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pT7-3xFLAG-NLS-Flpe-polyA (#RDB16011)

FLAG-NLS-Flpe-myc expressing plasmid.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Osawa, Y., Elife 10: e59759 (2021). PMID 33973520. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. FLAG-NLS-Flpe-myc expressing plasmid. Applicable for direct transfection into mammalian cells (CMV promoter) and in-vitro transcriotion (T7 promoter).
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name Saccharomyces cerevisiae Flpe cDNA
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Osawa, Y. et al., Elife 10: e59759 (2021).).
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必要書類
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osawa, Y. et al., Elife 10: e59759 (2021).)。

Catalog # Resource name Shipping form Fee
RDB16011 pT7-3xFLAG-NLS-Flpe-polyA DNA solution

How to cite this biological resource

Materials & Methods section:

The pT7-3xFLAG-NLS-Flpe-polyA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16011).

Reference section:

Osawa, Y., Murata, K., Usui, M., Kuba, Y., Le, H.T., Mikami, N., Nakagawa, T., Daitoku, Y., Kato, K., Shawki, H.H., Ikeda, Y., Kuno, A., Morimoto, K., Tanimoto, Y., Dinh, T.T.H., Yagami, K.I., Ema, M., Yoshida, S., Takahashi, S., Mizuno, S., Sugiyama, F., EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Elife 10: e59759 (2021). PMID 33973520. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Osawa, Y., EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Elife 10: e59759 (2021). PMID 33973520. [PubMed] [Article] [RRC of NBRP]
user_report Kurasawa, S., Hearing loss occurs prior to thrombocytopenia in both mice and humans with DFNA1. FASEB J. 39 (2): e70309 (2025). PMID 39831886. [PubMed] [Article] [RRC of NBRP]