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pStMuPEP1(Ala70Arg)

Expression vector of Streptococcus mutans peptidase domain 1 (PEP1) of ComA, Ala70Arg mutant.

Catalog number RDB15714
Resource name pStMuPEP1(Ala70Arg)
Clone info. Expression vector of Streptococcus mutans peptidase domain 1 (PEP1) of ComA, Ala70Arg mutant. A clone to investigate the reaction scheme of MuPEP1 and mechanism for non-competitive inhibition, see Fig.4C of Ishii, S. et al., Sci. Rep., 7 (1): 4029, 2017.
PEP of ComA PEP of S. mutans (strain ATCC No. 700610) was generated by PCR from pSMuP1 and then the single mutation was introduced into the expression plasmid of pStMuPEP1 by using primers 5'-ATCAAGCGTGATATGACGCTTTTTGATTATAAT-3' (forward) and 5'-CATATCACGCTTGATAGAGCGTGTTTCAAAGCC-3' (reverse). Reference of genome sequence, GenBank AE014133.
Vector backbone pET21-b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Streptococcus mutans comA genomic
Depositor|Developer Ishii, Seiji |
 
Other clones in our bank Streptococcus mutans (KEGG orthology K20344) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci. Rep. 7(1): 4029, 2017)
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RDB15714 pStMuPEP1(Ala70Arg) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15715_A7Lcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2
Sequence file: RDB15715_A7Lca.seq check
>D05129A3_A7Lc_2_pBR_BamHI_R2_G11_20_ABI24.ab1
    1 CTTGCTGCGT AGGTGGGAGC GTGAGCGGCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT
   61 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC
  121 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGACTTC TTATAAACGT
  181 ACATTTGTTC CTCAAATAGA TGCGAGAGAC TGTGGTGTCG CTGCCTTAGC CTCGATTGCT
  241 AAATTCTATG GTTCAGATTT TTCTCTAGCT CACTTGAGAG AACTTGCAAA GACCAATAAA
  301 GAAGGGACGA CTGCTCTTGG CATTGTAAAA GCCGCTGATG AAATGGGCTT TGAAACAAGG
  361 CCTGTTCAAG CAGATAAAAC TCTCTTTGAC ATGAGTGATG TCCCCTATCC ATTTATCGTT
  421 CACGTTAACA AAGAAGGAAA ACTCCAACAT TACTATGTTG TCTATCAAAC AAAGAAAGAC
  481 TATCTGGTTA TTGGTGATCC TGACCCTTCT GTAAAAATCA CTAAAATGTC AAAAGAACGC
  541 TTTTTCTCTG AATGGACTGG AGTAGCTATT TTTCTAGCTC CCAAACCCAG CTATCAACCC
  601 CATAAAGATA AACACCACCA CCACCACCAC TAGGTCGACA AGCTTGCGGC CGCACTCGAG
  661 CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG
  721 GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
  781 AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATTGG CGAATGGGAC GCGCCCTGTA
  841 GCGGCGCATT AAGCGCGGCG GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
  901 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCCGG
  961 CTTTCCCCGT CAAGCTCTAA ATCGGGGGGC TCCCTTTAGG GTTTCCGATT TAGTGCTTTA
 1021 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGAATGTT CACGTTAGTG GGGCCATCGC
1081 CTTGATAGAC GGTTTTCGGC CCATTGGACG TTGGAAGTTC ACG
//
Primer: lacpro_F
Sequence file: RDB15715_A7Lcb.seq check
>D05129A3_A5Lc_2_laclpro_F_G10_19_ABI24.ab1
    1 GGATGGCAGA TAGCGCCGGA GAGAGTCATT CAGGTGGTGA ATGTGAAACC AGTAACGTTA
   61 TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG
  121 GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT
  181 TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT
  241 GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC
  301 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC
  361 TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT
  421 CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA
  481 TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT
  541 ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG
  601 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT
  661 CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT
  721 CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC
  781 GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG
  841 GATATCTCGG TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA
  901 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGGCAAACC AGCGTGGACC GCTTTGCTGC
  961 AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT TGCCCGTCTC ACTGGTGAAA
 1021 GAAAAAACCA ACCCTGGCGC CCGATACGCA ACGCCTCTCC CGCGCGTTGA CGATTTCATA
1081 ATGCCAGCCT TGCACGGACA GGTTCCGGAC TGGAAGTGCA TGAGCGCAAC GGGCTAT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ishii, S., High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci. Rep. 7 (1): 4029 (2017). PMID 28642545.

2021.03.25

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