RDB15725 - pEF-HA-SNAP23-S95A/S120A

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pEF-HA-SNAP23-S95A/S120A

Expression vector of mouse synaptosomal-associated protein 23 (SNAP23), phosphorylation-deficient mutant (PD-SNAP23).


Catalog number	RDB15725
Resource name	pEF-HA-SNAP23-S95A/S120A
Clone info.	Expression vector of mouse synaptosomal-associated protein 23 (SNAP23), S95A/S120A mutant [phosphorylation-deficient mutant (PD-SNAP23)], tagged with HA at N-terminal. EF1 promoter. Expression was confirmed by the depositor with immunostaining. Please refer Fukuda, M. et al., J. Biol. Chem. 280 (47): 39175-39184, 2005 (PMID 16186111) for gene cloning.
Vector backbone	pEF-HA (plasmid)
Selectable markers	Amp^r (E. coli).
Gene/insert name	mouse Snap23 cDNA
Depositor	Fukuda, Mitsunori \|
Other clones in our bank	mouse Snap23 (NCBI Gene 20619) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (fukuda, M. et al. J. Biol. Chem. 280, 39175-39184, 2005); (Klein, O et al. Sci. Rep. 7, 15315, 2017)

Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

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Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (fukuda, M. et al. J. Biol. Chem. 280, 39175-39184, 2005); (Klein, O et al. Sci. Rep. 7, 15315, 2017)
Additional terms and conditions for distribution	Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(fukuda, M. et al. J. Biol. Chem. 280, 39175-39184, 2005); (Klein, O. et al. Sci. Rep. 7, 15315, 2017)。
付加的提供条件	pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15725	pEF-HA-SNAP23-S95A/S120A	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original	Klein, O., Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis. Sci. Rep. 7 (1): 15315 (2017). PMID 29127297.

Further references such as user reports and related articles (go to bottom)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Klein, O., Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis. Sci. Rep. 7 (1): 15315 (2017). PMID 29127297.
reference	Fukuda, M., Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner. J. Biol. Chem. 280 (47): 39175-39184 (2005). PMID 16186111.

2019.12.10

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