Expression vector of mCherry and ble under the control of Perkinsus marinus MOE gene promoter.
Clone info. | Bicistronic expression vector of mCherry and Sh-ble intervened by a self-cleaving 2A peptide, which is controled under the Perkinsus marinus MOE (PmMOE) promoter. This clone is for a drug selection system using bleomycin for transfecting Perkinsus species. Perkinsus is sensitive to bleomycin, and 100 microgram/ml of this drug completely blocks its proliferation. |
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Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pCR4-TOPO (Plasmid) |
Size of vector backbone | 4 kb |
Selectable markers | Amp^r, Kan^r. |
Gene/insert name | Perkinsus marinus MOE genomic DNA Discoma sp. RFP cDNA Streptoalloteichus hindustanus bleomycin resistance gene genomic DNA |
Depositor|Developer | Nozaki, Tomoyoshi | Matsuzaki, Motomichi | |
Sequence | RDB15791z.seq |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press). |
必要書類 | 提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定するいずれかの文献を引用する (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15791 | pMRB | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pMRB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15791). |
Reference section:
Sakamoto, H., Kita, K., Matsuzaki, M., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J. Eukaryot. Microbiol. 68 (5): e12861 (2021). PMID 34051022. [link to RRC of NBRP] |
Sakamoto, H., Hirakawa, Y., Ishida, K., Keeling, P.J., Kita, K., Matsuzaki, M., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Sakamoto, H., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J. Eukaryot. Microbiol. 68 (5): e12861 (2021). PMID 34051022. [link to RRC of NBRP] |
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original | Sakamoto, H., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616. [link to RRC of NBRP] |
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