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pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a

Flp/In targeting construct of Arl13bCerulean-Fucci2a

Catalog number RDB16057
Resource name pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a
Clone info. Flp/In targeting construct to make a single insertion into Flp/In compatible cell lines of Arl13bCerulean-Fucci2a a multicistronic version of Fucci2 composed of Arl13bCerulean, mVenus-hGem(1/110) and mCherry-hCdt1(30/120) fused with t2a and p2a.
This clone was used in generating stable cell lines by the depositor.
Vector backbone pCDNA5/Frt (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cells)
Gene/insert name Human CDT1 cDNA Human Gem cDNA Mouse Arl13b cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Jackson, Ian | Mort, Richard |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) | mouse Arl13b (NCBI Gene 68146) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested.
3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
Ordering Please visit Information of Request for Distribution.[link] Please contact us. UCSD's MTA is also required.
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks
Please contact us. UCSD's MTA is also required.
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16057 pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16057_A9K8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F
Region: CAG pro, insert 5' (Arl13b 5')
Sequence file: RDB16057_A9K8a.seq check
>D05769A2_A9K8_2_pCAG-F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNNNNGNN NNNGGCANGG CGGGGTTCGG CTTCTGGCGT
   61 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG
  121 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAC
  181 ACGCGTGCTA GCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTATGTTCA GTCTGATGGC
  241 CAACTGCTGC AACTTGTTCA AGCGGTGGCG GGAGCCTGTC AGAAAGGTGA CACTTGTGAT
  301 GGTCGGCCTT GATAATGCTG GTAAAACAGC CACAGCAAAG GGAATCCAAG GAGAGCATCC
  361 TGAAGACGTA GCTCCCACTG TTGGCTTTTC TAAAATTGAT CTGAGACAAG GAAAGTTCCA
  421 AGTTACCATC TTTGACTTAG GAGGTGGAAA AAGAATTCGG GGAATTTGGA AGAATTATTA
  481 TGCTGAATCC TATGGGGTAA TATTTGTTGT GGATTCCAGT GATGAGGAGA GAATGGAAGA
  541 AACAAAGGAG ACAATGTCCG AAGTGCTAAG ACACCCGAGG ATATCAGGAA AGCCTATATT
  601 GGTGCTGGCC AACAAGCAAG ACAAGGAGGG GGCTTTAGGC GAAGCGGATG TGATTGAGTG
  661 TCTCTCGCTG GAGAAGCTGG TCAACGAGCA CAAGTGCCTG TGTCAGATCG AACCTTGTTC
  721 AGCAGTCTTG GGATATGGAA AGAAAATTGA CAAGTCCATT AAAAAGGGGC TTTATTGGCT
  781 ACTGCATATC ATTGCAAAGG ANTTTGATGC CTTAAGTGAA CGCATCCAGA AAGACACAAC
  841 TGANNAGCGA GCTCTTGANG ANNAAGANNA ANNTGAGAGG GCTGANNGAN NCCGGAAGTT
  901 AAGGGNAGAA AGAGAANGAN ANCAGANGGA ACTTGATGGG ANCNGNGGNC TGGCTGANAN
  961 TGACTCNNNN CNGTTCTNGC NANNCCNTTN NNNNCCNTTG CAGCTGTNNN NNNTGNNNNN
1021 NNAAANNANN NNNAAAAANA NNAAANNNNN NNANNNNNNN NNN
//
Primer: mCerulean-N_R
Region: insert mid (mCerulean, Arl13b 3')
Sequence file: RDB16057_A9K8b.seq check
>D05769A2_A9K8_2_mCerulean-N_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CNNCTNGCCG GNGGTGCNNN NNGAACTTCA
   61 GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC
  121 CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC
  181 CCTTGCTCAC CATGGTGGCG ACCGGTGGAT CCCCTGAGAT CGTGTCCTGA GCATCACCGT
  241 TAGGTCTCTG TCGCACAGCC AGGGGAGGCA GCGGCTTCCC ATAGAAATCA TTGTGACGTG
  301 TTTCACCAAG AGGCTCAAGT TTTGGAAGTC TAGTGACTTT GGGGGTTCCC CAGCCAACAG
  361 GAGGGGGAGG TTGGGGAGGC GTGGGACTCT TTGGAGTAGA CTCGTCTGTA TTGACTGGTT
  421 CTACCCGATG ACTCCTTTTC ATTCTTAGTT TCTTGGTTTT CTTCTTACTG TTTTCCCCGG
  481 ACTCTGACCC TCGCTGGTCC TGCTCATCCT CACTGTCCAG CTGCTGCGAC AGTGCCTCCC
  541 TGTAACTGTC CACCACTCGC TCGTCAGGGT TTTGGAGGCA GCAATCTGCC TCACTCTGAG
  601 GCGCTGCTTG CTCTGGCTCC ACTTTATGTT CTAGGAGACC AACATCACTG TCCTTCTCCN
  661 CGGTCTGCTT CTTTTTCTCT TTTTCCTGCT TCTTTTCATT CTCAATGATT ACAGCTGCAA
  721 TGGGTTGGAA AGGATTCGCA AGAACTGGTC CTGAGTCNAT CTCAGCCAGA CCACTGGTCC
  781 CATCNANGTT CCGTCTGCTC TCGTTCTCTT TCTTCCCTTA ACTTCCGGAC TCGTTCNGCC
  841 CTCTCACGTT TCTCTTGNTC CTNNANGAGC TCGCTGTTCN GTNNTGTCTT TCTGGATGCG
  901 TNCACTTAAN GCATCAAAGN CCTTTGCNNN GATATGCAGT ANCCAATAAN CCNCTTTTTN
  961 NNGNNNTTGT CNNTTTTCTT NNCNTNNNCN NNACTGCTGA NCNNNGTNCN ANNTGANNNN
1021 NNNNNTNNNC TCNTTGANNN NNNNNNN
//
Primer: BGH_rev2
Region: BGH p(A), insert 3' (Gem1_1-110, mVenus)
Sequence file: RDB16057_A9K8c.seq check
>D05769A2_A9K8_2_BGH_rev2_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGCAAANNAC AGNNGGCTGG CAACTAGAAG
   61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCCACT
  121 GTGCTGGATA TCTGCAGAAT TCCACCACAC TGGACTAGTG GATCCGAGCT CGGTACCTTA
  181 CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG GATTTTCTTT
  241 AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT CACTAGATTC
  301 TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC GATGTTTCCT
  361 TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG ATGCAGAAGG
  421 CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT CTTTGATTTC
  481 TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG ATGGATATCC
  541 CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC
  601 CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGTAGC TCAGGTAGTG
  661 GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC
  721 GAGCTGCACG CCGCCGTCCT CGATGTTGTG NCGGATCTTG AAGTTGGCCT TGATGCCGTT
  781 CTTCTGCTTG TCNGCGGTGA TATAGACGTT GTGGCTGTTG TAGTTGTACT CNNGCTTGTG
  841 CCCCNNGANG TTGCCGTCCT NCTTGAANTC NANGCCCTTC NNCTCGATGC NGNTCACCNN
  901 GGNGTCNNCC TCNAACTTCA CCTCNGNNNG GGNNNNGNAG TTGNNNNCNN NCNTGNNNAA
  961 NANGGTGNNC TNNNNGNACG NANCNNTNNG NCNTNGNNGN NNTNANNAAN NTCNNGCTGN
1021 NNNNNNNNN
//
Primer: HygR-M_R
Region: HygR, FRT
Sequence file: RDB16057_A9K8d.seq check
>D05769A2_A9K8_2_HygR-M_R_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNCGA TTCCTTGCGG TCCGNNATGG GCCGAACCCG
   61 CTCGTCTGGC TAAGATCGGC CGCAGCGATC GCATCCATGG CCTCCGCGAC CGGCTGCAGA
  121 ACAGCGGGCA GTTCGGTTTC AGGCAGGTCT TGCAACGTGA CACCCTGTGC ACGGCGGGAG
  181 ATGCAATAGG TCAGGCTCTC GCTGAATTCC CCAATGTCAA GCACTTCCGG AATCGGGAGC
  241 GCGGCCGATG CAAAGTGCCG ATAAACATAA CGATCTTTGT AGAAACCATC GGCGCAGCTA
  301 TTTACCCGCA GGACATATCC ACGCCCTCCT ACATCGAAGC TGAAAGCACG AGATTCTTCG
  361 CCCTCCGAGA GCTGCATCAG GTCGGAGACG CTGTCGAACT TTTCGATCAG AAACTTCTCG
  421 ACAGACGTCG CGGTGAGTTC AGGCTTTTTG GCCAAGGAAG TTCCTATACT TTCTAGAGAA
  481 TAGGAACTTC GGAATAGGAA CTTCTAGGTA CGTGAACCAT CACCCTAATC AAGTTTTTTG
  541 GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT
  601 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC
  661 GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT
  721 AATGCGCCGC TACAGGGCGC GTGGGGATAC CCCCTAGAGC CCCAGCTGGT TCTTTCCNCC
  781 TCANAAGCCA TAGAGCCCAC CGCATCCCCN GCATGCCTGC TATTGTCTTC CCAATCCTCC
  841 CCCTTGCTGT CCTGCCCCAC CCCACCCCCC NGAANNAGAA TGACNCCTAC TCNNANAATG
  901 CNATGCAATT NNNTCATTTT ATTANGAAAG GACNGNGGGA GTGNCACCTN NCANGGNCNN
  961 NGNAANGNNN GGGGGANGGN NAAACNACNN ANNGNTNGNN ACTANAANNN NNCNNNNNAN
1021 GNNTNNNCNN NNGNNTTNAA NNNGNNNNNN NNNN
//
Primer: P2A_R1
Region: insert mid (mCerulean)
Sequence file: RDB16057_A9K8e.seq check
>D05769A2_A9K8_2_P2A_R1_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG NNCCCGGCGG CGGTCACGAA CTCCNGCAGG
   61 ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT TGGACTGGGT GCTCAGGTAG
  121 TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG
  181 GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTGGC CTTGATGCCG
  241 TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTCGCTGA TGGCGTTGTA CTCCAGCTTG
  301 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC
  361 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG
  421 AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC
  481 TTCATGTGGT CGGGGTAGCG GGCGAAGCAC TGCACGCCCC AGGTCAGGGT GGTCACGAGG
  541 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG
  601 TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
  661 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
  721 GTGGCGACCG GTGGATCCCC TGANATCGTG TCCTGANCAT CACCGTTAGG TCTCTGTCGC
  781 ACAGCCNGGG GANGCANCGN CTTCCCATAG AAATCATTGT GACNTGTTTC ACCNAGANGC
  841 TCNAGTTTTG GAAGTCTANT GACTTNNGGG GNNNCCCANC CNACAGGNNG GGGNNGTNGG
  901 GGNNGCGTGG GANTCTTTGN ANTANANTCN NCTGTATTGA CTGGNTCTAC CCNATNNNTC
961 CNTTTNCNTT CTTANTTTCN TGGNTTTNCN NNNNNNNNN
//
Primer: mCherry-C_R2
Region: insert mid (mCherry, P2A, mCerulean)
Sequence file: RDB16057_A9K8f.seq check
>D05769A2_A9K8_2_mCherry-C_R2_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT TNNGCTTNNG CCTCTGCTTG NTCTCGCCCT TCAGGGCGCC
   61 GTCCTCGGGG TACATCCGCT CGGAGGAGGC CTCCCAGCCC ATGGTCTTCT TCTGCATTAC
  121 GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC TTGTAGATGA ACTCGCCGTC
  181 CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG TCCTCGAAGT TCATCACGCG
  241 CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG TCGGGGATGT CGGCGGGGTG
  301 CTTCACGTAG GCCTTGGAGC CGTACATGAA CTGAGGGGAC AGGATGTCCC AGGCGAAGGG
  361 CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC
  421 CTCGCCCTCG CCCTCGATCT CGAACTCGTG GCCGTTCACG GAGCCCTCCA TGTGCACCTT
  481 GAAGCGCATG AACTCCTTGA TGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATACG
  541 CGCAGGTCCA GGGTTCTCCT CCACGTCTCC AGCCTGCTTC AGCAGGCTGA AGTTAGTAGC
  601 TCCGCTTCCC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC
  661 CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGGGTGCT
  721 CAGGTAGTGN TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA
  781 GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTGGCCTT
  841 NATGCCGTTC TTCTGCTTGT CGGCGGTGAT ATAGACGTTG TCGCTGATGG CGTTGTANTC
  901 CAGCTNGTGN CCCANGATGT TGCCGNCCTC CTTGAAGNNN ANGCCCTNCA NCTCGATGCN
961 GNTTCACCAN GGNNNNGCCC NCNAACTTCN NCNNNNNCGC GGGNNTNNNN NNNNNN
//
Primer: mCherry-M_F1
Region: insert mid (mCherry, Cdt_30-120, T2A, mVenus)
Sequence file: RDB16057_A9K8g.seq check
>D05769A2_A9K8_2_mCherry-M_F1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNNNATGNN GNNNNNNCNT GGGCTGGGAN GCCTCCTCCG
   61 AGCGGATGTA CCCCGAGGNC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA
  121 AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC
  181 AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT
  241 ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG
  301 AGCTGTACAA GGGATATCCA TCACACTGGC GGCCGCTCGA GCCCAGCCCC GCCAGGCCCG
  361 CACTCCGCGC CCCGGCCTCC GCTACCAGTG GCAGCCGCAA GCGCGCCCGC CCGCCCGCCG
  421 CCCCCGGACG CGACCAGGCC AGGCCACCGG CCCGCAGGAG ACTGCGGCTG TCGGTGGACG
  481 AGGTTTCCAG CCCCAGTACC CCCGAGGCCC CAGACATCCC AGCCTGCCCT TCTCCGGGCC
  541 AGAAGATAAA GAAATCCACC CCGGCAGCAG GTCAGCCGCC CCACCTGACA TCCGCGCAGG
  601 ACCAGGACAC CATCCAATTG GAAGGCCGTG GCTCTCTTCT CACTTGTGGC GATGTCGAAG
  661 AGAATCCTGG ACCCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC
  721 TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG
  781 GCGATGCCNC CTACGGCAAG CTGANCCTGA AGCTGATCTG CACCACCNGC AAGCTGCCCG
  841 TGCCCTGNCC CACCCTCGTG ANCACCCTGG GCTACNGNCT GCAGTGCTTC NCCCGCTACC
  901 CCGACCNCAT GANNCAGCAC GACNNCTTCN AGTCCGCCNN GNCNNNNNNC TNNGTCCAGG
961 ANCGCACCNN NTNNTNNNNN GNNNNNNGNN NNNNNNNANN NNNNNCNNNN NNNNNN
//
Primer: Cdt1_F1
Region: insert mid (T2A, mVenus, Gem1_1-110)
Sequence file: RDB16057_A9K8h.seq check
>D05769A2_A9K8_2_Cdt1_F1_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN CGNNNGNNCN GGNNNCCATC NNATTGGAAG GNCGTGGCTC
   61 TCTTCTCACT TGTGGCGATG TCGAAGAGAA TCCTGGACCC ATGGTGAGCA AGGGCGAGGA
  121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
  181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT
  241 GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA
  301 CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
  361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
  421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
  481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
  541 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA
  601 GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
  661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCAA
  721 GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
  781 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC
  841 GCTCGAGATG AATCCCAGTA TGAAGCAGAA ACAAGAAGAA ATCAAANGAG AATATAAAGA
  901 ATAGTTCTGN CCCNAGAAGA ACTCTGAAGA NGANNCAGCN NTCTGCATCT GGNTNTCTNN
961 NNGGNNGANA AAATGANCTN NNNNNNNNTN NNCCNAAANN NAANNNTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl