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pEGFP-C1-SHIP2 (#RDB15719)

Expression vector of human SH2-containing inositol phosphatase-2 (SHIP2), fused with N-terminal GFP.

Clone info. Expression vector of human SH2-containing inositol phosphatase-2 (SHIP2), fused with N-terminal GFP. cDNA fragment 148nt to 3921nt of NM_001567.3 is cloned.
Comment Full-length human SHIP2 cDNA were obtained by RT-PCR using total RNA isolated from MDA-MB-231 cells. Expression was confirmed by the depositor with immunofluorescence.
Known differences (at least) : C to G substitution at 3395nt ( Ala to Gly).
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human INPPL1 cDNA
Depositor|Developer Ijuin, Takeshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukumoto, M et al. Cancer Sci. 108(5): 941-951, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Kobe University.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukumoto, M et al. Cancer Sci. 108(5): 941-951, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請及び事業活動を行う場合は、寄託者と別途協議を行う。非営利機関が非営利目的の教育·研究用に用いる場合以外は、神戸大学と別途MTAを締結する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15719 pEGFP-C1-SHIP2 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-SHIP2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15719).

Reference section:

Fukumoto, M., Ijuin, T., Takenawa, T., PI(3,4)P2 plays critical roles in the regulation of focal adhesion dynamics of MDA-MB-231 breast cancer cells. Cancer Sci. 108 (5): 941-951 (2017). PMID 28247964. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15719_A8A9p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C (Pr0025)
Region: EGFP 3',insert 5'(SHIP2)
Sequence file: RDB15719_A8A9a.seq check
>D05009A1-1_A8A9_EGFP-C_A01_01_ABI24.ab1
    1 ACGGGAATAT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCTC GACCCGGGTC
   61 AGGATCCTCA GAATTCACCA TGGCCTCGGC CTGCGGGGCG CCGGGCCCGG GGGGCGCCCT
  121 GGGCAGCCAG GCCCCCTCCT GGTACCACCG CGACCTGAGC CGGGCGGCCG CGGAGGAGCT
  181 GCTGGCCCGG GCGGGCCGCG ATGGCAGCTT CCTGGTCCGA GACAGCGAGA GCGTGGCGGG
  241 GGCCTTCGCG CTCTGCGTCC TGTATCAGAA GCATGTGCAC ACGTATCGCA TTCTGCCTGA
  301 TGGAGAAGAT TTCTTGGCTG TGCAGACCTC GCAGGGTGTG CCTGTGCGCC GCTTCCAGAC
  361 CCTGGGTGAG CTCATCGGCC TGTACGCCCA GCCCAACCAG GGCCTTGTGT GCGCCCTGCT
  421 TCTTCCTGTA GAGGGTGAGC GAGAGCCGGA CCCACCGGAT GACCGGGATG CCTCAGATGG
  481 GGAGGATGAG AAGCCCCCGC TGCCCCCGCG CTCTGGCTCC ACCAGCATTT CTGCCCCCAC
  541 TGGGCCCAGC AGTCCCCTGC CAGCTCCTGA GACTCCCACA GCTCCAGCTG CTGAGAGTGC
  601 TCCCAATGGG CTGAGCACCG TCTCGCACGA CTACCTGAAA GGCAGCTATG GGCTGGACCT
  661 GGAAGCTGTG AGGGGTGGAG CCAGCCACCT GCCCCACCTC ACCCGTACCC TCGCTACCTC
  721 ATGCCGGAGG CTGCACAGTG AGGTGGACAA GGTCCTGTCA GGCCTGGAGA TCCTGTCCAA
  781 GGTGTTTGAC CAGCAGAGCT CGCCCATGGT GACCCGCCTT TTGCAGCAGC AGAACCTGCC
  841 ACAGACAGGG GAGCAGGAAC TAGAGAGCCT GGTGCTGAAG CTGTCAGTGC TAAAGGACTT
  901 CCTGTCAGGC ATCCAGAAGA AGGCCCTGAA GGCCCTACAG GACATGAGCT CCACAGCACC
  961 CCCAGCTCCG CAGCCATCCA CACGTAAAGG CCAAGACCAT CCCCGTGCAG GCCTTTGAGG
 1021 TGAAGCTAGA TGTGACCCTG GGTGACCTGA CCAGATTTGG GAAGTCGCAG AAGTTTCACG
 1081 CTGAGCGTGC ATGTGGAGGG GTGGGCCGCT GGTTGCCTGC CTGCCGGAGA ACAGCGGGAC
1141 CTTCCCCAGG GAGGAACCTG TGGA
//
Primer: SV40polyA-R (Pr0109)
Region: insert 3'
Sequence file: RDB15719_A8A9b.seq check
>D05009A1-1_A8A9_SV40polyA-R_B01_04_ABI24.ab1
    1 AAAATTTGGA GTTTTAAAGC AAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
   61 CAGTTATCTA GATCCGGTGG ATCAGTCGAC CTTGCTGAGC TGCAGGGTGT CCAGAAGGAG
  121 GCGCTTGTGA GCCGGGTCCT GCACCCCAGC CTCCTCCAAG TCCTCCTCGG TGATGTCACT
  181 GAGAAACTCC AGGTCGTCCC AGCCATTATG CACCAGGCCC TCCTCATAGC GCTCCAAGCC
  241 GATGGCCCGC AGCCAGGCAC TCATGCCTGC CTCGCCCAGC CCGCTGGCCC GCCCGCCCTG
  301 CAGGCACGGA GCCTCCTCTG CCAGGTCTTC CTGGATGCTC TCCCGGATGC GGGGTGGAGG
  361 GAAGCTGAGG GGCCGGCCAT AGTCCGAGGG CAGTCCCCGG GGGGGCTGCA GCTCCAGGGG
  421 GCCTGGGAGG AGCGCTGAGC GTGCAGGCCC AGCAGGGGCA TAGTCCACCT CGCTCAGCGT
  481 CTTGGCCATC TGCAGCACCG AGCAGGACCG GTCATCCCCA CTGGCCACTT CCCCCAGGAA
  541 AGTGCTGGCT GGTGAGGGGC CTGGGGGCAG TGGAGGCCTT GGATGGGCCT TGGGAGGTGG
  601 TGGGCCACGG GCCTCACCCC CACCACGGCC CCGGACCACT GGCCCTGGTA AAGGATCCAG
  661 GCTGGGGGGC AGGAAGATGG CTGAGTCCGG CAGTGGTGGA GGTGGAAAGT CTGGAGGGGG
  721 CAGTGTGCCT CCAGACTCCT CATCTGAAGA ACTCCCCTCT CCCACACGAG GGTGCCGGTG
  781 GTGCCCAAGC TGTGGAGCAG GCACTGTAAT GGCCACTTTG TTCTTGGTGG CAGATGGGAC
  841 AGGGGCCCTG GCAGGCGAGG GTGGCTCCGG GGGCAGCAGC TGGTGCGGGA CCCCTTCAAG
  901 GACGTAGTAG GCAGGGTTAT TGAAGCTGTT CTTGGGTGGG GGGGCCGCCA CCCTTTCTGG
  961 TTCAGGAGCT CCCTCTGGCT TCAACCTGGG GGTCAAGGGC TCCTCCCGGG GAGCTGCTCG
 1021 GGGTGGGCTG GGGGCCTCCC GTTGGTGGCG GTTTCTCTGG GTTCTTCCAA TAACTGAGAG
1081 CGGGCAGAAG GCTCTGTGAA GGCTTGCTGC CGGCTCCCCT GACCTTGGGG GCCT
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP,insert 5'
Sequence file: RDB15719_A8A9c.seq check
>D05009A1-1_A8A9_CMV-Forward_C01_07_ABI24.ab1
    1 CGATTCAACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
  121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
  601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
  661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
  721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
  781 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT
  841 CAGATCTCGA CCCGGGTCAG GATCCTCAGA ATTCACCATG GCCTCGGCCT GCGGGGCGCC
  901 GGGCCCGGGG GGGCGCCCTG GGCAGCCAGG CCCCCTCCTG GTACCACCGC GACCTGAGCC
  961 GGGCGGCCGC GGAGGAGCTG CTGGCCCGGG CGGGCCGCGA TGGCAGCTTC CCTGGTCCGA
 1021 GACAGCGAGA GCGTGGCGGG GGCCCTTTCG CGCTCTGCGT CCTGTATCAG AAGGCATGTG
 1081 CACACGTATC GGCATCTGCT GATGGAGAAA GAATTTCTTG GCCTGTTGCC AGGAACCTTT
1141 CG
//
Primer: SV40pro_F_V2 (Pr0154)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB15719_A8A9d.seq check
>D05009A1-1_A8A9_SV40pro_F_V2_D01_10_ABI24.ab1
    1 TCCCAGCTTT TTTTGGAGGC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
  901 CGCCCAACCC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  961 TTCGGAATCG TTTTCCGGGA CGCCCGGCTG GGATGATCCT CCAGCGCGGG GGATCTCATG
 1021 CTGGGAGGTT CTTTCGCCCA ACCCCTAAGG GGGGGAGGCT AACTGAAACA CGGAAGGAGG
 1081 ACATTACCGG AAGGAACCCG GCGGCTAATG AACGCAATTA AAAGGAAGAA TAAAACGCCA
1141 CCGGGGTGTT TGGGGCGTTC GGTTTGTGTT TTCAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukumoto, M., PI(3,4)P2 plays critical roles in the regulation of focal adhesion dynamics of MDA-MB-231 breast cancer cells. Cancer Sci. 108 (5): 941-951 (2017). PMID 28247964. [link to RRC of NBRP]