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pEGFP-C1-mouse Rab20(R59L)

Expression vector of mouse Rab20 constitutive active mutant EGFP-tag.

Catalog number RDB15732
Resource name pEGFP-C1-mouse Rab20(R59L)
Clone info. Expression vector of mouse Rab20 constitutive active mutant (GTP-hydrolysis-deficient, R59L), fused with EGFP at N-terminal. CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.Itoh, T. et al., Genes. Cells, 11 (9): 1023-1037, 2006 (PMID 16923123) for gene cloning.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab20 cDNA
Reference of insert sequence AB232614 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab20

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15732 pEGFP-C1-mouse Rab20(R59L) Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

          2022.07.13

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