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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-SHIP2

Expression vector of human SH2-containing inositol phosphatase-2 (SHIP2), fused with N-terminal GFP.

Catalog number RDB15719
Resource name pEGFP-C1-SHIP2
Clone info. Expression vector of human SH2-containing inositol phosphatase-2 (SHIP2), fused with N-terminal GFP. cDNA fragment 148nt to 3921nt of NM_001567.3 is cloned.
Comment Full-length human SHIP2 cDNA were obtained by RT-PCR using total RNA isolated from MDA-MB-231 cells. Expression was confirmed by the depositor with immunofluorescence.
Known differences (at least) : C to G substitution at 3395nt ( Ala to Gly).
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human INPPL1 cDNA
Depositor|Developer Ijuin, Takeshi |
Other clones in our bank

External Database
Human INPPL1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukumoto, M et al. Cancer Sci. 108(5): 941-951, 2017) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Kobe University.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukumoto, M et al. Cancer Sci. 108(5): 941-951, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請及び事業活動を行う場合は、寄託者と別途協議を行う。非営利機関が非営利目的の教育·研究用に用いる場合以外は、神戸大学と別途MTAを締結する。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15719 pEGFP-C1-SHIP2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Fukumoto, M., PI(3,4)P2 plays critical roles in the regulation of focal adhesion dynamics of MDA-MB-231 breast cancer cells. Cancer Sci. 108 (5): 941-951 (2017). PMID 28247964.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15719_A8A9p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C
          Region: EGFP 3',insert 5'(SHIP2)
          Sequence file: RDB15719_A8A9a.seq check
          >D05009A1-1_A8A9_EGFP-C_A01_01_ABI24.ab1
              1 ACGGGAATAT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCTC GACCCGGGTC
             61 AGGATCCTCA GAATTCACCA TGGCCTCGGC CTGCGGGGCG CCGGGCCCGG GGGGCGCCCT
            121 GGGCAGCCAG GCCCCCTCCT GGTACCACCG CGACCTGAGC CGGGCGGCCG CGGAGGAGCT
            181 GCTGGCCCGG GCGGGCCGCG ATGGCAGCTT CCTGGTCCGA GACAGCGAGA GCGTGGCGGG
            241 GGCCTTCGCG CTCTGCGTCC TGTATCAGAA GCATGTGCAC ACGTATCGCA TTCTGCCTGA
            301 TGGAGAAGAT TTCTTGGCTG TGCAGACCTC GCAGGGTGTG CCTGTGCGCC GCTTCCAGAC
            361 CCTGGGTGAG CTCATCGGCC TGTACGCCCA GCCCAACCAG GGCCTTGTGT GCGCCCTGCT
            421 TCTTCCTGTA GAGGGTGAGC GAGAGCCGGA CCCACCGGAT GACCGGGATG CCTCAGATGG
            481 GGAGGATGAG AAGCCCCCGC TGCCCCCGCG CTCTGGCTCC ACCAGCATTT CTGCCCCCAC
            541 TGGGCCCAGC AGTCCCCTGC CAGCTCCTGA GACTCCCACA GCTCCAGCTG CTGAGAGTGC
            601 TCCCAATGGG CTGAGCACCG TCTCGCACGA CTACCTGAAA GGCAGCTATG GGCTGGACCT
            661 GGAAGCTGTG AGGGGTGGAG CCAGCCACCT GCCCCACCTC ACCCGTACCC TCGCTACCTC
            721 ATGCCGGAGG CTGCACAGTG AGGTGGACAA GGTCCTGTCA GGCCTGGAGA TCCTGTCCAA
            781 GGTGTTTGAC CAGCAGAGCT CGCCCATGGT GACCCGCCTT TTGCAGCAGC AGAACCTGCC
            841 ACAGACAGGG GAGCAGGAAC TAGAGAGCCT GGTGCTGAAG CTGTCAGTGC TAAAGGACTT
            901 CCTGTCAGGC ATCCAGAAGA AGGCCCTGAA GGCCCTACAG GACATGAGCT CCACAGCACC
            961 CCCAGCTCCG CAGCCATCCA CACGTAAAGG CCAAGACCAT CCCCGTGCAG GCCTTTGAGG
           1021 TGAAGCTAGA TGTGACCCTG GGTGACCTGA CCAGATTTGG GAAGTCGCAG AAGTTTCACG
           1081 CTGAGCGTGC ATGTGGAGGG GTGGGCCGCT GGTTGCCTGC CTGCCGGAGA ACAGCGGGAC
          1141 CTTCCCCAGG GAGGAACCTG TGGA
          //
          Primer: SV40polyA-R
          Region: insert 3'
          Sequence file: RDB15719_A8A9b.seq check
          >D05009A1-1_A8A9_SV40polyA-R_B01_04_ABI24.ab1
              1 AAAATTTGGA GTTTTAAAGC AAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
             61 CAGTTATCTA GATCCGGTGG ATCAGTCGAC CTTGCTGAGC TGCAGGGTGT CCAGAAGGAG
            121 GCGCTTGTGA GCCGGGTCCT GCACCCCAGC CTCCTCCAAG TCCTCCTCGG TGATGTCACT
            181 GAGAAACTCC AGGTCGTCCC AGCCATTATG CACCAGGCCC TCCTCATAGC GCTCCAAGCC
            241 GATGGCCCGC AGCCAGGCAC TCATGCCTGC CTCGCCCAGC CCGCTGGCCC GCCCGCCCTG
            301 CAGGCACGGA GCCTCCTCTG CCAGGTCTTC CTGGATGCTC TCCCGGATGC GGGGTGGAGG
            361 GAAGCTGAGG GGCCGGCCAT AGTCCGAGGG CAGTCCCCGG GGGGGCTGCA GCTCCAGGGG
            421 GCCTGGGAGG AGCGCTGAGC GTGCAGGCCC AGCAGGGGCA TAGTCCACCT CGCTCAGCGT
            481 CTTGGCCATC TGCAGCACCG AGCAGGACCG GTCATCCCCA CTGGCCACTT CCCCCAGGAA
            541 AGTGCTGGCT GGTGAGGGGC CTGGGGGCAG TGGAGGCCTT GGATGGGCCT TGGGAGGTGG
            601 TGGGCCACGG GCCTCACCCC CACCACGGCC CCGGACCACT GGCCCTGGTA AAGGATCCAG
            661 GCTGGGGGGC AGGAAGATGG CTGAGTCCGG CAGTGGTGGA GGTGGAAAGT CTGGAGGGGG
            721 CAGTGTGCCT CCAGACTCCT CATCTGAAGA ACTCCCCTCT CCCACACGAG GGTGCCGGTG
            781 GTGCCCAAGC TGTGGAGCAG GCACTGTAAT GGCCACTTTG TTCTTGGTGG CAGATGGGAC
            841 AGGGGCCCTG GCAGGCGAGG GTGGCTCCGG GGGCAGCAGC TGGTGCGGGA CCCCTTCAAG
            901 GACGTAGTAG GCAGGGTTAT TGAAGCTGTT CTTGGGTGGG GGGGCCGCCA CCCTTTCTGG
            961 TTCAGGAGCT CCCTCTGGCT TCAACCTGGG GGTCAAGGGC TCCTCCCGGG GAGCTGCTCG
           1021 GGGTGGGCTG GGGGCCTCCC GTTGGTGGCG GTTTCTCTGG GTTCTTCCAA TAACTGAGAG
          1081 CGGGCAGAAG GCTCTGTGAA GGCTTGCTGC CGGCTCCCCT GACCTTGGGG GCCT
          //
          Primer: CMV_Forward
          Region: CMV pro,EGFP,insert 5'
          Sequence file: RDB15719_A8A9c.seq check
          >D05009A1-1_A8A9_CMV-Forward_C01_07_ABI24.ab1
              1 CGATTCAACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
            121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
            181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
            241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
            301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
            361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
            421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
            481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
            541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
            601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
            661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
            721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
            781 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT
            841 CAGATCTCGA CCCGGGTCAG GATCCTCAGA ATTCACCATG GCCTCGGCCT GCGGGGCGCC
            901 GGGCCCGGGG GGGCGCCCTG GGCAGCCAGG CCCCCTCCTG GTACCACCGC GACCTGAGCC
            961 GGGCGGCCGC GGAGGAGCTG CTGGCCCGGG CGGGCCGCGA TGGCAGCTTC CCTGGTCCGA
           1021 GACAGCGAGA GCGTGGCGGG GGCCCTTTCG CGCTCTGCGT CCTGTATCAG AAGGCATGTG
           1081 CACACGTATC GGCATCTGCT GATGGAGAAA GAATTTCTTG GCCTGTTGCC AGGAACCTTT
          1141 CG
          //
          Primer: SV40pro_F_V2
          Region: SV40 pro_ori,NeoR_KanR
          Sequence file: RDB15719_A8A9d.seq check
          >D05009A1-1_A8A9_SV40pro_F_V2_D01_10_ABI24.ab1
              1 TCCCAGCTTT TTTTGGAGGC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT
             61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
            601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
            901 CGCCCAACCC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
            961 TTCGGAATCG TTTTCCGGGA CGCCCGGCTG GGATGATCCT CCAGCGCGGG GGATCTCATG
           1021 CTGGGAGGTT CTTTCGCCCA ACCCCTAAGG GGGGGAGGCT AACTGAAACA CGGAAGGAGG
           1081 ACATTACCGG AAGGAACCCG GCGGCTAATG AACGCAATTA AAAGGAAGAA TAAAACGCCA
          1141 CCGGGGTGTT TGGGGCGTTC GGTTTGTGTT TTCAT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Fukumoto, M., PI(3,4)P2 plays critical roles in the regulation of focal adhesion dynamics of MDA-MB-231 breast cancer cells. Cancer Sci. 108 (5): 941-951 (2017). PMID 28247964.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl