Expression vector of human SH2-containing inositol phosphatase-2 (SHIP2), fused with N-terminal GFP.
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External Database Human INPPL1 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukumoto, M et al. Cancer Sci. 108(5): 941-951, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Kobe University. |
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Ordering | Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukumoto, M et al. Cancer Sci. 108(5): 941-951, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請及び事業活動を行う場合は、寄託者と別途協議を行う。非営利機関が非営利目的の教育·研究用に用いる場合以外は、神戸大学と別途MTAを締結する。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15719 | pEGFP-C1-SHIP2 | DNA solution |
Original reference
original | Fukumoto, M., PI(3,4)P2 plays critical roles in the regulation of focal adhesion dynamics of MDA-MB-231 breast cancer cells. Cancer Sci. 108 (5): 941-951 (2017). PMID 28247964. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15719_A8A9p1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EGFP-C (Pr0025) Region: EGFP 3',insert 5'(SHIP2) Sequence file: RDB15719_A8A9a.seq |
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>D05009A1-1_A8A9_EGFP-C_A01_01_ABI24.ab1 1 ACGGGAATAT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCTC GACCCGGGTC 61 AGGATCCTCA GAATTCACCA TGGCCTCGGC CTGCGGGGCG CCGGGCCCGG GGGGCGCCCT 121 GGGCAGCCAG GCCCCCTCCT GGTACCACCG CGACCTGAGC CGGGCGGCCG CGGAGGAGCT 181 GCTGGCCCGG GCGGGCCGCG ATGGCAGCTT CCTGGTCCGA GACAGCGAGA GCGTGGCGGG 241 GGCCTTCGCG CTCTGCGTCC TGTATCAGAA GCATGTGCAC ACGTATCGCA TTCTGCCTGA 301 TGGAGAAGAT TTCTTGGCTG TGCAGACCTC GCAGGGTGTG CCTGTGCGCC GCTTCCAGAC 361 CCTGGGTGAG CTCATCGGCC TGTACGCCCA GCCCAACCAG GGCCTTGTGT GCGCCCTGCT 421 TCTTCCTGTA GAGGGTGAGC GAGAGCCGGA CCCACCGGAT GACCGGGATG CCTCAGATGG 481 GGAGGATGAG AAGCCCCCGC TGCCCCCGCG CTCTGGCTCC ACCAGCATTT CTGCCCCCAC 541 TGGGCCCAGC AGTCCCCTGC CAGCTCCTGA GACTCCCACA GCTCCAGCTG CTGAGAGTGC 601 TCCCAATGGG CTGAGCACCG TCTCGCACGA CTACCTGAAA GGCAGCTATG GGCTGGACCT 661 GGAAGCTGTG AGGGGTGGAG CCAGCCACCT GCCCCACCTC ACCCGTACCC TCGCTACCTC 721 ATGCCGGAGG CTGCACAGTG AGGTGGACAA GGTCCTGTCA GGCCTGGAGA TCCTGTCCAA 781 GGTGTTTGAC CAGCAGAGCT CGCCCATGGT GACCCGCCTT TTGCAGCAGC AGAACCTGCC 841 ACAGACAGGG GAGCAGGAAC TAGAGAGCCT GGTGCTGAAG CTGTCAGTGC TAAAGGACTT 901 CCTGTCAGGC ATCCAGAAGA AGGCCCTGAA GGCCCTACAG GACATGAGCT CCACAGCACC 961 CCCAGCTCCG CAGCCATCCA CACGTAAAGG CCAAGACCAT CCCCGTGCAG GCCTTTGAGG 1021 TGAAGCTAGA TGTGACCCTG GGTGACCTGA CCAGATTTGG GAAGTCGCAG AAGTTTCACG 1081 CTGAGCGTGC ATGTGGAGGG GTGGGCCGCT GGTTGCCTGC CTGCCGGAGA ACAGCGGGAC 1141 CTTCCCCAGG GAGGAACCTG TGGA // |
Primer: SV40polyA-R (Pr0109) Region: insert 3' Sequence file: RDB15719_A8A9b.seq |
>D05009A1-1_A8A9_SV40polyA-R_B01_04_ABI24.ab1 1 AAAATTTGGA GTTTTAAAGC AAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT 61 CAGTTATCTA GATCCGGTGG ATCAGTCGAC CTTGCTGAGC TGCAGGGTGT CCAGAAGGAG 121 GCGCTTGTGA GCCGGGTCCT GCACCCCAGC CTCCTCCAAG TCCTCCTCGG TGATGTCACT 181 GAGAAACTCC AGGTCGTCCC AGCCATTATG CACCAGGCCC TCCTCATAGC GCTCCAAGCC 241 GATGGCCCGC AGCCAGGCAC TCATGCCTGC CTCGCCCAGC CCGCTGGCCC GCCCGCCCTG 301 CAGGCACGGA GCCTCCTCTG CCAGGTCTTC CTGGATGCTC TCCCGGATGC GGGGTGGAGG 361 GAAGCTGAGG GGCCGGCCAT AGTCCGAGGG CAGTCCCCGG GGGGGCTGCA GCTCCAGGGG 421 GCCTGGGAGG AGCGCTGAGC GTGCAGGCCC AGCAGGGGCA TAGTCCACCT CGCTCAGCGT 481 CTTGGCCATC TGCAGCACCG AGCAGGACCG GTCATCCCCA CTGGCCACTT CCCCCAGGAA 541 AGTGCTGGCT GGTGAGGGGC CTGGGGGCAG TGGAGGCCTT GGATGGGCCT TGGGAGGTGG 601 TGGGCCACGG GCCTCACCCC CACCACGGCC CCGGACCACT GGCCCTGGTA AAGGATCCAG 661 GCTGGGGGGC AGGAAGATGG CTGAGTCCGG CAGTGGTGGA GGTGGAAAGT CTGGAGGGGG 721 CAGTGTGCCT CCAGACTCCT CATCTGAAGA ACTCCCCTCT CCCACACGAG GGTGCCGGTG 781 GTGCCCAAGC TGTGGAGCAG GCACTGTAAT GGCCACTTTG TTCTTGGTGG CAGATGGGAC 841 AGGGGCCCTG GCAGGCGAGG GTGGCTCCGG GGGCAGCAGC TGGTGCGGGA CCCCTTCAAG 901 GACGTAGTAG GCAGGGTTAT TGAAGCTGTT CTTGGGTGGG GGGGCCGCCA CCCTTTCTGG 961 TTCAGGAGCT CCCTCTGGCT TCAACCTGGG GGTCAAGGGC TCCTCCCGGG GAGCTGCTCG 1021 GGGTGGGCTG GGGGCCTCCC GTTGGTGGCG GTTTCTCTGG GTTCTTCCAA TAACTGAGAG 1081 CGGGCAGAAG GCTCTGTGAA GGCTTGCTGC CGGCTCCCCT GACCTTGGGG GCCT // |
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP,insert 5' Sequence file: RDB15719_A8A9c.seq |
>D05009A1-1_A8A9_CMV-Forward_C01_07_ABI24.ab1 1 CGATTCAACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT 121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA 181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA 241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT 301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA 361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA 421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA 481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT 541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT 601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA 661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT 721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT 781 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT 841 CAGATCTCGA CCCGGGTCAG GATCCTCAGA ATTCACCATG GCCTCGGCCT GCGGGGCGCC 901 GGGCCCGGGG GGGCGCCCTG GGCAGCCAGG CCCCCTCCTG GTACCACCGC GACCTGAGCC 961 GGGCGGCCGC GGAGGAGCTG CTGGCCCGGG CGGGCCGCGA TGGCAGCTTC CCTGGTCCGA 1021 GACAGCGAGA GCGTGGCGGG GGCCCTTTCG CGCTCTGCGT CCTGTATCAG AAGGCATGTG 1081 CACACGTATC GGCATCTGCT GATGGAGAAA GAATTTCTTG GCCTGTTGCC AGGAACCTTT 1141 CG // |
Primer: SV40pro_F_V2 (Pr0154) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB15719_A8A9d.seq |
>D05009A1-1_A8A9_SV40pro_F_V2_D01_10_ABI24.ab1 1 TCCCAGCTTT TTTTGGAGGC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT 61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA 601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG 781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC 841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA 901 CGCCCAACCC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 961 TTCGGAATCG TTTTCCGGGA CGCCCGGCTG GGATGATCCT CCAGCGCGGG GGATCTCATG 1021 CTGGGAGGTT CTTTCGCCCA ACCCCTAAGG GGGGGAGGCT AACTGAAACA CGGAAGGAGG 1081 ACATTACCGG AAGGAACCCG GCGGCTAATG AACGCAATTA AAAGGAAGAA TAAAACGCCA 1141 CCGGGGTGTT TGGGGCGTTC GGTTTGTGTT TTCAT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Fukumoto, M., PI(3,4)P2 plays critical roles in the regulation of focal adhesion dynamics of MDA-MB-231 breast cancer cells. Cancer Sci. 108 (5): 941-951 (2017). PMID 28247964. |
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2023.04.06
GNP_filter3_RDBDEP_html_230405.pl