA CTCAATGCTC
  781 ACTAACAGTG CTAATCANAA TCAATTCCTT ANCCCAGTCN AAAATAGTCC TGAATATCTT
  841 CTGGANAAAC NTGTCCTCCN ACATATAANC TAGTATACAC TAACAAAANG TCTCNACTTC
  901 AANGNGAAAT GCTTGANTCC TANANTTGNG NNCCANCAAA GNGCTCTATN NNNAGGNNTA
  961 NTNNNTCANA NNNNNTNGNN NGNNANNNNN NNNTANNNNN TNNCNNNNAN NGNNNNNNNN
1021 GNNAAANNNN NNNNNTTNNN NNNNNNN
//
Primer: pUC-M13_-100
Region: Rosa26 left arm
Sequence file: RDB16058_A9K8j.seq check
>D05769A3_A9K8_3_pUC-M13_-100_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGNGNANG CCNGGGTTTT CCCNGTCNCG ACGTTGNNNN
   61 NNGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCACCGC
  121 GGCAGGCCCT CCGAGCGTGG TGGAGCCGTT CTGTGAGACA GCCGGGTACG AGTCGTGACG
  181 CTGGAAGGGG CAAGCGGGTG GTGGGCAGGA ATGCGGTCCG CCCTGCAGCA ACCGGAGGGG
  241 GAGGGAGAAG GGAGCGGAAA AGTCTCCACC GGACGCGGCC ATGGCTCGGG GGGGGGGGGG
  301 CAGNGNANGN NNNCTTCCGG NCNANNTCTC NNCNNTGATT GNNTTCTTTT CCTCCCGCCN
  361 NGNNNGAAAA CACNANTGGN NNGTTTTGNT TGGNGTAAGG CNCCTGNCAN TTAACGGCAN
  421 CNNNANNGNN CANCCGCCGG CNNCCTCNCT CTGCCNNNNG NNNGGGGCGG NAGGNAGGTG
481 GGGNGAGGNN ANNTGNANNN GCGGGNNCGG TCGNCNNNNG GCGGGGCGGN NNANGNNN
//
Primer: PGKpro_F
Region: DTA, small t intron
Sequence file: RDB16058_A9K8k.seq check
>D05769A3_A9K8_3_PGKpro_F_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNNNNNNCNG GCCTTTCGAN CTGCAGGTCC TCGCCATGGN
   61 TCCTGATGAT GTTGTTGATT CTTCTAAATC TTTTGTGATG GAAAACTTTT CTTCGTACCA
  121 CGGGACTAAA CCTGGTTATG TAGATTCCAT TCAAAAAGGT ATACAAAAGC CAAAATCTGG
  181 TACACAAGGA AATTATGACG ATGATTGGAA AGGGTTTTAT AGTACCGACA ATAAATACGA
  241 CGCTGCGGGA TACTCTGTAG ATAATGAAAA CCCGCTCTCT GGAAAAGCTG GAGGCGTGGT
  301 CAAAGTGACG TATCCAGGAC TGACGAAGGT TCTCGCACTA AAAGTGGATA ATGCCGAAAC
  361 TATTAAGAAA GAGTTAGGTT TAAGTCTCAC TGAACCGTTG ATGGAGCAAG TCGGAACGGA
  421 AGAGTTTATC AAAAGGTTCG GTGATGGTGC TTCGCGTGTA GTGCTCAGCC TTCCCTTCGC
  481 TGAGGGGAGT TCTAGCGTTG AATATATTAA TAACTGGGAA CAGGCGAAAG CGTTAAGCGT
  541 AGAACTTGAG ATTAATTTTG AAACCCGTGG AAAACGTGGC CAAGATGCGA TGTATGAGTA
  601 TATGGCTCAA GCCTGTGCAG GAAATCGTGT CAGGCGATCT CTTTGTGAAG GAACCTTACT
  661 TCTGTGGTGT GACATAATTG GACAAACTAC CTACAGAGAT TTAAAGCTCT AAGGTAAATA
  721 TAAAATTTTT AAGTGTATAA TGTGTTAAAC TACTGATTCT AATTGTTTGT GTATTTTAGA
  781 TTCCNACCTA TGGAACTGAT GAATGGGANC AGNGGTGGAA TGCAGATCCT ANAGCTCGCT
  841 GATCAGCCTC NACTGTGCCT TCTANNTGCC NGCCATCNGN TGTTTGCCCC CNNCCCCCNN
  901 GCCTNNCTTG ANCCTNNAAN GNGNCNCTCC NNNNNNNCNT TNNCTNNNNA ANNNNNNNAN
961 NTNGNNTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.

2020.10.02

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