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AxDsR-CTF.TDP43

Recombinant adenovirus vector of human TAR DNA-binding protein 43 (TDP-43), C-terminal fragment, tagged with DsRed monomer.

Catalog number RDB15500
Resource name AxDsR-CTF.TDP43
Clone info. Recombinant adenovirus vector of human TAR DNA-binding protein 43 (TDP-43), C-terminal fragment (208aa - 414aa), fused at C-terminal of DsRed monomer, CAG promoter. For the constitution, see Fig.1 of Watabe, K. et al. Neuropathology 34: 83-98, 2014.
Comment TDP-43 cDNA (RefSeq NM_007375) was obtained from HEK 293 cells by RT-PCR. Expression was confirmed by the depositor with immunocytochemistry and Western blot.
Vector backbone pAxCAwtit2 (Adenovirus)
Gene/insert name human TARDBP cDNA Discosoma sp. DsRed
Depositor|Developer Watabe, Kazuhiko |
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External Database
human TARDBP Discosoma sp. DsRed
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ishii, T et al. Neuropathology 34(1), 83-98, 2014).
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please contact us to request the Exclusive MTA.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, T et al. Neuropathology 34(1), 83-98, 2014)。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
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          提供依頼書 [Word]
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15500 AxDsR-CTF.TDP43 Under QC test. Please contact us. Virus, lysate of infected cell culture

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The AxDsR-CTF.TDP43 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15500).

          Reference section:

          Ishii, T., Kawakami, E., Endo, K., Misawa, H., Watabe, K., Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. PLoS One 12 (6): e0179375 (2017). PMID 28599005. [link to RRC of NBRP]
          Watabe, K., Akiyama, K., Kawakami, E., Ishii, T., Endo, K., Yanagisawa, H., Sango, K., Tsukamoto, M., Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology 34 (1): 83-98 (2014). PMID 23937386. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Ishii, T., Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. PLoS One 12 (6): e0179375 (2017). PMID 28599005. [link to RRC of NBRP]
          original Watabe, K., Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology 34 (1): 83-98 (2014). PMID 23937386. [link to RRC of NBRP]
          reference Soejima-Kusunoki, A., The Protective Effect of Edaravone on TDP-43 Plus Oxidative Stress-Induced Neurotoxicity in Neuronal Cells: Analysis of Its Neuroprotective Mechanisms Using RNA Sequencing. Pharmaceuticals (Basel) 15 (7): 842 (2022). PMID 35890141.
          reference Watabe, K., Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. Neuropathology 40 (6): 570-586 (2020). PMID 32686212.

          2023.06.15

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