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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Sec63N-V5-GFP11 (#RDB16048)

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: V5-GFP11; location: endoplasmic reticulum.

Alternative name pMM89, pcDNA3.1_Sec63N-V5-GFP11, pcDNA3.1_Sec63_GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human SEC63 homolog, protein translocation regulator (Sec63), N-terminal segments 1-240 fused with V5-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human SEC63 cDNA
Depositor|Developer Tamura, Yasushi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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提供依頼書  [依頼書の記入例 ]
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RDB16048 Sec63N-V5-GFP11 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Sec63N-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16048).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16048_A8I3p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert 5'(Sec63(1-240) 5')
Sequence file: RDB16048_A8I3d.seq check
>D05803_B3_A8I3_1_CMV-Forward_D09_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTGN NGCNNNGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG
  181 GATCCGCCAC CATGGCCGGA CAACAGTTCC AGTATGACGA CTCAGGGAAC ACCTTCTTCT
  241 ACTTCCTGAC ATCCTTCGTC GGCCTGATCG TCATACCAGC GACCTACTAC CTCTGGCCCA
  301 GAGATCAGAA CGCAGAACAG ATACGGCTCA AGAACATTCG TAAGGTGTAT GGACGGTGCA
  361 TGTGGTACCG CCTTAGACTG CTGAAACCTC AACCCAACAT CATACCCACT GTCAAGAAAA
  421 TCGTGCTGTT GGCAGGATGG GCTCTGTTTC TCTTTCTGGC CTACAAAGTG AGCAAGACAG
  481 ACAGGGAGTA TCAGGAGTAC AACCCGTATG AAGTGCTCAA TCTTGATCCA GGCGCTACAG
  541 TGGCCGAAAT CAAGAAGCAG TATCGATTGT TGAGCCTCAA GTATCACCCC GATAAAGGAG
  601 GCGATGAGGT GATGTTTATG CGCATTGCCA AAGCGTATGC AGCACTGACT GACGAAGAGT
  661 CTAGGAAGAA TTGGGANGAN TTTGGGAATC CTGATGGTCC ACAAGCTACC TCCTTTGGTA
  721 TCGCCCTTCC TGCTTGGATC GTTGACCAGA ANAACAGTAT TCTGGTCCTG CTGGTTTACN
  781 GACTTGCCTT TATGGTGATT CTGCCAGTAN NANTTGGGNC ATGGNGGNAT ANGNCCATCA
  841 GANACNGCGG GNNNNAGANT CNGNNNNNNN NGANNCANNT TTACNCCNAC TTCNNNNNNN
901 AANNCNNNNN NNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA,insert 3'(GFP11,V5,Sec63(1-240) 3')
Sequence file: RDB16048_A8I3e.seq check
>D05803_B3_A8I3_1_BGH_rev2_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNGGGGG NNGGNNNNNN ACAGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGATTATG TAATCCCAGC AGCATTTACG
  121 TACTCATGAA GGACCATGTG GTCACGACTT GTTGATCCGC CACCAGACCC TCCATCGGCG
  181 GCCGCGCCGC CAGAACCGCC AGTACTATCC AGCCCCAACA GGGGATTCGG GATAGGTTTG
  241 CCATTGCGGG TTTTGTACAC GAAGTAGGTG TAAATCTGTG TCGTCCGAAT CAGAATCTGG
  301 TCCCCGCTGT ATCTGATGGA CCTATACCAC CATGACCCAA CTACTACTGG CAGAATCACC
  361 ATAAAGGCAA GTCCGTAAAC CAGCAGGACC AGAATACTGT TCTTCTGGTC AACGATCCAA
  421 GCAGGAAGGG CGATACCAAA GGAGGTAGCT TGTGGACCAT CAGGATTCCC AAACTCCTCC
  481 CAATTCTTCC TAGACTCTTC GTCAGTCAGT GCTGCATACG CTTTGGCAAT GCGCATAAAC
  541 ATCACCTCAT CGCCTCCTTT ATCGGGGTGA TACTTGAGGC TCAACAATCG ATACTGCTTC
  601 TTGATTTCGG CCACTGTAGC GCCTGGATCA AGANTGAGCA CTTCATACGG GTTGTACTCC
  661 TGATACTCCC TGTCTGTCTT GCTCACTTTG TANGCCAGAA AGAGAAACAG AGCCCATCCT
  721 GCCAACAGCA CGATTTTCTT GACAGTGGGT ATGANGNTGG GGTTGANGNT TCAGCAGTCT
  781 AANGCGGTAC CNCATGCACN NTCCNTACNC CTNACGANNG NTCNNGANCN NTANCTGNNC
841 TGCGTTCNGA TNNCNGGNNN NNANNNAGTN NNNCGCNNGN ATGNNNANNN NNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB16048_A8I3f.seq check
>D05803_B3_A8I3_1_SV40pro_F_F09_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNTGNN GNNGCTTTTT TGGNNGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGNGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGANGNTCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGANTCATCG ACTGTNGCCG GCTGGGTGTG GCGGANCGCT
  781 ATCANGNNAT AGCGTTGGCT ANNNGTGATA TTGCTGAAGA NCTTGGCGGC GAANGGGCTG
  841 NNCGCNTCCN CGNGCTTTAC NGNANCGCNN NNNCNNNNNC NNANCGCATC GNNTNNTANC
901 NNCTNNNNNN NGANTNCTTN NNANCNGNNN NNNNGGGNNN NNNANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]