Expression vector of mouse synaptosomal-associated protein 23 (SNAP23), phosphorylation-deficient mutant (PD-SNAP23).
Catalog number | RDB15725 |
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Resource name | pEF-HA-SNAP23-S95A/S120A |
Clone info. | Expression vector of mouse synaptosomal-associated protein 23 (SNAP23), S95A/S120A mutant [phosphorylation-deficient mutant (PD-SNAP23)], tagged with HA at N-terminal. EF1 promoter. Expression was confirmed by the depositor with immunostaining. Please refer Fukuda, M. et al., J. Biol. Chem. 280 (47): 39175-39184, 2005 (PMID 16186111) for gene cloning. |
Vector backbone | pEF-HA (plasmid) |
Selectable markers | Amp^r (E. coli). |
Gene/insert name | mouse Snap23 cDNA |
Depositor | Fukuda, Mitsunori | |
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Other clones in our bank | mouse Snap23 (NCBI Gene 20619) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (fukuda, M. et al. J. Biol. Chem. 280, 39175-39184, 2005); (Klein, O et al. Sci. Rep. 7, 15315, 2017) |
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Additional terms and conditions for distribution | Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15725 | pEF-HA-SNAP23-S95A/S120A | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
original | Klein, O., Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis. Sci. Rep. 7 (1): 15315 (2017). PMID 29127297. |
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Further references such as user reports and related articles (go to bottom)
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Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Klein, O., Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis. Sci. Rep. 7 (1): 15315 (2017). PMID 29127297. |
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reference | Fukuda, M., Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner. J. Biol. Chem. 280 (47): 39175-39184 (2005). PMID 16186111. |
2019.12.10
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