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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a

Rosa26 ES cell targeting construct of Arl13bCerulean-Fucci2a.

Catalog number RDB16058
Resource name pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a
Clone info. Rosa26 ES cell targeting construct to insert (by homologous recombination) Arl13bCerulean-Fucci2a (a multicistronic version of Fucci2 composed of Arl13bCerulean, mVenus-hGem(1/110) and mCherry-hCdt1(30/120) fused with t2a and p2a) into the mouse Rosa26 locus.
Comment This clone was used in generating stable ES cell lines by the depositor.
Vector backbone pROSA26 (Plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name Human CDT1 cDNA Human Gem cDNA Mouse Arl13b cDNA Corynebacterium diphtheriae Diphtheria toxin A genomic DNA
Depositor|Developer Jackson, Ian | Mort, Richard |
Other clones in our bank

External Database
Human CDT1 Human Gem Mouse Arl13b Corynebacterium diphtheriae Diphtheria toxin A

    Reference sequence
      
     
    Sequence (full) RDB16058zzk02.seq provided by the depositor

    Distribution information

    Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
    Terms and conditions for distribution 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested.
    3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
    4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
    6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
    Additional terms and conditions:
    RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
    Ordering Please visit Information of Request for Distribution.[link] 
    Order form 
    [Credit Card Payment]  [Bank Transfer Payment]
    Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
    This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
    Approval form [Word] by Dr. Atsushi Miyawaki
    Remarks
    This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
    Please obtain written approval of Dr. Atsushi Miyawaki.
    提供案内 (日本国内) [open/close]

    提供条件 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested.
    3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
    4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
    6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
    付加的提供条件:
    利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
    提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
    提供依頼書 [Word]
    提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
    GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
    宮脇敦史博士による提供承諾書 [Word]
    備考 タンパク質毒素遺伝子です (diphtheria toxin A subunit)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。提供条件の言語は英語のみ。
    Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
    宮脇敦史博士の書面による提供承諾が必要です。

    Catalog # Resource name Shipping form Fee (non-profit org.)
    RDB16058 pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a DNA solution

    Please review the QC test results indicated by check icon below as well as clone information before placing your order.

    References and tips

    Original reference

    original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.

    Further references such as user reports and related articles (go to bottom)

    Featured content

    Featured content Fucci-related materials (en) (English text)
    Featured content Fucci-related materials (ja) (Japanese text)

    QC test results

    RIKEN BRC has sequenced portions of this material for quality test.
    Please review the QC test results indicated by check icon as well as clone information before placing your order.

    Test sheet RDB16058_A9K8p1-2.pdf check

    Nucleotide sequence of a portion of this resource (if available).

    Primer: pCAG-F_V2
    Region: CAG pro, loxP, NeoR
    Sequence file: RDB16058_A9K8a.seq check
    >D05769A3_A9K8_3_pCAG-F_V2_E06_15_ABI24.ab1
        1 NNNNNNNNNN NANNNTGNGC GGNNCCGNNT CTGGGAGGNG CCGCCGCACC CCCTCTAGCG
       61 GGCGCGGGGC GAAGCGGTGC GGCGCCGGCA GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG
      121 CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC TCCAGCCTCG GGGCTGTCCG CGGGGGGACG
      181 GCTGCCTTCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT
      241 CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
      301 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CGATATCAAG CTTATCGAGC
      361 GGCCGCTCTA GAACTAGTGG ATCCATAACT TCGTATAGCA TACATTATAC GAAGTTATCC
      421 CAGATCCCCC GGGCTGCAGC CAATATGGGA TCGGCCATTG AACAAGATGG ATTGCACGCA
      481 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
      541 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC
      601 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG
      661 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG
      721 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT
      781 GCCGANAAAG TATCCATCAT GGCTGANGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
      841 ACCTGCCCAT TCNACCACCA AGCGAANCAT CNNNTCGAGN NNNNNCGTAC TCGGATGGAA
      901 GCCNGNCTTG NCNATCANGA TGNNCTGGNN NAAGANCATC NNNGNCTCGC GCCNGCCNAN
      961 NNNTTCGCNA GGCNCNNNGN NCNCATGCCC NANNNNNANN NNNNCNNCNN NNCCNNNNNN
    1021 NATNNNNGNT NNNCNNNNNN NNNN
    //
    Primer: pAxCALNLF1
    Region: insert 5' (loxP, Arl13b 5')
    Sequence file: RDB16058_A9K8b.seq check
    >D05769A3_A9K8_3_pAxCALNLF1_E02_05_ABI08.ab1
        1 NNNNNNNNNN NNNNNNNNNN NNNCTNNNNN NCCCGATATC CCCATAACTT CGTATAGCAT
       61 ACATTATACG AAGTTATGGA TCCCCCGGGC TGCAGGAATT CCTCGACACG CGTGCTAGCG
      121 CCGCCACCAT GTTCAGTCTG ATGGCCAACT GCTGCAACTT GTTCAAGCGG TGGCGGGAGC
      181 CTGTCAGAAA GGTGACACTT GTGATGGTCG GCCTTGATAA TGCTGGTAAA ACAGCCACAG
      241 CAAAGGGAAT CCAAGGAGAG CATCCTGAAG ACGTAGCTCC CACTGTTGGC TTTTCTAAAA
      301 TTGATCTGAG ACAAGGAAAG TTCCAAGTTA CCATCTTTGA CTTAGGAGGT GGAAAAAGAA
      361 TTCGGGGAAT TTGGAAGAAT TATTATGCTG AATCCTATGG GGTAATATTT GTTGTGGATT
      421 CCAGTGATGA GGAGAGAATG GAAGAAACAA AGGAGACAAT GTCCGAAGTG CTAAGACACC
      481 CGAGGATATC AGGAAAGCCT ATATTGGTGC TGGCCAACAA GCAAGACAAG GAGGGGGCTT
      541 TAGGCGAAGC GGATGTGATT GAGTGTCTCT CGCTGGAGAA GCTGGTCAAC GAGCACAAGT
      601 GCCTGTGTCA GATCGAACCT TGTTCAGCAG TCTTGGGATA TGGAAAGAAA ATTGACAAGT
      661 CCATTAAAAA GGGGCTTTAT TGGCTACTGC ATATCATTGC AAAGGACTTT GATGCCTTAA
      721 GTGAACGCAT CCAGAAAGAC ACAACTGAAC AGCGAGCTCT TGANGAACAA GAGAAACGTG
      781 AGAGGGCTGN NNNNGTCCGG AAGTTAAGGG AAGAANGAGA ACGANAGCAG ACNGAACTTG
      841 ATGNNNCCAG NGGTCTGNNT GANATTGACT CNNNNCAGTT CTTGNGAANN CNTTCNNACC
      901 CATTGCAGCT GTAATCATTG AGAATGAAAA GAAGCNNNAA AAAGANNAAA ANNAANCNGA
      961 NNNNGGANAA NGGNNNNNNG ATGNNGGNCT NNNANNNNAT AAANNNNGNA NCNNGNNNNA
    1021 NCANCGNCNT CNNNNNNNNN GNNNNNANNN NNNNNNN
    //
    Primer: mCerulean-N_R
    Region: insert mid (mCerulean, Arl13b 3')
    Sequence file: RDB16058_A9K8c.seq check
    >D05769A3_A9K8_3_mCerulean-N_R_F02_06_ABI08.ab1
        1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN AGCTNGCCGG NGGTGCAGAT GAACTTCNGG
       61 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG
      121 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
      181 TTGCTCACCA TGGTGGCGAC CGGTGGATCC CCTGAGATCG TGTCCTGAGC ATCACCGTTA
      241 GGTCTCTGTC GCACAGCCAG GGGAGGCAGC GGCTTCCCAT AGAAATCATT GTGACGTGTT
      301 TCACCAAGAG GCTCAAGTTT TGGAAGTCTA GTGACTTTGG GGGTTCCCCA GCCAACAGGA
      361 GGGGGAGGTT GGGGAGGCGT GGGACTCTTT GGAGTAGACT CGTCTGTATT GACTGGTTCT
      421 ACCCGATGAC TCCTTTTCAT TCTTAGTTTC TTGGTTTTCT TCTTACTGTT TTCCCCGGAC
      481 TCTGACCCTC GCTGGTCCTG CTCATCCTCA CTGTCCAGCT GCTGCGACAG TGCCTCCCTG
      541 TAACTGTCCA CCACTCGCTC GTCAGGGTTT TGGAGGCAGC AATCTGCCTC ACTCTGAGGC
      601 GCTGCTTGCT CTGGCTCCAC TTTATGTTCT AGGAGACCAA CATCACTGTC CTTCTCCACG
      661 GTCTGCTTCT TTTTCTCTTT TTCCTGCTTC TTTTCATTCT CAATGATTAC AGCTGCNATG
      721 GGTTGGAAAG GATTCGCAAG AACTGGTCCT GAGTCAATCT CAGCCAGACC ACTGGTCCCA
      781 TCAAGTTCCG TCTGCTCTCG TTCTCTTTCT TCCCTTAACT TCCGGACTCG TTCAGCCCTC
      841 TCACGTTTCT CTTGTTCCTC AAGANCTCGC TGTTCAGTTG TGTCTTTCTG GATGCGTTCA
      901 CTNNANNNAT CAAAGNNCTT TGCAATGATA TGCAGTANCC AATNAAGCCC CCTTTTTANT
    961 GGANTTGTCN ANTTTTCTTT NCNTATNNNA NNNNTGNNNN NNNNNN
    //
    Primer: mVenus-M_F
    Region: insert 3' (mVenus, Gem1_1-110, BGH p(A))
    Sequence file: RDB16058_A9K8d.seq check
    >D05769A3_A9K8_3_mVenus-M_F_C03_09_ABI24.ab1
        1 NNNNNNNNNN NNNNNNNATC NNGGCCNACT TNNNGNTCCG CCNNCAACAT CGAGGNCGGC
       61 GGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
      121 CTGCCCGACA ACCACTACCT GAGCTACCAG TCCAAGCTGA GCAAAGACCC CAACGAGAAG
      181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
      241 GAGCTGTACA AGGGATATCC ATCACACTGG CGGCCGCTCG AGATGAATCC CAGTATGAAG
      301 CAGAAACAAG AAGAAATCAA AGAGAATATA AAGAATAGTT CTGTCCCAAG AAGAACTCTG
      361 AAGATGATTC AGCCTTCTGC ATCTGGATCT CTTGTTGGAA GAGAAAATGA GCTGTCCGCA
      421 GGCTTGTCCA AAAGGAAACA TCGGAATGAC CACTTAACAT CTACAACTTC CAGCCCTGGG
      481 GTTATTGTCC CAGAATCTAG TGAAAATAAA AATCTTGGAG GAGTCACCCA GGAGTCATTT
      541 GATCTTATGA TTAAAGAAAA TCCATCCTCT CAGTATTGGA AGGAAGTGGC AGAAAAACGG
      601 AGAAAGGCGC TGTAAGGTAC CACATGTGGC GCGCGCGCCT TCGATGATCT AGAGCTCGCT
      661 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC
      721 CTTCCTTGAC CCTGGAANGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GANGAAATTG
      781 CATCGCATTG TCTGANTANG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CANGNCAGCA
      841 AGGGGGGAGG ATTGGGAAGA NAATAGCAGG CATGCNGGGG GATGCNGNGG GCNCTATGGC
      901 TTCTGANGCG GAAAGAANCN GCNNCCCNNN CNNGGGCNGN NGGGNNNNNC CNANNNNNNN
    961 NNN
    //
    Primer: P2A_R2
    Region: insert mid (mCerulean)
    Sequence file: RDB16058_A9K8e.seq check
    >D05769A3_A9K8_3_P2A_R2_G06_21_ABI24.ab1
        1 NNNNNNNNNN NNNNNNNNNN NTAGCTCCGC TTCCCTTGTA CNGCTCGTCC NTGCCGAGAG
       61 TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT
      121 CTTTGCTCAG CTTGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT
      181 CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT
      241 TGTGGCGGAT CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA
      301 CGTTGTCGCT GATGGCGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA
      361 AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG
      421 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT
      481 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC
      541 ACTGCACGCC CCAGGTCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG
      601 TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG
      661 ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC
      721 CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGAN CGGTGGATCC CCTGANATCG
      781 TGTCCTGAGC ATCACCGTTA GGTCTCTGTC GCACAGCCAG GGGANGCAGC GGCTTCCCAT
      841 ANAAATCATT GTGACGTGTT TCACCNAGAN GNTCANGTTT TGGAAGTCNA GTGANTTTGG
      901 GGGTTCCCCA GCCNACAGGA NGGGGANGNT GGGGNANGNG NNGGNNTCTT NNGANTANNN
      961 TCNNCNGNAN TGANNNNNNN NACCNNATGA NTCCTTTNCN TTCNNANNTT NNNNNNNNNN
    //
    Primer: FP-C_F1
    Region: insert mid (mCerulean, P2A, mCherry)
    Sequence file: RDB16058_A9K8f.seq check
    >D05769A3_A9K8_3_FP-C_F1_B05_05_ABI24.ab1
        1 NNNNNNNNNN NNNNNNNNNN NCGCGNTCNC NTGGTCCTGC TGGNGTTCGT GNCCGCCGCC
       61 GGGNTCACTC TCGGCATGGA CGAGCTGTAC AAGGGAAGCG GAGCTACTAA CTTCAGCCTG
      121 CTGAAGCAGG CTGGAGACGT GGAGGAGAAC CCTGGACCTG CGCGTATGGT GAGCAAGGGC
      181 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC
      241 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC
      301 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC
      361 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC
      421 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG
      481 GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC
      541 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGANC
      601 ATGGGCTGGG ANGNCTCCTC CGAGCGGATG TACCCCGAGG ANGGCGCCNT GAANGGCGAG
      661 ATCAAGCAGA NGCTGAAGCT GAANGANGGC GGCCACTACG ACGCTGANGT CAAGACCACC
      721 TACNAGGNCN AGAANCCCGT GCAGCTGCCC GGCGCCTACN NCGTCNACNT CAAGTTGGNN
      781 NTNACNTNCC NCNANNAGGA NTNCNCCATC NNNNNNNAGT ACNAANGCGC CNANGGNNGN
    841 NNNTNCNNCN GNGNNNTNNN NNANNNNNNN NNN
    //
    Primer: mCherry-M_F1
    Region: insert mid (mCherry, Cdt1_30-120, T2A, mVenus)
    Sequence file: RDB16058_A9K8g.seq check
    >D05769A3_A9K8_3_mCherry-M_F1_D06_12_ABI24.ab1
        1 NNNNNNNNNN NNNGNNNNNA TGNNGAANAN NNCNTGGGCT GGGANGCCTC CTCCGAGCGG
       61 ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC
      121 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG
      181 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC
      241 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG
      301 TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA GCCCCGCCAG GCCCGCACTC
      361 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC
      421 GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC GGCTGTCGGT GGACGAGGTT
      481 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG
      541 ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC TGACATCCGC GCAGGACCAG
      601 GACACCATCC AATTGGAAGG CCGTGGCTCT CTTCTCACTT GTGGCGATGT CGAAGAGAAT
      661 CCTGGACCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
      721 GAGCTGGACG GCGACGTNNN NNGCCACAAG TTCAGCGTGT CCGGCGAGGG CGANGGCGAT
      781 GCCACCTACG GCAAGCTGAN CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCNCGTGCCC
      841 NGGCCCACCN TCGNGACCAC CCNGGGCTAC NGCCTGCAGN GCTTCGCCCG CTANCCCGAN
      901 CACATGAAGC AGCNCGACTT CTTCNAGNNC GNNNTGNCCN NNNNNTACGT CCANNANCGC
    961 ANNATCNTNN TNNNNNANNA NNGNNNNTAN NNNNCNNNNN NNNNN
    //
    Primer: Cdt1_F1
    Region: insert mid(T2A, mVenus)
    Sequence file: RDB16058_A9K8h.seq check
    >D05769A3_A9K8_3_Cdt1_F1_D05_11_ABI24.ab1
        1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNNCCNTCN NATTGGNAGG CCGTGGCTCT
       61 CTTCTCACTT GTGGCGATGT CGAAGAGAAT CCTGGACCCA TGGTGAGCAA GGGCGAGGAG
      121 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG
      181 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG
      241 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC
      301 GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC
      361 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC
      421 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG
      481 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC
      541 AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG
      601 ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC
      661 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGANCTA CCAGTCCAAG
      721 CTGANCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGANTT CGTGACCGCC
      781 GCCGGGATCA CTCTCGGCAT GGACGANCTG TACNAGGGAT ATCCATCACA CNGGCGGCCG
      841 CTCGAGATGA ATCCCAGTAT GAAGCANAAA CNAGAANAAA TCAAAGAGAA TATAAAGAAT
    901 AGTTCTNNCC NNNNNAGANN NCNGNANNAN GANTNNAGCC TTCNNNNNNN NNN
    //
    Primer: PGKpro_R
    Region: PGK pro, Rosa26 right arm
    Sequence file: RDB16058_A9K8i.seq check
    >D05769A3_A9K8_3_PGKpro_R_H06_24_ABI24.ab1
        1 NNNNNNNNNN NGNNNNNGAN GNGTGCGAGG CCNGANGCCA CTTGTGTAGC GCCNAGTGCC
       61 CAGCGGGGCT GCTAAAGCGC ATGCTCCAGA CTGCCTTGGG AAAAGCGCCT CCCCTACCCG
      121 GTAGAATTCG ATATCAAGCT TATCGATACC GTCGACGGTA TCGATAAGCT AGATGTCCTG
      181 AAATATTTCT ATCAGAACAA GGTAGTATAA AGCTGGTAGG TATACAAAAC GCTAGACTAG
      241 TTTCTATCCC TGACCCTTAA TCTGCTAGTA TATCCGTAGG AAGTTGCTTA AGTGCCACTA
      301 GTACCAACAG CCTCTGTTAG AGTGAACAGT AGATTTTAAT GAAGGGCCAA TAAAGACCAC
      361 TTAAGACTAC TGACTGAATT AAAAATCCAC CCAGTGGAGT TAGTATTGGT TATAGTTATA
      421 TAACAAAATA TCCAACTTAG CCAGGCGTGG TGGTGCACGC CTTTAATCCC GGCACTCAGG
      481 AGGCAGAGGC AGGCGGATTT CTGAGTTCGA GGCCAGCCTG GTCTACAAAG TGAGTTCCAG
      541 GACAGCCAGG GCTATACAGA GAAACCCTGT CTCGAAAAAC CAAAAAAAAA AAAAAAAAGG
      601 AAAAAAAATA TGGCTTGAAA ACATGCTATT GTTGTCAAAA GGAGATCCAA ACAAGTATTT
      661 CTTTGAACAA AAATTATGTA GAAAACAGGG TGATAAAGAA CAAGGGCTTT TTAAAAAAGG
      721 NCAGCATTAT TTGAGATATA CAAGTGACTC CNATTCAAGC CTAAAGGNCA CTCAATGCTC
      781 ACTAACAGTG CTAATCANAA TCAATTCCTT ANCCCAGTCN AAAATAGTCC TGAATATCTT
      841 CTGGANAAAC NTGTCCTCCN ACATATAANC TAGTATACAC TAACAAAANG TCTCNACTTC
      901 AANGNGAAAT GCTTGANTCC TANANTTGNG NNCCANCAAA GNGCTCTATN NNNAGGNNTA
      961 NTNNNTCANA NNNNNTNGNN NGNNANNNNN NNNTANNNNN TNNCNNNNAN NGNNNNNNNN
    1021 GNNAAANNNN NNNNNTTNNN NNNNNNN
    //
    Primer: pUC-M13_-100
    Region: Rosa26 left arm
    Sequence file: RDB16058_A9K8j.seq check
    >D05769A3_A9K8_3_pUC-M13_-100_F05_17_ABI24.ab1
        1 NNNNNNNNNN NNNNNNNNNN NNNGNGNANG CCNGGGTTTT CCCNGTCNCG ACGTTGNNNN
       61 NNGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCACCGC
      121 GGCAGGCCCT CCGAGCGTGG TGGAGCCGTT CTGTGAGACA GCCGGGTACG AGTCGTGACG
      181 CTGGAAGGGG CAAGCGGGTG GTGGGCAGGA ATGCGGTCCG CCCTGCAGCA ACCGGAGGGG
      241 GAGGGAGAAG GGAGCGGAAA AGTCTCCACC GGACGCGGCC ATGGCTCGGG GGGGGGGGGG
      301 CAGNGNANGN NNNCTTCCGG NCNANNTCTC NNCNNTGATT GNNTTCTTTT CCTCCCGCCN
      361 NGNNNGAAAA CACNANTGGN NNGTTTTGNT TGGNGTAAGG CNCCTGNCAN TTAACGGCAN
      421 CNNNANNGNN CANCCGCCGG CNNCCTCNCT CTGCCNNNNG NNNGGGGCGG NAGGNAGGTG
    481 GGGNGAGGNN ANNTGNANNN GCGGGNNCGG TCGNCNNNNG GCGGGGCGGN NNANGNNN
    //
    Primer: PGKpro_F
    Region: DTA, small t intron
    Sequence file: RDB16058_A9K8k.seq check
    >D05769A3_A9K8_3_PGKpro_F_G05_20_ABI24.ab1
        1 NNNNNNNNNN NNNNNNNNNC NNNNNNNCNG GCCTTTCGAN CTGCAGGTCC TCGCCATGGN
       61 TCCTGATGAT GTTGTTGATT CTTCTAAATC TTTTGTGATG GAAAACTTTT CTTCGTACCA
      121 CGGGACTAAA CCTGGTTATG TAGATTCCAT TCAAAAAGGT ATACAAAAGC CAAAATCTGG
      181 TACACAAGGA AATTATGACG ATGATTGGAA AGGGTTTTAT AGTACCGACA ATAAATACGA
      241 CGCTGCGGGA TACTCTGTAG ATAATGAAAA CCCGCTCTCT GGAAAAGCTG GAGGCGTGGT
      301 CAAAGTGACG TATCCAGGAC TGACGAAGGT TCTCGCACTA AAAGTGGATA ATGCCGAAAC
      361 TATTAAGAAA GAGTTAGGTT TAAGTCTCAC TGAACCGTTG ATGGAGCAAG TCGGAACGGA
      421 AGAGTTTATC AAAAGGTTCG GTGATGGTGC TTCGCGTGTA GTGCTCAGCC TTCCCTTCGC
      481 TGAGGGGAGT TCTAGCGTTG AATATATTAA TAACTGGGAA CAGGCGAAAG CGTTAAGCGT
      541 AGAACTTGAG ATTAATTTTG AAACCCGTGG AAAACGTGGC CAAGATGCGA TGTATGAGTA
      601 TATGGCTCAA GCCTGTGCAG GAAATCGTGT CAGGCGATCT CTTTGTGAAG GAACCTTACT
      661 TCTGTGGTGT GACATAATTG GACAAACTAC CTACAGAGAT TTAAAGCTCT AAGGTAAATA
      721 TAAAATTTTT AAGTGTATAA TGTGTTAAAC TACTGATTCT AATTGTTTGT GTATTTTAGA
      781 TTCCNACCTA TGGAACTGAT GAATGGGANC AGNGGTGGAA TGCAGATCCT ANAGCTCGCT
      841 GATCAGCCTC NACTGTGCCT TCTANNTGCC NGCCATCNGN TGTTTGCCCC CNNCCCCCNN
      901 GCCTNNCTTG ANCCTNNAAN GNGNCNCTCC NNNNNNNCNT TNNCTNNNNA ANNNNNNNAN
    961 NTNGNNTNNN NNNNNNN
    //

    Please visit Sequencing and PCR primers for primer information.


    References

    Original, user report and related articles

    original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.
    protocol Van Kerckvoorde M., Live Imaging and Analysis of Cilia and Cell Cycle Dynamics with the Arl13bCerulean-Fucci2a Biosensor and Fucci Tools. Methods Mol. Biol. 2329: 291-309 (2021). PMID 34085231.

    2022.10.12

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