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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a

Flp/In targeting construct of Arl13bCerulean-Fucci2a

Catalog number RDB16057
Resource name pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a
Clone info. Flp/In targeting construct to make a single insertion into Flp/In compatible cell lines of Arl13bCerulean-Fucci2a a multicistronic version of Fucci2 composed of Arl13bCerulean, mVenus-hGem(1/110) and mCherry-hCdt1(30/120) fused with t2a and p2a.
Comment This clone was used in generating stable cell lines by the depositor.
Vector backbone pCDNA5/Frt (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cells)
Gene/insert name Human CDT1 cDNA Human Gem cDNA Mouse Arl13b cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Jackson, Ian | Mort, Richard |
Other clones in our bank

External Database
Human CDT1 Human Gem Mouse Arl13b Discosoma sp. RFP
    Aequorea victoria GFP

      Reference sequence
        
       

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions for distribution 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
      2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested.
      3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
      4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
      5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
      6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
      Additional terms and conditions:
      RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
      Ordering Please visit Information of Request for Distribution.[link] 
      Order form 
      [Credit Card Payment]  [Bank Transfer Payment]
      Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
      This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
      Approval form [Word] by Dr. Atsushi Miyawaki
      Remarks
      This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
      Please obtain written approval of Dr. Atsushi Miyawaki.
      提供案内 (日本国内) [open/close]

      提供条件 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans.
      2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018)) is requested.
      3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested.
      4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
      5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT.
      6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties.
      付加的提供条件:
      利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
      提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
      提供依頼書 [Word]
      提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
      GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
      宮脇敦史博士による提供承諾書 [Word]
      備考 提供条件の言語は英語のみ。
      Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
      宮脇敦史博士の書面による提供承諾が必要です。

      Catalog # Resource name Shipping form Fee (non-profit org.)
      RDB16057 pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a DNA solution

      Please review the QC test results indicated by check icon below as well as clone information before placing your order.

      References and tips

      Original reference

      original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.

      Further references such as user reports and related articles (go to bottom)

      Featured content

      Featured content Fucci-related materials (en) (English text)
      Featured content Fucci-related materials (ja) (Japanese text)

      QC test results

      RIKEN BRC has sequenced portions of this material for quality test.
      Please review the QC test results indicated by check icon as well as clone information before placing your order.

      Test sheet RDB16057_A9K8p1-2.pdf check

      Nucleotide sequence of a portion of this resource (if available).

      Primer: pCAG-F
      Region: CAG pro, insert 5' (Arl13b 5')
      Sequence file: RDB16057_A9K8a.seq check
      >D05769A2_A9K8_2_pCAG-F_C06_09_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NTNNNNNGNN NNNGGCANGG CGGGGTTCGG CTTCTGGCGT
         61 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG
        121 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAC
        181 ACGCGTGCTA GCGCTACCGG ACTCAGATCT CGAGCTCAAG CTTATGTTCA GTCTGATGGC
        241 CAACTGCTGC AACTTGTTCA AGCGGTGGCG GGAGCCTGTC AGAAAGGTGA CACTTGTGAT
        301 GGTCGGCCTT GATAATGCTG GTAAAACAGC CACAGCAAAG GGAATCCAAG GAGAGCATCC
        361 TGAAGACGTA GCTCCCACTG TTGGCTTTTC TAAAATTGAT CTGAGACAAG GAAAGTTCCA
        421 AGTTACCATC TTTGACTTAG GAGGTGGAAA AAGAATTCGG GGAATTTGGA AGAATTATTA
        481 TGCTGAATCC TATGGGGTAA TATTTGTTGT GGATTCCAGT GATGAGGAGA GAATGGAAGA
        541 AACAAAGGAG ACAATGTCCG AAGTGCTAAG ACACCCGAGG ATATCAGGAA AGCCTATATT
        601 GGTGCTGGCC AACAAGCAAG ACAAGGAGGG GGCTTTAGGC GAAGCGGATG TGATTGAGTG
        661 TCTCTCGCTG GAGAAGCTGG TCAACGAGCA CAAGTGCCTG TGTCAGATCG AACCTTGTTC
        721 AGCAGTCTTG GGATATGGAA AGAAAATTGA CAAGTCCATT AAAAAGGGGC TTTATTGGCT
        781 ACTGCATATC ATTGCAAAGG ANTTTGATGC CTTAAGTGAA CGCATCCAGA AAGACACAAC
        841 TGANNAGCGA GCTCTTGANG ANNAAGANNA ANNTGAGAGG GCTGANNGAN NCCGGAAGTT
        901 AAGGGNAGAA AGAGAANGAN ANCAGANGGA ACTTGATGGG ANCNGNGGNC TGGCTGANAN
        961 TGACTCNNNN CNGTTCTNGC NANNCCNTTN NNNNCCNTTG CAGCTGTNNN NNNTGNNNNN
      1021 NNAAANNANN NNNAAAAANA NNAAANNNNN NNANNNNNNN NNN
      //
      Primer: mCerulean-N_R
      Region: insert mid (mCerulean, Arl13b 3')
      Sequence file: RDB16057_A9K8b.seq check
      >D05769A2_A9K8_2_mCerulean-N_R_B01_02_ABI08.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CNNCTNGCCG GNGGTGCNNN NNGAACTTCA
         61 GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC
        121 CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC
        181 CCTTGCTCAC CATGGTGGCG ACCGGTGGAT CCCCTGAGAT CGTGTCCTGA GCATCACCGT
        241 TAGGTCTCTG TCGCACAGCC AGGGGAGGCA GCGGCTTCCC ATAGAAATCA TTGTGACGTG
        301 TTTCACCAAG AGGCTCAAGT TTTGGAAGTC TAGTGACTTT GGGGGTTCCC CAGCCAACAG
        361 GAGGGGGAGG TTGGGGAGGC GTGGGACTCT TTGGAGTAGA CTCGTCTGTA TTGACTGGTT
        421 CTACCCGATG ACTCCTTTTC ATTCTTAGTT TCTTGGTTTT CTTCTTACTG TTTTCCCCGG
        481 ACTCTGACCC TCGCTGGTCC TGCTCATCCT CACTGTCCAG CTGCTGCGAC AGTGCCTCCC
        541 TGTAACTGTC CACCACTCGC TCGTCAGGGT TTTGGAGGCA GCAATCTGCC TCACTCTGAG
        601 GCGCTGCTTG CTCTGGCTCC ACTTTATGTT CTAGGAGACC AACATCACTG TCCTTCTCCN
        661 CGGTCTGCTT CTTTTTCTCT TTTTCCTGCT TCTTTTCATT CTCAATGATT ACAGCTGCAA
        721 TGGGTTGGAA AGGATTCGCA AGAACTGGTC CTGAGTCNAT CTCAGCCAGA CCACTGGTCC
        781 CATCNANGTT CCGTCTGCTC TCGTTCTCTT TCTTCCCTTA ACTTCCGGAC TCGTTCNGCC
        841 CTCTCACGTT TCTCTTGNTC CTNNANGAGC TCGCTGTTCN GTNNTGTCTT TCTGGATGCG
        901 TNCACTTAAN GCATCAAAGN CCTTTGCNNN GATATGCAGT ANCCAATAAN CCNCTTTTTN
        961 NNGNNNTTGT CNNTTTTCTT NNCNTNNNCN NNACTGCTGA NCNNNGTNCN ANNTGANNNN
      1021 NNNNNTNNNC TCNTTGANNN NNNNNNN
      //
      Primer: BGH_rev2
      Region: BGH p(A), insert 3' (Gem1_1-110, mVenus)
      Sequence file: RDB16057_A9K8c.seq check
      >D05769A2_A9K8_2_BGH_rev2_C01_03_ABI08.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGCAAANNAC AGNNGGCTGG CAACTAGAAG
         61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCCACT
        121 GTGCTGGATA TCTGCAGAAT TCCACCACAC TGGACTAGTG GATCCGAGCT CGGTACCTTA
        181 CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG GATTTTCTTT
        241 AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT CACTAGATTC
        301 TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC GATGTTTCCT
        361 TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG ATGCAGAAGG
        421 CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT CTTTGATTTC
        481 TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG ATGGATATCC
        541 CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC
        601 CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGTAGC TCAGGTAGTG
        661 GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC
        721 GAGCTGCACG CCGCCGTCCT CGATGTTGTG NCGGATCTTG AAGTTGGCCT TGATGCCGTT
        781 CTTCTGCTTG TCNGCGGTGA TATAGACGTT GTGGCTGTTG TAGTTGTACT CNNGCTTGTG
        841 CCCCNNGANG TTGCCGTCCT NCTTGAANTC NANGCCCTTC NNCTCGATGC NGNTCACCNN
        901 GGNGTCNNCC TCNAACTTCA CCTCNGNNNG GGNNNNGNAG TTGNNNNCNN NCNTGNNNAA
        961 NANGGTGNNC TNNNNGNACG NANCNNTNNG NCNTNGNNGN NNTNANNAAN NTCNNGCTGN
      1021 NNNNNNNNN
      //
      Primer: HygR-M_R
      Region: HygR, FRT
      Sequence file: RDB16057_A9K8d.seq check
      >D05769A2_A9K8_2_HygR-M_R_D01_04_ABI08.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNGNNCGA TTCCTTGCGG TCCGNNATGG GCCGAACCCG
         61 CTCGTCTGGC TAAGATCGGC CGCAGCGATC GCATCCATGG CCTCCGCGAC CGGCTGCAGA
        121 ACAGCGGGCA GTTCGGTTTC AGGCAGGTCT TGCAACGTGA CACCCTGTGC ACGGCGGGAG
        181 ATGCAATAGG TCAGGCTCTC GCTGAATTCC CCAATGTCAA GCACTTCCGG AATCGGGAGC
        241 GCGGCCGATG CAAAGTGCCG ATAAACATAA CGATCTTTGT AGAAACCATC GGCGCAGCTA
        301 TTTACCCGCA GGACATATCC ACGCCCTCCT ACATCGAAGC TGAAAGCACG AGATTCTTCG
        361 CCCTCCGAGA GCTGCATCAG GTCGGAGACG CTGTCGAACT TTTCGATCAG AAACTTCTCG
        421 ACAGACGTCG CGGTGAGTTC AGGCTTTTTG GCCAAGGAAG TTCCTATACT TTCTAGAGAA
        481 TAGGAACTTC GGAATAGGAA CTTCTAGGTA CGTGAACCAT CACCCTAATC AAGTTTTTTG
        541 GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT
        601 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC
        661 GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT
        721 AATGCGCCGC TACAGGGCGC GTGGGGATAC CCCCTAGAGC CCCAGCTGGT TCTTTCCNCC
        781 TCANAAGCCA TAGAGCCCAC CGCATCCCCN GCATGCCTGC TATTGTCTTC CCAATCCTCC
        841 CCCTTGCTGT CCTGCCCCAC CCCACCCCCC NGAANNAGAA TGACNCCTAC TCNNANAATG
        901 CNATGCAATT NNNTCATTTT ATTANGAAAG GACNGNGGGA GTGNCACCTN NCANGGNCNN
        961 NGNAANGNNN GGGGGANGGN NAAACNACNN ANNGNTNGNN ACTANAANNN NNCNNNNNAN
      1021 GNNTNNNCNN NNGNNTTNAA NNNGNNNNNN NNNN
      //
      Primer: P2A_R1
      Region: insert mid (mCerulean)
      Sequence file: RDB16057_A9K8e.seq check
      >D05769A2_A9K8_2_P2A_R1_E01_05_ABI08.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG NNCCCGGCGG CGGTCACGAA CTCCNGCAGG
         61 ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT TGGACTGGGT GCTCAGGTAG
        121 TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG
        181 GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTGGC CTTGATGCCG
        241 TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTCGCTGA TGGCGTTGTA CTCCAGCTTG
        301 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC
        361 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG
        421 AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC
        481 TTCATGTGGT CGGGGTAGCG GGCGAAGCAC TGCACGCCCC AGGTCAGGGT GGTCACGAGG
        541 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG
        601 TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
        661 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
        721 GTGGCGACCG GTGGATCCCC TGANATCGTG TCCTGANCAT CACCGTTAGG TCTCTGTCGC
        781 ACAGCCNGGG GANGCANCGN CTTCCCATAG AAATCATTGT GACNTGTTTC ACCNAGANGC
        841 TCNAGTTTTG GAAGTCTANT GACTTNNGGG GNNNCCCANC CNACAGGNNG GGGNNGTNGG
        901 GGNNGCGTGG GANTCTTTGN ANTANANTCN NCTGTATTGA CTGGNTCTAC CCNATNNNTC
      961 CNTTTNCNTT CTTANTTTCN TGGNTTTNCN NNNNNNNNN
      //
      Primer: mCherry-C_R2
      Region: insert mid (mCherry, P2A, mCerulean)
      Sequence file: RDB16057_A9K8f.seq check
      >D05769A2_A9K8_2_mCherry-C_R2_B06_06_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNT TNNGCTTNNG CCTCTGCTTG NTCTCGCCCT TCAGGGCGCC
         61 GTCCTCGGGG TACATCCGCT CGGAGGAGGC CTCCCAGCCC ATGGTCTTCT TCTGCATTAC
        121 GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC TTGTAGATGA ACTCGCCGTC
        181 CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG TCCTCGAAGT TCATCACGCG
        241 CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG TCGGGGATGT CGGCGGGGTG
        301 CTTCACGTAG GCCTTGGAGC CGTACATGAA CTGAGGGGAC AGGATGTCCC AGGCGAAGGG
        361 CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC
        421 CTCGCCCTCG CCCTCGATCT CGAACTCGTG GCCGTTCACG GAGCCCTCCA TGTGCACCTT
        481 GAAGCGCATG AACTCCTTGA TGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATACG
        541 CGCAGGTCCA GGGTTCTCCT CCACGTCTCC AGCCTGCTTC AGCAGGCTGA AGTTAGTAGC
        601 TCCGCTTCCC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC
        661 CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGGGTGCT
        721 CAGGTAGTGN TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA
        781 GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTGGCCTT
        841 NATGCCGTTC TTCTGCTTGT CGGCGGTGAT ATAGACGTTG TCGCTGATGG CGTTGTANTC
        901 CAGCTNGTGN CCCANGATGT TGCCGNCCTC CTTGAAGNNN ANGCCCTNCA NCTCGATGCN
      961 GNTTCACCAN GGNNNNGCCC NCNAACTTCN NCNNNNNCGC GGGNNTNNNN NNNNNN
      //
      Primer: mCherry-M_F1
      Region: insert mid (mCherry, Cdt_30-120, T2A, mVenus)
      Sequence file: RDB16057_A9K8g.seq check
      >D05769A2_A9K8_2_mCherry-M_F1_G01_07_ABI08.ab1
          1 NNNNNNNNNN NNNNNNNNNN CNNNNATGNN GNNNNNNCNT GGGCTGGGAN GCCTCCTCCG
         61 AGCGGATGTA CCCCGAGGNC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA
        121 AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC
        181 AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT
        241 ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG
        301 AGCTGTACAA GGGATATCCA TCACACTGGC GGCCGCTCGA GCCCAGCCCC GCCAGGCCCG
        361 CACTCCGCGC CCCGGCCTCC GCTACCAGTG GCAGCCGCAA GCGCGCCCGC CCGCCCGCCG
        421 CCCCCGGACG CGACCAGGCC AGGCCACCGG CCCGCAGGAG ACTGCGGCTG TCGGTGGACG
        481 AGGTTTCCAG CCCCAGTACC CCCGAGGCCC CAGACATCCC AGCCTGCCCT TCTCCGGGCC
        541 AGAAGATAAA GAAATCCACC CCGGCAGCAG GTCAGCCGCC CCACCTGACA TCCGCGCAGG
        601 ACCAGGACAC CATCCAATTG GAAGGCCGTG GCTCTCTTCT CACTTGTGGC GATGTCGAAG
        661 AGAATCCTGG ACCCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC
        721 TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG
        781 GCGATGCCNC CTACGGCAAG CTGANCCTGA AGCTGATCTG CACCACCNGC AAGCTGCCCG
        841 TGCCCTGNCC CACCCTCGTG ANCACCCTGG GCTACNGNCT GCAGTGCTTC NCCCGCTACC
        901 CCGACCNCAT GANNCAGCAC GACNNCTTCN AGTCCGCCNN GNCNNNNNNC TNNGTCCAGG
      961 ANCGCACCNN NTNNTNNNNN GNNNNNNGNN NNNNNNNANN NNNNNCNNNN NNNNNN
      //
      Primer: Cdt1_F1
      Region: insert mid (T2A, mVenus, Gem1_1-110)
      Sequence file: RDB16057_A9K8h.seq check
      >D05769A2_A9K8_2_Cdt1_F1_B02_02_ABI08.ab1
          1 NNNNNNNNNN NNNNNNNNNN CGNNNGNNCN GGNNNCCATC NNATTGGAAG GNCGTGGCTC
         61 TCTTCTCACT TGTGGCGATG TCGAAGAGAA TCCTGGACCC ATGGTGAGCA AGGGCGAGGA
        121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
        181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT
        241 GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA
        301 CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
        361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
        421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
        481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
        541 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA
        601 GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
        661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCAA
        721 GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
        781 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC
        841 GCTCGAGATG AATCCCAGTA TGAAGCAGAA ACAAGAAGAA ATCAAANGAG AATATAAAGA
        901 ATAGTTCTGN CCCNAGAAGA ACTCTGAAGA NGANNCAGCN NTCTGCATCT GGNTNTCTNN
      961 NNGGNNGANA AAATGANCTN NNNNNNNNTN NNCCNAAANN NAANNNTNNN NNNNNNN
      //

      Please visit Sequencing and PCR primers for primer information.


      References

      Original, user report and related articles

      original Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.
      protocol Van Kerckvoorde M., Live Imaging and Analysis of Cilia and Cell Cycle Dynamics with the Arl13bCerulean-Fucci2a Biosensor and Fucci Tools. Methods Mol. Biol. 2329: 291-309 (2021). PMID 34085231.

      2022.10.12

      GNP_filter3_RDBDEP_html_221010.pl