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AxDsR-WT.TDP43

Recombinant adenovirus vector of human TAR DNA-binding protein 43 (TDP-43), wild type, tagged with DsRed monomer.

Catalog number RDB15499
Resource name AxDsR-WT.TDP43
Clone info. Recombinant adenovirus vector of human TAR DNA-binding protein 43 (TDP-43), wild type, fused at C-terminal of DsRed, CAG promoter. For the constitution, see Fig.1 of Watabe, K. et al. Neuropathology 34: 83-98, 2014.
TDP-43 cDNA (RefSeq NM_007375) was obtained from HEK 293 cells by RT-PCR. Expression was confirmed by the depositor with immunocytochemistry and Western blot.
Vector backbone pAxCAwtit2 (Adenovirus)
Gene/insert name human TARDBP cDNA Discosoma sp. DsRed
Depositor|Developer Watabe, Kazuhiko |
 
Other clones in our bank human TARDBP (NCBI Gene 23435) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ishii, T et al. Neuropathology 34(1), 83-98, 2014).
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15499 AxDsR-WT.TDP43 Under QC test. Please contact us. Virus, lysate of infected cell culture Please contact us.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ishii, T., Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. PLoS One 12 (6): e0179375 (2017). PMID 28599005.
original Watabe, K., Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology 34 (1): 83-98 (2014). PMID 23937386.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Ishii, T., Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. PLoS One 12 (6): e0179375 (2017). PMID 28599005.
original Watabe, K., Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology 34 (1): 83-98 (2014). PMID 23937386.
reference Watabe, K., Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. Neuropathology . 2020 Jul 19. doi: 10.1111/neup.12694. Online ahead of print. PMID 32686212.

2021.03.25

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