The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP11; location: endoplasmic reticulum.
Alternative name | pSFL15, pRS314-GPDp-YNR021W-GFP11 |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae endoplasmic reticulum (ER) protein YNR021W (hypothetical protein) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS314 (plasmid) |
Selectable markers | Amp^r (E. coli), TRP1 |
Gene/insert name | S. cerevisiae YNR021W cDNA |
Depositor|Developer | Tamura, Yasushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16040 | YNR021W-GFP11 | DNA solution |
Materials & Methods section:
The YNR021W-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16040). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16040_A8I3p1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 () Sequence file: RDB16040_A8I3a.seq ![]() |
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>D05803_A4_A8I3_1_M13_-40_D10_10_ABI24.ab1 1 AACAAGGCAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT 61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA 121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG 181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT 241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA 301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG 361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC 421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC 481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC 541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT 601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG TTAGTCTTTT 661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGT CTAGTTCAAT 721 ATTTGGCCCA CTTACGGGTT TTTTGGAGCG TGTCAATTCA CTCAATGCGC CCTACCAAGC 781 ATTATCATAT GACGAACAAA AGGCCATGAC TATTTGGCAA AGAGTCAAAT TCTACAACTG 841 GACTTTTGAA TTGTGTGCTC TAGGTGTGTT GTTCTTGGGT TTATGCTTTC TACAAATTTG 901 GTAACTCCGT TAATTTGAAA GCGCGGGAAA TCAGATTTTC CAATCTTTGC ATTCATTTTT 961 TGGGCCCAAC GACTTAAAGT TTCTCCCGTG TAGGGTTTCA AATATCAATG ATTCCCAAAA 1021 TCTTTACAGT GGCAACAACC AAATACCATG GCTCCTCCTT CCTTTTGCTT AACTGACTAG 1081 GATCCGCCCA ATCAAAGGAA GCCAATC // |
Primer: CYC1ter_R () Sequence file: RDB16040_A8I3b.seq ![]() |
>D05803_A4_A8I3_1_CYC1ter_R_D01_10_ABI24.ab1 1 ACTTTCTTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC 61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTAATC 121 CCAGCAGCAT TTACGTACTC ATGAAGGACC ATGTGGTCAC GACTTGTTGA TCCGCCACCA 181 GACCCTCCGG ATCCCTGAAA TCTTGTTCTT TGCTTGTTTT TGAGACGTCT TTCTCTTTTC 241 TCTTTCATTT TTTGATCTAC CTTTTCTTGT TGACCAGAGG CCTTCAATTG ACGTCTTTTG 301 GCTTTTTCCA ATTCTAATTT CTTCTCCTTT GCCAATTCCA ATTCAGTTTC TTTCATAAAC 361 TTTTTGATCT TGTTCAGTTC TTGCTGACGC AAGTTGGTAG TTCTTTTCAA GATGTCGTTG 421 GTAATGAATA AGTTTGGAGA CTGTTGGACT AAATCTTGAG TGAACCCATC GTAGATTTCT 481 ACGACTAAGG AAATAATTTG GTTTAACAAC TTCAATTCAT TCTCATTTTG GGGAACAGCG 541 CAACGAATGA TAGCCTTTGG TTCAATTGAA GATTCCCATT CTTTATCATC GCGTGGCGGG 601 TTGACAGGCA AATCGGTGAA ACTTATATAT TTCAAAAGAT TACCAGCTTG GGACAAAACC 661 TCATAGGGCT TGGAGTAATG AAACATGAAC CCTGATAATT GATTAACATC GGACATGTAG 721 ACGAAGTTAT TTGGTAGTTT ATCGTTTTCA CTAGTGTGTG CAATAGACAG GAAATAGTTT 781 TCAGTTCTAG CTTGATTCAT GAATTCTTTG TTAACAATAG AAGTGACAAA TCTAAATTTG 841 GTCAAAATTT CATGCGCATT CTTGTTAGGA TGAGCAGATT CACTAGTAAC TAAAATACCA 901 TTTGGCCTGA TCACGATCTC CATAAATTCT TCCAATTGCT TACTTTTTCA ACTTGCAAAA 961 AAAAAAAAAT CCAAAGCAGG TACTCTAGAC ACATAGAGAA AGGGTTTGCC TACCGGCCTA 1021 CCCAGAATGA TGGTTTAGGA TGAATGCTCT TGATGCGAAT CTACTGTAGC AAAAGGAAGA 1081 GGAACCA // |
Primer: TRP1pro_F () Sequence file: RDB16040_A8I3c.seq ![]() |
>D05803_A4_A8I3_1_TRP1pro_F_G02_20_ABI24.ab1 1 GCAGGGCATT AAGCAACAAG GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT 61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG 121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC 181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC 241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA 301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG 361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC 421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG 481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC 541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC 601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA 661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT 721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA 781 GATGCGTAAG GAGAAAATAC CGCATCAGGA AATTGTAAGC GTTAATATTT TGTTAAAATT 841 CGCGTTAAAT TTTTTGTTAA ATCAGCTCAT TTTTTTAACC AATAGGCCGA AATCGGCAAA 901 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAA 961 CAAGAGTCCC ACTATTAAAG AAACGGTGGA CTCCAAACGT CAAAGGCGAA AAACCCGGTC 1021 TATCATGGCG ATGGACCACT ACGTGACCAT CACCTATTCA GTTTTTTGGG GATCGAGGTG 1081 CCGTAAAAAG GCCAACCTA // |
Primer: Amp_R () Sequence file: RDB16040_A8I3d.seq ![]() |
>D05803_A4_A8I3_1_Amp_R_H02_23_ABI24.ab1 1 TCGTTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 121 TGGGTCCTTT TCATCACGTG CTATAAAAAT AATTATAATT TAAATTTTTT AATATAAATA 181 TATAAATTAA AAATAGAAAG TAAAAAAAGA AATTAAAGAA AAAATAGTTT TTGTTTTCCG 241 AAGATGTAAA AGACTCTAGG GGGATCGCCA ACAAATACTA CCTTTTATCT TGCTCTTCCT 301 GCTCTCAGGT ATTAATGCCG AATTGTTTCA TCTTGTCTGT GTAGAAGACC ACACACGAAA 361 ATCCTGTGAT TTTACATTTT ACTTATCGTT AATCGAATGT ATATCTATTT AATCTGCTTT 421 TCTTGTCTAA TAAATATATA TGTAAAGTAC GCTTTTTGTT GAAATTTTTT AAACCTTTGT 481 TTATTTTTTT TTCTTCATTC CGTAACTCTT CTACCTTCTT TATTTACTTT CTAAAATCCA 541 AATACAAAAC ATAAAAATAA ATAAACACAG AGTAAATTCC CAAATTATTC CATCATTAAA 601 AGATACGAGG CGCGTGTAAG TTACAGGCAA GCGATCCGTC TAAGAAACCA TTATTATCAT 661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA 721 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC 781 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG 841 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA GATCACGACA 901 TTACTATATA TATAATATAG GAAGCATTTA ATAGAACAGC ATCGTAAATA TATGTGTACT 961 TTGCAGTTAT GACGCCAGAT GGCAGTAGTG GAGATATTCT TTTATTGAAA AATAGCTTGG 1021 TCACCTTACG TACATCTTGA ATTCGAAGCC TTTTCCTTTT TTTGCCGATA GAATCAGTTC 1081 CGTTCGAAGA AG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
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