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pENTER-EGFR C797S/T790M/del19

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.

Catalog number RDB15492
Resource name pENTER-EGFR C797S/T790M/del19
Clone info. Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, C797S, T790M, and deletion in exon 19, EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.
cDNA was obtained from HCC-827 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction).
Vector backbone pENTR (plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Gene/insert name human EGFR cDNA
Depositor Katayama, Ryohei |
 
Other clones in our bank human EGFR (NCBI Gene 1956) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
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Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB15492 pENTER-EGFR C797S/T790M/del19 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15492_A7K8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: rrnB T1 ter, attL1, insert 5' | Primer: rrnBT1ter_F2 | Sequence: RDB15492_A7K8a.seq
>D04925A5_A7K8_2_rrnBT1ter_F2_E04_13_ABI24.ab1
    1 AGCAGATCTT CGACTGAGCC TTTCGTTTTA TTTGATGCCT GGCAGTTCCC TACTCTCGCG
   61 TTAACGCTAG CATGGATGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTCTTAAG
  121 CTCGGGCCCC AAATAATGAT TTTATTTTGA CTGATAGTGA CCTGTTCGTT GCAACAAATT
  181 GATGAGCAAT GCTTTTTTAT AATGCCAACT TTGTACAAAA AAGCAGGCTC CGCGGCCGCC
  241 CCCTTCACCA TGCGACCCTC CGGGACGGCC GGGGCAGCGC TCCTGGCGCT GCTGGCTGCG
  301 CTCTGCCCGG CGAGTCGGGC TCTGGAGGAA AAGAAAGTTT GCCAAGGCAC GAGTAACAAG
  361 CTCACGCAGT TGGGCACTTT TGAAGATCAT TTTCTCAGCC TCCAGAGGAT GTTCAATAAC
  421 TGTGAGGTGG TCCTTGGGAA TTTGGAAATT ACCTATGTGC AGAGGAATTA TGATCTTTCC
  481 TTCTTAAAGA CCATCCAGGA GGTGGCTGGT TATGTCCTCA TTGCCCTCAA CACAGTGGAG
  541 CGAATTCCTT TGGAAAACCT GCAGATCATC AGAGGAAATA TGTACTACGA AAATTCCTAT
  601 GCCTTAGCAG TCTTATCTAA CTATGATGCA AATAAAACCG GACTGAAGGA GCTGCCCATG
  661 AGAAATTTAC AGGAAATCCT GCATGGCGCC GTGCGGTTCA GCAACAACCC TGCCCTGTGC
  721 AATGTGGAGA GCATCCAGTG GCGGGACATA GTCAGCAGTG ACTTTCTCAG CAACATGTCG
  781 ATGGACTTCC AGAACCACCT GGGCAGCTGC CAAAAGTGTG ATCCAAGCTG TCCCAATGGG
  841 AGCTGCTGGG GTGCAGGAGA GGAGAACTGC CAGAAACTGA CCAAAATCAT CTGTGCCCAG
  901 CAGTGCTCCG GGCGCTGCCG TGGCAAGTCC CCCCAGTGAC TGCTGCCACA ACCAGTGTGC
  961 TGCAGCTGCA CAGGGCCCCC GGGAGAGCGA CTGCCCTGGT CTGCCGCAAA TTCCCGAGAC
 1021 GAAGCCACGT GCAAAGGACA CCTGCCCCCC CCACTTCATG CTTCTTACAA CCCCCACCAC
1081 GTTAACCAGA TGATGTGAAC CCCGAGGTCA AAATAACAGG CCTTTTGGGA AGC
//
Region: attL2, insert 3' | Primer: pDONR_R | Sequence: RDB15492_A7K8b.seq
>D04925A5_A7K8_2_pDONR_R_E08_14_ABI24.ab1
    1 GGCGGACGGC AGGACGCTAT GACCATGTAA TACGACTCAC TATAGGGGAT ATCAGCTGGA
   61 TGGCAAATAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATAAG
  121 CAATGCTTTC TTATAATGCC AACTTTGTAC AAGAAAGCTG GGTCGGCGCG CCCACCCTTT
  181 CATGCTCCAA TAAATTCACT GCTTTGTGGC GCGACCCTTA GGTATTCTGC ATTTTCAGCT
  241 GTGGAGCCCT TAAAGATGCC ATTTGGCTTG GCTTCCTTGG GAAAGAAGTC CTGCTGGTAG
  301 TCAGGGTTGT CCAGGCTAAT TTGGTGGCTG CCTTTCTGGG CCCAGTGGGC AGGGCTGTCG
  361 AATGTGCTGT TGACACAGGT GGGCTGGACA GTGTTGAGAT ACTCGGGGTT GCCCACTGCA
  421 GTGCTGTGGG GGTCCTGGTA GTGTGGGTCT CTGCTGGGCG CGGGGTTCAG AGGCTGATTG
  481 TGATAGACAG GATTCTGCAC AGAGCCAGCG GGCCTTTTGG GAACGGACTG GTTTATGTAT
  541 TCAGGCACTG GGAGGAAGGT GTCGTCTATG CTGTCCTCAG TCAAGGCGCC TGTGGGGTCT
  601 GAGCTGTATC GCTGCAAGAA GCTGTCTTCC TTGATGGGAC AGCTTTGCAG CCCATTTCTA
  661 TCAATGCAAG CCACGGTGGA ATTGTTGCTG GTTGCACTCA GAGAGCTCAG GAGGGGAGTC
  721 CGTGACGTGG AGGGGCTGCT GAAGAAGCCC TGCTGTGGGA TGAGGTACTC GTCGGCATCC
  781 ACCACGTCGT CCATGTCTTC TTCATCCATC AGGGCACGGT AGAAGTTGGA GTCTGTAGGA
  841 CTTGGCAAAT GCATTCTTTC ATCCCCCTGA ATGACAAGGT AGCGCTGGGG GGTCTCGGGC
  901 CATTTTGGAG AATTCGATGA TCAACTCACG GAACTTTGGG CGACTATCTG CGTCTATCAT
  961 CCAGCACTTG ACCATGATCA TGTAGACATC GATGGTACAT ATGGGTGGCT GAGGAGGGCG
 1021 TTTCTCCTTT CTCCAGGATG GAGGAGATTC TCGGCTGGGC AGGATTCCCG TCATATGACT
1081 GGAATTCTAA AGGTCAATCC AACTTCCCAA AACCG
//
Region: insert middle region (C797S, T790M, del19) | Primer: EGFR-F5 | Sequence: RDB15492_A7K8c.seq
>D04925A5_A7K8_2_EGFR-F5_E09_15_ABI24.ab1
    1 AACATTAATC CGTCATCGCC ACTGGGATGG TGGGGGCCCT CCTCTTGCTG CTGGTGGTGG
   61 CCCTGGGGAT CGGCCTCTTC ATGCGAAGGC GCCACATCGT TCGGAAGCGC ACGCTGCGGA
  121 GGCTGCTGCA GGAGAGGGAG CTTGTGGAGC CTCTTACACC CAGTGGAGAA GCTCCCAACC
  181 AAGCTCTCTT GAGGATCTTG AAGGAAACTG AATTCAAAAA GATCAAAGTG CTGGGCTCCG
  241 GTGCGTTCGG CACGGTGTAT AAGGGACTCT GGATCCCAGA AGGTGAGAAA GTTAAAATTC
  301 CCGTCGCTAT CAAGACATCT CCGAAAGCCA ACAAGGAAAT CCTCGATGAA GCCTACGTGA
  361 TGGCCAGCGT GGACAACCCC CACGTGTGCC GCCTGCTGGG CATCTGCCTC ACCTCCACCG
  421 TGCAGCTCAT CATGCAGCTC ATGCCCTTCG GCTCCCTCCT GGACTATGTC CGGGAACACA
  481 AAGACAATAT TGGCTCCCAG TACCTGCTCA ACTGGTGTGT GCAGATCGCA AAGGGCATGA
  541 ACTACTTGGA GGACCGTCGC TTGGTGCACC GCGACCTGGC AGCCAGGAAC GTACTGGTGA
  601 AAACACCGCA GCATGTCAAG ATCACAGATT TTGGGCTGGC CAAACTGCTG GGTGCGGAAG
  661 AGAAAGAATA CCATGCAGAA GGAGGCAAAG TGCCTATCAA GTGGATGGCA TTGGAATCAA
  721 TTTTACACAG AATCTATACC CACCAGAGTG ATGTCTGGAG CTACGGGGTG ACCGTTTGGG
  781 AGTTGATGAC CTTTGGATCC AAGCCATATG ACGGAATCCC TGCCAGCGAG ATCTCCTCCA
  841 TCCTGGAGAA AGGAGAACGC CTCCCTCAGC CACCCATATG TACCATCGAT GTCTACATGA
  901 TCATGGTCAA GTGCTGGATG ATAGACGCAG ATAGTCGCCC AAAGTTTCCG TGAGTTGATC
  961 ATCGAATTCT CCCAAAATGG CCCGAGACCC CCAGCGCTAC CCTTTGTCAT TCAGGGGGAT
 1021 GAAAGATGCA TTTGCCAGTC CCTACAGGAC TCTACTTCTA CGTGCCCTGA TGTATGAAGA
1081 AGACATCACG GAACGTCTTG GAATGCT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

2019.09.20

GNP_filter3_RDBDEP_html_190816.pl