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Tom20N-FLAG-GFP1-10

Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 for visualizing organelle contact sites in vivo.

Catalog number RDB16041
Resource name Tom20N-FLAG-GFP1-10
Alternative name pcDNA3.1_Tom20N-FLAG-GFP1-10 (pMM80)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 fused with3XFLAG-GFP1-10 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig. S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human TOMM20 cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank human TOMM20 (NCBI Gene 9804) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
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RDB16041 Tom20N-FLAG-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16041_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB16041_A8I3a.seq check
>D05803_A5_A8I3_1_CMV-Forward_A08_02_ABI24.ab1
    1 CTTCAACTTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG
  181 GATCCGCCAC CATGGTGGGC AGGAACAGCG CCATCGCCGC CGGCGTGTGC GGCGCCCTGT
  241 TCATCGGCTA CTGTATCTAT TTTGACCGGA AGCGGAGATC CGACCCCAAT GATTACAAGG
  301 ACCACGATGG CGACTATAAG GATCACGACA TCGATTACAA GGACGATGAC GATAAGGGCG
  361 GCTCTGGCGC GGCCGCCGGT GGCACTAGTA TGAGCAAAGG AGAAGAACTT TTCACTGGAG
  421 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGAG
  481 GAGAGGGTGA AGGTGATGCT ACAATCGGAA AACTCACACT TAAATTTATT TGCACTACTG
  541 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTCT GACCTATGGT GTTCAATGCT
  601 TTTCCCGTTA TCCGGATCAC ATGAAAAGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG
  661 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAAATACAAG ACGCGTGCTG
  721 TAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATCGTATCGA GTTAAAGGGT ACTGATTTTA
  781 AAGAAGATGG AAACATTCTC GGACACAAAC TCGAGTACAA CTTTAACTCA CACAATGTAT
  841 ACATCACGGC AGACAAACAA AAGAATGGAA TCAAAGCTAA CTTCACAGTT CGCCACAACG
  901 TTGAAGATGG TTCCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA AATTGGCGAT
  961 GGCCCTGTCC CTTTTTACCA GACAACCCAT TACCCTGTCG ACACAAACTG TCCCTTTTCG
 1021 AAAGATCCCA AACGGAAAAA GGTTAATTCT TAGAGGGCCC CGATTCAAGC CCGACTGGAT
1081 TCCAGTCCTC GAACCTTTGT GTGC
//
Primer: BGH_rev2
Sequence file: RDB16041_A8I3b.seq check
>D05803_A5_A8I3_1_BGH_rev2_B08_05_ABI24.ab1
    1 TTCATTTCAG TAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGATTAC TTTTCGTTGG GATCTTTCGA
  121 AAGGACAGTT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC CATCGCCAAT
  181 TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAA CCATCTTCAA CGTTGTGGCG
  241 AACTGTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT ATACATTGTG
  301 TGAGTTAAAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT TAAAATCAGT
  361 ACCCTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTA CAGCACGCGT
  421 CTTGTATTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC CTTCGGGCAT
  481 GGCACTCTTG AAAAAGTCAT GCCTTTTCAT GTGATCCGGA TAACGGGAAA AGCATTGAAC
  541 ACCATAGGTC AGAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC CAGTAGTGCA
  601 AATAAATTTA AGTGTGAGTT TTCCGATTGT AGCATCACCT TCACCCTCTC CTCTGACAGA
  661 AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA
  721 AAGTTCTTCT CCTTTGCTCA TACTAGTGCC ACCGGCGGCC GCGCCAGAGC CGCCCTTATC
  781 GTCATCGTCC TTGTAATCGA TGTCGTGATC CTTATAGTCG CCATCGTGGT CCTTGTAATC
  841 ATTGGGGGTC GGATCTCCGC TTCCGGTCAA AATAGATACA GTAGCCGATG AACAGGGCGC
  901 CGCACACGCC GGCGGCGATG GCGCTGTTCC TGCCCACCAT GGTGGCGGAT CCGAGCTCGG
  961 TACCAAGCTT AAGTTTAAAC GCTAGCCCAG CTTTGGGGTC TCCCTATAGT TGAGTCGTAT
 1021 TAATTTCGAT AGCCAGTTAG CAGTGAAATC TTCTAGTTAG CAGAGAAGCT CTGCCTATAT
1081 AGACCTCCCA ACCG
//
Primer: SV40pro_F
Sequence file: RDB16041_A8I3c.seq check
>D05803_A5_A8I3_1_SV40pro_F_C08_08_ABI24.ab1
    1 ACGGGTCCCT TGGCATCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG
  961 CCCAACCTGC CATCACGAGA TTTTCGATTC CACCGCCGCC CTTCTATGAA AGGTTGGGGC
 1021 TTCGGAATCG GTTTTCCGGG AACGCCCGGC TGGATGAATC GTTCAGCGGC GGGGGGATCT
1081 CATTGCATG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl