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Sec63N-V5-GFP11

Expression vector of human SEC63 homolog, protein translocation regulator (Sec63), N-terminal segments 1-240 for visualizing organelle contact sites in vivo.

Catalog number RDB16048
Resource name Sec63N-V5-GFP11
Alternative name pcDNA3.1_Sec63_GFP11 (pMM89)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human SEC63 homolog, protein translocation regulator (Sec63), N-terminal segments 1-240 fused with V5-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human SEC63 cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
human SEC63

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16048 Sec63N-V5-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16048_A8I3p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB16048_A8I3a.seq check
          >D05803_B3_A8I3_1_CMV-Forward_D09_12_ABI24.ab1
              1 CTATCACCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG
            181 GATCCGCCAC CATGGCCGGA CAACAGTTCC AGTATGACGA CTCAGGGAAC ACCTTCTTCT
            241 ACTTCCTGAC ATCCTTCGTC GGCCTGATCG TCATACCAGC GACCTACTAC CTCTGGCCCA
            301 GAGATCAGAA CGCAGAACAG ATACGGCTCA AGAACATTCG TAAGGTGTAT GGACGGTGCA
            361 TGTGGTACCG CCTTAGACTG CTGAAACCTC AACCCAACAT CATACCCACT GTCAAGAAAA
            421 TCGTGCTGTT GGCAGGATGG GCTCTGTTTC TCTTTCTGGC CTACAAAGTG AGCAAGACAG
            481 ACAGGGAGTA TCAGGAGTAC AACCCGTATG AAGTGCTCAA TCTTGATCCA GGCGCTACAG
            541 TGGCCGAAAT CAAGAAGCAG TATCGATTGT TGAGCCTCAA GTATCACCCC GATAAAGGAG
            601 GCGATGAGGT GATGTTTATG CGCATTGCCA AAGCGTATGC AGCACTGACT GACGAAGAGT
            661 CTAGGAAGAA TTGGGAGGAG TTTGGGAATC CTGATGGTCC ACAAGCTACC TCCTTTGGTA
            721 TCGCCCTTCC TGCTTGGATC GTTGACCAGA AGAACAGTAT TCTGGTCCTG CTGGTTTACG
            781 GACTTGCCTT TATGGTGATT CTGCCAGTAG TAGTTGGGTC ATGGTGGTAT AGGTCCATCA
            841 GATACAGCGG GGACCAGATT CTGATTCGGA CGACACAGAT TTACACCTAC TTCGTGTACA
            901 AAACCCGCAA TGGCAAACCT ATCCCGAATC CCCTGTTGGG GCTGGGATAG TACTGCGGGT
            961 TCTGGGCGGC GCCACCGCGG ATGTAGGTCT GGTGCCGCAT CCACCAGTTC GTTGAACACA
          1021 TGGATCGATC ATGAGTTACG GTAATGCTGC TGGATCACAT ATCCTAGAGG GATC
          //
          Primer: BGH_rev2
          Sequence file: RDB16048_A8I3b.seq check
          >D05803_B3_A8I3_1_BGH_rev2_E09_15_ABI24.ab1
              1 TTCATGTCAG AGCACGGGGG AGGGGCAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
             61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGATTATG TAATCCCAGC AGCATTTACG
            121 TACTCATGAA GGACCATGTG GTCACGACTT GTTGATCCGC CACCAGACCC TCCATCGGCG
            181 GCCGCGCCGC CAGAACCGCC AGTACTATCC AGCCCCAACA GGGGATTCGG GATAGGTTTG
            241 CCATTGCGGG TTTTGTACAC GAAGTAGGTG TAAATCTGTG TCGTCCGAAT CAGAATCTGG
            301 TCCCCGCTGT ATCTGATGGA CCTATACCAC CATGACCCAA CTACTACTGG CAGAATCACC
            361 ATAAAGGCAA GTCCGTAAAC CAGCAGGACC AGAATACTGT TCTTCTGGTC AACGATCCAA
            421 GCAGGAAGGG CGATACCAAA GGAGGTAGCT TGTGGACCAT CAGGATTCCC AAACTCCTCC
            481 CAATTCTTCC TAGACTCTTC GTCAGTCAGT GCTGCATACG CTTTGGCAAT GCGCATAAAC
            541 ATCACCTCAT CGCCTCCTTT ATCGGGGTGA TACTTGAGGC TCAACAATCG ATACTGCTTC
            601 TTGATTTCGG CCACTGTAGC GCCTGGATCA AGATTGAGCA CTTCATACGG GTTGTACTCC
            661 TGATACTCCC TGTCTGTCTT GCTCACTTTG TAGGCCAGAA AGAGAAACAG AGCCCATCCT
            721 GCCAACAGCA CGATTTTCTT GACAGTGGGT ATGATGTTGG GGTTGAGGTT TCAGCAGTCT
            781 AAGGCGGTAC CACATGCACC GTCCATACAC CTTACGAATG TTCTTGAGCC GTATCTGTTC
            841 TGCGTTCTGA TCTCTGGGCC AGAGGTAGTA GGTCGCTGGT ATGACGATCA GGCCGACGAA
            901 GGATGTCAGA AGGTAGAGAA TGTGTTTCCC TGAGTCGTCA TACTGGACTG TGTCGGCATG
            961 GTGGCGGATC GAGCTCGTAT AGCTTAGTTA ACGCTAGCCA GCTGGGTCTC CCTATAGTGA
          1021 GTCGTATTAA TCGATAGCCA GTTAGCATTG GTTCTCTAGT G
          //
          Primer: SV40pro_F
          Sequence file: RDB16048_A8I3c.seq check
          >D05803_B3_A8I3_1_SV40pro_F_F09_18_ABI24.ab1
              1 CCCGGGTCCT GGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
            121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
            661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
            961 CGCCCAACCC TGCCATCACG AGATTTCGAT TCCCACCCGC CCGTCTTCTA TGAAGTTTGG
           1021 GCTTCGGCAA TCGGTTTTCC CGGCACGACT GGACTGCAAT GAATCTTCCA GCCGCCGGGG
          1081 GATTCTCCAT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl