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Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 for visualizing organelle contact sites in vivo.
| Catalog number | RDB16041 |
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| Resource name | Tom20N-FLAG-GFP1-10 |
| Alternative name | pcDNA3.1_Tom20N-FLAG-GFP1-10 (pMM80) |
| Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 fused with3XFLAG-GFP1-10 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig. S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. Expression was confirmed by the depositor with fluorescence microscopy. |
| Vector backbone | pcDNA3.1 (plasmid) |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells) |
| Gene/insert name | human TOMM20 cDNA |
| Depositor|Developer | Tamura, Yasushi | |
| Other clones in our bank | human TOMM20 (NCBI Gene 9804) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
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| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer or Check Payment]Material Transfer Agreement (MTA) [Word ]Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB16041 | Tom20N-FLAG-GFP1-10 | DNA solution |
icon below as well as clone information before placing your order.Electronic file
Original reference
| original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Dnaconda's recommendation EXR0090j (Japanese text) |
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| Featured content | Dnaconda's recommendation EXR0090e (English text) |
| Featured content | Visualization of Organelles (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB16041_A8I3p1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV_Forward Region: CMV pro,T7 pro,insert5'(Tom20(1-33),3XFLAG,GFP1-10) Sequence file: RDB16041_A8I3a.seq
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>D05803_A5_A8I3_1_CMV-Forward_A08_02_ABI24.ab1 1 CTTCAACTTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG 121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG 181 GATCCGCCAC CATGGTGGGC AGGAACAGCG CCATCGCCGC CGGCGTGTGC GGCGCCCTGT 241 TCATCGGCTA CTGTATCTAT TTTGACCGGA AGCGGAGATC CGACCCCAAT GATTACAAGG 301 ACCACGATGG CGACTATAAG GATCACGACA TCGATTACAA GGACGATGAC GATAAGGGCG 361 GCTCTGGCGC GGCCGCCGGT GGCACTAGTA TGAGCAAAGG AGAAGAACTT TTCACTGGAG 421 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGAG 481 GAGAGGGTGA AGGTGATGCT ACAATCGGAA AACTCACACT TAAATTTATT TGCACTACTG 541 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTCT GACCTATGGT GTTCAATGCT 601 TTTCCCGTTA TCCGGATCAC ATGAAAAGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG 661 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAAATACAAG ACGCGTGCTG 721 TAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATCGTATCGA GTTAAAGGGT ACTGATTTTA 781 AAGAAGATGG AAACATTCTC GGACACAAAC TCGAGTACAA CTTTAACTCA CACAATGTAT 841 ACATCACGGC AGACAAACAA AAGAATGGAA TCAAAGCTAA CTTCACAGTT CGCCACAACG 901 TTGAAGATGG TTCCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA AATTGGCGAT 961 GGCCCTGTCC CTTTTTACCA GACAACCCAT TACCCTGTCG ACACAAACTG TCCCTTTTCG 1021 AAAGATCCCA AACGGAAAAA GGTTAATTCT TAGAGGGCCC CGATTCAAGC CCGACTGGAT 1081 TCCAGTCCTC GAACCTTTGT GTGC // |
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Primer: BGH_rev2 Region: bGHpA,insert3'(GFP1-10,3XFLAG,Tom20(1-33)) Sequence file: RDB16041_A8I3b.seq
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>D05803_A5_A8I3_1_BGH_rev2_B08_05_ABI24.ab1 1 TTCATTTCAG TAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT 61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGATTAC TTTTCGTTGG GATCTTTCGA 121 AAGGACAGTT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC CATCGCCAAT 181 TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAA CCATCTTCAA CGTTGTGGCG 241 AACTGTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT ATACATTGTG 301 TGAGTTAAAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT TAAAATCAGT 361 ACCCTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTA CAGCACGCGT 421 CTTGTATTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC CTTCGGGCAT 481 GGCACTCTTG AAAAAGTCAT GCCTTTTCAT GTGATCCGGA TAACGGGAAA AGCATTGAAC 541 ACCATAGGTC AGAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC CAGTAGTGCA 601 AATAAATTTA AGTGTGAGTT TTCCGATTGT AGCATCACCT TCACCCTCTC CTCTGACAGA 661 AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA 721 AAGTTCTTCT CCTTTGCTCA TACTAGTGCC ACCGGCGGCC GCGCCAGAGC CGCCCTTATC 781 GTCATCGTCC TTGTAATCGA TGTCGTGATC CTTATAGTCG CCATCGTGGT CCTTGTAATC 841 ATTGGGGGTC GGATCTCCGC TTCCGGTCAA AATAGATACA GTAGCCGATG AACAGGGCGC 901 CGCACACGCC GGCGGCGATG GCGCTGTTCC TGCCCACCAT GGTGGCGGAT CCGAGCTCGG 961 TACCAAGCTT AAGTTTAAAC GCTAGCCCAG CTTTGGGGTC TCCCTATAGT TGAGTCGTAT 1021 TAATTTCGAT AGCCAGTTAG CAGTGAAATC TTCTAGTTAG CAGAGAAGCT CTGCCTATAT 1081 AGACCTCCCA ACCG // |
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Primer: SV40pro_F Region: SV40 pro_ori,NeoR Sequence file: RDB16041_A8I3c.seq
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>D05803_A5_A8I3_1_SV40pro_F_C08_08_ABI24.ab1 1 ACGGGTCCCT TGGCATCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG 961 CCCAACCTGC CATCACGAGA TTTTCGATTC CACCGCCGCC CTTCTATGAA AGGTTGGGGC 1021 TTCGGAATCG GTTTTCCGGG AACGCCCGGC TGGATGAATC GTTCAGCGGC GGGGGGATCT 1081 CATTGCATG // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. |
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2020.10.02
GNP_filter3_RDBDEP_html_200316.pl