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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

ERj1N-V5-GFP11 (#RDB16047)

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: V5-GFP11; location: endoplasmic reticulum.

Alternative name pMM87, pcDNA3.1_ERj1N-V5-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1, N-terminal segments 1-200 fused with V5-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human DnaJ Heat Shock Protein Family (Hsp40) Member C1 cDNA
Depositor|Developer Tamura, Yasushi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

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RDB16047 ERj1N-V5-GFP11 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The ERj1N-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16047).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16047_A8I3p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(ERj1(1-200))
Sequence file: RDB16047_A8I3d.seq check
>D05803_B2_A8I3_1_CMV-Forwrd_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNTTNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGNN GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG
  181 ATCCGCCACC ATGACTGCTC CGTGCTCTCA ACCCGCACAA CTGCCAGGAC GGAGACAGCT
  241 GGGTCTGGTA CCTTTTCCTC CTCCACCACC ACGTACACCT CTGCTGTGGT TGCTGCTTCT
  301 GCTGCTTGCT GCGGTTGCAC CGGCTAGAGG ATGGGAATCC GGCGATTTGG AGCTGTTTGA
  361 CCTGGTCGAA GAAGTGCAGT TGAACTTCTA CCAGTTTCTC GGGGTACAAC AGGATGCCAG
  421 CTCAGCTGAT ATCCGCAAAG CCTATCGGAA ATTGTCCCTC ACACTGCATC CCGATAAGAA
  481 CAAAGACGAG AATGCCGAAA CCCAGTTCAG ACAGCTCGTG GCCATTTACG AGGTCCTGAA
  541 AGACGATGAG AGGAGACAGA GGTATGACGA CATTCTGATC AACGGACTGC CCGACTGGCG
  601 ACAGCCTGTG TTCTACTATA GGCGAGTGCG GAAGATGTCC AATGCCGAGC TTGCCCTGCT
  661 TCTCTTCATC ATTCTCACGG TTGGCCACTA TGCAGTCGTG TGGAGCATCT ACCTGGANAA
  721 ACAGCTGGAT GANCTCCTGT CACGCAAGAA GCGCGAAAAG AAGAAGAAAA CCGGCAGTAA
  781 GANCGTGGAT GGGAAANNCA TACCCNATCN ACTCCNGGGN CTTGNNTCTA CTNGNNGGNN
  841 TGGCGGCGNN GNCNNCNATN GANGNNCNNG NNGCGGNTCA ACAANNCNNN NNNNCNNNGN
901 NNCTNNANNN NNACNNNANN NNNNNNNGNN NNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA,insert 3'(GFP11,V5, ERj1(1-200))
Sequence file: RDB16047_A8I3e.seq check
>D05803_B2_A8I3_1_BGH_rev2_B09_06_ABI24.ab1
    1 NNNNNNNNNN GNNNNNNGGN GGNNGGGCNN ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGATT ATGTAATCCC AGCAGCATTT
  121 ACGTACTCAT GAAGGACCAT GTGGTCACGA CTTGTTGATC CGCCACCAGA CCCTCCATCG
  181 GCGGCCGCGC CGCCACTCCC TCCAGTAGAG TCAAGACCCA GGAGTGGATT GGGTATGGGT
  241 TTCCCATCCA CGCTCTTACT GCCGGTTTTC TTCTTCTTTT CGCGCTTCTT GCGTGACAGG
  301 AGCTCATCCA GCTGTTTCTC CAGGTAGATG CTCCACACGA CTGCATAGTG GCCAACCGTG
  361 AGAATGATGA AGAGAAGCAG GGCAAGCTCG GCATTGGACA TCTTCCGCAC TCGCCTATAG
  421 TAGAACACAG GCTGTCGCCA GTCGGGCAGT CCGTTGATCA GAATGTCGTC ATACCTCTGT
  481 CTCCTCTCAT CGTCTTTCAG GACCTCGTAA ATGGCCACGA GCTGTCTGAA CTGGGTTTCG
  541 GCATTCTCGT CTTTGTTCTT ATCGGGATGC AGTGTGAGGG ACAATTTCCG ATAGGCTTTG
  601 CGGATATCAG CTGAGCTGGC ATCCTGTTGT ACCCCGAGAA ACTGGTAGAA GTTCAACTGC
  661 ACTTCTTCGA CCAGGTCAAA CAGCTCCAAA TCGCCGGANT CCCATCCTCT AGCCGGTGCA
  721 ACCGCAGCAA GCAGCAGAAG CAGCAACCAC AGCAGANGTG TACGTGGTGG TGGAGGANGA
  781 AAAGGNACCA GANNCAGCTG TCTNCGNCCT GNNNGTTGTG CGGGTTGAGA NCACGGNNCA
  841 NNCATGGNGG CGGNTNCNAN CTCNGNANCN AGCTTNAGTT NAAACGCTAN CCAGCTNNGG
901 NNNNCNNNNN GTGANNNNNA NTNATTTCNA NNNNNCNNNN NNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB16047_A8I3f.seq check
>D05803_B2_A8I3_1_SV40pro_F_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNANN NNTGNNGNNG CTTTTTTGGA NGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGN GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GNTCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 NNNTGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 NGNCATAGCG TTNGCTACNN GNGATATTGC TGAANANCTT GGCGGCNNAT GNNCTGACCG
  841 CTTCCTCGNG CTTTACNGNA TCGCNGCNNC CNANTCNCAN CGCATCNCCT TCNATNGCCT
901 TCTNNNNGAN TTNTTCTGAN CNGGNNNNNN NGGGGTNNNN AATNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]