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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTER-EGFR C797S/T790M/L858R

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.

Catalog number RDB15496
Resource name pENTER-EGFR C797S/T790M/L858R
Clone info. Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, C797S, T790M and L858R, EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.
Comment cDNA was obtained from A549 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction).
Vector backbone pENTR (plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Gene/insert name human EGFR cDNA
Depositor|Developer Katayama, Ryohei |
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External Database
human EGFR

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
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          Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Uchibori, K et al. Nat. Commun. 8: 14768, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

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          RDB15496 pENTER-EGFR C797S/T790M/L858R DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15496_A7K8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: rrnBT1ter_F2
          Region: rrnB T1 ter, attL1, insert 5'
          Sequence file: RDB15496_A7K8a.seq check
          >D04925A9_A7K8_1_rrnBT1ter_F2_C08_08_ABI24.ab1
              1 AGCCAGGTCT TCGACTGAGC CTTTCGTTTT ATTTGATGCC TGGCAGTTCC CTACTCTCGC
             61 GTTAACGCTA GCATGGATGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTCTTAA
            121 GCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG ACCTGTTCGT TGCAACAAAT
            181 TGATGAGCAA TGCTTTTTTA TAATGCCAAC TTTGTACAAA AAAGCAGGCT CCGCGGCCGC
            241 CCCCTTCACC ATGCGACCCT CCGGGACGGC CGGGGCAGCG CTCCTGGCGC TGCTGGCTGC
            301 GCTCTGCCCG GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA
            361 GCTCACGCAG TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA
            421 CTGTGAGGTG GTCCTTGGGA ATTTGGAAAT TACCTATGTG CAGAGGAATT ATGATCTTTC
            481 CTTCTTAAAG ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATTGCCCTCA ACACAGTGGA
            541 GCGAATTCCT TTGGAAAACC TGCAGATCAT CAGAGGAAAT ATGTACTACG AAAATTCCTA
            601 TGCCTTAGCA GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT
            661 GAGAAATTTA CAGGAAATCC TGCATGGCGC CGTGCGGTTC AGCAACAACC CTGCCCTGTG
            721 CAATGTGGAG AGCATCCAGT GGCGGGACAT AGTCAGCAGT GACTTTCTCA GCAACATGTC
            781 GATGGACTTC CAGAACCACC TGGGCAGCTG CCAAAAGTGT GATCCAAGCT GTCCCAATGG
            841 GAGCTGCTGG GGTGCAGGAG AGGAGAACTG CCAGAAACTG ACCAAAATCA TCTGTGCCCA
            901 GCAGTGCTCC GGGCGCTGCC GTGGCAAGTC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC
            961 TGCAGCTGCA CAGGCCCCCG GGAGAGCGAC TGCCTGGTTC TGCCGCAATT CCGAGACGAA
           1021 GCCACGTGCA AGGACACCTG CCCCCCCCAC TCATGCTCTA CAACCCCCAA CACCGTACAA
           1081 GATGGATGTG AAACCCCGAA GGGCCAAATA CCAGCCTTTT GGTTGCCACC CTGGCGGTGA
          1141 AGAAGGTGTG TTCCCCCGG
          //
          Primer: pDONR_R
          Region: attL2, insert 3'
          Sequence file: RDB15496_A7K8b.seq check
          >D04925A9_A7K8_1_pDONR_R_B06_06_ABI24.ab1
              1 GGAGACGCAG GCACGCTATG ACCATGTAAT ACGACTCACT ATAGGGGATA TCAGCTGGAT
             61 GGCAAATAAT GATTTTATTT TGACTGATAG TGACCTGTTC GTTGCAACAA ATTGATAAGC
            121 AATGCTTTCT TATAATGCCA ACTTTGTACA AGAAAGCTGG GTCGGCGCGC CCACCCTTTC
            181 ATGCTCCAAT AAATTCACTG CTTTGTGGCG CGACCCTTAG GTATTCTGCA TTTTCAGCTG
            241 TGGAGCCCTT AAAGATGCCA TTTGGCTTGG CTTCCTTGGG AAAGAAGTCC TGCTGGTAGT
            301 CAGGGTTGTC CAGGCTAATT TGGTGGCTGC CTTTCTGGGC CCAGTGGGCA GGGCTGTCGA
            361 ATGTGCTGTT GACACAGGTG GGCTGGACAG TGTTGAGATA CTCGGGGTTG CCCACTGCAG
            421 TGCTGTGGGG GTCCTGGTAG TGTGGGTCTC TGCTGGGCGC GGGGTTCAGA GGCTGATTGT
            481 GATAGACAGG ATTCTGCACA GAGCCAGCGG GCCTTTTGGG AACGGACTGG TTTATGTATT
            541 CAGGCACTGG GAGGAAGGTG TCGTCTATGC TGTCCTCAGT CAAGGCGCCT GTGGGGTCTG
            601 AGCTGTATCG CTGCAAGAAG CTGTCTTCCT TGATGGGACA GCTTTGCAGC CCATTTCTAT
            661 CAATGCAAGC CACGGTGGAA TTGTTGCTGG TTGCACTCAG AGAGCTCAGG AGGGGAGTCC
            721 GTGACGTGGA GGGGCTGCTG AAGAAGCCCT GCTGTGGGAT GAGGTACTCG TCGGCATCCA
            781 CCACGTCGTC CATGTCTTCT TCATCCATCA GGGCACGGTA GAAGTTGGAG TCTGTAGGAC
            841 TTGGCAAATG CATTCTTTCA TCCCCCCTGA ATGACAAGGT AGCGCTGGGG GGTCTCGGGG
            901 CCATTTTGGA GAATTCGATG ATCAACTCAC GGAAACTTTG GGCGACTATC TGCGTCTATC
            961 ATCCAGCACT TGACCATGAT CATGTAGACA TCGATGGTAC ATATGGGTGG CTGAGGGAGG
           1021 CCGTTTCTCC TTTCTCCAGG ATGGAGGAGA TCTCGCTGGC AGGATTCCGG TCATATGACT
          1081 GAATTCCAAA GGATCATCCA ACCTCCCCAA AACG
          //
          Primer: EGFR-F5
          Region: insert middle region (T790M, C797S, L858R)
          Sequence file: RDB15496_A7K8c.seq check
          >D04925A9_A7K8_1_EGFR-F5_C06_09_ABI24.ab1
              1 GACAAATCGA TCATCGCCAC TGGGATGGTG GGGGCCCTCC TCTTGCTGCT GGTGGTGGCC
             61 CTGGGGATCG GCCTCTTCAT GCGAAGGCGC CACATCGTTC GGAAGCGCAC GCTGCGGAGG
            121 CTGCTGCAGG AGAGGGAGCT TGTGGAGCCT CTTACACCCA GTGGAGAAGC TCCCAACCAA
            181 GCTCTCTTGA GGATCTTGAA GGAAACTGAA TTCAAAAAGA TCAAAGTGCT GGGCTCCGGT
            241 GCGTTCGGCA CGGTGTATAA GGGACTCTGG ATCCCAGAAG GTGAGAAAGT TAAAATTCCC
            301 GTCGCTATCA AGGAATTAAG AGAAGCAACA TCTCCGAAAG CCAACAAGGA AATCCTCGAT
            361 GAAGCCTACG TGATGGCCAG CGTGGACAAC CCCCACGTGT GCCGCCTGCT GGGCATCTGC
            421 CTCACCTCCA CCGTGCAGCT CATCATGCAG CTCATGCCCT TCGGCTCCCT CCTGGACTAT
            481 GTCCGGGAAC ACAAAGACAA TATTGGCTCC CAGTACCTGC TCAACTGGTG TGTGCAGATC
            541 GCAAAGGGCA TGAACTACTT GGAGGACCGT CGCTTGGTGC ACCGCGACCT GGCAGCCAGG
            601 AACGTACTGG TGAAAACACC GCAGCATGTC AAGATCACAG ATTTTGGGCG GGCCAAACTG
            661 CTGGGTGCGG AAGAGAAAGA ATACCATGCA GAAGGAGGCA AAGTGCCTAT CAAGTGGATG
            721 GCATTGGAAT CAATTTTACA CAGAATCTAT ACCCACCAGA GTGATGTCTG GAGCTACGGG
            781 GTGACCGTTT GGGAGTTGAT GACCTTTGGA TCCAAGCCAT ATGACGGAAT CCCTGCCAGC
            841 GAGATCTCCT CCATCCTGGA GAAAGGAGAA CGCCTCCCTC AGCCACCCAT ATGTACCATC
            901 GATGTCTACA TGATCATGGT CAAGTGCTGG ATGATAGACG CAGATAGTCG CCCAAAGTTC
            961 CGTGAGTTGA TCATCGAAAT TCTCCAAAAT GACCCGAGAC CCCCCAGCGC TACCCTTTGT
           1021 TCATTTCATG GGGGATGAAA GAATGCATTT GGTAGTCCCT ACAGACTCCA ACTCTAACGT
          1081 GCCTGATGGA ATGGAGAAGT ACTATGGTAC GGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl