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pMGP

Expression vector of EGFP and pac under the control of Perkinsus marinus MOE gene promoter.

Catalog number RDB15790
Resource name pMGP
Clone info. Bicistronic expression vector of EGFP and pac intervened by a self-cleaving 2A peptide, which is controled under the Perkinsus marinus MOE (PmMOE) promoter.
This clone is for a drug selection system using puromycin for transfecting Perkinsus species. Perkinsus is sensitive to puromycin, and 100 microgram/ml of this drug completely blocks its proliferation.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pCR4-TOPO (Plasmid)
Size of vector backbone 4 kb
Selectable markers Amp^r, Kan^r.
Gene/insert name Perkinsus marinus MOE genomic DNA Aequorea victoria GFP cDNA Streptomyces alboniger puromycin-N-acetyl-transferase (pac) genomic DNA
Depositor|Developer Nozaki, Tomoyoshi | Matsuzaki, Motomichi |
Other clones in our bank

External Database
Perkinsus marinus MOE
    Aequorea victoria GFP
      Streptomyces alboniger puromycin-N-acetyl-transferase (pac)

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press).
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定するいずれかの文献を引用する (Sakamoto, H. et al., Parasitol. Int. 69: 13-16 (2018); Sakamoto, H. et al., J. Eukaryot. Microbiol. in press)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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            RDB15790 pMGP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB15790z.seq

            Original reference

            original Sakamoto, H., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J Eukaryot Microbiol
            . 2021 May 29;e12861. doi: 10.1111/jeu.12861. Online ahead of print. PMID 34051022.
            original Sakamoto, H., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616.

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15790_A8E8p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: SV40pA3' element Reverse
            Sequence file: RDB15790_A8E8a.seq check
            >15790_15790_A8E8_2_SV40pA3'ElementReverse_F02_17_ABI24.ab1
                1 TGGGGGGGGG GGCGGGAAAC CTATGGGGAA AAACGCCAGC AACGCGGGCC TTTTTACGGT
               61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
              121 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
              181 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC
              241 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG
              301 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC
              361 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG
              421 GAAACAGCTA TGACCATGAT TACGCCAAGC TCAGAATTAA CCCTCACTAA AGGGACTAGT
              481 CCTGCAGGTT TAAACGAATT CGCCCTTCTC GTAATGAGCC CAACCATTAT CGGATCGGTC
              541 TTGCGGGCGA ACGAACATCT GCGGCAGGTG AGAGGTGGGA GTTTCCGGAT GCGGTCCGAG
              601 TCATCGGCGA TGGAACTCCC CACAGGCCAG TGTGACGGTG AGCACTGATG TTCGATGGAG
              661 ACCAAAGTGG CAGGGTTCGT ACTTCCCAGC CATGGTTGCG CCGTTTGTAA TTGATGTGAT
              721 GGCAGAGCTG ACCGGTAGCA GTAGAAGGTG CGTTGATTCA GCTCAACAGC TCTCTTGTGT
              781 TAGCAGGAAA AGTAGAAGCA GATCTAGGAT GATTCAGGAA ATCCCCTCAT CTTAGCGGTA
              841 GTATGTTACT GTGCACCCAT CATGCCCATG GATGATCCGG GATCCCTTGT TGGGGTGAGG
              901 TAGGTCCCTT TGGTGAGTTG GGATGATTGT GGGATCTGCC GATGGGCAGA AGATCAATGG
              961 GCTCCATTCC CCCATCGGGG ACCCTCACGG GTAATCACTG AATCATTTTC AATGGGCTCA
             1021 AAGGGGCGGG GCTTTGGTCG ACTTTCGACA AGTGGGGGTG GGGGACTGCG GTGAGCAAAG
             1081 GGTGCCTTTG GCCACTTGGC CGAACCCAAT CGCTTTTGGC TTCCGGGCTT GTAATTGAAC
             1141 CTAACGGGGA AGCCAGGAAA TTAGGGCCCG GGGGGCTATA AAATGGGGGA GACGGCAAAC
            1201 TCACATCCAG
            //
            Primer: M13_-40
            Sequence file: RDB15790_A8E8b.seq check
            >15790_15790_A8E8_2_M13(-40)_G02_20_ABI24.ab1
                1 ACCCAAGGGA AATGGTATAC GACTCACTAT AGGGCGAATT GAATTTAGCG GCCGCGAATT
               61 CGCCCTTGGA GGACTTGAGG CTCTGTGACT ATACTCTTGA GATGAGGAAG ACTATGGTCT
              121 TCTTACAGAA ACTCAGCCGT GGCATGATAG CGGAGTAAAA GCCTTCATGT TGGTTTCCGA
              181 CCATGTTGTT ATCCATAGTC ACAACAAGCG TCGGAATAAC CATGGGATGC TCCTGGACGC
              241 GCTGAGGGGG TTGAACCTTC TGATCAATCG TGGTAAGCTT AGGAGGATCC GCACTGTTTA
              301 ATCATCTTCG CAGGGGCCAG TCTCCGAGTT GGAAGGGCCC AAGCAGCGGT GATCGCTGAA
              361 TGCAAGAAGG CAATCAAGAA CAAGGATGCG GCTACCCTAG TTGGTGAGTC AAGTATGACT
              421 GCCGTCGGCC ATTCCGGGTC TGCAGGTGTG ATCTCGCAGG GGGGCAATCT TGGTCCATCA
              481 GCATGATGTT GATGTGCTTA TCTTTCATCA TCATTATCAT TGATTATGTG TGATAGTCTC
              541 AATCATTAGA GTGCACTTCT TTGAGGGTAC TCGAGCCGAC CGAGTACACA CCTCTTTTCC
              601 GTCAAAAAAT TACAATGGAT TGCGCCTGCT AACTATGACG GAGAGAAGAC TCAACTATAA
              661 ACGTCTGTGG ATATCTACAT TCTGAGGACC AGTGGGCATC CGGGCAGGGT CAGTCCGGAG
              721 GTATGTTAGG CAGGTCAGCC TCCTTGTAAA TCAATGAAGG ATTTGTGTTA TCGGCTGCTG
              781 CTATTCCAAG CGCAGCTCAG TACGGAAATT ACGCGAGGAT GAAACAAAGT TGGGTTCATC
              841 GAGCTGGTGA AATGAGAGTA ATGGTGTTTT CTGAAATGGG TAGATACACA GGTTGTTATA
              901 TACAATTGGC CACTGGCACT CTCCCAAACG CAAACAAGAA CAAATGGAAG CGAGAACTTC
              961 TCTGTAGTAT CGTTCACGGT TACTTGAATC CCTAACATGG CACAGATTCC CGAAGGAGAG
             1021 AGGCCTTCTT ATACGTATTC TCCGACATGA TGACTCGTTA ACTCGGAGAT ACAAACCAAG
            1081 G
            //
            Primer: moe3_055R
            Sequence file: RDB15790_A8E8c.seq check
            >15790_15790_A8E8_2_moe3_055R_A03_03_ABI24.ab1
                1 CAAGGGTTGA ATTCCGAACA TTGATGTAGA TCTCAACTAG ATGTGTTGTC TTAGGCACCG
               61 GGCTTGCGGG TCATGCACCA GGTGCGCGGT CCTTCGGGCA CCTCGACGTC GGCGGTGACG
              121 GTGAAGCCGA GCCGCTCGTA GAAGGGGAGG TTGCGGGGCG CGGAGGTCTC CAGGAAGGCG
              181 GGCACCCCGG CGCGCTCGGC CGCCTCCACT CCGGGGAGCA CGACGGCGCT GCCCAGACCC
              241 TTGCCCTGGT GGTCGGGCGA GACGCCGACG GTGGCCAGGA ACCACGCGGG CTCCTTGGGC
              301 CGGTGCGGCG CCAGGAGGCC TTCCATCTGT TGCTGCGCGG CCAGCCGGGA ACCGCTCAAC
              361 TCGGCCATGC GCGGGCCGAT CTCGGCGAAC ACCGCCCCCG CTTCGACGCT CTCCGGCGTG
              421 GTCCAGACCG CCACCGCGGC GCCGTCGTCC GCGACCCACA CCTTGCCGAT GTCGAGCCCG
              481 ACGCGCGTGA GGAAGAGTTC TTGCAGCTCG GTGACCCGCT CGATGTGGCG GTCCGGGTCG
              541 ACGGTGTGGC GCGTGGCGGG GTAGTCGGCG AACGCGGCGG CGAGGGTGCG TACGGCCCGG
              601 GGGACGTCGT CGCGGGTGGC GAGGCGCACC GTGGGCTTGT ACTCGGTCAT AGGACCGGGG
              661 TTTTCTTCCA CGTCTCCTGC TTGCTTTAAC AGAGAGAAGT TCGTGGCTCC GCTTCCCTTG
              721 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG
              781 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCTTGGACT GGGTGCTCAG GTAGTGGTTG
              841 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC
              901 TGCACGCTGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TCACCTTGAT GCCGTTCTTC
              961 TGCTTGTCGG CCATGATATA GACGTTGTGG GCTGTTGTAG TTGTACTCCA GCTTTGTGCC
             1021 CCAGGATGTT TGCCGTCCTC CCTTGAAAGT CGATGCCCTT TCAGCTCGAT GCGATTCACA
             1081 AGGGGTGTCG CCCCTCGACT CAACCTTCGG CGCGGATCTT GTAGTTGGCC GGTTCGATCC
            1141 TGTGAG
            //
            Primer: EGFP-C-rev
            Sequence file: RDB15790_A8E8d.seq check
            >15790_15790_A8E8_2_EGFP-C_rev_H02_23_ABI24.ab1
                1 ACTCTGGATT GGGGTCTTTG CTCAGCTTGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA
               61 GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC
              121 TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT
              181 CGGCCATGAT ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT
              241 TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT
              301 CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT
              361 CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG
              421 GGTAGCGGCT GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA
              481 CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC
              541 CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA
              601 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACTCCGGAT CCGAGCTCCA
              661 TATGCACTTT GTGATTCATG ATTGCGTTGA ACAGCGGGAT AGAGATTCAA GAGTGACGCG
              721 AGTTCATCAA GAGTTCCACA CAACTCACTT AGCTATGTGT ATGCGTTCAA TGCGTCCGGT
              781 CGATAGACAT CGTTATAGCG TCTCGCCTTA CAACATAGAG TCCATTTAGA AGGATTCAGC
              841 GTTACGAAAT CAAGTGACTC GAGATTATGA TTTCCAAGCT AGTGCGAGAT TCGTGGAGAA
              901 ATGCCCTTTG ATGGACAAGA CTGTGTTGCT CCGCGATACA TACTCACGCA TACAATGAAA
              961 GGGAGCGTGA AGGGATATCT GTTGATGTCT ACTAGCAGAA CCCGTCCCTC CGCTCCTAAG
             1021 TTCTCTAGCT CGCTGACGCC TCAAGGGTTC TAGTGAAGAT TTCGACTCGA GGATGCACAT
             1081 CGTGGGAAGC ACTGCTTCGG AACGAGACCG AAATCCGAAC ATAAAAATTC TTAATGGAAT
            1141 TTTGG
            //
            Primer: Neo-M_R
            Sequence file: RDB15790_A8E8e.seq check
            >15790_15790_A8E8_2_Neo-M_R_B03_06_ABI24.ab1
                1 CTCTCCAATT GCAGCCAGTC CCTTCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG
               61 GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG
              121 CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA
              181 CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA
              241 CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC
              301 ATCCTGTCTC TTGATCAGAG CTTGATCCCC TGCGCCATCA GATCCTTGGC GGCAAGAAAG
              361 CCATCCAGTT TACTTTGCAG GGCTTCCCAA CCTTACCAGA GGGCGCCCCA GCTGGCAATT
              421 CCGGTTCGCT TGCTGTCCAT AAAACCGCCC AGTCTAGCTA TCGCCATGTA AGCCCACTGC
              481 AAGCTACCTG CTTTCTCTTT GCGCTTGCGT TTTCCCTTGT CCAGATAGCC CAGTAGCTGA
              541 CATTCATCCG GGGTCAGCAC CGTTTCTGCG GACTGGCTTT CTACGTGAAA AGGATCTAGG
              601 TGAAGATCCT TTTTGATAAT CTCATGCCTG ACATTTATAT TCCCCAGAAC ATCAGGTTAA
              661 TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC CACTTCTTCC CCGATAACGG
              721 AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA GCTTTCATCC CCGATATGCA
              781 CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG ACGTGCACTG GCCAGGGGGA
              841 TCACCATCCG TCGCCCCGGC GTGTCAATAA TATCACTCTG TACATCCACA AACAGACGAT
              901 AACGGCTCTC TCTTTTATAG GTGTAAACCT TAAACTGCCG TACGTATAGG CTGCGCAACT
              961 GTTGGGAAAG GGCGATCGGT GCGGGCCTCT TTCGCTATTA CGCCAGCTGG GCGAAGGGGG
             1021 ATGTGCTGCA AAGGCGATTT AAGTTGGGTA ACGCCCAGGG GTTTTTCCCA GTCACGACGT
             1081 TGTAAAACGA CGGCCAGTGG ATGTAATTAC GAACTCCACT ATAAGGGCGG AATTGGAATT
            1141 TTAAGCCGGG TG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Sakamoto, H., A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes. J Eukaryot Microbiol
            . 2021 May 29;e12861. doi: 10.1111/jeu.12861. Online ahead of print. PMID 34051022.
            original Sakamoto, H., Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol. Int. 69: 13-16 (2018). PMID 30389616.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl