Resource data sheet
Prev.

p3xFLAGCMV_cIFITM10

Expression vector of chicken interferon induced transmembrane protein 10 (IFITM10).

Catalog number RDB15716
Resource name p3xFLAGCMV_cIFITM10
Clone info. Expression vector of chicken interferon induced transmembrane protein 10 (IFITM10) , tagged with 3xFLAG at C-termonus.
Expression was confirmed by the depositor with western blotting.
Vector backbone p3xFLAGCMV14 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Gallus gallus IFITM10 cDNA
Depositor Nishijima, Kenichi |
 
Other clones in our bank Gallus gallus IFITM10 (KEGG orthology K06566) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okuzaki, Y et al. Biosci. Biotechnol. Biochem. 81(5): 914-921, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form by depositor [Word]
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15716 p3xFLAGCMV_cIFITM10 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Okuzaki, Y., Characterization of chicken interferon-inducible transmembrane protein-10. Biosci. Biotechnol. Biochem. 81 (5): 914-921 (2017). PMID 28084173.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15716_A7Lip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB15716_A7Lia.seq
>D05099A1_A7Li_1_CMV-Forward_A01_01_ABI24.ab1
    1 CGAATATAAC CCGCCCGTTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTAAGCTTAC CATGGACGGA CGGACAGGCA
  121 GCGAGAGGGC AGAGCGCGGT GACGCCGCCG CCGCCGCCGC CGCCACCACG GAGAGGACGC
  181 AGGATCCCCC CTTGGGCCCC CCGTGCCCTT TCGACGGGGC GGCCTGGGCC CCCCGGCCCC
  241 CCCCGGGCCC CCAGCAGGGC TGCTTCGCAT GCATCGCCAA GCCCCCGGCC CTCCGGCACG
  301 CCTCGCCCGT CCTCTCGCCC TCCTCCGCCG CGCAGCTGAT GGAGAGCAAG GGCTGCAAAG
  361 GGGACAGCCT GCGGCCGGCG GGCCCGTGCA AGCACTCGGT GGAGAAGAAG ACCATGACCA
  421 ACCCCACCAC CGTCATCGAG ATCTACCCCG ACACCAGCGA GGTGAACGAC TACTATCTCT
  481 GGTCCATCTT CAACTTCGTA TACCTCAACT TCTGCTGCCT CGGCTTCATC GCTTTGGCCT
  541 ATTCGCTGAA AGTCCGGGAT AAGAAACTCC TCAATGACTT AAATGGTGCA GTGGAAGACG
  601 CCAAGACAGC CCGGCTTTTT AACATCACCA GCTCAGCCCT TGCCACCTTC TGCATCATCC
  661 TCATCTTCAT CTTCCTGCGG TACCCCCTCA CCGATTACGA GAATTCATCG ATAGATCTGA
  721 TATCGGTACC AGTCGACTCT AGAGGATCCC GGGCTGACTA CAAAGACCAT GACGGTGATT
  781 ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTGATCC CGGGTGGCAT
  841 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC
  901 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CCTTCTATAA
961 TATTATGGGG GTGGAGGGGG GTGGGTATGG GGGCAGAGGG
//
Primer: SV40pro_F RDB15716_A7Lib.seq
>D05099A1_A7Li_1_SV40pro-F_C01_07_ABI24.ab1
    1 CGCCTGGGCG TGGGGCCTAT TCAAGAGGTA GTGAGGAGGC TTTTTTGGAG GAATTGATCA
   61 GCTTGGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
  121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
  181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
  241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
  301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
  361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
  421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
  481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
  541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
  601 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
  661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
  721 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG
  781 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
  841 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTTCTGAGCG
  901 GGACTCTGGG GTTCGAAAAT GACCGGACCA AGCGACGCCC AACCCTGCCC ATCACGAGAT
  961 TTCGAATTCC ACCGCCGCCT TCTATGAAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG
 1021 CCGGCTGGAA TGATCCTTCC AGCCGCGGGG GATCTCATGG CTGGGAATGT CTTCGGCCTA
 1081 CCCCCGGGCT TCGAATCCCC CTCGCGGAGT TGGTTCAAGC TGCCTTGCCT GAGAGGCTTG
1141 GACGGAAACC TTCCTCG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Okuzaki, Y., Characterization of chicken interferon-inducible transmembrane protein-10. Biosci. Biotechnol. Biochem. 81 (5): 914-921 (2017). PMID 28084173.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl