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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

YNR021W-GFP11

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP11; location: endoplasmic reticulum.

Catalog number RDB16040
Resource name YNR021W-GFP11
Alternative name pSFL15, pRS314-GPDp-YNR021W-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae endoplasmic reticulum (ER) protein YNR021W (hypothetical protein) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae YNR021W cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
S. cerevisiae YNR021W

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16040 YNR021W-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0090e (English text)
          Featured content Dnaconda's recommendation EXR0090j (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16040_A8I3p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB16040_A8I3a.seq check
          >D05803_A4_A8I3_1_M13_-40_D10_10_ABI24.ab1
              1 AACAAGGCAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT
             61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA
            121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG
            181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT
            241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA
            301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG
            361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC
            421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC
            481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC
            541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT
            601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG TTAGTCTTTT
            661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGT CTAGTTCAAT
            721 ATTTGGCCCA CTTACGGGTT TTTTGGAGCG TGTCAATTCA CTCAATGCGC CCTACCAAGC
            781 ATTATCATAT GACGAACAAA AGGCCATGAC TATTTGGCAA AGAGTCAAAT TCTACAACTG
            841 GACTTTTGAA TTGTGTGCTC TAGGTGTGTT GTTCTTGGGT TTATGCTTTC TACAAATTTG
            901 GTAACTCCGT TAATTTGAAA GCGCGGGAAA TCAGATTTTC CAATCTTTGC ATTCATTTTT
            961 TGGGCCCAAC GACTTAAAGT TTCTCCCGTG TAGGGTTTCA AATATCAATG ATTCCCAAAA
           1021 TCTTTACAGT GGCAACAACC AAATACCATG GCTCCTCCTT CCTTTTGCTT AACTGACTAG
          1081 GATCCGCCCA ATCAAAGGAA GCCAATC
          //
          Primer: CYC1ter_R
          Sequence file: RDB16040_A8I3b.seq check
          >D05803_A4_A8I3_1_CYC1ter_R_D01_10_ABI24.ab1
              1 ACTTTCTTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC
             61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTAATC
            121 CCAGCAGCAT TTACGTACTC ATGAAGGACC ATGTGGTCAC GACTTGTTGA TCCGCCACCA
            181 GACCCTCCGG ATCCCTGAAA TCTTGTTCTT TGCTTGTTTT TGAGACGTCT TTCTCTTTTC
            241 TCTTTCATTT TTTGATCTAC CTTTTCTTGT TGACCAGAGG CCTTCAATTG ACGTCTTTTG
            301 GCTTTTTCCA ATTCTAATTT CTTCTCCTTT GCCAATTCCA ATTCAGTTTC TTTCATAAAC
            361 TTTTTGATCT TGTTCAGTTC TTGCTGACGC AAGTTGGTAG TTCTTTTCAA GATGTCGTTG
            421 GTAATGAATA AGTTTGGAGA CTGTTGGACT AAATCTTGAG TGAACCCATC GTAGATTTCT
            481 ACGACTAAGG AAATAATTTG GTTTAACAAC TTCAATTCAT TCTCATTTTG GGGAACAGCG
            541 CAACGAATGA TAGCCTTTGG TTCAATTGAA GATTCCCATT CTTTATCATC GCGTGGCGGG
            601 TTGACAGGCA AATCGGTGAA ACTTATATAT TTCAAAAGAT TACCAGCTTG GGACAAAACC
            661 TCATAGGGCT TGGAGTAATG AAACATGAAC CCTGATAATT GATTAACATC GGACATGTAG
            721 ACGAAGTTAT TTGGTAGTTT ATCGTTTTCA CTAGTGTGTG CAATAGACAG GAAATAGTTT
            781 TCAGTTCTAG CTTGATTCAT GAATTCTTTG TTAACAATAG AAGTGACAAA TCTAAATTTG
            841 GTCAAAATTT CATGCGCATT CTTGTTAGGA TGAGCAGATT CACTAGTAAC TAAAATACCA
            901 TTTGGCCTGA TCACGATCTC CATAAATTCT TCCAATTGCT TACTTTTTCA ACTTGCAAAA
            961 AAAAAAAAAT CCAAAGCAGG TACTCTAGAC ACATAGAGAA AGGGTTTGCC TACCGGCCTA
           1021 CCCAGAATGA TGGTTTAGGA TGAATGCTCT TGATGCGAAT CTACTGTAGC AAAAGGAAGA
          1081 GGAACCA
          //
          Primer: TRP1pro_F
          Sequence file: RDB16040_A8I3c.seq check
          >D05803_A4_A8I3_1_TRP1pro_F_G02_20_ABI24.ab1
              1 GCAGGGCATT AAGCAACAAG GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT
             61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG
            121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC
            181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC
            241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA
            301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG
            361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC
            421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG
            481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC
            541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC
            601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA
            661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT
            721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA
            781 GATGCGTAAG GAGAAAATAC CGCATCAGGA AATTGTAAGC GTTAATATTT TGTTAAAATT
            841 CGCGTTAAAT TTTTTGTTAA ATCAGCTCAT TTTTTTAACC AATAGGCCGA AATCGGCAAA
            901 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAA
            961 CAAGAGTCCC ACTATTAAAG AAACGGTGGA CTCCAAACGT CAAAGGCGAA AAACCCGGTC
           1021 TATCATGGCG ATGGACCACT ACGTGACCAT CACCTATTCA GTTTTTTGGG GATCGAGGTG
          1081 CCGTAAAAAG GCCAACCTA
          //
          Primer: Amp_R
          Sequence file: RDB16040_A8I3d.seq check
          >D05803_A4_A8I3_1_Amp_R_H02_23_ABI24.ab1
              1 TCGTTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
             61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
            121 TGGGTCCTTT TCATCACGTG CTATAAAAAT AATTATAATT TAAATTTTTT AATATAAATA
            181 TATAAATTAA AAATAGAAAG TAAAAAAAGA AATTAAAGAA AAAATAGTTT TTGTTTTCCG
            241 AAGATGTAAA AGACTCTAGG GGGATCGCCA ACAAATACTA CCTTTTATCT TGCTCTTCCT
            301 GCTCTCAGGT ATTAATGCCG AATTGTTTCA TCTTGTCTGT GTAGAAGACC ACACACGAAA
            361 ATCCTGTGAT TTTACATTTT ACTTATCGTT AATCGAATGT ATATCTATTT AATCTGCTTT
            421 TCTTGTCTAA TAAATATATA TGTAAAGTAC GCTTTTTGTT GAAATTTTTT AAACCTTTGT
            481 TTATTTTTTT TTCTTCATTC CGTAACTCTT CTACCTTCTT TATTTACTTT CTAAAATCCA
            541 AATACAAAAC ATAAAAATAA ATAAACACAG AGTAAATTCC CAAATTATTC CATCATTAAA
            601 AGATACGAGG CGCGTGTAAG TTACAGGCAA GCGATCCGTC TAAGAAACCA TTATTATCAT
            661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA
            721 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC
            781 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG
            841 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA GATCACGACA
            901 TTACTATATA TATAATATAG GAAGCATTTA ATAGAACAGC ATCGTAAATA TATGTGTACT
            961 TTGCAGTTAT GACGCCAGAT GGCAGTAGTG GAGATATTCT TTTATTGAAA AATAGCTTGG
           1021 TCACCTTACG TACATCTTGA ATTCGAAGCC TTTTCCTTTT TTTGCCGATA GAATCAGTTC
          1081 CGTTCGAAGA AG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.08.11

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