Please review the QC test results indicated by icon below as well as clone information before placing your order.

Tom20N-FLAG-GFP1-10

Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 for visualizing organelle contact sites in vivo.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16041_A8I3p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,T7 pro,insert 5'(Tom20(1-33),3XFLAG,GFP1-10 5') Sequence file: RDB16041_A8I3d.seq
>D05803_A5_A8I3_1_CMV-Forward_A08_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NGCNNNGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA    61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG   121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG   181 GATCCGCCAC CATGGTGGGC AGGAACAGCG CCATCGCCGC CGGCGTGTGC GGCGCCCTGT   241 TCATCGGCTA CTGTATCTAT TTTGACCGGA AGCGGAGATC CGACCCCAAT GATTACAAGG   301 ACCACGATGG CGACTATAAG GATCACGACA TCGATTACAA GGACGATGAC GATAAGGGCG   361 GCTCTGGCGC GGCCGCCGGT GGCACTAGTA TGAGCAAAGG AGAAGAACTT TTCACTGGAG   421 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGAG   481 GAGAGGGTGA AGGTGATGCT ACAATCGGAA AACTCACACT TAAATTTATT TGCACTACTG   541 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTCT GACCTATGGT GTTCAATGCT   601 TTTCCCGTTA TCCGGATCAC ATGAAAAGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG   661 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAAATACAAG ACGCGTGCTG   721 TAGTCAAGTT TGAAGGTGAT ACCNTTGTTA ATCGTATCGA GTTAAAGGGT ACTGATTTTA   781 AAGAAGATGG AAACATTCTC GGACACAAAC TCGNNTACAA CTTTAACTCA CACAANGTAT   841 ACATCACGGC AGACAAACAA AAGANTGGAA TCNAAGCTAA CTTCACAGTT CGCCNCAACG 901 NNNAANATGG NNCCGTNCAN NNNNNNNNN //
Primer: BGH_rev2 Region: bGH pA,insert3'(GFP1-10) Sequence file: RDB16041_A8I3e.seq
>D05803_A5_A8I3_1_BGH_rev2_B08_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNGGNG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT    61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGATTAC TTTTCGTTGG GATCTTTCGA   121 AAGGACAGTT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC CATCGCCAAT   181 TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAA CCATCTTCAA CGTTGTGGCG   241 AACTGTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT ATACATTGTG   301 TGAGTTAAAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT TAAAATCAGT   361 ACCCTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTA CAGCACGCGT   421 CTTGTATTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC CTTCGGGCAT   481 GGCACTCTTG AAAAAGTCAT GCCTTTTCAT GTGATCCGGA TAACGGGAAA AGCATTGAAC   541 ACCATAGGTC AGAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC CAGTAGTGCA   601 AATAAATTTA AGTGTGAGTT TTCCGATTGT AGCATCACCT TCACCCTCTC CTCTGACAGA   661 AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA   721 AAGTTCTTCT CCTTTGCTCA TACTAGTGCC ACCGGCGGCC GCGCCAGANC CGCCCTTATC   781 GTCATCGTCC TTGTAATCGA TGTCGTGATC CTTATAGTCG CCATCGNGGN CCTTGTAATC   841 ATTGGGGGTC GGNTCNNNNC TNNCGNNCAA AATAGATACA GTAGCCNATG ANCAGGGNGC 901 CNCACACGCC NGCGGNNNNN GCGCTNNNCC NGNNNNCNNN NNNGNNNNNN NNNN //
Primer: SV40pro_F Region: NeoR Sequence file: RDB16041_A8I3f.seq
>D05803_A5_A8I3_1_SV40pro_F_C08_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNA NNNNTGNNGN NGCTTTTTTG GANGCCTAGG CTTTTGCNAA    61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG   121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG   661 CCCGACGGCG AGGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT   781 CANGNCATAG CGTTGGCTAC NCGNGNTATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAN   841 CGCTTCCTCG NGCTTTACGG TATCGCCGCN CCCNATTCGC AGCGCATCNC CNTCNANNGC 901 CTTCTNNNCN ANTNCTNCNN NNCGGGANTN NNGGGGNNCN NANTGANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl