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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCGT-FIP1L1-PDGFRA-FL (#RDB15463)

Expression vector of human FIP1L1-PDGFRA (full-length), T7-tag.

Clone info. Expression vector of human FIP1L1-PDGFRA (full-length), tagged with T7 at N-terminal.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pCGT (plasmid)
Selectable markers Amp^r
Gene/insert name human FIP1L1 cDNA human PDGFRA cDNA
Depositor|Developer Kondo, Takeshi |
 
Sequence (full) RDB15463zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017）。本件研究材料は、非営利機関の非営利学術研究に限って提供する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15463 pCGT-FIP1L1-PDGFRA-FL DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCGT-FIP1L1-PDGFRA-FL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15463).

Reference section:

Ibata, M., Iwasaki, J., Fujioka, Y., Nakagawa, K., Darmanin, S., Onozawa, M., Hashimoto, D., Ohba, Y., Hatakeyama, S., Teshima, T., Kondo, T., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15463_B2Cgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, HSV TK 5'-UTR, insert(FL) 5'
Sequence file: RDB15463_B2Cga.seq check
>D04855B1_B2Cg_3_ CMV_Forward_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTGNNNNNN NNGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT
  121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC GGGGGATCTT GGTGGCGTGA
  181 AACTCCCGCA CCTCTTCGGC CAGCGCCTTG TAGAAGCGCG TATGGCTTCT AGGATGGCAT
  241 CGATGACAGG TGGCCAACAG ATGGGTTCTA GAGCCATGTC GGCCGGCGAG GTCGAGCGCC
  301 TAGTGTCGGA GCTGAGCGGC GGGACCGGAG GGGATGAGGA GGAAGAGTGG CTCTATGGCG
  361 ATGAAAATGA AGTTGAAAGG CCAGAAGAAG AAAATGCCAG TGCTAATCCT CCATCTGGAA
  421 TTGAAGATGA AACTGCTGAA AATGGTGTAC CAAAACCGAA AGTGACTGAG ACCGAAGATG
  481 ATAGTGATAG TGACAGCGAT GATGATGAAG ATGATGTTCA TGTCACTATA GGAGACATTA
  541 AAACGGGAGC ACCACAGTAT GGGAGTTATG GTACAGCACC TGTAAATCTT AACATCAAGA
  601 CAGGGGGAAG AGTTTATGGA ACTACAGGGA CAAAAGTCAA AGGAGTAGAC CTTGATGCAC
  661 CTGGAAGCAT TAATGGAGTT CCACTCTTAG AGGTAGATTT GGATTCTTTT GAAGATAAAC
  721 CATGGCGTAA ACCTGGTGCT GATCTTTCTG ANTATTTTAA TTATGGGTTT AATGAAGATA
  781 CCTGGAAAGC TTACTGTGAA AAACAAAAGA NGATACGAAT GGGANTTGAA GTTATACCNG
  841 TAACNTCTAC TACAAATNAA NTTACNGNAC ANCNNNNANN AANNNGNAAA CTCAGANAAA
  901 NAANTGNCNN TCCATCTACA AAANCTGANT TTANTTCTNN TNNTTNTTNG TNNNNNANNN
961 NNTTCCNNNN AGCNNNANNN NNNNNNN
//
Primer: beta-gro_rev (Pr0512)
Region: beta-globin pA, insert(FL) 3'
Sequence file: RDB15463_B2Cgb.seq check
>D04855B1_B2Cg_3_beta-gro_rev_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTGCCCCCT CNNTATAACA TGAATTTTAC AATAGCGAAA
   61 AAGAAAGAAC AATCAAGGGT CCCCAAACTC ACCCTGAAGT TCTCAGGATC CATCAGGTCG
  121 AGTTACAGGA AGCTGTCTTC CACCAGGTCT GAAGAGTCTA TGCCGATGTC GTCCATCATG
  181 TCGATGTCTT CAATGGTCTC GTCCTCTCTC TTGATGAAGG TGGAACTGCT GGAACCCGTC
  241 TCAATGGCAC TCTCTTCAGA GGTCTGCGAG CTGTGTCTGT TCCTCTTGCC CAGGTCCTCC
  301 TCCTCAGGGA CAGGGTCAAT GTCAGGCAGA GGAATGATGT AGCCACTGTC AGCGCTCAGT
  361 CTCTGCTCAT CCAGACCACC CTCCCAGTCC TTCAGCTTGT CTTCCTCGTT TTTGTAGGTG
  421 ACACCAATGT ATGCATTGTC TGAGTCCACA CGCATGCGTG CCACAGCAGG ATGGTCACTC
  481 TTCAGGAAGT CCAGGTGAAT TTTTTCATAA CTCTTTTTAT ATTGTCCAGG CAGCAGANTC
  541 TCCACAATCT CACTCANGTG GTAAAAGGAG GGTCTCTTCT CCGGCTCACT GTTCCNGCAT
  601 TTCACCATGA TCTCGTANAC TTCACTGGNA NCGNGGTCNG GCTTGGCCNT CCNGNACCCN
661 CTCTTGNTCN NANNGNANAA NNNNNNNNNN
//
Primer: FIP1L1_F1 (Pr1075)
Region: insert mid (FL)
Sequence file: RDB15463_B2Cgc.seq check
>D04855B1_B2Cg_3_ FIP1L1_F1_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NTTGGNNTCT TTTGAAGANA AACCATGGCG
   61 TAAACCTGGT GCTGATCTTT CTGATTATTT TAATTATGGG TTTAATGAAG ATACCTGGAA
  121 AGCTTACTGT GAAAAACAAA AGAGGATACG AATGGGACTT GAAGTTATAC CAGTAACCTC
  181 TACTACAAAT AAAATTACGG TACAGCAGGG AAGAACTGGA AACTCAGAGA AAGAAACTGC
  241 CCTTCCATCT ACAAAAGCTG AGTTTACTTC TCCTCCTTCT TTGTTCAAGA CTGGGCTTCC
  301 ACCGAGCAGG AGATTACCTG GGGCAATTGA TGTTATCGGT CAGACTATAA CTATCAGCCG
  361 AGTAGAAGGC AGGCGACGGG CAAATGAGAA CAGCAACATA CAGCTGCCAT ATGACTCAAG
  421 ATGGGAGTTT CCAAGAGATG GACTAGTGCT TGGTCGGGTC TTGGGGTCTG GAGCGTTTGG
  481 GAAGGTGGTT GAAGGAACAG CCTATGGATT AAGCCGGTCC CAACCTGTCA TGAAAGTTGC
  541 AGTGAAGATG CTAAAACCCA CGGCCAGATC CAGTGAAAAA CAAGCTCTCA TGTCTGAACT
  601 GAGGATAATG ACTCACCTGG GGCCACATTT GAACATCGTA AACTTGCTGG GAGCCTGCAC
  661 CAAGTCAGGC CCCATTTACA TCATCACAGA GTATTGCTTC TATGGAGATT TGGTCAACTA
  721 TTTGCATAAG ANTAGGGATA GCTTCCTGAN CCACCACCCA GAGAANCCAA AGAAAGAGCT
  781 GGATATCTTT GGANTGAACC CTGCTGANGA AAGCACACGG ANCTATGTTA TTTTATCTTT
  841 TGAAAACNAT GGTGACTACN TGGNCNTGAA GCNNNTGATA CTACNCAGTA TGTCCCCNTG
  901 CTANAAAGGA AANANGTTTC TAAATATTCC NACNTNCNNA NNTNACTCTA NNATNGTNNN
  961 GCNTCNTNNN NNAANAANTC TATNTNANAN TNNNAANNNA AAANNNNNNT TNNNNGNNNA
1021 CTCNNNNNNN NNN
//
Primer: FIP1L1v3_F2 (Pr0515)
Region: insert mid (FL)
Sequence file: RDB15463_B2Cgd.seq check
>D04855B1_B2Cg_3_FIP1L1v3_F2_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNANGCGN NGGGCAANTG AGANCNGCAA CATACAGCTG
   61 CCATATGACT CAAGATGGGA GTTTCCAAGA GATGGACTAG TGCTTGGTCG GGTCTTGGGG
  121 TCTGGAGCGT TTGGGAAGGT GGTTGAAGGA ACAGCCTATG GATTAAGCCG GTCCCAACCT
  181 GTCATGAAAG TTGCAGTGAA GATGCTAAAA CCCACGGCCA GATCCAGTGA AAAACAAGCT
  241 CTCATGTCTG AACTGAGGAT AATGACTCAC CTGGGGCCAC ATTTGAACAT CGTAAACTTG
  301 CTGGGAGCCT GCACCAAGTC AGGCCCCATT TACATCATCA CAGAGTATTG CTTCTATGGA
  361 GATTTGGTCA ACTATTTGCA TAAGAATAGG GATAGCTTCC TGAGCCACCA CCCAGAGAAG
  421 CCAAAGAAAG AGCTGGATAT CTTTGGACTG AACCCTGCTG ATGAAAGCAC ACGGAGCTAT
  481 GTTATTTTAT CTTTTGAAAA CAATGGTGAC TACATGGACA TGAAGCAGGC TGATACTACA
  541 CAGTATGTCC CCATGCTAGA AAGGAAAGAG GTTTCTAAAT ATTCCGACAT CCAGAGATCA
  601 CTCTATGATC GTCCAGCCTC ATATAAGAAG AAATCTATGT TAGACTCAGA AGTCAAAAAC
  661 CTCCTTTCAG GTGATAACTC AGANNGNCTT ACTTTATTGN ATTTGNTGAG CTTCACCTAT
  721 CAAGTTGNNN GANGAATGGA GTTTTTGGCT TCNAAAAATT GTGTCCACCG TGATCTGGCT
  781 GCTCGCAACG NNCTCCTGNC ACAANGAAAA NTTGNGAANA TCTGTGACTT TGGCATNNNN
  841 NGANANNTCA TGCNTGANTC NAACTATGTG TCNGNNNNNA GTACNTTTCT GNCNNNGAAN
  901 NNNNNGGNTN NTGANANNNN NTTTGNNNNN CNCTACNCNN NNCTNANNGN NGNNNGNNNT
961 NNNNNNTNNN GNTCTNGGNN NNNNNNNTNN NNNNNNNN
//
Primer: PDGFRA_F1 (Pr1076)
Region: insert mid (FL)
Sequence file: RDB15463_B2Cge.seq check
>D04855B1_B2Cg_3_ PDGFRA_F1_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NNNCNNGANN NNNNNNAGGN TTCNNAAATA TTCCGACATC
   61 CNGAGATCAC TCTATGATCG TCCAGCCTCA TATAAGAAGA AATCTATGTT AGACTCAGAA
  121 GTCAAAAACC TCCTTTCAGG TGATAACTCA GAAGGCCTTA CTTTATTGGA TTTGTTGAGC
  181 TTCACCTATC AAGTTGCCCG AGGAATGGAG TTTTTGGCTT CAAAAAATTG TGTCCACCGT
  241 GATCTGGCTG CTCGCAACGT CCTCCTGGCA CAAGGAAAAA TTGTGAAGAT CTGTGACTTT
  301 GGCATGGCCA GAGACATCAT GCATGATTCG AACTATGTGT CGGAAGGCAG TACCTTTCTG
  361 CCCGTGAAGT GGATGGCTCC TGAGAGCATC TTTGACAACC TCTACACCAC ACTGAGTGAT
  421 GTCTGGTCTT ATGGCATTCT GCTCTGGGAG ATCTTTTCCC TTGGTGGCAC CCCTTACCCC
  481 GGCATGATGG TGGATTCTAC TTTCTACAAT AAGATCAAGA GTGGGTACCG GATGGCCAAG
  541 CCTGANNNNN CTACCAGTGA AGTCTACGAG ATCATGGTGA AATGCTGGAA CAGTGAGCCG
  601 GAGAAGAGAC CCTCCTTTTA CCACCTGAGT GAGATTGTGG AGAATCTGCT GCCTGGACAA
  661 TATAAAAAGA GTTATGAAAA AATTCACCTG GACTTCCTGA AGAGTGACCN TCCTGCTGTG
  721 GCACGCATGC GTGTGGACTC ANACNATGCA TACNTTGGTG TCACCTANAA AAACGANGAA
  781 NANNAGCTGA NNGACTGGGN NGGNGGTCTG GATGANCANA NACTGAGCGC TGACNGTGNC
  841 TACNTCATTC CTCTGNCTGA CNNTNACCCN GNNCCTGANG ANGNNGNNNN NNNNANNANN
  901 AACANNNNCN GCTCGCANNN CTCTGAANAN ANNGNCNTTG NNANNGNTTN NANCANTTNN
961 NNCNTNNNTN AANAANANAN GACNANACNN TTGAANANNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,beta-globin pA
Sequence file: RDB15463_B2Cgf.seq check
>D04855B1_B2Cg_3_ SV40pro_ori_F_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNNNN NNGCCTCNNC CTCTGANCTA TTCCNGAAGT AGTGAAGAGG
   61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCTCGAG GGATCTCCAT AAGAGAAGAG
  121 GGACAGCTAT GACTGGGAGT AGTCAGGAGA GGAGGAAAAA TCTGGCTAGT AAAACATGTA
  181 AGGAAAATTT TAGGGATGTT AAAGAAAAAA ATAACACAAA ACAAAATATA AAAAAAATCT
  241 AACCTCAAGT CAAGGCTTTT CTATGGAATA AGGAATGGAC AGCAGGGGGC TGTTTCATAT
  301 ACTGATGACC TCTTTATAGC CAACCTTTGT TCATGGCAGC CAGCATATGG GCATATGTTG
  361 CCAAACTCTA AACCAAATAC TCATTCTGAT GTTTTAAATG ATTTGCCCTC CCATATGTCC
  421 TTCCGAGTGA GAGACACAAA AAATTCCAAC ACACTATTGC AATGAAAATA AATTTCCTTT
  481 ATTAGCCAGA AGTCAGATGC TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG
  541 GAAAAAGATC TCAGTGGTAT TTGTGAGCCA GGGCATTGGC CACACCAGCC ACCACCTTCT
  601 GATAGGCAGC CTGCACCTGA NGAGTGAATT CTTTGCCAAA ATGATGAGAC AGCACAACAA
  661 CCAGCACGTT GCCCAGGANC TGTAGGAAAG AGAAGAAGGC ATGAACATGG TTAGCANAGG
  721 GGCCCGGNTT GGACTCAGAG TATTTTATCC TCATCTCAAA CAGTGTATAT CATTGTAACC
  781 ATAAAGAGAA ANGNANGANG ANGACCAGGG NGTANNTGNT TCTANCAATA ANANNANTTC
  841 CNCGCCNGCC NGAATTTATA TGCNNAAATA TTCTACCTTA TCNNTNNNNT NTNNCNANTG
  901 NNCTCTAAAA NTGTGCTGAN NTANANTATN NTATACCNNG ANNGNNNNNN AAAAAAANNN
961 ANNNNNNAAN GTANNTNNNN CTGANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ibata, M., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034. [link to RRC of NBRP]
reference Iwasaki, J., FIP1L1 presence in FIP1L1-RARA or FIP1L1-PDGFRA differentially contributes to the pathogenesis of distinct types of leukemia. Ann. Hematol. 93 (9): 1473-1481 (2014). PMID 24763514.