Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

ERj1N-V5-GFP11

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: V5-GFP11; location: endoplasmic reticulum.

Catalog number RDB16047
Resource name ERj1N-V5-GFP11
Alternative name pMM87, pcDNA3.1_ERj1N-V5-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1, N-terminal segments 1-200 fused with V5-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human DnaJ Heat Shock Protein Family (Hsp40) Member C1 cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
human DnaJ Heat Shock Protein Family (Hsp40) Member C1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16047 ERj1N-V5-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The ERj1N-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16047).

          Reference section:

          Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16047_A8I3p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'(ERj1(1-200))
          Sequence file: RDB16047_A8I3d.seq check
          >D05803_B2_A8I3_1_CMV-Forwrd_H03_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNTTNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGNN GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG
            181 ATCCGCCACC ATGACTGCTC CGTGCTCTCA ACCCGCACAA CTGCCAGGAC GGAGACAGCT
            241 GGGTCTGGTA CCTTTTCCTC CTCCACCACC ACGTACACCT CTGCTGTGGT TGCTGCTTCT
            301 GCTGCTTGCT GCGGTTGCAC CGGCTAGAGG ATGGGAATCC GGCGATTTGG AGCTGTTTGA
            361 CCTGGTCGAA GAAGTGCAGT TGAACTTCTA CCAGTTTCTC GGGGTACAAC AGGATGCCAG
            421 CTCAGCTGAT ATCCGCAAAG CCTATCGGAA ATTGTCCCTC ACACTGCATC CCGATAAGAA
            481 CAAAGACGAG AATGCCGAAA CCCAGTTCAG ACAGCTCGTG GCCATTTACG AGGTCCTGAA
            541 AGACGATGAG AGGAGACAGA GGTATGACGA CATTCTGATC AACGGACTGC CCGACTGGCG
            601 ACAGCCTGTG TTCTACTATA GGCGAGTGCG GAAGATGTCC AATGCCGAGC TTGCCCTGCT
            661 TCTCTTCATC ATTCTCACGG TTGGCCACTA TGCAGTCGTG TGGAGCATCT ACCTGGANAA
            721 ACAGCTGGAT GANCTCCTGT CACGCAAGAA GCGCGAAAAG AAGAAGAAAA CCGGCAGTAA
            781 GANCGTGGAT GGGAAANNCA TACCCNATCN ACTCCNGGGN CTTGNNTCTA CTNGNNGGNN
            841 TGGCGGCGNN GNCNNCNATN GANGNNCNNG NNGCGGNTCA ACAANNCNNN NNNNCNNNGN
          901 NNCTNNANNN NNACNNNANN NNNNNNNGNN NNNNNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,insert 3'(GFP11,V5, ERj1(1-200))
          Sequence file: RDB16047_A8I3e.seq check
          >D05803_B2_A8I3_1_BGH_rev2_B09_06_ABI24.ab1
              1 NNNNNNNNNN GNNNNNNGGN GGNNGGGCNN ACAACAGATG GCTGGCAACT AGAAGGCACA
             61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGATT ATGTAATCCC AGCAGCATTT
            121 ACGTACTCAT GAAGGACCAT GTGGTCACGA CTTGTTGATC CGCCACCAGA CCCTCCATCG
            181 GCGGCCGCGC CGCCACTCCC TCCAGTAGAG TCAAGACCCA GGAGTGGATT GGGTATGGGT
            241 TTCCCATCCA CGCTCTTACT GCCGGTTTTC TTCTTCTTTT CGCGCTTCTT GCGTGACAGG
            301 AGCTCATCCA GCTGTTTCTC CAGGTAGATG CTCCACACGA CTGCATAGTG GCCAACCGTG
            361 AGAATGATGA AGAGAAGCAG GGCAAGCTCG GCATTGGACA TCTTCCGCAC TCGCCTATAG
            421 TAGAACACAG GCTGTCGCCA GTCGGGCAGT CCGTTGATCA GAATGTCGTC ATACCTCTGT
            481 CTCCTCTCAT CGTCTTTCAG GACCTCGTAA ATGGCCACGA GCTGTCTGAA CTGGGTTTCG
            541 GCATTCTCGT CTTTGTTCTT ATCGGGATGC AGTGTGAGGG ACAATTTCCG ATAGGCTTTG
            601 CGGATATCAG CTGAGCTGGC ATCCTGTTGT ACCCCGAGAA ACTGGTAGAA GTTCAACTGC
            661 ACTTCTTCGA CCAGGTCAAA CAGCTCCAAA TCGCCGGANT CCCATCCTCT AGCCGGTGCA
            721 ACCGCAGCAA GCAGCAGAAG CAGCAACCAC AGCAGANGTG TACGTGGTGG TGGAGGANGA
            781 AAAGGNACCA GANNCAGCTG TCTNCGNCCT GNNNGTTGTG CGGGTTGAGA NCACGGNNCA
            841 NNCATGGNGG CGGNTNCNAN CTCNGNANCN AGCTTNAGTT NAAACGCTAN CCAGCTNNGG
          901 NNNNCNNNNN GTGANNNNNA NTNATTTCNA NNNNNCNNNN NNNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB16047_A8I3f.seq check
          >D05803_B2_A8I3_1_SV40pro_F_C09_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNANN NNTGNNGNNG CTTTTTTGGA NGCCTAGGCT TTTGCAAAAA
             61 GCTCCCGGGN GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
            121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
            181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
            241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
            301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
            361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
            421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
            481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
            541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
            601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
            661 CGACGGCGAG GNTCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
            721 NNNTGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
            781 NGNCATAGCG TTNGCTACNN GNGATATTGC TGAANANCTT GGCGGCNNAT GNNCTGACCG
            841 CTTCCTCGNG CTTTACNGNA TCGCNGCNNC CNANTCNCAN CGCATCNCCT TCNATNGCCT
          901 TCTNNNNGAN TTNTTCTGAN CNGGNNNNNN NGGGGTNNNN AATNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl