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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Tom20N-FLAG-GFP1-10 (#RDB16041)

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: 3xFLAG-GFP1-10; location: mitochondria.

Alternative name pMM80, pcDNA3.1_Tom20N-3xFLAG-GFP1-10, pcDNA3.1_Tom20N-FLAG-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 fused with3XFLAG-GFP1-10 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig. S4 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human TOMM20 cDNA
Depositor|Developer Tamura, Yasushi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

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RDB16041 Tom20N-FLAG-GFP1-10 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Tom20N-FLAG-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16041).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16041_A8I3p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert 5'(Tom20(1-33),3XFLAG,GFP1-10 5')
Sequence file: RDB16041_A8I3d.seq check
>D05803_A5_A8I3_1_CMV-Forward_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NGCNNNGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG
  181 GATCCGCCAC CATGGTGGGC AGGAACAGCG CCATCGCCGC CGGCGTGTGC GGCGCCCTGT
  241 TCATCGGCTA CTGTATCTAT TTTGACCGGA AGCGGAGATC CGACCCCAAT GATTACAAGG
  301 ACCACGATGG CGACTATAAG GATCACGACA TCGATTACAA GGACGATGAC GATAAGGGCG
  361 GCTCTGGCGC GGCCGCCGGT GGCACTAGTA TGAGCAAAGG AGAAGAACTT TTCACTGGAG
  421 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGAG
  481 GAGAGGGTGA AGGTGATGCT ACAATCGGAA AACTCACACT TAAATTTATT TGCACTACTG
  541 GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTCT GACCTATGGT GTTCAATGCT
  601 TTTCCCGTTA TCCGGATCAC ATGAAAAGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG
  661 GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAAATACAAG ACGCGTGCTG
  721 TAGTCAAGTT TGAAGGTGAT ACCNTTGTTA ATCGTATCGA GTTAAAGGGT ACTGATTTTA
  781 AAGAAGATGG AAACATTCTC GGACACAAAC TCGNNTACAA CTTTAACTCA CACAANGTAT
  841 ACATCACGGC AGACAAACAA AAGANTGGAA TCNAAGCTAA CTTCACAGTT CGCCNCAACG
901 NNNAANATGG NNCCGTNCAN NNNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA,insert3'(GFP1-10)
Sequence file: RDB16041_A8I3e.seq check
>D05803_A5_A8I3_1_BGH_rev2_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGNG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGATTAC TTTTCGTTGG GATCTTTCGA
  121 AAGGACAGTT TGTGTCGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC CATCGCCAAT
  181 TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGGAA CCATCTTCAA CGTTGTGGCG
  241 AACTGTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCGTGATGT ATACATTGTG
  301 TGAGTTAAAG TTGTACTCGA GTTTGTGTCC GAGAATGTTT CCATCTTCTT TAAAATCAGT
  361 ACCCTTTAAC TCGATACGAT TAACAAGGGT ATCACCTTCA AACTTGACTA CAGCACGCGT
  421 CTTGTATTTC CCGTCATCTT TGAAAGATAT AGTGCGTTCC TGTACATAAC CTTCGGGCAT
  481 GGCACTCTTG AAAAAGTCAT GCCTTTTCAT GTGATCCGGA TAACGGGAAA AGCATTGAAC
  541 ACCATAGGTC AGAGTAGTGA CAAGTGTTGG CCATGGAACA GGTAGTTTTC CAGTAGTGCA
  601 AATAAATTTA AGTGTGAGTT TTCCGATTGT AGCATCACCT TCACCCTCTC CTCTGACAGA
  661 AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA
  721 AAGTTCTTCT CCTTTGCTCA TACTAGTGCC ACCGGCGGCC GCGCCAGANC CGCCCTTATC
  781 GTCATCGTCC TTGTAATCGA TGTCGTGATC CTTATAGTCG CCATCGNGGN CCTTGTAATC
  841 ATTGGGGGTC GGNTCNNNNC TNNCGNNCAA AATAGATACA GTAGCCNATG ANCAGGGNGC
901 CNCACACGCC NGCGGNNNNN GCGCTNNNCC NGNNNNCNNN NNNGNNNNNN NNNN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB16041_A8I3f.seq check
>D05803_A5_A8I3_1_SV40pro_F_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA NNNNTGNNGN NGCTTTTTTG GANGCCTAGG CTTTTGCNAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
  781 CANGNCATAG CGTTGGCTAC NCGNGNTATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAN
  841 CGCTTCCTCG NGCTTTACGG TATCGCCGCN CCCNATTCGC AGCGCATCNC CNTCNANNGC
901 CTTCTNNNCN ANTNCTNCNN NNCGGGANTN NNGGGGNNCN NANTGANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]