Sequencing and PCR primers for primer information.
Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.
| Catalog number | RDB15495 |
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| Resource name | pENTER-EGFR C797S/L858R |
| Clone info. | Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, C797S and L858R, EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations. cDNA was obtained from A549 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction). |
| Vector backbone | pENTR (plasmid) |
| Size of vector backbone | 2.6 kb |
| Selectable markers | Kan^r |
| Gene/insert name | human EGFR cDNA |
| Depositor | Katayama, Ryohei | |
| Other clones in our bank | human EGFR (NCBI Gene 1956) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017) |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15495 | pENTER-EGFR C797S/L858R | DNA solution |
Electronic file
Original reference
| original | Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083. |
|---|
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15495_A7K8p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Region: rrnB T1 ter, attL1, insert 5' | Primer: rrnBT1ter_F2 | Sequence: RDB15495_A7K8a.seq |
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>D04925A8_A7K8_2_rrnBT1ter_F2_H04_22_ABI24.ab1 1 AGCCAGATCT TCCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG 61 CGTTAACGCT AGCATGGATG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTCTTA 121 AGCTCGGGCC CCAAATAATG ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA 181 TTGATGAGCA ATGCTTTTTT ATAATGCCAA CTTTGTACAA AAAAGCAGGC TCCGCGGCCG 241 CCCCCTTCAC CATGCGACCC TCCGGGACGG CCGGGGCAGC GCTCCTGGCG CTGCTGGCTG 301 CGCTCTGCCC GGCGAGTCGG GCTCTGGAGG AAAAGAAAGT TTGCCAAGGC ACGAGTAACA 361 AGCTCACGCA GTTGGGCACT TTTGAAGATC ATTTTCTCAG CCTCCAGAGG ATGTTCAATA 421 ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT TATGATCTTT 481 CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC AACACAGTGG 541 AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC GAAAATTCCT 601 ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG GAGCTGCCCA 661 TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC CCTGCCCTGT 721 GCAATGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC AGCAACATGT 781 CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC TGTCCCAATG 841 GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC ATCTGTGCCC 901 AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCCAGTG ACTGCTGCCA CAACCAGTGT 961 GCTGCAGCTG CACAGGCCCC CGGGAGAGCG ACTGCCTGGG TCTGCCGCAA TTTCCGAGAC 1021 GAAGCCACGT GCAAGACACC TGCCCCCCCC ACTCATGCTT CTACAACCCC ACCACGTACA 1081 GATGGATGTG ATCCCCGAAG GTCAAATTAC AGCGTTTGGG GTGCTAC // |
| Region: attL2, insert 3' | Primer: pDONR_R | Sequence: RDB15495_A7K8b.seq |
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>D04925A8_A7K8_2_pDONR_R_E06_15_ABI24.ab1 1 GCGGACGTCA GGGTACGCTA TGACCATGTA ATACGACTCA CTATAGGGGA TATCAGCTGG 61 ATGGCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATAA 121 GCAATGCTTT CTTATAATGC CAACTTTGTA CAAGAAAGCT GGGTCGGCGC GCCCACCCTT 181 TCATGCTCCA ATAAATTCAC TGCTTTGTGG CGCGACCCTT AGGTATTCTG CATTTTCAGC 241 TGTGGAGCCC TTAAAGATGC CATTTGGCTT GGCTTCCTTG GGAAAGAAGT CCTGCTGGTA 301 GTCAGGGTTG TCCAGGCTAA TTTGGTGGCT GCCTTTCTGG GCCCAGTGGG CAGGGCTGTC 361 GAATGTGCTG TTGACACAGG TGGGCTGGAC AGTGTTGAGA TACTCGGGGT TGCCCACTGC 421 AGTGCTGTGG GGGTCCTGGT AGTGTGGGTC TCTGCTGGGC GCGGGGTTCA GAGGCTGATT 481 GTGATAGACA GGATTCTGCA CAGAGCCAGC GGGCCTTTTG GGAACGGACT GGTTTATGTA 541 TTCAGGCACT GGGAGGAAGG TGTCGTCTAT GCTGTCCTCA GTCAAGGCGC CTGTGGGGTC 601 TGAGCTGTAT CGCTGCAAGA AGCTGTCTTC CTTGATGGGA CAGCTTTGCA GCCCATTTCT 661 ATCAATGCAA GCCACGGTGG AATTGTTGCT GGTTGCACTC AGAGAGCTCA GGAGGGGAGT 721 CCGTGACGTG GAGGGGCTGC TGAAGAAGCC CTGCTGTGGG ATGAGGTACT CGTCGGCATC 781 CACCACGTCG TCCATGTCTT CTTCATCCAT CAGGGCACGG TAGAAGTTGG AGTCTGTAGG 841 ACTTGGGCAA ATGCATTCTT TCATCCCCCC TGAATGACAA GGTAGCGCTG GGGGGTCTCG 901 GGCCATTTTG GAGAATTCGA TGATCAACTC ACGGAAACTT TGGGGCGACT ATCTGCGTCT 961 ATCATCCAGC ACTTTGACCA TGATCATGTA GACATCGATG GGTACATATG GGTGGCTGAG 1021 GGAGGGCGTT CTTCGTTTCT CAGGATGGAG GAGATCTCGC TGGGCAGGAA TTTCGTCATA 1081 TGACATGCAA TTCGAAGGTT CAATCCAACC TCCCAAAACA GT // |
| Region: insert middle region (C797S, L858R) | Primer: EGFR-F5 | Sequence: RDB15495_A7K8c.seq |
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>D04925A8_A7K8_2_EGFR-F5_F06_18_ABI24.ab1 1 AACATAATCG TCATCGCCAC TGGGATGGTG GGGGCCCTCC TCTTGCTGCT GGTGGTGGCC 61 CTGGGGATCG GCCTCTTCAT GCGAAGGCGC CACATCGTTC GGAAGCGCAC GCTGCGGAGG 121 CTGCTGCAGG AGAGGGAGCT TGTGGAGCCT CTTACACCCA GTGGAGAAGC TCCCAACCAA 181 GCTCTCTTGA GGATCTTGAA GGAAACTGAA TTCAAAAAGA TCAAAGTGCT GGGCTCCGGT 241 GCGTTCGGCA CGGTGTATAA GGGACTCTGG ATCCCAGAAG GTGAGAAAGT TAAAATTCCC 301 GTCGCTATCA AGGAATTAAG AGAAGCAACA TCTCCGAAAG CCAACAAGGA AATCCTCGAT 361 GAAGCCTACG TGATGGCCAG CGTGGACAAC CCCCACGTGT GCCGCCTGCT GGGCATCTGC 421 CTCACCTCCA CCGTGCAACT CATCACGCAG CTCATGCCCT TCGGCTCCCT CCTGGACTAT 481 GTCCGGGAAC ACAAAGACAA TATTGGCTCC CAGTACCTGC TCAACTGGTG TGTGCAGATC 541 GCAAAGGGCA TGAACTACTT GGAGGACCGT CGCTTGGTGC ACCGCGACCT GGCAGCCAGG 601 AACGTACTGG TGAAAACACC GCAGCATGTC AAGATCACAG ATTTTGGGCG GGCCAAACTG 661 CTGGGTGCGG AAGAGAAAGA ATACCATGCA GAAGGAGGCA AAGTGCCTAT CAAGTGGATG 721 GCATTGGAAT CAATTTTACA CAGAATCTAT ACCCACCAGA GTGATGTCTG GAGCTACGGG 781 GTGACCGTTT GGGAGTTGAT GACCTTTGGA TCCAAGCCAT ATGACGGAAT CCCTGCCAGC 841 GAGATCTCCT CCATCCTGGG AGAAAGGAGA ACGCCTCCCT CAGCCACCCA TATGTACCAT 901 CGATGTCTAC ATGATCATGG TCAAGTGCTG GATGATAGAC GCAGATAGTC GCCCAAAGTT 961 TCCGTGAGTT TGATCATCGA AATTCTCCCA AAATGGCCCG AGACCCCCAG CGCTACCCTT 1021 TGTTCATTCA AGGGGGAATG AAGAATGCCA TTTGGCTAGG TTCGTACAGA CCTCCGAACT 1081 CTACGCTGCC GTGAATTCAA TGCAGGAAGG ACATTG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083. |
|---|
2019.09.20
GNP_filter3_RDBDEP_html_190816.pl