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AxDsR-CTF.TDP43

Recombinant adenovirus vector of human TAR DNA-binding protein 43 (TDP-43), C-terminal fragment, tagged with DsRed monomer.

Catalog number RDB15500
Resource name AxDsR-CTF.TDP43
Clone info. Recombinant adenovirus vector of human TAR DNA-binding protein 43 (TDP-43), C-terminal fragment (208aa - 414aa), fused at C-terminal of DsRed monomer, CAG promoter. For the constitution, see Fig.1 of Watabe, K. et al. Neuropathology 34: 83-98, 2014.
Comment TDP-43 cDNA (RefSeq NM_007375) was obtained from HEK 293 cells by RT-PCR. Expression was confirmed by the depositor with immunocytochemistry and Western blot.
Vector backbone pAxCAwtit2 (Adenovirus)
Gene/insert name human TARDBP cDNA Discosoma sp. DsRed
Depositor|Developer Watabe, Kazuhiko |
Other clones in our bank
External Database
human TARDBP Discosoma sp. DsRed
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ishii, T et al. Neuropathology 34(1), 83-98, 2014).
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
          Remarks
          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, T et al. Neuropathology 34(1), 83-98, 2014)。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
          遺伝子組換え生物の受入れ確認書 [Word]
          備考
          組換え体提供にかかる書式が必要です。
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15500 AxDsR-CTF.TDP43 Under QC test. Please contact us. Virus, lysate of infected cell culture

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The AxDsR-CTF.TDP43 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15500).

          Reference section:

          Ishii, T., Kawakami, E., Endo, K., Misawa, H., Watabe, K., Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. PLoS One 12 (6): e0179375 (2017). PMID 28599005.
          Watabe, K., Akiyama, K., Kawakami, E., Ishii, T., Endo, K., Yanagisawa, H., Sango, K., Tsukamoto, M., Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology 34 (1): 83-98 (2014). PMID 23937386.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Ishii, T., Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. PLoS One 12 (6): e0179375 (2017). PMID 28599005.
          original Watabe, K., Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology 34 (1): 83-98 (2014). PMID 23937386.
          reference Soejima-Kusunoki, A., The Protective Effect of Edaravone on TDP-43 Plus Oxidative Stress-Induced Neurotoxicity in Neuronal Cells: Analysis of Its Neuroprotective Mechanisms Using RNA Sequencing. Pharmaceuticals (Basel) 15 (7): 842 (2022). PMID 35890141.
          reference Watabe, K., Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. Neuropathology 40 (6): 570-586 (2020). PMID 32686212.

          2023.05.22

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