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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Tom20N-FLAG-GFP1-10

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: 3xFLAG-GFP1-10; location: mitochondria.

Catalog number RDB16041
Resource name Tom20N-FLAG-GFP1-10
Alternative name pMM80, pcDNA3.1_Tom20N-3xFLAG-GFP1-10, pcDNA3.1_Tom20N-FLAG-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 fused with3XFLAG-GFP1-10 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig. S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human TOMM20 cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
human TOMM20

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
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          RDB16041 Tom20N-FLAG-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Tom20N-FLAG-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16041).

          Reference section:

          Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Fluorescent protein probe that can visualize the multiple inter-organelle contact sites in cells (English text)
          Featured content Fluorescent protein probe that can visualize the multiple inter-organelle contact sites in cells (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16041_A8I3p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,T7 pro,insert 5'(Tom20(1-33),3XFLAG,GFP1-10 5')
          Sequence file: RDB16041_A8I3d.seq check
          >D05803_A5_A8I3_1_CMV-Forward_A08_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NGCNNNGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG
            181 GATCCGCCAC CATGGTGGGC AGGAACAGCG CCATCGCCGC CGGCGTGTGC GGCGCCCTGT
            241 TCATCGGCTA CTGTATCTAT TTTGACCGGA AGCGGAGATC CGACCCCAAT GATTACAAGG
            301 ACCACGATGG CGACTATAAG GATCACGACA TCGATTACAA GGACGATGAC GATAAGGGCG
            361 GCTCTGGCGC GGCCGCCGGT GGCACTAGTA TGAGCAAAGG AGAAGAACTT TTCACTGGAG
            421 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGAG
            481 GAGAGGGTGA AGGTGATGCT ACAATCGGAA AACTCACACT TAAATTTATT TGCACTACTG
            541 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTCT GACCTATGGT GTTCAATGCT
            601 TTTCCCGTTA TCCGGATCAC ATGAAAAGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG
            661 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAAATACAAG ACGCGTGCTG
            721 TAGTCAAGTT TGAAGGTGAT ACCNTTGTTA ATCGTATCGA GTTAAAGGGT ACTGATTTTA
            781 AAGAAGATGG AAACATTCTC GGACACAAAC TCGNNTACAA CTTTAACTCA CACAANGTAT
            841 ACATCACGGC AGACAAACAA AAGANTGGAA TCNAAGCTAA CTTCACAGTT CGCCNCAACG
          901 NNNAANATGG NNCCGTNCAN NNNNNNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,insert3'(GFP1-10)
          Sequence file: RDB16041_A8I3e.seq check
          >D05803_A5_A8I3_1_BGH_rev2_B08_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGGNG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGATTAC TTTTCGTTGG GATCTTTCGA
            121 AAGGACAGTT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC CATCGCCAAT
            181 TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAA CCATCTTCAA CGTTGTGGCG
            241 AACTGTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT ATACATTGTG
            301 TGAGTTAAAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT TAAAATCAGT
            361 ACCCTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTA CAGCACGCGT
            421 CTTGTATTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC CTTCGGGCAT
            481 GGCACTCTTG AAAAAGTCAT GCCTTTTCAT GTGATCCGGA TAACGGGAAA AGCATTGAAC
            541 ACCATAGGTC AGAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC CAGTAGTGCA
            601 AATAAATTTA AGTGTGAGTT TTCCGATTGT AGCATCACCT TCACCCTCTC CTCTGACAGA
            661 AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA
            721 AAGTTCTTCT CCTTTGCTCA TACTAGTGCC ACCGGCGGCC GCGCCAGANC CGCCCTTATC
            781 GTCATCGTCC TTGTAATCGA TGTCGTGATC CTTATAGTCG CCATCGNGGN CCTTGTAATC
            841 ATTGGGGGTC GGNTCNNNNC TNNCGNNCAA AATAGATACA GTAGCCNATG ANCAGGGNGC
          901 CNCACACGCC NGCGGNNNNN GCGCTNNNCC NGNNNNCNNN NNNGNNNNNN NNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB16041_A8I3f.seq check
          >D05803_A5_A8I3_1_SV40pro_F_C08_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNA NNNNTGNNGN NGCTTTTTTG GANGCCTAGG CTTTTGCNAA
             61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
            121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
            661 CCCGACGGCG AGGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
            781 CANGNCATAG CGTTGGCTAC NCGNGNTATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAN
            841 CGCTTCCTCG NGCTTTACGG TATCGCCGCN CCCNATTCGC AGCGCATCNC CNTCNANNGC
          901 CTTCTNNNCN ANTNCTNCNN NNCGGGANTN NNGGGGNNCN NANTGANNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

          2024.03.26

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