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p3xFLAGCMV_cIFITM10 (#RDB15716)

Expression vector of chicken interferon induced transmembrane protein 10 (IFITM10).

Clone info. Expression vector of chicken interferon induced transmembrane protein 10 (IFITM10) , tagged with 3xFLAG at C-termonus.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone p3xFLAGCMV14 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Gallus gallus IFITM10 cDNA
Depositor|Developer Nishijima, Kenichi |
 

Distribution information

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Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okuzaki, Y et al. Biosci. Biotechnol. Biochem. 81(5): 914-921, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 １．利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。2. 利用者は、研究成果の公表にあたって開発者の指定する文献を引用する（Okuzaki, Y et al. Biosci. Biotechnol. Biochem. 81(5): 914-921, 2017）。3. 寄託者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。4. 営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15716 p3xFLAGCMV_cIFITM10 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3xFLAGCMV_cIFITM10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15716).

Reference section:

Okuzaki, Y., Kidani, S., Kaneoka, H., Iijima, S., Nishijima, K., Characterization of chicken interferon-inducible transmembrane protein-10. Biosci. Biotechnol. Biochem. 81 (5): 914-921 (2017). PMID 28084173. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15716_A7Lip1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15716_A7Lia.seq check
>D05099A1_A7Li_1_CMV-Forward_A01_01_ABI24.ab1
    1 CGAATATAAC CCGCCCGTTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTAAGCTTAC CATGGACGGA CGGACAGGCA
  121 GCGAGAGGGC AGAGCGCGGT GACGCCGCCG CCGCCGCCGC CGCCACCACG GAGAGGACGC
  181 AGGATCCCCC CTTGGGCCCC CCGTGCCCTT TCGACGGGGC GGCCTGGGCC CCCCGGCCCC
  241 CCCCGGGCCC CCAGCAGGGC TGCTTCGCAT GCATCGCCAA GCCCCCGGCC CTCCGGCACG
  301 CCTCGCCCGT CCTCTCGCCC TCCTCCGCCG CGCAGCTGAT GGAGAGCAAG GGCTGCAAAG
  361 GGGACAGCCT GCGGCCGGCG GGCCCGTGCA AGCACTCGGT GGAGAAGAAG ACCATGACCA
  421 ACCCCACCAC CGTCATCGAG ATCTACCCCG ACACCAGCGA GGTGAACGAC TACTATCTCT
  481 GGTCCATCTT CAACTTCGTA TACCTCAACT TCTGCTGCCT CGGCTTCATC GCTTTGGCCT
  541 ATTCGCTGAA AGTCCGGGAT AAGAAACTCC TCAATGACTT AAATGGTGCA GTGGAAGACG
  601 CCAAGACAGC CCGGCTTTTT AACATCACCA GCTCAGCCCT TGCCACCTTC TGCATCATCC
  661 TCATCTTCAT CTTCCTGCGG TACCCCCTCA CCGATTACGA GAATTCATCG ATAGATCTGA
  721 TATCGGTACC AGTCGACTCT AGAGGATCCC GGGCTGACTA CAAAGACCAT GACGGTGATT
  781 ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTGATCC CGGGTGGCAT
  841 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC
  901 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CCTTCTATAA
961 TATTATGGGG GTGGAGGGGG GTGGGTATGG GGGCAGAGGG
//
Primer: SV40pro_F
Sequence file: RDB15716_A7Lib.seq check
>D05099A1_A7Li_1_SV40pro-F_C01_07_ABI24.ab1
    1 CGCCTGGGCG TGGGGCCTAT TCAAGAGGTA GTGAGGAGGC TTTTTTGGAG GAATTGATCA
   61 GCTTGGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
  121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
  181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
  241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
  301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
  361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
  421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
  481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
  541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
  601 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
  661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
  721 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG
  781 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
  841 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTTCTGAGCG
  901 GGACTCTGGG GTTCGAAAAT GACCGGACCA AGCGACGCCC AACCCTGCCC ATCACGAGAT
  961 TTCGAATTCC ACCGCCGCCT TCTATGAAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG
 1021 CCGGCTGGAA TGATCCTTCC AGCCGCGGGG GATCTCATGG CTGGGAATGT CTTCGGCCTA
 1081 CCCCCGGGCT TCGAATCCCC CTCGCGGAGT TGGTTCAAGC TGCCTTGCCT GAGAGGCTTG
1141 GACGGAAACC TTCCTCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Okuzaki, Y., Characterization of chicken interferon-inducible transmembrane protein-10. Biosci. Biotechnol. Biochem. 81 (5): 914-921 (2017). PMID 28084173. [link to RRC of NBRP]