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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pT7-3xFLAG-NLS-Flpe-polyA plasmid (#RDB16011)

FLAG-NLS-Flpe-myc expressing plasmid.

Clone info. FLAG-NLS-Flpe-myc expressing plasmid. Applicable for direct transfection into mammalian cells (CMV promoter) and in-vitro transcriotion (T7 promoter).
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Saccharomyces cerevisiae Flpe cDNA
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
 
Sequence (full) RDB16011zzt02.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Osawa, Y. et al., Elife 10: e59759 (2021).).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osawa, Y. et al., Elife 10: e59759 (2021).)。

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RDB16011 pT7-3xFLAG-NLS-Flpe-polyA plasmid DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pT7-3xFLAG-NLS-Flpe-polyA plasmid was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16011).

Reference section:

Osawa, Y., Murata, K., Usui, M., Kuba, Y., Le, H.T., Mikami, N., Nakagawa, T., Daitoku, Y., Kato, K., Shawki, H.H., Ikeda, Y., Kuno, A., Morimoto, K., Tanimoto, Y., Dinh, T.T.H., Yagami, K.I., Ema, M., Yoshida, S., Takahashi, S., Mizuno, S., Sugiyama, F., EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Elife 10: e59759 (2021). PMID 33973520. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16011_B2Cpp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(3XFLAG,SV40 NLS,Fipe 5')
Sequence file: RDB16011_B2Cpa.seq check
>16011_16011_B2Cp_3_CMV_Forward_E12_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGCGGTAG GCGTGNTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT
  121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC GGACTCTAGT AATACGACTC
  181 ACTATAGGGA GAGCCGCCAC CATGGACTAT AAGGACCACG ACGGAGACTA CAAGGATCAT
  241 GATATTGATT ACAAAGACGA TGACGATAAG GCCCCAAAGA AGAAGCGGAA GGTCGGTATC
  301 CACGGAGTCC CAGCAGCCAT GAGCCAATTT GATATATTAT GTAAAACACC ACCTAAGGTC
  361 CTGGTTCGTC AGTTTGTGGA AAGGTTTGAA AGACCTTCAG GGGAAAAAAT AGCATCATGT
  421 GCTGCTGAAC TAACCTATTT ATGTTGGATG ATTACTCATA ACGGAACAGC AATCAAGAGA
  481 GCCACATTCA TGAGCTATAA TACTATCATA AGCAATTCGC TGAGTTTCGA TATTGTCAAC
  541 AAATCACTCC NGTTTAAATA CAAGACGCAA AAAGCAACAA TTCTGGAAGC CTCATTAAAG
  601 AAATTAATTC CTGCTTGGGA ATTTACNATT ATTCCTTACA ATGGACAAAA NCATCAATCT
  661 GATATCACTG ATATTGNAAG TAGTTTGCNA NTACNGTTCG NATCATCNGN NGANNCANNN
721 NNNNNNAANN AGCCNCANNN NAAAAANANN NNNNNNNN
//
Primer: HSV-pA_R (Pr0949)
Region: insert 3'(Myc,Flpe 3')
Sequence file: RDB16011_B2Cpb.seq check
>16011_16011_B2Cp_3_HSV-pA_R_F12_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN NNTAGCCTCC CCCGTTTAAA CTCATTACTA
   61 ACCGGTAGGG ATCGAACCCT TTCACAGATC TTCTTCAGAA ATAAGTTTTT GTTCCACCGG
  121 TCCTATGCGT CTATTTATGT AGGATGAAAG GTAGTCTAGT ACCTCCTGTG ATATTATCCC
  181 ATTCCATGCG GGGTATCGTA TGCTTCCTTC AGCACTACCC TTTAGCTGTT CTATATGCTG
  241 CCACTCCTCA ATTGGATTAG TCTCATCCTT CAATGCTATC ATTTCCTTTG ATATTGGATC
  301 ATATGCATAG TACCGAGAAA CTAGTGCGAA GTAGTGATCA GGTATTGCTG TTATCTGATG
  361 AGTATACGTT GTCCTGGCCA CGGCAGAAGC ACGCTTATCG CTCCAATTTC CCACAACATT
  421 AGTCAACTCC GTTAGGCCCT TCATTGACAG AAATGAGGTC ATCAAATGTC TTCCAATGTG
  481 AGATTTTGGG CCATTCTTTA TAGCAAAGAT TGGATAAGGC GCATTTTTCT TCAAAGCCTT
  541 GTTGTACGAT CTGACTAAGT TATCTTTTAA TAATTGGTAT TCCTGTTTGT TGCTTGAAGA
  601 ATTGCCGGTC CTATTTACTC GTTTTANGAN TGGTTCANAG TTCCTCNAAA ATTCNTCCAA
  661 ATATACAAGT GGANCGNTCC TACCCCTTGC GCTNANNAAN TATATGTGCN NACNNACGCT
721 NNNCTTNNNC TCTGNNNNNN NANNNNNNNN NN
//
Primer: f1ori_F (Pr0280)
Region: f1 ori
Sequence file: RDB16011_B2Cpc.seq check
>16011_16011_B2Cp_3_f1ori_F_G12_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGTCNCGCTG CGCNNNNNNA CCACACCCGC
   61 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTG GGGATACCCC CTAGAGCCCC AGCTGCGCAG
  121 ATCTGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA
  181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT
  241 CGGTGGGGTA TCGATGTACA GCTAGCTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
301 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTNN NNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB16011_B2Cpd.seq check
>16011_16011_B2Cp_3_SV40pro_ori_F_H12_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNGC CTCTGCCTCT GNNCTATTCC NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
  121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
  181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
  241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
  301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
  361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
  421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
  481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
  541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
  601 GGTCTTGTCG ATCAGGANGA TCTGGACGAA NAGCATCANG GGCTCGCGCC AGCCGAACTG
  661 TTCGCCNGGC TCNAGGCGCG CNTGCCCNAN GGCGANGNNC TCGTCNNGNN CCNTNGNNNN
721 GNCNNGCNNN NCNANNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Osawa, Y., EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Elife 10: e59759 (2021). PMID 33973520. [link to RRC of NBRP]