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pEGFP-C1-mouse Rab20(R59L)

Expression vector of mouse Rab20 constitutive active mutant EGFP-tag.

Catalog number RDB15732
Resource name pEGFP-C1-mouse Rab20(R59L)
Clone info. Expression vector of mouse Rab20 constitutive active mutant (GTP-hydrolysis-deficient, R59L), fused with EGFP at N-terminal. CMV promoter.
Expression was confirmed by the depositor with fluorescence microscope.Itoh, T. et al., Genes. Cells, 11 (9): 1023-1037, 2006 (PMID 16923123) for gene cloning.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab20 cDNA
Reference of nucleotide sequence AB232614 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab20 (NCBI Gene 19332) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)
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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15732 pEGFP-C1-mouse Rab20(R59L) Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

2019.07.17

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