Resource data sheet
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Tom20N-FLAG-GFP1-10 (#RDB16041)

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: 3xFLAG-GFP1-10; location: mitochondria.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kakimoto, Y., Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pMM80, pcDNA3.1_Tom20N-3xFLAG-GFP1-10, pcDNA3.1_Tom20N-FLAG-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 fused with3XFLAG-GFP1-10 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig. S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human TOMM20 cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

Catalog # Resource name Shipping form Fee
RDB16041 Tom20N-FLAG-GFP1-10 DNA solution

How to cite this biological resource

Materials & Methods section:

The Tom20N-FLAG-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16041).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [PubMed] [Article] [RRC of NBRP]