Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

AxDsR-WT.TDP43 (#RDB15499)

Recombinant adenovirus vector of human TAR DNA-binding protein 43 (TDP-43), wild type, tagged with DsRed monomer.

Clone info. Recombinant adenovirus vector of human TAR DNA-binding protein 43 (TDP-43), wild type, fused at C-terminal of DsRed monomer, CAG promoter. For the constitution, see Fig.1 of Watabe, K. et al. Neuropathology 34: 83-98, 2014.
Comment TDP-43 cDNA (RefSeq NM_007375) was obtained from HEK 293 cells by RT-PCR. Expression was confirmed by the depositor with immunocytochemistry and Western blot.
Vector backbone pAxCAwtit2 (Adenovirus)
Gene/insert name human TARDBP cDNA Discosoma sp. DsRed
Depositor|Developer Watabe, Kazuhiko |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
In order to receive this biological resource, please request the Exclusive MTA to us.
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ishii, T et al. Neuropathology 34(1), 83-98, 2014).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
遺伝子組換え生物の受入れ確認書 [Word]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, T et al. Neuropathology 34(1), 83-98, 2014)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考
組換え体提供にかかる書式が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15499 AxDsR-WT.TDP43 Under QC test. Please contact us. Virus, lysate of infected cell culture

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The AxDsR-WT.TDP43 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15499).

Reference section:

Ishii, T., Kawakami, E., Endo, K., Misawa, H., Watabe, K., Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. PLoS One 12 (6): e0179375 (2017). PMID 28599005. [link to RRC of NBRP]
Watabe, K., Akiyama, K., Kawakami, E., Ishii, T., Endo, K., Yanagisawa, H., Sango, K., Tsukamoto, M., Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology 34 (1): 83-98 (2014). PMID 23937386. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ishii, T., Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. PLoS One 12 (6): e0179375 (2017). PMID 28599005. [link to RRC of NBRP]
original Watabe, K., Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology 34 (1): 83-98 (2014). PMID 23937386. [link to RRC of NBRP]
reference Soejima-Kusunoki, A., The Protective Effect of Edaravone on TDP-43 Plus Oxidative Stress-Induced Neurotoxicity in Neuronal Cells: Analysis of Its Neuroprotective Mechanisms Using RNA Sequencing. Pharmaceuticals (Basel) 15 (7): 842 (2022). PMID 35890141.
reference Watabe, K., Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. Neuropathology 40 (6): 570-586 (2020). PMID 32686212.