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p3xFLAGCMV_hIFITM3

Expression vector of human interferon induced transmembrane protein 3 (IFITM3).

Catalog number RDB15717
Resource name p3xFLAGCMV_hIFITM3
Clone info. Expression vector of human interferon induced transmembrane protein 3 (IFITM3), tagged with 3xFLAG at C-termonus.
Human IFITM3 was cloned using cDNA from HeLa cell. Expression was confirmed by the depositor with western blotting.
Vector backbone p3xFLAGCMV14 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human IFITM3 cDNA
Depositor Nishijima, Kenichi |
 
Other clones in our bank human IFITM3 (NCBI Gene 10410) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okuzaki, Y et al. Biosci. Biotechnol. Biochem. 81(5): 914-921, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15717 p3xFLAGCMV_hIFITM3 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Okuzaki, Y., Characterization of chicken interferon-inducible transmembrane protein-10. Biosci. Biotechnol. Biochem. 81 (5): 914-921 (2017). PMID 28084173.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15717_A7Lip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB15717_A7Lia.seq
>D05099A2_A7Li_1_CMV-Forward_D01_10_ABI24.ab1
    1 CTATATTACC CCGCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA ATTAAGCTTG CGGCCGCGAA TTCACCATGA
  121 ATCACACTGT CCAAACCTTC TTCTCTCCTG TCAACAGTGG CCAGCCCCCC AACTATGAGA
  181 TGCTCAAGGA GGAGCACGAG GTGGCTGTGC TGGGGGCGCC CCACAACCCT GCTCCCCCGA
  241 CGTCCACCGT GATCCACATC CGCAGCGAGA CCTCCGTGCC CGACCATGTC GTCTGGTCCC
  301 TGTTCAACAC CCTCTTCATG AACCCCTGCT GCCTGGGCTT CATAGCATTC GCCTACTCCG
  361 TGAAGTCTAG GGACAGGAAG ATGGTTGGCG ACGTGACCGG GGCCCAGGCC TATGCCTCCA
  421 CCGCCAAGTG CCTGAACATC TGGGCCCTGA TTCTGGGCAT CCTCATGACC ATTCTGCTCA
  481 TCGTCATCCC AGTGCTGATC TTCCAGGCCT ATGGAGGATC CCGGGCTGAC TACAAAGACC
  541 ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAT
  601 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT
  661 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG
721 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGG
//
Primer: SV40pro_F RDB15717_A7Lib.seq
>D05099A2_A7Li_1_SV40pro-F_F01_16_ABI24.ab1
    1 CCTTTGGGCG TAGGCTTTCA GAGTAGTGAG GAGGCTTTTT TGGAGGAATT GATCAGCTTG
   61 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
  121 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
  181 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
  241 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG
  301 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
  361 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
  421 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
  481 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG
  541 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC
  601 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT
  661 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
  721 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT
  781 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT
  841 CCCGATTCGC AGCGCATCGC CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
  901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGGATTC
  961 CACCGCCCGC CTTTCTATGA AAGGTTGGGC TTCGGGAATC GTTTTCCCGG GACGCCGGGC
 1021 TGGAATGATC CTTCCAGCGC GGGGATCTCA TGCTGGAAGT TCTTTCGCCC CACCCCCGGC
 1081 TCGAATCCCC CTCGCGAATT GGGATCAGCC TGCCTGCCCT GGAGGCTGGA ACGAACCCTT
1141 CTGCCCGGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Okuzaki, Y., Characterization of chicken interferon-inducible transmembrane protein-10. Biosci. Biotechnol. Biochem. 81 (5): 914-921 (2017). PMID 28084173.

2018.12.11

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