The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: V5-GFP11; location: endoplasmic reticulum.
Alternative name | pMM87, pcDNA3.1_ERj1N-V5-GFP11 |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1, N-terminal segments 1-200 fused with V5-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pcDNA3.1 (plasmid) |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | human DnaJ Heat Shock Protein Family (Hsp40) Member C1 cDNA |
Depositor|Developer | Tamura, Yasushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16047 | ERj1N-V5-GFP11 | DNA solution |
Materials & Methods section:
The ERj1N-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16047). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16047_A8I3p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(ERj1(1-200)) Sequence file: RDB16047_A8I3d.seq ![]() |
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>D05803_B2_A8I3_1_CMV-Forwrd_H03_24_ABI24.ab1 1 NNNNNNNNNN NNNNNTTNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGNN GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG 181 ATCCGCCACC ATGACTGCTC CGTGCTCTCA ACCCGCACAA CTGCCAGGAC GGAGACAGCT 241 GGGTCTGGTA CCTTTTCCTC CTCCACCACC ACGTACACCT CTGCTGTGGT TGCTGCTTCT 301 GCTGCTTGCT GCGGTTGCAC CGGCTAGAGG ATGGGAATCC GGCGATTTGG AGCTGTTTGA 361 CCTGGTCGAA GAAGTGCAGT TGAACTTCTA CCAGTTTCTC GGGGTACAAC AGGATGCCAG 421 CTCAGCTGAT ATCCGCAAAG CCTATCGGAA ATTGTCCCTC ACACTGCATC CCGATAAGAA 481 CAAAGACGAG AATGCCGAAA CCCAGTTCAG ACAGCTCGTG GCCATTTACG AGGTCCTGAA 541 AGACGATGAG AGGAGACAGA GGTATGACGA CATTCTGATC AACGGACTGC CCGACTGGCG 601 ACAGCCTGTG TTCTACTATA GGCGAGTGCG GAAGATGTCC AATGCCGAGC TTGCCCTGCT 661 TCTCTTCATC ATTCTCACGG TTGGCCACTA TGCAGTCGTG TGGAGCATCT ACCTGGANAA 721 ACAGCTGGAT GANCTCCTGT CACGCAAGAA GCGCGAAAAG AAGAAGAAAA CCGGCAGTAA 781 GANCGTGGAT GGGAAANNCA TACCCNATCN ACTCCNGGGN CTTGNNTCTA CTNGNNGGNN 841 TGGCGGCGNN GNCNNCNATN GANGNNCNNG NNGCGGNTCA ACAANNCNNN NNNNCNNNGN 901 NNCTNNANNN NNACNNNANN NNNNNNNGNN NNNNNNNN // |
Primer: BGH_rev2 (Pr0606) Region: bGH pA,insert 3'(GFP11,V5, ERj1(1-200)) Sequence file: RDB16047_A8I3e.seq ![]() |
>D05803_B2_A8I3_1_BGH_rev2_B09_06_ABI24.ab1 1 NNNNNNNNNN GNNNNNNGGN GGNNGGGCNN ACAACAGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGATT ATGTAATCCC AGCAGCATTT 121 ACGTACTCAT GAAGGACCAT GTGGTCACGA CTTGTTGATC CGCCACCAGA CCCTCCATCG 181 GCGGCCGCGC CGCCACTCCC TCCAGTAGAG TCAAGACCCA GGAGTGGATT GGGTATGGGT 241 TTCCCATCCA CGCTCTTACT GCCGGTTTTC TTCTTCTTTT CGCGCTTCTT GCGTGACAGG 301 AGCTCATCCA GCTGTTTCTC CAGGTAGATG CTCCACACGA CTGCATAGTG GCCAACCGTG 361 AGAATGATGA AGAGAAGCAG GGCAAGCTCG GCATTGGACA TCTTCCGCAC TCGCCTATAG 421 TAGAACACAG GCTGTCGCCA GTCGGGCAGT CCGTTGATCA GAATGTCGTC ATACCTCTGT 481 CTCCTCTCAT CGTCTTTCAG GACCTCGTAA ATGGCCACGA GCTGTCTGAA CTGGGTTTCG 541 GCATTCTCGT CTTTGTTCTT ATCGGGATGC AGTGTGAGGG ACAATTTCCG ATAGGCTTTG 601 CGGATATCAG CTGAGCTGGC ATCCTGTTGT ACCCCGAGAA ACTGGTAGAA GTTCAACTGC 661 ACTTCTTCGA CCAGGTCAAA CAGCTCCAAA TCGCCGGANT CCCATCCTCT AGCCGGTGCA 721 ACCGCAGCAA GCAGCAGAAG CAGCAACCAC AGCAGANGTG TACGTGGTGG TGGAGGANGA 781 AAAGGNACCA GANNCAGCTG TCTNCGNCCT GNNNGTTGTG CGGGTTGAGA NCACGGNNCA 841 NNCATGGNGG CGGNTNCNAN CTCNGNANCN AGCTTNAGTT NAAACGCTAN CCAGCTNNGG 901 NNNNCNNNNN GTGANNNNNA NTNATTTCNA NNNNNCNNNN NNNNNN // |
Primer: SV40pro_F (Pr0110) Region: NeoR Sequence file: RDB16047_A8I3f.seq ![]() |
>D05803_B2_A8I3_1_SV40pro_F_C09_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNANN NNTGNNGNNG CTTTTTTGGA NGCCTAGGCT TTTGCAAAAA 61 GCTCCCGGGN GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT 121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC 661 CGACGGCGAG GNTCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 721 NNNTGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 781 NGNCATAGCG TTNGCTACNN GNGATATTGC TGAANANCTT GGCGGCNNAT GNNCTGACCG 841 CTTCCTCGNG CTTTACNGNA TCGCNGCNNC CNANTCNCAN CGCATCNCCT TCNATNGCCT 901 TCTNNNNGAN TTNTTCTGAN CNGGNNNNNN NGGGGTNNNN AATNNNNNNN NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
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