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ERj1N-V5-GFP11

Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1, N-terminal segments 1-200 for visualizing organelle contact sites in vivo.

Catalog number RDB16047
Resource name ERj1N-V5-GFP11
Alternative name pcDNA3.1_ERj1N-V5-GFP11 (pMM87)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1, N-terminal segments 1-200 fused with V5-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human DnaJ Heat Shock Protein Family (Hsp40) Member C1 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank human DNAJC1 (NCBI Gene 64215) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
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RDB16047 ERj1N-V5-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16047_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB16047_A8I3a.seq check
>D05803_B2_A8I3_1_CMV-Forwrd_H03_24_ABI24.ab1
    1 CATCACTCCG GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG
  181 ATCCGCCACC ATGACTGCTC CGTGCTCTCA ACCCGCACAA CTGCCAGGAC GGAGACAGCT
  241 GGGTCTGGTA CCTTTTCCTC CTCCACCACC ACGTACACCT CTGCTGTGGT TGCTGCTTCT
  301 GCTGCTTGCT GCGGTTGCAC CGGCTAGAGG ATGGGAATCC GGCGATTTGG AGCTGTTTGA
  361 CCTGGTCGAA GAAGTGCAGT TGAACTTCTA CCAGTTTCTC GGGGTACAAC AGGATGCCAG
  421 CTCAGCTGAT ATCCGCAAAG CCTATCGGAA ATTGTCCCTC ACACTGCATC CCGATAAGAA
  481 CAAAGACGAG AATGCCGAAA CCCAGTTCAG ACAGCTCGTG GCCATTTACG AGGTCCTGAA
  541 AGACGATGAG AGGAGACAGA GGTATGACGA CATTCTGATC AACGGACTGC CCGACTGGCG
  601 ACAGCCTGTG TTCTACTATA GGCGAGTGCG GAAGATGTCC AATGCCGAGC TTGCCCTGCT
  661 TCTCTTCATC ATTCTCACGG TTGGCCACTA TGCAGTCGTG TGGAGCATCT ACCTGGAGAA
  721 ACAGCTGGAT GAGCTCCTGT CACGCAAGAA GCGCGAAAAG AAGAAGAAAA CCGGCAGTAA
  781 GAGCGTGGAT GGGAAACCCA TACCCAATCC ACTCCTGGGT CTTGACTCTA CTGGAGGGAG
  841 TGGCGGCGCG GCCGCCGATG GAGGGTCTGG TGGCGGATCA ACAAGTCGTG ACCACATGGG
  901 TCCTTCATGA GTACGTAAAT GCTGCTGGGA TTACATAATC TAGAGGGCCC GTTTAAACCC
  961 GCTGATCAGC CCTCGACTGT GCCCTTCTAG TTTGCCCAGT CATTCTTGCT GATTTGCCCC
1021 CTCCCCCGGT GCGTTTCGAT GACCTGGCAT GCTGCACTTC CCACTGATTC CGTTTCCTA
//
Primer: BGH_rev2
Sequence file: RDB16047_A8I3b.seq check
>D05803_B2_A8I3_1_BGH_rev2_B09_06_ABI24.ab1
    1 CTCAGGTTCA GAAGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGATT ATGTAATCCC AGCAGCATTT
  121 ACGTACTCAT GAAGGACCAT GTGGTCACGA CTTGTTGATC CGCCACCAGA CCCTCCATCG
  181 GCGGCCGCGC CGCCACTCCC TCCAGTAGAG TCAAGACCCA GGAGTGGATT GGGTATGGGT
  241 TTCCCATCCA CGCTCTTACT GCCGGTTTTC TTCTTCTTTT CGCGCTTCTT GCGTGACAGG
  301 AGCTCATCCA GCTGTTTCTC CAGGTAGATG CTCCACACGA CTGCATAGTG GCCAACCGTG
  361 AGAATGATGA AGAGAAGCAG GGCAAGCTCG GCATTGGACA TCTTCCGCAC TCGCCTATAG
  421 TAGAACACAG GCTGTCGCCA GTCGGGCAGT CCGTTGATCA GAATGTCGTC ATACCTCTGT
  481 CTCCTCTCAT CGTCTTTCAG GACCTCGTAA ATGGCCACGA GCTGTCTGAA CTGGGTTTCG
  541 GCATTCTCGT CTTTGTTCTT ATCGGGATGC AGTGTGAGGG ACAATTTCCG ATAGGCTTTG
  601 CGGATATCAG CTGAGCTGGC ATCCTGTTGT ACCCCGAGAA ACTGGTAGAA GTTCAACTGC
  661 ACTTCTTCGA CCAGGTCAAA CAGCTCCAAA TCGCCGGATT CCCATCCTCT AGCCGGTGCA
  721 ACCGCAGCAA GCAGCAGAAG CAGCAACCAC AGCAGAGGTG TACGTGGTGG TGGAGGAGGA
  781 AAAGGTACCA GACCCAGCTG TCTCCGTCCT GGCAGTTGTG CGGGTTGAGA GCACGGAGCA
  841 GTCATGGTGG CGGATCCGAG CTCGGTACCA AGCTTAAGTT TAAACGCTAG CCAGCTTGGG
  901 TCTCCCTATA GTGAGTCGTA TTAATTTCGA TAAGCCCAGT AAGCAGTGGG TTTCTCTAGT
  961 TTAGCCAGAG AGCTCTGCTT TATATAGACC TCCCACCGTA CACGCCTACC CGCCCATTTG
 1021 CGTCCATGGG GGCGGAGTGG TACGGACATT TTGGGAAAGT CCCGATTGAA TTTTGATGAC
1081 TAAACCAAAA CTTCCCAACT GACGGGC
//
Primer: SV40pro_F
Sequence file: RDB16047_A8I3c.seq check
>D05803_B2_A8I3_1_SV40pro_F_C09_09_ABI24.ab1
    1 ACTGTCCTGG CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  901 TCTTGACGAG TTCTTCTGAG CGGGGACTCT GGGGGTTCGA AATGACCGAC CAAAGCGACG
  961 CCCCAACCTG CCATCACGAG ATTTTCGATT CCCACCGCCG CCCTTCCTAT GAAAGGTTGG
 1021 GGCTTCGGAG TCGTTTTCCG GGGACGTCGG ACTGGTATGA TCCTTCCAAG CCGGCCGGGG
1081 GGAATCCT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl