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pMRB (#RDB15791)

Expression vector of mCherry and ble under the control of Perkinsus marinus MOE gene promoter.

Clone info. Bicistronic expression vector of mCherry and Sh-ble intervened by a self-cleaving 2A peptide, which is controled under the Perkinsus marinus MOE (PmMOE) promoter.
This clone is for a drug selection system using bleomycin for transfecting Perkinsus species. Perkinsus is sensitive to bleomycin, and 100 microgram/ml of this drug completely blocks its proliferation.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pCR4-TOPO (Plasmid)
Size of vector backbone 4 kb
Selectable markers Amp^r, Kan^r.
Gene/insert name Perkinsus marinus MOE genomic DNA Discoma sp. RFP cDNA Streptoalloteichus hindustanus bleomycin resistance gene genomic DNA
Depositor|Developer Nozaki, Tomoyoshi | Matsuzaki, Motomichi |
 
Sequence RDB15791z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定するいずれかの文献を引用する (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press)。

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RDB15791 pMRB Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMRB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15791).

Reference section:

Sakamoto, H., Kita, K., Matsuzaki, M., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J. Eukaryot. Microbiol. 68 (5): e12861 (2021). PMID 34051022. [link to RRC of NBRP]
Sakamoto, H., Hirakawa, Y., Ishida, K., Keeling, P.J., Kita, K., Matsuzaki, M., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Sakamoto, H., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J. Eukaryot. Microbiol. 68 (5): e12861 (2021). PMID 34051022. [link to RRC of NBRP]
original Sakamoto, H., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616. [link to RRC of NBRP]