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YNR021W-GFP11

Expression vector of S. cerevisiae endoplasmic reticulum (ER) protein YNR021W (hypothetical protein) for visualizing organelle contact sites in vivo.

Catalog number RDB16040
Resource name YNR021W-GFP11
Alternative name pRS314-GPDp-YNR021W-GFP11 (pSFL15)
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae endoplasmic reticulum (ER) protein YNR021W (hypothetical protein) fused with GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae YNR021W cDNA
Depositor Tamura, Yasushi |
 
Other clones in our bank S. cerevisiae YNR021W |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB16040 YNR021W-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0090j (Japanese text)
Featured content Dnaconda's recommendation EXR0090e (English text)
Featured content Sensor and Visualization (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16040_A8I3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB16040_A8I3a.seq check
>D05803_A4_A8I3_1_M13_-40_D10_10_ABI24.ab1
    1 AACAAGGCAT GTATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT CATTATCAAT
   61 ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA
  121 GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG
  181 TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT
  241 AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA
  301 AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG
  361 AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC
  421 CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC
  481 ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC
  541 AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT
  601 TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG TTAGTCTTTT
  661 TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCATGT CTAGTTCAAT
  721 ATTTGGCCCA CTTACGGGTT TTTTGGAGCG TGTCAATTCA CTCAATGCGC CCTACCAAGC
  781 ATTATCATAT GACGAACAAA AGGCCATGAC TATTTGGCAA AGAGTCAAAT TCTACAACTG
  841 GACTTTTGAA TTGTGTGCTC TAGGTGTGTT GTTCTTGGGT TTATGCTTTC TACAAATTTG
  901 GTAACTCCGT TAATTTGAAA GCGCGGGAAA TCAGATTTTC CAATCTTTGC ATTCATTTTT
  961 TGGGCCCAAC GACTTAAAGT TTCTCCCGTG TAGGGTTTCA AATATCAATG ATTCCCAAAA
 1021 TCTTTACAGT GGCAACAACC AAATACCATG GCTCCTCCTT CCTTTTGCTT AACTGACTAG
1081 GATCCGCCCA ATCAAAGGAA GCCAATC
//
Primer: CYC1ter_R
Sequence file: RDB16040_A8I3b.seq check
>D05803_A4_A8I3_1_CYC1ter_R_D01_10_ABI24.ab1
    1 ACTTTCTTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC GTGACATAAC
   61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTAATC
  121 CCAGCAGCAT TTACGTACTC ATGAAGGACC ATGTGGTCAC GACTTGTTGA TCCGCCACCA
  181 GACCCTCCGG ATCCCTGAAA TCTTGTTCTT TGCTTGTTTT TGAGACGTCT TTCTCTTTTC
  241 TCTTTCATTT TTTGATCTAC CTTTTCTTGT TGACCAGAGG CCTTCAATTG ACGTCTTTTG
  301 GCTTTTTCCA ATTCTAATTT CTTCTCCTTT GCCAATTCCA ATTCAGTTTC TTTCATAAAC
  361 TTTTTGATCT TGTTCAGTTC TTGCTGACGC AAGTTGGTAG TTCTTTTCAA GATGTCGTTG
  421 GTAATGAATA AGTTTGGAGA CTGTTGGACT AAATCTTGAG TGAACCCATC GTAGATTTCT
  481 ACGACTAAGG AAATAATTTG GTTTAACAAC TTCAATTCAT TCTCATTTTG GGGAACAGCG
  541 CAACGAATGA TAGCCTTTGG TTCAATTGAA GATTCCCATT CTTTATCATC GCGTGGCGGG
  601 TTGACAGGCA AATCGGTGAA ACTTATATAT TTCAAAAGAT TACCAGCTTG GGACAAAACC
  661 TCATAGGGCT TGGAGTAATG AAACATGAAC CCTGATAATT GATTAACATC GGACATGTAG
  721 ACGAAGTTAT TTGGTAGTTT ATCGTTTTCA CTAGTGTGTG CAATAGACAG GAAATAGTTT
  781 TCAGTTCTAG CTTGATTCAT GAATTCTTTG TTAACAATAG AAGTGACAAA TCTAAATTTG
  841 GTCAAAATTT CATGCGCATT CTTGTTAGGA TGAGCAGATT CACTAGTAAC TAAAATACCA
  901 TTTGGCCTGA TCACGATCTC CATAAATTCT TCCAATTGCT TACTTTTTCA ACTTGCAAAA
  961 AAAAAAAAAT CCAAAGCAGG TACTCTAGAC ACATAGAGAA AGGGTTTGCC TACCGGCCTA
 1021 CCCAGAATGA TGGTTTAGGA TGAATGCTCT TGATGCGAAT CTACTGTAGC AAAAGGAAGA
1081 GGAACCA
//
Primer: TRP1pro_F
Sequence file: RDB16040_A8I3c.seq check
>D05803_A4_A8I3_1_TRP1pro_F_G02_20_ABI24.ab1
    1 GCAGGGCATT AAGCAACAAG GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT
   61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG
  121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC
  181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC
  241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA
  301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG
  361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC
  421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG
  481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC
  541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC
  601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA
  661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT
  721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA
  781 GATGCGTAAG GAGAAAATAC CGCATCAGGA AATTGTAAGC GTTAATATTT TGTTAAAATT
  841 CGCGTTAAAT TTTTTGTTAA ATCAGCTCAT TTTTTTAACC AATAGGCCGA AATCGGCAAA
  901 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAA
  961 CAAGAGTCCC ACTATTAAAG AAACGGTGGA CTCCAAACGT CAAAGGCGAA AAACCCGGTC
 1021 TATCATGGCG ATGGACCACT ACGTGACCAT CACCTATTCA GTTTTTTGGG GATCGAGGTG
1081 CCGTAAAAAG GCCAACCTA
//
Primer: Amp_R
Sequence file: RDB16040_A8I3d.seq check
>D05803_A4_A8I3_1_Amp_R_H02_23_ABI24.ab1
    1 TCGTTTCATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
   61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
  121 TGGGTCCTTT TCATCACGTG CTATAAAAAT AATTATAATT TAAATTTTTT AATATAAATA
  181 TATAAATTAA AAATAGAAAG TAAAAAAAGA AATTAAAGAA AAAATAGTTT TTGTTTTCCG
  241 AAGATGTAAA AGACTCTAGG GGGATCGCCA ACAAATACTA CCTTTTATCT TGCTCTTCCT
  301 GCTCTCAGGT ATTAATGCCG AATTGTTTCA TCTTGTCTGT GTAGAAGACC ACACACGAAA
  361 ATCCTGTGAT TTTACATTTT ACTTATCGTT AATCGAATGT ATATCTATTT AATCTGCTTT
  421 TCTTGTCTAA TAAATATATA TGTAAAGTAC GCTTTTTGTT GAAATTTTTT AAACCTTTGT
  481 TTATTTTTTT TTCTTCATTC CGTAACTCTT CTACCTTCTT TATTTACTTT CTAAAATCCA
  541 AATACAAAAC ATAAAAATAA ATAAACACAG AGTAAATTCC CAAATTATTC CATCATTAAA
  601 AGATACGAGG CGCGTGTAAG TTACAGGCAA GCGATCCGTC TAAGAAACCA TTATTATCAT
  661 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA
  721 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC
  781 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG
  841 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA GATCACGACA
  901 TTACTATATA TATAATATAG GAAGCATTTA ATAGAACAGC ATCGTAAATA TATGTGTACT
  961 TTGCAGTTAT GACGCCAGAT GGCAGTAGTG GAGATATTCT TTTATTGAAA AATAGCTTGG
 1021 TCACCTTACG TACATCTTGA ATTCGAAGCC TTTTCCTTTT TTTGCCGATA GAATCAGTTC
1081 CGTTCGAAGA AG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.01.22

GNP_filter3_RDBDEP_html_191130.pl