The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: 3xFLAG-GFP1-10; location: mitochondria.
Alternative name | pMM80, pcDNA3.1_Tom20N-3xFLAG-GFP1-10, pcDNA3.1_Tom20N-FLAG-GFP1-10 |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 fused with3XFLAG-GFP1-10 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig. S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pcDNA3.1 (plasmid) |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | human TOMM20 cDNA |
Depositor|Developer | Tamura, Yasushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16041 | Tom20N-FLAG-GFP1-10 | DNA solution |
Materials & Methods section:
The Tom20N-FLAG-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16041). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16041_A8I3p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro,T7 pro,insert 5'(Tom20(1-33),3XFLAG,GFP1-10 5') Sequence file: RDB16041_A8I3d.seq ![]() |
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>D05803_A5_A8I3_1_CMV-Forward_A08_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NGCNNNGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA 61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG 121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG 181 GATCCGCCAC CATGGTGGGC AGGAACAGCG CCATCGCCGC CGGCGTGTGC GGCGCCCTGT 241 TCATCGGCTA CTGTATCTAT TTTGACCGGA AGCGGAGATC CGACCCCAAT GATTACAAGG 301 ACCACGATGG CGACTATAAG GATCACGACA TCGATTACAA GGACGATGAC GATAAGGGCG 361 GCTCTGGCGC GGCCGCCGGT GGCACTAGTA TGAGCAAAGG AGAAGAACTT TTCACTGGAG 421 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGAG 481 GAGAGGGTGA AGGTGATGCT ACAATCGGAA AACTCACACT TAAATTTATT TGCACTACTG 541 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTCT GACCTATGGT GTTCAATGCT 601 TTTCCCGTTA TCCGGATCAC ATGAAAAGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG 661 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAAATACAAG ACGCGTGCTG 721 TAGTCAAGTT TGAAGGTGAT ACCNTTGTTA ATCGTATCGA GTTAAAGGGT ACTGATTTTA 781 AAGAAGATGG AAACATTCTC GGACACAAAC TCGNNTACAA CTTTAACTCA CACAANGTAT 841 ACATCACGGC AGACAAACAA AAGANTGGAA TCNAAGCTAA CTTCACAGTT CGCCNCAACG 901 NNNAANATGG NNCCGTNCAN NNNNNNNNN // |
Primer: BGH_rev2 (Pr0606) Region: bGH pA,insert3'(GFP1-10) Sequence file: RDB16041_A8I3e.seq ![]() |
>D05803_A5_A8I3_1_BGH_rev2_B08_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNGGNG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT 61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGATTAC TTTTCGTTGG GATCTTTCGA 121 AAGGACAGTT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC CATCGCCAAT 181 TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAA CCATCTTCAA CGTTGTGGCG 241 AACTGTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT ATACATTGTG 301 TGAGTTAAAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT TAAAATCAGT 361 ACCCTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTA CAGCACGCGT 421 CTTGTATTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC CTTCGGGCAT 481 GGCACTCTTG AAAAAGTCAT GCCTTTTCAT GTGATCCGGA TAACGGGAAA AGCATTGAAC 541 ACCATAGGTC AGAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC CAGTAGTGCA 601 AATAAATTTA AGTGTGAGTT TTCCGATTGT AGCATCACCT TCACCCTCTC CTCTGACAGA 661 AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA 721 AAGTTCTTCT CCTTTGCTCA TACTAGTGCC ACCGGCGGCC GCGCCAGANC CGCCCTTATC 781 GTCATCGTCC TTGTAATCGA TGTCGTGATC CTTATAGTCG CCATCGNGGN CCTTGTAATC 841 ATTGGGGGTC GGNTCNNNNC TNNCGNNCAA AATAGATACA GTAGCCNATG ANCAGGGNGC 901 CNCACACGCC NGCGGNNNNN GCGCTNNNCC NGNNNNCNNN NNNGNNNNNN NNNN // |
Primer: SV40pro_F (Pr0110) Region: NeoR Sequence file: RDB16041_A8I3f.seq ![]() |
>D05803_A5_A8I3_1_SV40pro_F_C08_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNA NNNNTGNNGN NGCTTTTTTG GANGCCTAGG CTTTTGCNAA 61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 661 CCCGACGGCG AGGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT 781 CANGNCATAG CGTTGGCTAC NCGNGNTATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAN 841 CGCTTCCTCG NGCTTTACGG TATCGCCGCN CCCNATTCGC AGCGCATCNC CNTCNANNGC 901 CTTCTNNNCN ANTNCTNCNN NNCGGGANTN NNGGGGNNCN NANTGANNNN NNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
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