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Sec63N-V5-GFP11

Expression vector of human SEC63 homolog, protein translocation regulator (Sec63), N-terminal segments 1-240 for visualizing organelle contact sites in vivo.

Catalog number RDB16048
Resource name Sec63N-V5-GFP11
Alternative name pcDNA3.1_Sec63_GFP11 (pMM89)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human SEC63 homolog, protein translocation regulator (Sec63), N-terminal segments 1-240 fused with V5-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human SEC63 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank human SEC63 (NCBI Gene 11231) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
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RDB16048 Sec63N-V5-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16048_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB16048_A8I3a.seq check
>D05803_B3_A8I3_1_CMV-Forward_D09_12_ABI24.ab1
    1 CTATCACCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG
  181 GATCCGCCAC CATGGCCGGA CAACAGTTCC AGTATGACGA CTCAGGGAAC ACCTTCTTCT
  241 ACTTCCTGAC ATCCTTCGTC GGCCTGATCG TCATACCAGC GACCTACTAC CTCTGGCCCA
  301 GAGATCAGAA CGCAGAACAG ATACGGCTCA AGAACATTCG TAAGGTGTAT GGACGGTGCA
  361 TGTGGTACCG CCTTAGACTG CTGAAACCTC AACCCAACAT CATACCCACT GTCAAGAAAA
  421 TCGTGCTGTT GGCAGGATGG GCTCTGTTTC TCTTTCTGGC CTACAAAGTG AGCAAGACAG
  481 ACAGGGAGTA TCAGGAGTAC AACCCGTATG AAGTGCTCAA TCTTGATCCA GGCGCTACAG
  541 TGGCCGAAAT CAAGAAGCAG TATCGATTGT TGAGCCTCAA GTATCACCCC GATAAAGGAG
  601 GCGATGAGGT GATGTTTATG CGCATTGCCA AAGCGTATGC AGCACTGACT GACGAAGAGT
  661 CTAGGAAGAA TTGGGAGGAG TTTGGGAATC CTGATGGTCC ACAAGCTACC TCCTTTGGTA
  721 TCGCCCTTCC TGCTTGGATC GTTGACCAGA AGAACAGTAT TCTGGTCCTG CTGGTTTACG
  781 GACTTGCCTT TATGGTGATT CTGCCAGTAG TAGTTGGGTC ATGGTGGTAT AGGTCCATCA
  841 GATACAGCGG GGACCAGATT CTGATTCGGA CGACACAGAT TTACACCTAC TTCGTGTACA
  901 AAACCCGCAA TGGCAAACCT ATCCCGAATC CCCTGTTGGG GCTGGGATAG TACTGCGGGT
  961 TCTGGGCGGC GCCACCGCGG ATGTAGGTCT GGTGCCGCAT CCACCAGTTC GTTGAACACA
1021 TGGATCGATC ATGAGTTACG GTAATGCTGC TGGATCACAT ATCCTAGAGG GATC
//
Primer: BGH_rev2
Sequence file: RDB16048_A8I3b.seq check
>D05803_B3_A8I3_1_BGH_rev2_E09_15_ABI24.ab1
    1 TTCATGTCAG AGCACGGGGG AGGGGCAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGATTATG TAATCCCAGC AGCATTTACG
  121 TACTCATGAA GGACCATGTG GTCACGACTT GTTGATCCGC CACCAGACCC TCCATCGGCG
  181 GCCGCGCCGC CAGAACCGCC AGTACTATCC AGCCCCAACA GGGGATTCGG GATAGGTTTG
  241 CCATTGCGGG TTTTGTACAC GAAGTAGGTG TAAATCTGTG TCGTCCGAAT CAGAATCTGG
  301 TCCCCGCTGT ATCTGATGGA CCTATACCAC CATGACCCAA CTACTACTGG CAGAATCACC
  361 ATAAAGGCAA GTCCGTAAAC CAGCAGGACC AGAATACTGT TCTTCTGGTC AACGATCCAA
  421 GCAGGAAGGG CGATACCAAA GGAGGTAGCT TGTGGACCAT CAGGATTCCC AAACTCCTCC
  481 CAATTCTTCC TAGACTCTTC GTCAGTCAGT GCTGCATACG CTTTGGCAAT GCGCATAAAC
  541 ATCACCTCAT CGCCTCCTTT ATCGGGGTGA TACTTGAGGC TCAACAATCG ATACTGCTTC
  601 TTGATTTCGG CCACTGTAGC GCCTGGATCA AGATTGAGCA CTTCATACGG GTTGTACTCC
  661 TGATACTCCC TGTCTGTCTT GCTCACTTTG TAGGCCAGAA AGAGAAACAG AGCCCATCCT
  721 GCCAACAGCA CGATTTTCTT GACAGTGGGT ATGATGTTGG GGTTGAGGTT TCAGCAGTCT
  781 AAGGCGGTAC CACATGCACC GTCCATACAC CTTACGAATG TTCTTGAGCC GTATCTGTTC
  841 TGCGTTCTGA TCTCTGGGCC AGAGGTAGTA GGTCGCTGGT ATGACGATCA GGCCGACGAA
  901 GGATGTCAGA AGGTAGAGAA TGTGTTTCCC TGAGTCGTCA TACTGGACTG TGTCGGCATG
  961 GTGGCGGATC GAGCTCGTAT AGCTTAGTTA ACGCTAGCCA GCTGGGTCTC CCTATAGTGA
1021 GTCGTATTAA TCGATAGCCA GTTAGCATTG GTTCTCTAGT G
//
Primer: SV40pro_F
Sequence file: RDB16048_A8I3c.seq check
>D05803_B3_A8I3_1_SV40pro_F_F09_18_ABI24.ab1
    1 CCCGGGTCCT GGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
  961 CGCCCAACCC TGCCATCACG AGATTTCGAT TCCCACCCGC CCGTCTTCTA TGAAGTTTGG
 1021 GCTTCGGCAA TCGGTTTTCC CGGCACGACT GGACTGCAAT GAATCTTCCA GCCGCCGGGG
1081 GATTCTCCAT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl