Please review the QC test results indicated by icon below as well as clone information before placing your order.

pT7-3xFLAG-NLS-Flpe-polyA plasmid

FLAG-NLS-Flpe-myc expressing plasmid.

Sequence (full)	RDB16011zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Osawa, Y. et al., Elife 10: e59759 (2021).).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osawa, Y. et al., Elife 10: e59759 (2021).)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16011_B2Cpp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,insert 5'(3XFLAG,SV40 NLS,Fipe 5') Sequence file: RDB16011_B2Cpa.seq
>16011_16011_B2Cp_3_CMV_Forward_E12_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGCGGTAG GCGTGNTACG GTGGGAGGTC    61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT   121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC GGACTCTAGT AATACGACTC   181 ACTATAGGGA GAGCCGCCAC CATGGACTAT AAGGACCACG ACGGAGACTA CAAGGATCAT   241 GATATTGATT ACAAAGACGA TGACGATAAG GCCCCAAAGA AGAAGCGGAA GGTCGGTATC   301 CACGGAGTCC CAGCAGCCAT GAGCCAATTT GATATATTAT GTAAAACACC ACCTAAGGTC   361 CTGGTTCGTC AGTTTGTGGA AAGGTTTGAA AGACCTTCAG GGGAAAAAAT AGCATCATGT   421 GCTGCTGAAC TAACCTATTT ATGTTGGATG ATTACTCATA ACGGAACAGC AATCAAGAGA   481 GCCACATTCA TGAGCTATAA TACTATCATA AGCAATTCGC TGAGTTTCGA TATTGTCAAC   541 AAATCACTCC NGTTTAAATA CAAGACGCAA AAAGCAACAA TTCTGGAAGC CTCATTAAAG   601 AAATTAATTC CTGCTTGGGA ATTTACNATT ATTCCTTACA ATGGACAAAA NCATCAATCT   661 GATATCACTG ATATTGNAAG TAGTTTGCNA NTACNGTTCG NATCATCNGN NGANNCANNN 721 NNNNNNAANN AGCCNCANNN NAAAAANANN NNNNNNNN //
Primer: HSV-pA_R Region: insert 3'(Myc,Flpe 3') Sequence file: RDB16011_B2Cpb.seq
>16011_16011_B2Cp_3_HSV-pA_R_F12_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN NNTAGCCTCC CCCGTTTAAA CTCATTACTA    61 ACCGGTAGGG ATCGAACCCT TTCACAGATC TTCTTCAGAA ATAAGTTTTT GTTCCACCGG   121 TCCTATGCGT CTATTTATGT AGGATGAAAG GTAGTCTAGT ACCTCCTGTG ATATTATCCC   181 ATTCCATGCG GGGTATCGTA TGCTTCCTTC AGCACTACCC TTTAGCTGTT CTATATGCTG   241 CCACTCCTCA ATTGGATTAG TCTCATCCTT CAATGCTATC ATTTCCTTTG ATATTGGATC   301 ATATGCATAG TACCGAGAAA CTAGTGCGAA GTAGTGATCA GGTATTGCTG TTATCTGATG   361 AGTATACGTT GTCCTGGCCA CGGCAGAAGC ACGCTTATCG CTCCAATTTC CCACAACATT   421 AGTCAACTCC GTTAGGCCCT TCATTGACAG AAATGAGGTC ATCAAATGTC TTCCAATGTG   481 AGATTTTGGG CCATTCTTTA TAGCAAAGAT TGGATAAGGC GCATTTTTCT TCAAAGCCTT   541 GTTGTACGAT CTGACTAAGT TATCTTTTAA TAATTGGTAT TCCTGTTTGT TGCTTGAAGA   601 ATTGCCGGTC CTATTTACTC GTTTTANGAN TGGTTCANAG TTCCTCNAAA ATTCNTCCAA   661 ATATACAAGT GGANCGNTCC TACCCCTTGC GCTNANNAAN TATATGTGCN NACNNACGCT 721 NNNCTTNNNC TCTGNNNNNN NANNNNNNNN NN //
Primer: f1ori_F Region: f1 ori Sequence file: RDB16011_B2Cpc.seq
>16011_16011_B2Cp_3_f1ori_F_G12_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGTCNCGCTG CGCNNNNNNA CCACACCCGC    61 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTG GGGATACCCC CTAGAGCCCC AGCTGCGCAG   121 ATCTGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA   181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT   241 CGGTGGGGTA TCGATGTACA GCTAGCTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 301 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTNN NNNNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori,NeoR Sequence file: RDB16011_B2Cpd.seq
>16011_16011_B2Cp_3_SV40pro_ori_F_H12_08_ABI08.ab1     1 NNNNNNNNNN NNNNNGNNGC CTCTGCCTCT GNNCTATTCC NGAAGTAGTG AGGAGGCTTT    61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT   121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT   181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC   241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG   301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG   361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC   421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC   481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC   541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC   601 GGTCTTGTCG ATCAGGANGA TCTGGACGAA NAGCATCANG GGCTCGCGCC AGCCGAACTG   661 TTCGCCNGGC TCNAGGCGCG CNTGCCCNAN GGCGANGNNC TCGTCNNGNN CCNTNGNNNN 721 GNCNNGCNNN NCNANNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.29

GNP_filter3_RDBDEP_html_220812.pl