Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCGT-PDGFRA-C

Expression vector of human PDGFRA (deletion mutant with only the C-terminal portion), T7-tag.

Sequence (full)	RDB15465zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017）。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15465_B2Cgp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, HSV TK 5'-UTR, insert (FL) 5' Sequence file: RDB15465_B2Cga.seq
>D04855B3_B2Cg_1_CMV_Forward_A11_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCCN NNNNNNNNNN ATGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT   121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC GGGGGATCTT GGTGGCGTGA   181 AACTCCCGCA CCTCTTCGGC CAGCGCCTTG TAGAAGCGCG TATGGCTTCT AGGATGGCAT   241 CGATGACAGG TGGCCAACAG ATGGGTTCTA GGATGGCATC GATGACAGGT GGCCAACAGA   301 TGGGTTCTAG AGGATCTACT AGTCATATGG ATTGGTCTAG ACTGCCATAT GACTCAAGAT   361 GGGAGTTTCC AAGAGATGGA CTAGTGCTTG GTCGGGTCTT GGGGTCTGGA GCGTTTGGGA   421 AGGTGGTTGA AGGAACAGCC TATGGATTAA GCCGGTCCCA ACCTGTCATG AAAGTTGCAG   481 TGAAGATGCT AAAACCCACG GCCAGATCCA GTGAAAAACA AGCTCTCATG TCTGAACTGA   541 GGATAATGAC TCACCTGGGG CCACATTTGA ACATCGTAAA CTTGCTGGGA GCCTGCACCA   601 AGTCAGGCCC CATTTACATC ATCACAGAGT ATTACTTCTA TGGAGATTTG GTCAACTATT   661 TGCATAAGAA TAGGGATAGC TTCCTGAGCC ACCACCCAGA GAAGCCAAAG AAAGAGCTGG   721 ATATCTTTGG ACTGAACCCT GCTGATGAAA GCACACGGAG CTATGTTATT TTATCTTTTG   781 AAAACAATGA TGACTACATG GNCATGAAGC ANGCTGATAC TACACAGTAT GTCCCCATGC   841 TAGAAAGGAA AGAGGTTTCT AAATATTCCG ACATCCAGAG ATCACTCTAT GATCGTCCNG   901 CCTCATATAA GAAGAAATCT ATGTTAGANT CANAAGTCAA AAACCTNCTT NCNNNGNNNA   961 CTCNNANNNN TTACTTTATT GGNNTTGNTG ANCTNNNNCN NNCNNGTNGN CCNANNNNNN 1021 NNNTTTTTGN NTTCNAAAAN NGNGNNNNNN NNNN //
Primer: beta-gro_rev (Pr0512) Region: beta-globin pA, insert (FL) 3' Sequence file: RDB15465_B2Cgb.seq
>D04855B3_B2Cg_1_ beta-gro_rev_B09_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTGCCCCCT CNNTATAACA TGAATTTTAC NATAGCGAAA    61 AAGAAAGAAC AATCAAGGGT CCCCAAACTC ACCCTGAAGT TCTCAGGATC CGATTATCTC   121 GAGTTACAGG AAGCTGTCTT CCACCAGGTC TGAAGAGTCT ATGCCGATGT CGTCCATCAT   181 GTCGATGTCT TCAATGGTCT CGTCCTCTCT CTTGATGAAG GTGGAACTGC TGGAACCCGT   241 CTCAATGGCA CTCTCTTCAG AGGTCTGCGA GCTGTGTCTG TTCCTCTTGC CCAGGTCCTC   301 CTCCTCAGGG ACAGGGTCAA TGTCAGGCAG AGGAATGATG TAGCCACTGT CAGCGCTCAG   361 TCTCTGCTCA TCCAGACCAC CCTCCCAGTC CTTCAGCTTG TCTTCCTCGT TTTTGTAGGT   421 GACACCAATG TATGCATTGT CTGAGTCCAC ACGCATGCGT GCCACAGCAG GATGGTCACT   481 CTTCAGGAAG TCCAGGTGAA TTTTTTCATA ACTCTTTTTA TATTGTCCAG GCAGCAGATT   541 CTCCACAATC TCACTCAGGT GGTAAAAGGA GGGTCTCTTC TCCGGCTCAC TGTTCCAGCA   601 TTTCACCATG ATCTCGTAGA CTTCACTGGT AGCGTGGTCA GGCTTGGCCA TCCGGTACCC   661 ACTCTTGATC TTATTGNAGA AAGTAGAATC CACCATCATG CCGGGGTAAN GGGTGNCACC   721 NANGGAAAAG ATCTCCCANA NCANANNGNC NTAAGACCAG ACATCACTCA GTGTGGTGTA   781 NAGGNTGNCA AANANGCTCT CNNANCNNTC CNCTTCNCGG GCANAAAGGN ANTGNCTCCC   841 GANNCNTANT TCNAATCANG CNTGANGTCT CTGGNCNTGN CNAANTCNCN GANNNNCNCN   901 NTTTTTNNTT GNGNNNNNAN NANNNNNCNA GCANCNNNNT NNNGGNGNNN NNNNNTTTTT 961 TNANNNNAAA CNNCNNTNNN NNNGNNNNNN NNNN //
Primer: PDGFRA_F3 (Pr1185) Region: insert (FL) mid Sequence file: RDB15465_B2Cgc.seq
>D04855B3_B2Cg_1_PDGFRA_F3_F08_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNNNNNTTA NNTNNTCNCN GAGTATTACT TCTATGGAGA    61 TTTGGTCAAC TATTTGNNTA AGAATAGGGA TAGCTTCCTG AGCCACCACC CAGAGAAGCC   121 AAAGAAAGAG CTGGATATCT TTGGACTGAA CCCTGCTGAT GAAAGCACAC GGAGCTATGT   181 TATTTTATCT TTTGAAAACA ATGATGACTA CATGGACATG AAGCAGGCTG ATACTACACA   241 GTATGTCCCC ATGCTAGAAA GGAAAGAGGT TTCTAAATAT TCCGACATCC AGAGATCACT   301 CTATGATCGT CCAGCCTCAT ATAAGAAGAA ATCTATGTTA GACTCAGAAG TCAAAAACCT   361 CCTTTCAGGT GATAACTCAG AAGGCCTTAC TTTATTGGAT TTGTTGAGCT TCACCTATCA   421 AGTTGCCCGA GGAATGGAGT TTTTGGCTTC AAAAAATTGT GTCCACCGTG ATCTGGCTGC   481 TCGCAACGTC CTCCTGGCAC AAGGAAAAAT TGTGAAGATC TGTGACTTTG GCATGGCCAG   541 AGACATCATG CATGATTCGA ACTATGTGTC GGGAGGCAGT ACCTTTCTGC CCGTGAAGTG   601 NATGGCTCCT GAGAGCATCT TTGACAACCT CTACACCACA CTGAGTGATG TCTGGTCTTA   661 TGGCATTCTG CTCTGGGAGA NCTTTTCCCT TGNTGGCACC NCTTACCCCG GCATGATGGT   721 GGATTCTACT TTCTACAATA AGATCAAGAG TGGGTACCGG ATGGCCAAGC CTGACCACGC   781 TACCNGTGAA GTCTACNANA TCATGGTGAA ATGNTGGAAC AGTGANNCGG ANAANANANC   841 CTCCTTTTAC CNCNNGANNG ANANTGNGNA GAATCTGNTG NNNGNNNNNT ATAAAAANAG   901 TNATGAAAAA ATTCNCNNNN NNTNNNNGNA NANNGANNNT CCNGNTGNGN NNNNNNTGNN 961 NGNNNNNTCN NANNNGNATA CNTNNNNNNN NNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, beta-globin pA Sequence file: RDB15465_B2Cgd.seq
>D04855B3_B2Cg_1_ SV40pro_ori_F_C09_09_ABI24.ab1     1 NNNNNNNNNN NNNNCNNNNG CNNCCTCGNC CTCTGNNCTA TTCCNGAAGT AGTGAAGAGG    61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCTCGAG GGATCTCCAT AAGAGAAGAG   121 GGACAGCTAT GACTGGGAGT AGTCAGGAGA GGAGGAAAAA TCTGGCTAGT AAAACATGTA   181 AGGAAAATTT TAGGGATGTT AAAGAAAAAA ATAACACAAA ACAAAATATA AAAAAAATCT   241 AACCTCAAGT CAAGGCTTTT CTATGGAATA AGGAATGGAC AGCAGGGGGC TGTTTCATAT   301 ACTGATGACC TCTTTATAGC CAACCTTTGT TCATGGCAGC CAGCATATGG GCATATGTTG   361 CCAAACTCTA AACCAAATAC TCATTCTGAT GTTTTAAATG ATTTGCCCTC CCATATGTCC   421 TTCCGAGTGA GAGACACAAA AAATTCCAAC ACACTATTGC AATGAAAATA AATTTCCTTT   481 ATTAGCCAGA AGTCAGATGC TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG   541 GAAAAAGATC TCAGTGGTAT TTGTGAGCCA GGGCATTGGC CACACCAGCC ACCACCTTCT   601 GATAGGCAGC CTGCACCTGA NGAGTGAATT CTTTGCCAAA ATGATGAGAC AGCACAACAA   661 CCAGCACGTT GCCCAGGANC TGTANGAAAG AGAAGAANGN ATGAACATGG TTAGCAGANG   721 GGCCCGGTTT GNANTCNGAN TATTTTATCC TCATCTCNAA CAGTGTATAT CATTGTAACN   781 NTAAAGAGAA AGGNAGGANG ANGANNAGGG NGTAGTTGNT TCTANNNATN ANAANANTTC   841 CNCGCCNGCC NNAANTTATA TGCNNAAATA TTCTACCTTA TCATTTNNTT ATNNNNNTTG   901 NNCTCTAAAA CTGNGCTGNA NNANNNNATN NNATACCNNG ANNGNCNTNN NAAAAAAANN 961 GANNNNNNNA NGNANNNANN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl