Please review the QC test results indicated by icon below as well as clone information before placing your order.

pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a

Rosa26 ES cell targeting construct of Arl13bCerulean-Fucci2a.

Sequence (full)	RDB16058zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. 6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. 6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	タンパク質毒素遺伝子です (diphtheria toxin A subunit)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16058_A9K8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 (Pr0330) Region: CAG pro, loxP, NeoR Sequence file: RDB16058_A9K8a.seq
>D05769A3_A9K8_3_pCAG-F_V2_E06_15_ABI24.ab1     1 NNNNNNNNNN NANNNTGNGC GGNNCCGNNT CTGGGAGGNG CCGCCGCACC CCCTCTAGCG    61 GGCGCGGGGC GAAGCGGTGC GGCGCCGGCA GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG   121 CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC TCCAGCCTCG GGGCTGTCCG CGGGGGGACG   181 GCTGCCTTCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT   241 CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG   301 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CGATATCAAG CTTATCGAGC   361 GGCCGCTCTA GAACTAGTGG ATCCATAACT TCGTATAGCA TACATTATAC GAAGTTATCC   421 CAGATCCCCC GGGCTGCAGC CAATATGGGA TCGGCCATTG AACAAGATGG ATTGCACGCA   481 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC   541 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC   601 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG   661 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG   721 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT   781 GCCGANAAAG TATCCATCAT GGCTGANGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT   841 ACCTGCCCAT TCNACCACCA AGCGAANCAT CNNNTCGAGN NNNNNCGTAC TCGGATGGAA   901 GCCNGNCTTG NCNATCANGA TGNNCTGGNN NAAGANCATC NNNGNCTCGC GCCNGCCNAN   961 NNNTTCGCNA GGCNCNNNGN NCNCATGCCC NANNNNNANN NNNNCNNCNN NNCCNNNNNN 1021 NATNNNNGNT NNNCNNNNNN NNNN //
Primer: pAxCALNLF1 (Pr0166) Region: insert 5' (loxP, Arl13b 5') Sequence file: RDB16058_A9K8b.seq
>D05769A3_A9K8_3_pAxCALNLF1_E02_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCTNNNNN NCCCGATATC CCCATAACTT CGTATAGCAT    61 ACATTATACG AAGTTATGGA TCCCCCGGGC TGCAGGAATT CCTCGACACG CGTGCTAGCG   121 CCGCCACCAT GTTCAGTCTG ATGGCCAACT GCTGCAACTT GTTCAAGCGG TGGCGGGAGC   181 CTGTCAGAAA GGTGACACTT GTGATGGTCG GCCTTGATAA TGCTGGTAAA ACAGCCACAG   241 CAAAGGGAAT CCAAGGAGAG CATCCTGAAG ACGTAGCTCC CACTGTTGGC TTTTCTAAAA   301 TTGATCTGAG ACAAGGAAAG TTCCAAGTTA CCATCTTTGA CTTAGGAGGT GGAAAAAGAA   361 TTCGGGGAAT TTGGAAGAAT TATTATGCTG AATCCTATGG GGTAATATTT GTTGTGGATT   421 CCAGTGATGA GGAGAGAATG GAAGAAACAA AGGAGACAAT GTCCGAAGTG CTAAGACACC   481 CGAGGATATC AGGAAAGCCT ATATTGGTGC TGGCCAACAA GCAAGACAAG GAGGGGGCTT   541 TAGGCGAAGC GGATGTGATT GAGTGTCTCT CGCTGGAGAA GCTGGTCAAC GAGCACAAGT   601 GCCTGTGTCA GATCGAACCT TGTTCAGCAG TCTTGGGATA TGGAAAGAAA ATTGACAAGT   661 CCATTAAAAA GGGGCTTTAT TGGCTACTGC ATATCATTGC AAAGGACTTT GATGCCTTAA   721 GTGAACGCAT CCAGAAAGAC ACAACTGAAC AGCGAGCTCT TGANGAACAA GAGAAACGTG   781 AGAGGGCTGN NNNNGTCCGG AAGTTAAGGG AAGAANGAGA ACGANAGCAG ACNGAACTTG   841 ATGNNNCCAG NGGTCTGNNT GANATTGACT CNNNNCAGTT CTTGNGAANN CNTTCNNACC   901 CATTGCAGCT GTAATCATTG AGAATGAAAA GAAGCNNNAA AAAGANNAAA ANNAANCNGA   961 NNNNGGANAA NGGNNNNNNG ATGNNGGNCT NNNANNNNAT AAANNNNGNA NCNNGNNNNA 1021 NCANCGNCNT CNNNNNNNNN GNNNNNANNN NNNNNNN //
Primer: mCerulean-N_R (Pr0770) Region: insert mid (mCerulean, Arl13b 3') Sequence file: RDB16058_A9K8c.seq
>D05769A3_A9K8_3_mCerulean-N_R_F02_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN AGCTNGCCGG NGGTGCAGAT GAACTTCNGG    61 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG   121 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC   181 TTGCTCACCA TGGTGGCGAC CGGTGGATCC CCTGAGATCG TGTCCTGAGC ATCACCGTTA   241 GGTCTCTGTC GCACAGCCAG GGGAGGCAGC GGCTTCCCAT AGAAATCATT GTGACGTGTT   301 TCACCAAGAG GCTCAAGTTT TGGAAGTCTA GTGACTTTGG GGGTTCCCCA GCCAACAGGA   361 GGGGGAGGTT GGGGAGGCGT GGGACTCTTT GGAGTAGACT CGTCTGTATT GACTGGTTCT   421 ACCCGATGAC TCCTTTTCAT TCTTAGTTTC TTGGTTTTCT TCTTACTGTT TTCCCCGGAC   481 TCTGACCCTC GCTGGTCCTG CTCATCCTCA CTGTCCAGCT GCTGCGACAG TGCCTCCCTG   541 TAACTGTCCA CCACTCGCTC GTCAGGGTTT TGGAGGCAGC AATCTGCCTC ACTCTGAGGC   601 GCTGCTTGCT CTGGCTCCAC TTTATGTTCT AGGAGACCAA CATCACTGTC CTTCTCCACG   661 GTCTGCTTCT TTTTCTCTTT TTCCTGCTTC TTTTCATTCT CAATGATTAC AGCTGCNATG   721 GGTTGGAAAG GATTCGCAAG AACTGGTCCT GAGTCAATCT CAGCCAGACC ACTGGTCCCA   781 TCAAGTTCCG TCTGCTCTCG TTCTCTTTCT TCCCTTAACT TCCGGACTCG TTCAGCCCTC   841 TCACGTTTCT CTTGTTCCTC AAGANCTCGC TGTTCAGTTG TGTCTTTCTG GATGCGTTCA   901 CTNNANNNAT CAAAGNNCTT TGCAATGATA TGCAGTANCC AATNAAGCCC CCTTTTTANT 961 GGANTTGTCN ANTTTTCTTT NCNTATNNNA NNNNTGNNNN NNNNNN //
Primer: mVenus-M_F (Pr0574) Region: insert 3' (mVenus, Gem1_1-110, BGH p(A)) Sequence file: RDB16058_A9K8d.seq
>D05769A3_A9K8_3_mVenus-M_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNATC NNGGCCNACT TNNNGNTCCG CCNNCAACAT CGAGGNCGGC    61 GGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG   121 CTGCCCGACA ACCACTACCT GAGCTACCAG TCCAAGCTGA GCAAAGACCC CAACGAGAAG   181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC   241 GAGCTGTACA AGGGATATCC ATCACACTGG CGGCCGCTCG AGATGAATCC CAGTATGAAG   301 CAGAAACAAG AAGAAATCAA AGAGAATATA AAGAATAGTT CTGTCCCAAG AAGAACTCTG   361 AAGATGATTC AGCCTTCTGC ATCTGGATCT CTTGTTGGAA GAGAAAATGA GCTGTCCGCA   421 GGCTTGTCCA AAAGGAAACA TCGGAATGAC CACTTAACAT CTACAACTTC CAGCCCTGGG   481 GTTATTGTCC CAGAATCTAG TGAAAATAAA AATCTTGGAG GAGTCACCCA GGAGTCATTT   541 GATCTTATGA TTAAAGAAAA TCCATCCTCT CAGTATTGGA AGGAAGTGGC AGAAAAACGG   601 AGAAAGGCGC TGTAAGGTAC CACATGTGGC GCGCGCGCCT TCGATGATCT AGAGCTCGCT   661 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC   721 CTTCCTTGAC CCTGGAANGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GANGAAATTG   781 CATCGCATTG TCTGANTANG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CANGNCAGCA   841 AGGGGGGAGG ATTGGGAAGA NAATAGCAGG CATGCNGGGG GATGCNGNGG GCNCTATGGC   901 TTCTGANGCG GAAAGAANCN GCNNCCCNNN CNNGGGCNGN NGGGNNNNNC CNANNNNNNN 961 NNN //
Primer: P2A_R2 (Pr0794) Region: insert mid (mCerulean) Sequence file: RDB16058_A9K8e.seq
>D05769A3_A9K8_3_P2A_R2_G06_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTAGCTCCGC TTCCCTTGTA CNGCTCGTCC NTGCCGAGAG    61 TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT   121 CTTTGCTCAG CTTGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT   181 CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT   241 TGTGGCGGAT CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA   301 CGTTGTCGCT GATGGCGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA   361 AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG   421 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT   481 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC   541 ACTGCACGCC CCAGGTCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG   601 TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG   661 ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC   721 CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGAN CGGTGGATCC CCTGANATCG   781 TGTCCTGAGC ATCACCGTTA GGTCTCTGTC GCACAGCCAG GGGANGCAGC GGCTTCCCAT   841 ANAAATCATT GTGACGTGTT TCACCNAGAN GNTCANGTTT TGGAAGTCNA GTGANTTTGG   901 GGGTTCCCCA GCCNACAGGA NGGGGANGNT GGGGNANGNG NNGGNNTCTT NNGANTANNN   961 TCNNCNGNAN TGANNNNNNN NACCNNATGA NTCCTTTNCN TTCNNANNTT NNNNNNNNNN //
Primer: FP-C_F1 (Pr0772) Region: insert mid (mCerulean, P2A, mCherry) Sequence file: RDB16058_A9K8f.seq
>D05769A3_A9K8_3_FP-C_F1_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCGCGNTCNC NTGGTCCTGC TGGNGTTCGT GNCCGCCGCC    61 GGGNTCACTC TCGGCATGGA CGAGCTGTAC AAGGGAAGCG GAGCTACTAA CTTCAGCCTG   121 CTGAAGCAGG CTGGAGACGT GGAGGAGAAC CCTGGACCTG CGCGTATGGT GAGCAAGGGC   181 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC   241 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC   301 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC   361 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC   421 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG   481 GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC   541 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGANC   601 ATGGGCTGGG ANGNCTCCTC CGAGCGGATG TACCCCGAGG ANGGCGCCNT GAANGGCGAG   661 ATCAAGCAGA NGCTGAAGCT GAANGANGGC GGCCACTACG ACGCTGANGT CAAGACCACC   721 TACNAGGNCN AGAANCCCGT GCAGCTGCCC GGCGCCTACN NCGTCNACNT CAAGTTGGNN   781 NTNACNTNCC NCNANNAGGA NTNCNCCATC NNNNNNNAGT ACNAANGCGC CNANGGNNGN 841 NNNTNCNNCN GNGNNNTNNN NNANNNNNNN NNN //
Primer: mCherry-M_F1 (Pr0767) Region: insert mid (mCherry, Cdt1_30-120, T2A, mVenus) Sequence file: RDB16058_A9K8g.seq
>D05769A3_A9K8_3_mCherry-M_F1_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNGNNNNNA TGNNGAANAN NNCNTGGGCT GGGANGCCTC CTCCGAGCGG    61 ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC   121 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG   181 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC   241 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG   301 TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA GCCCCGCCAG GCCCGCACTC   361 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC   421 GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC GGCTGTCGGT GGACGAGGTT   481 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG   541 ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC TGACATCCGC GCAGGACCAG   601 GACACCATCC AATTGGAAGG CCGTGGCTCT CTTCTCACTT GTGGCGATGT CGAAGAGAAT   661 CCTGGACCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC   721 GAGCTGGACG GCGACGTNNN NNGCCACAAG TTCAGCGTGT CCGGCGAGGG CGANGGCGAT   781 GCCACCTACG GCAAGCTGAN CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCNCGTGCCC   841 NGGCCCACCN TCGNGACCAC CCNGGGCTAC NGCCTGCAGN GCTTCGCCCG CTANCCCGAN   901 CACATGAAGC AGCNCGACTT CTTCNAGNNC GNNNTGNCCN NNNNNTACGT CCANNANCGC 961 ANNATCNTNN TNNNNNANNA NNGNNNNTAN NNNNCNNNNN NNNNN //
Primer: Cdt1_F1 (Pr0768) Region: insert mid(T2A, mVenus) Sequence file: RDB16058_A9K8h.seq
>D05769A3_A9K8_3_Cdt1_F1_D05_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNNCCNTCN NATTGGNAGG CCGTGGCTCT    61 CTTCTCACTT GTGGCGATGT CGAAGAGAAT CCTGGACCCA TGGTGAGCAA GGGCGAGGAG   121 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG   181 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG   241 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC   301 GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC   361 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC   421 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG   481 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC   541 AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG   601 ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC   661 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGANCTA CCAGTCCAAG   721 CTGANCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGANTT CGTGACCGCC   781 GCCGGGATCA CTCTCGGCAT GGACGANCTG TACNAGGGAT ATCCATCACA CNGGCGGCCG   841 CTCGAGATGA ATCCCAGTAT GAAGCANAAA CNAGAANAAA TCAAAGAGAA TATAAAGAAT 901 AGTTCTNNCC NNNNNAGANN NCNGNANNAN GANTNNAGCC TTCNNNNNNN NNN //
Primer: PGKpro_R (Pr0793) Region: PGK pro, Rosa26 right arm Sequence file: RDB16058_A9K8i.seq
>D05769A3_A9K8_3_PGKpro_R_H06_24_ABI24.ab1     1 NNNNNNNNNN NGNNNNNGAN GNGTGCGAGG CCNGANGCCA CTTGTGTAGC GCCNAGTGCC    61 CAGCGGGGCT GCTAAAGCGC ATGCTCCAGA CTGCCTTGGG AAAAGCGCCT CCCCTACCCG   121 GTAGAATTCG ATATCAAGCT TATCGATACC GTCGACGGTA TCGATAAGCT AGATGTCCTG   181 AAATATTTCT ATCAGAACAA GGTAGTATAA AGCTGGTAGG TATACAAAAC GCTAGACTAG   241 TTTCTATCCC TGACCCTTAA TCTGCTAGTA TATCCGTAGG AAGTTGCTTA AGTGCCACTA   301 GTACCAACAG CCTCTGTTAG AGTGAACAGT AGATTTTAAT GAAGGGCCAA TAAAGACCAC   361 TTAAGACTAC TGACTGAATT AAAAATCCAC CCAGTGGAGT TAGTATTGGT TATAGTTATA   421 TAACAAAATA TCCAACTTAG CCAGGCGTGG TGGTGCACGC CTTTAATCCC GGCACTCAGG   481 AGGCAGAGGC AGGCGGATTT CTGAGTTCGA GGCCAGCCTG GTCTACAAAG TGAGTTCCAG   541 GACAGCCAGG GCTATACAGA GAAACCCTGT CTCGAAAAAC CAAAAAAAAA AAAAAAAAGG   601 AAAAAAAATA TGGCTTGAAA ACATGCTATT GTTGTCAAAA GGAGATCCAA ACAAGTATTT   661 CTTTGAACAA AAATTATGTA GAAAACAGGG TGATAAAGAA CAAGGGCTTT TTAAAAAAGG   721 NCAGCATTAT TTGAGATATA CAAGTGACTC CNATTCAAGC CTAAAGGNCA CTCAATGCTC   781 ACTAACAGTG CTAATCANAA TCAATTCCTT ANCCCAGTCN AAAATAGTCC TGAATATCTT   841 CTGGANAAAC NTGTCCTCCN ACATATAANC TAGTATACAC TAACAAAANG TCTCNACTTC   901 AANGNGAAAT GCTTGANTCC TANANTTGNG NNCCANCAAA GNGCTCTATN NNNAGGNNTA   961 NTNNNTCANA NNNNNTNGNN NGNNANNNNN NNNTANNNNN TNNCNNNNAN NGNNNNNNNN 1021 GNNAAANNNN NNNNNTTNNN NNNNNNN //
Primer: pUC-M13_-100 (Pr0310) Region: Rosa26 left arm Sequence file: RDB16058_A9K8j.seq
>D05769A3_A9K8_3_pUC-M13_-100_F05_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNGNANG CCNGGGTTTT CCCNGTCNCG ACGTTGNNNN    61 NNGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCACCGC   121 GGCAGGCCCT CCGAGCGTGG TGGAGCCGTT CTGTGAGACA GCCGGGTACG AGTCGTGACG   181 CTGGAAGGGG CAAGCGGGTG GTGGGCAGGA ATGCGGTCCG CCCTGCAGCA ACCGGAGGGG   241 GAGGGAGAAG GGAGCGGAAA AGTCTCCACC GGACGCGGCC ATGGCTCGGG GGGGGGGGGG   301 CAGNGNANGN NNNCTTCCGG NCNANNTCTC NNCNNTGATT GNNTTCTTTT CCTCCCGCCN   361 NGNNNGAAAA CACNANTGGN NNGTTTTGNT TGGNGTAAGG CNCCTGNCAN TTAACGGCAN   421 CNNNANNGNN CANCCGCCGG CNNCCTCNCT CTGCCNNNNG NNNGGGGCGG NAGGNAGGTG 481 GGGNGAGGNN ANNTGNANNN GCGGGNNCGG TCGNCNNNNG GCGGGGCGGN NNANGNNN //
Primer: PGKpro_F (Pr0249) Region: DTA, small t intron Sequence file: RDB16058_A9K8k.seq
>D05769A3_A9K8_3_PGKpro_F_G05_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC NNNNNNNCNG GCCTTTCGAN CTGCAGGTCC TCGCCATGGN    61 TCCTGATGAT GTTGTTGATT CTTCTAAATC TTTTGTGATG GAAAACTTTT CTTCGTACCA   121 CGGGACTAAA CCTGGTTATG TAGATTCCAT TCAAAAAGGT ATACAAAAGC CAAAATCTGG   181 TACACAAGGA AATTATGACG ATGATTGGAA AGGGTTTTAT AGTACCGACA ATAAATACGA   241 CGCTGCGGGA TACTCTGTAG ATAATGAAAA CCCGCTCTCT GGAAAAGCTG GAGGCGTGGT   301 CAAAGTGACG TATCCAGGAC TGACGAAGGT TCTCGCACTA AAAGTGGATA ATGCCGAAAC   361 TATTAAGAAA GAGTTAGGTT TAAGTCTCAC TGAACCGTTG ATGGAGCAAG TCGGAACGGA   421 AGAGTTTATC AAAAGGTTCG GTGATGGTGC TTCGCGTGTA GTGCTCAGCC TTCCCTTCGC   481 TGAGGGGAGT TCTAGCGTTG AATATATTAA TAACTGGGAA CAGGCGAAAG CGTTAAGCGT   541 AGAACTTGAG ATTAATTTTG AAACCCGTGG AAAACGTGGC CAAGATGCGA TGTATGAGTA   601 TATGGCTCAA GCCTGTGCAG GAAATCGTGT CAGGCGATCT CTTTGTGAAG GAACCTTACT   661 TCTGTGGTGT GACATAATTG GACAAACTAC CTACAGAGAT TTAAAGCTCT AAGGTAAATA   721 TAAAATTTTT AAGTGTATAA TGTGTTAAAC TACTGATTCT AATTGTTTGT GTATTTTAGA   781 TTCCNACCTA TGGAACTGAT GAATGGGANC AGNGGTGGAA TGCAGATCCT ANAGCTCGCT   841 GATCAGCCTC NACTGTGCCT TCTANNTGCC NGCCATCNGN TGTTTGCCCC CNNCCCCCNN   901 GCCTNNCTTG ANCCTNNAAN GNGNCNCTCC NNNNNNNCNT TNNCTNNNNA ANNNNNNNAN 961 NTNGNNTNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl