Please review the QC test results indicated by icon below as well as clone information before placing your order.

Tom20N-FLAG-GFP1-10

Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 for visualizing organelle contact sites in vivo.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering	Please visit Information of Request for Distribution.  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16041_A8I3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB16041_A8I3a.seq
>D05803_A5_A8I3_1_CMV-Forward_A08_02_ABI24.ab1     1 CTTCAACTTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG   121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG   181 GATCCGCCAC CATGGTGGGC AGGAACAGCG CCATCGCCGC CGGCGTGTGC GGCGCCCTGT   241 TCATCGGCTA CTGTATCTAT TTTGACCGGA AGCGGAGATC CGACCCCAAT GATTACAAGG   301 ACCACGATGG CGACTATAAG GATCACGACA TCGATTACAA GGACGATGAC GATAAGGGCG   361 GCTCTGGCGC GGCCGCCGGT GGCACTAGTA TGAGCAAAGG AGAAGAACTT TTCACTGGAG   421 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGAG   481 GAGAGGGTGA AGGTGATGCT ACAATCGGAA AACTCACACT TAAATTTATT TGCACTACTG   541 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTCT GACCTATGGT GTTCAATGCT   601 TTTCCCGTTA TCCGGATCAC ATGAAAAGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG   661 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAAATACAAG ACGCGTGCTG   721 TAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATCGTATCGA GTTAAAGGGT ACTGATTTTA   781 AAGAAGATGG AAACATTCTC GGACACAAAC TCGAGTACAA CTTTAACTCA CACAATGTAT   841 ACATCACGGC AGACAAACAA AAGAATGGAA TCAAAGCTAA CTTCACAGTT CGCCACAACG   901 TTGAAGATGG TTCCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA AATTGGCGAT   961 GGCCCTGTCC CTTTTTACCA GACAACCCAT TACCCTGTCG ACACAAACTG TCCCTTTTCG  1021 AAAGATCCCA AACGGAAAAA GGTTAATTCT TAGAGGGCCC CGATTCAAGC CCGACTGGAT 1081 TCCAGTCCTC GAACCTTTGT GTGC //
Primer: BGH_rev2 Sequence file: RDB16041_A8I3b.seq
>D05803_A5_A8I3_1_BGH_rev2_B08_05_ABI24.ab1     1 TTCATTTCAG TAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT    61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGATTAC TTTTCGTTGG GATCTTTCGA   121 AAGGACAGTT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC CATCGCCAAT   181 TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAA CCATCTTCAA CGTTGTGGCG   241 AACTGTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT ATACATTGTG   301 TGAGTTAAAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT TAAAATCAGT   361 ACCCTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTA CAGCACGCGT   421 CTTGTATTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC CTTCGGGCAT   481 GGCACTCTTG AAAAAGTCAT GCCTTTTCAT GTGATCCGGA TAACGGGAAA AGCATTGAAC   541 ACCATAGGTC AGAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC CAGTAGTGCA   601 AATAAATTTA AGTGTGAGTT TTCCGATTGT AGCATCACCT TCACCCTCTC CTCTGACAGA   661 AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA   721 AAGTTCTTCT CCTTTGCTCA TACTAGTGCC ACCGGCGGCC GCGCCAGAGC CGCCCTTATC   781 GTCATCGTCC TTGTAATCGA TGTCGTGATC CTTATAGTCG CCATCGTGGT CCTTGTAATC   841 ATTGGGGGTC GGATCTCCGC TTCCGGTCAA AATAGATACA GTAGCCGATG AACAGGGCGC   901 CGCACACGCC GGCGGCGATG GCGCTGTTCC TGCCCACCAT GGTGGCGGAT CCGAGCTCGG   961 TACCAAGCTT AAGTTTAAAC GCTAGCCCAG CTTTGGGGTC TCCCTATAGT TGAGTCGTAT  1021 TAATTTCGAT AGCCAGTTAG CAGTGAAATC TTCTAGTTAG CAGAGAAGCT CTGCCTATAT 1081 AGACCTCCCA ACCG //
Primer: SV40pro_F Sequence file: RDB16041_A8I3c.seq
>D05803_A5_A8I3_1_SV40pro_F_C08_08_ABI24.ab1     1 ACGGGTCCCT TGGCATCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA    61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG   121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG   661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT   781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG   961 CCCAACCTGC CATCACGAGA TTTTCGATTC CACCGCCGCC CTTCTATGAA AGGTTGGGGC  1021 TTCGGAATCG GTTTTCCGGG AACGCCCGGC TGGATGAATC GTTCAGCGGC GGGGGGATCT 1081 CATTGCATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl