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pmCherry2-hGRalpha/C421G-A458T

Expression vector of human GR alpha1, DNA-binding-deficient mutant and homodimerization-deficient mutant.

Catalog number RDB15755
Resource name pmCherry2-hGRalpha/C421G-A458T
Clone info. Expression vector of human glucocorticoid receptor (GR alpha1), DNA-binding-deficient mutant [C421G] and homodimerization-deficient mutant [A458T], fused with tandem mCherry at N-terminal, CMV promoter, see Fig. S1 of Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017.
Comment Expression was confirmed by the depositor with fluorescence cross-correlation spectroscopy (FCCS) and microscopic observation.
Vector backbone plasmid
Gene/insert name human NR3C1 cDNA
Depositor|Developer Kinjo, Masataka |
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External Database
human NR3C1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017)。
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          RDB15755 pmCherry2-hGRalpha/C421G-A458T Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pmCherry2-hGRalpha/C421G-A458T was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15755).

          Reference section:

          Tiwari, M., Oasa, S., Yamamoto, J., Mikuni, S., Kinjo, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Tiwari, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]

          2023.12.13

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