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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHisGST CKI homolog 1 (#RDB15965)

Bacterial expression vector of S. cerevisiae CKI homolog 1, residues 62-355.

Clone info. Bacterial expression vector of S. cerevisiae CKI homolog 1, residues 62-355, fused with fused with His-GST at N terminus.
Comment Expression was confirmed by the depositor.
Vector backbone pET-28a(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name S. cerevisiae CKI homolog 1 cDNA
Depositor|Developer Ueda, Hiroki |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shinohara, Y et al. Molecular Cell 67, 783–798, 2017)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shinohara, Y et al. Molecular Cell 67, 783–798, 2017)。

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RDB15965 pHisGST CKI homolog 1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHisGST CKI homolog 1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15965).

Reference section:

Shinohara, Y., Koyama, Y.M., Ukai-Tadenuma, M., Hirokawa, T., Kikuchi, M., Yamada, R.G., Ukai, H., Fujishima, H., Umehara, T., Tainaka, K., Ueda, H.R., Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock. Mol. Cell 67 (5): 783-798.e20 (2017). PMID 28886336. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15965_A8Ehp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: GST-M_F
Sequence file: RDB15965_A8Eha.seq check
>D05351A2_A8Eh_2_GST-M_F_D08_11_ABI24.ab1
    1 GCCGGTGGGA CGTTTGAAGC TCGTCAAGTT GATTTTCTTA GCGAAGCTAC CTGAAATGCT
   61 GAAAATGTTC GAAGATCGTT TATGTCATAA AACATATTTA AATGGTGATC ATGTAACCCA
  121 TCCTGACTTC ATGTTGTATG ACGCTCTTGA TGTTGTTTTA TACATGGACC CAATGTGCCT
  181 GGATGCGTTC CCAAAATTAG TTTGTTTTAA AAAACGTATT GAAGCTATCC CACAAATTGA
  241 TAAGTACTTG AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT
  301 TGGTGGTGGC GACCATCCTC CAAAATCGGA TGGGTCTAGC GGCTCCAGCG AACACCTGTA
  361 TTTTCAGGGA TCCACTATTG TCGGCCTACA TTACAAGATC GGCAAAAAAA TAGGGGAAGG
  421 TTCCTTTGGT GTGCTATTTG AAGGTACTAA TATGATCAAT GGCGTACCCG TCGCGATCAA
  481 ATTCGAGCCC AGAAAAACGG AGGCCCCTCA ATTAAGAGAT GAATATAAAA CATATAAAAT
  541 TCTGAATGGC ACTCCCAATA TCCCCTACGC GTACTACTTC GGCCAAGAAG GTTTGCACAA
  601 TATCTTGGTC ATTGATCTTT TGGGTCCCTC TTTGGAAGAT TTATTTGATT GGTGTGGAAG
  661 AAAATTTTCT GTCAAAACGG TTGTGCAAGT TGCTGTCCAA ATGATTACTT TGATTGAAGA
  721 CTTGCACGCA CATGACTTGA TATACCGTGA TATCAAACCA GACAATTTCT TGATTGGAAG
  781 GCCCGGCCAA CCTGACGCAA ACAACATCCA TTTGATCGAC TTCGGTATGG CCAAACAGTA
  841 TCGTGATCCG AAAACTAAAC AGCACATCCC ATATAGAGAG AAAAAAATCA CTCAGCGGCA
  901 CTGCCAGATA TATGTCCATT AATACTCACC TTGGAAGAGA GCAGTCCAGA AGGAGATGAT
  961 ATGGAGGGCC CTTGGGGTCA CGTTTTTCTT TTATTTCTTG AGAGGGCCCA CTTACCCTGG
 1021 CAGGATTTAA AAAGCTCCCA AACCATAAAG CCAAAAAATA CGAATAAGAC TGGCTGCACA
1081 GGCAAAGATC CTACTTAATC GTTTTTTA
//
Primer: f1ori_R3
Sequence file: RDB15965_A8Ehb.seq check
>D05351A2_A8Eh_2_f1ori-R3_E08_14_ABI24.ab1
    1 AGCATCCCAT CGGATTAGTT CCTCCTTTCA GCAAAACCCC TCAAGACCCG TTTAGAGGCC
   61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG
  121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGAGTTA CAGTTTCATC
  181 CAATCATATT GGCCGTCCGC GGTTTCACCT AAATCATCCA GTACAGATAG TAATAGTTTT
  241 CTATAGCCTT CATAATCGGG ACACTCTTCA AAGGAAAGAC TTCTGACGAT TTCTAGATAC
  301 CTGCCAAATT GCACAGGTAA GCCTTGAGCT AGATCGTAAA CGTTAGTAGA TCTTTTCTTT
  361 TCACCAATCT TTTCGTATTT TTGCTTATTG TTTGGAGCTT TTAAACCCTG CCAGGGTAAG
  421 TGGCCTCTCA AGAAATAAAA GAAAACGTGA CCCAAGGCCT CCATATCATC TCTTCTGGAC
  481 TGCTCTCTTC CAAGGTGAGT ATTAATGGAC ATATATCTGG CAGTGCCGCT GAGTGATTTT
  541 TTCTCTCTAT ATGGGATGTG CTGTTTAGTT TTCGGATCAC GATACTGTTT GGCCATACCG
  601 AAGTCGATCA AATGGATGTT GTTTGCGTCA GGTTGGCCGG GCCTTCCAAT CAAGAAATTG
  661 TCTGGTTTGA TATCACGGTA TATCAAGTCA TGTGCGTGCA AGTCTTCAAT CAAAGTAATC
  721 ATTTGGACAG CAACTTGCAC AACCGTTTTG ACAGAAAATT TTCTTCCACA CCAATCAAAT
  781 AAATCTTCCA AAGAGGGACC CAAAAGATCA ATGACCAAGA TATTGTGCAA ACCTTCTTGG
  841 CCGAAGTAGT ACGCGTAGGG GATATTGGGA GTGCCATTCA GAATTTTATA TGTTTTATAT
  901 TCATCTCTTA ATTTGAGGGC CTCCGTTTTT CTGGGCTCGA AATTTGATCG CGACGGGTAC
  961 GCCATTGATC ATATTAGTAC CTTCAAATAG CACACCCAAG GAACCTTTCC CCTATTTTTT
 1021 TTTGCCCGAT CTGTTAATGT AGCCCGACAT AGTGGCATCC CTGAAATACA GTGATCGCTG
1081 GGAGCCGCTA GACCCATCCC GGAAGT
//
Primer: GST-N_rev
Sequence file: RDB15965_A8Ehc.seq check
>D05351A2_A8Eh_2_GST-N_rev_F08_17_ABI24.ab1
    1 TATCCTTCAG GATTTTTCCA AAAGAAGTCG GAGTGGGTTG CACAAGGCCC TTAATTTTCC
   61 AATAACCTAG TATAGGGGAC ATGGCATGAG CGTGCTCATG TTTGTGATGA TTGTGGATGA
  121 GATGATCTTT GCCCATGGTA TATCTCCTTC TTAAAGTTAA ACAAAATTAT TTCTAGAGGG
  181 GAATTGTTAT CCGCTCACAA TTCCCCTATA GTGAGTCGTA TTAATTTCGC GGGATCGAGA
  241 TCTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG
  301 CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA
  361 TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG
  421 CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC
  481 TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAGATCCC GGACACCATC
  541 GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG
  601 GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT
  661 CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA
  721 GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG
  781 GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG
  841 CAAATTGTCG CGGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG TGGTGGTGTC
  901 GATGGTAGAA CGAAGCGGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTTCTCGCG
  961 CAACGCGTCA GTGGGGCTGA TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTTGCCT
 1021 GTGGAAAGCT GCCCTGCAAC TAATGTTTCC GGCGTTTATT CCTTGGAATG TCTCCTGACC
1081 CAGACACTCC ATTCCACCAG GTAAATTTAA TTTTTTCCTT GCCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shinohara, Y., Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock. Mol. Cell 67 (5): 783-798.e20 (2017). PMID 28886336. [link to RRC of NBRP]