Please review the QC test results indicated by icon below as well as clone information before placing your order.

ERj1N-V5-GFP11

Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1, N-terminal segments 1-200 for visualizing organelle contact sites in vivo.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16047_A8I3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB16047_A8I3a.seq
>D05803_B2_A8I3_1_CMV-Forwrd_H03_24_ABI24.ab1     1 CATCACTCCG GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA   121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG   181 ATCCGCCACC ATGACTGCTC CGTGCTCTCA ACCCGCACAA CTGCCAGGAC GGAGACAGCT   241 GGGTCTGGTA CCTTTTCCTC CTCCACCACC ACGTACACCT CTGCTGTGGT TGCTGCTTCT   301 GCTGCTTGCT GCGGTTGCAC CGGCTAGAGG ATGGGAATCC GGCGATTTGG AGCTGTTTGA   361 CCTGGTCGAA GAAGTGCAGT TGAACTTCTA CCAGTTTCTC GGGGTACAAC AGGATGCCAG   421 CTCAGCTGAT ATCCGCAAAG CCTATCGGAA ATTGTCCCTC ACACTGCATC CCGATAAGAA   481 CAAAGACGAG AATGCCGAAA CCCAGTTCAG ACAGCTCGTG GCCATTTACG AGGTCCTGAA   541 AGACGATGAG AGGAGACAGA GGTATGACGA CATTCTGATC AACGGACTGC CCGACTGGCG   601 ACAGCCTGTG TTCTACTATA GGCGAGTGCG GAAGATGTCC AATGCCGAGC TTGCCCTGCT   661 TCTCTTCATC ATTCTCACGG TTGGCCACTA TGCAGTCGTG TGGAGCATCT ACCTGGAGAA   721 ACAGCTGGAT GAGCTCCTGT CACGCAAGAA GCGCGAAAAG AAGAAGAAAA CCGGCAGTAA   781 GAGCGTGGAT GGGAAACCCA TACCCAATCC ACTCCTGGGT CTTGACTCTA CTGGAGGGAG   841 TGGCGGCGCG GCCGCCGATG GAGGGTCTGG TGGCGGATCA ACAAGTCGTG ACCACATGGG   901 TCCTTCATGA GTACGTAAAT GCTGCTGGGA TTACATAATC TAGAGGGCCC GTTTAAACCC   961 GCTGATCAGC CCTCGACTGT GCCCTTCTAG TTTGCCCAGT CATTCTTGCT GATTTGCCCC 1021 CTCCCCCGGT GCGTTTCGAT GACCTGGCAT GCTGCACTTC CCACTGATTC CGTTTCCTA //
Primer: BGH_rev2 Sequence file: RDB16047_A8I3b.seq
>D05803_B2_A8I3_1_BGH_rev2_B09_06_ABI24.ab1     1 CTCAGGTTCA GAAGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGATT ATGTAATCCC AGCAGCATTT   121 ACGTACTCAT GAAGGACCAT GTGGTCACGA CTTGTTGATC CGCCACCAGA CCCTCCATCG   181 GCGGCCGCGC CGCCACTCCC TCCAGTAGAG TCAAGACCCA GGAGTGGATT GGGTATGGGT   241 TTCCCATCCA CGCTCTTACT GCCGGTTTTC TTCTTCTTTT CGCGCTTCTT GCGTGACAGG   301 AGCTCATCCA GCTGTTTCTC CAGGTAGATG CTCCACACGA CTGCATAGTG GCCAACCGTG   361 AGAATGATGA AGAGAAGCAG GGCAAGCTCG GCATTGGACA TCTTCCGCAC TCGCCTATAG   421 TAGAACACAG GCTGTCGCCA GTCGGGCAGT CCGTTGATCA GAATGTCGTC ATACCTCTGT   481 CTCCTCTCAT CGTCTTTCAG GACCTCGTAA ATGGCCACGA GCTGTCTGAA CTGGGTTTCG   541 GCATTCTCGT CTTTGTTCTT ATCGGGATGC AGTGTGAGGG ACAATTTCCG ATAGGCTTTG   601 CGGATATCAG CTGAGCTGGC ATCCTGTTGT ACCCCGAGAA ACTGGTAGAA GTTCAACTGC   661 ACTTCTTCGA CCAGGTCAAA CAGCTCCAAA TCGCCGGATT CCCATCCTCT AGCCGGTGCA   721 ACCGCAGCAA GCAGCAGAAG CAGCAACCAC AGCAGAGGTG TACGTGGTGG TGGAGGAGGA   781 AAAGGTACCA GACCCAGCTG TCTCCGTCCT GGCAGTTGTG CGGGTTGAGA GCACGGAGCA   841 GTCATGGTGG CGGATCCGAG CTCGGTACCA AGCTTAAGTT TAAACGCTAG CCAGCTTGGG   901 TCTCCCTATA GTGAGTCGTA TTAATTTCGA TAAGCCCAGT AAGCAGTGGG TTTCTCTAGT   961 TTAGCCAGAG AGCTCTGCTT TATATAGACC TCCCACCGTA CACGCCTACC CGCCCATTTG  1021 CGTCCATGGG GGCGGAGTGG TACGGACATT TTGGGAAAGT CCCGATTGAA TTTTGATGAC 1081 TAAACCAAAA CTTCCCAACT GACGGGC //
Primer: SV40pro_F Sequence file: RDB16047_A8I3c.seq
>D05803_B2_A8I3_1_SV40pro_F_C09_09_ABI24.ab1     1 ACTGTCCTGG CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA    61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT   121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT   181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT   241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA   301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC   361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG   421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC   481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA   541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA   601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC   661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA   721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA   781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG   841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT   901 TCTTGACGAG TTCTTCTGAG CGGGGACTCT GGGGGTTCGA AATGACCGAC CAAAGCGACG   961 CCCCAACCTG CCATCACGAG ATTTTCGATT CCCACCGCCG CCCTTCCTAT GAAAGGTTGG  1021 GGCTTCGGAG TCGTTTTCCG GGGACGTCGG ACTGGTATGA TCCTTCCAAG CCGGCCGGGG 1081 GGAATCCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl