RDB15791 - pMRB

pMRB

Expression vector of mCherry and ble under the control of Perkinsus marinus MOE gene promoter.

Catalog number	RDB15791
Resource name	pMRB
Clone info.	Bicistronic expression vector of mCherry and Sh-ble intervened by a self-cleaving 2A peptide, which is controled under the Perkinsus marinus MOE (PmMOE) promoter. This clone is for a drug selection system using bleomycin for transfecting Perkinsus species. Perkinsus is sensitive to bleomycin, and 100 microgram/ml of this drug completely blocks its proliferation.
Comment	Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone	pCR4-TOPO (Plasmid)
Size of vector backbone	4 kb
Selectable markers	Amp^r, Kan^r.
Gene/insert name	Perkinsus marinus MOE genomic DNA Discoma sp. RFP cDNA Streptoalloteichus hindustanus bleomycin resistance gene genomic DNA
Depositor\|Developer	Nozaki, Tomoyoshi \| Matsuzaki, Motomichi \|

Other clones in our bank

External Database
Perkinsus marinus MOE

Discoma sp. RFP

Streptoalloteichus hindustanus bleomycin resistance gene

Reference sequence

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press).

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This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).

Remarks
This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press).
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定するいずれかの文献を引用する (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15791	pMRB	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Electronic file

Sequence	RDB15791z.seq

Original reference

original	Sakamoto, H., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J Eukaryot Microbiol . 2021 May 29;e12861. doi: 10.1111/jeu.12861. Online ahead of print. PMID 34051022.
original	Sakamoto, H., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Original, user report and related articles

original	Sakamoto, H., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J Eukaryot Microbiol . 2021 May 29;e12861. doi: 10.1111/jeu.12861. Online ahead of print. PMID 34051022.
original	Sakamoto, H., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616.

2022.05.17

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