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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFLAG-FIP1L1-PDGFRA-FL (#RDB15460)

Expression vector of human FIP1L1-PDGFRA (full-length), FLAG-tag.

Clone info. Expression vector of human FIP1L1-PDGFRA (full-length), tagged with FLAG at N-terminal.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pFLAG-CMV (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human FIP1L1 cDNA human PDGFRA cDNA
Depositor|Developer Kondo, Takeshi |
 
Sequence (full) RDB15460zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017）。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15460 pFLAG-FIP1L1-PDGFRA-FL DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-FIP1L1-PDGFRA-FL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15460).

Reference section:

Ibata, M., Iwasaki, J., Fujioka, Y., Nakagawa, K., Darmanin, S., Onozawa, M., Hashimoto, D., Ohba, Y., Hatakeyama, S., Teshima, T., Kondo, T., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15460_B2Cgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Sequence file: RDB15460_B2Cga.seq check
>D04855A7_B2Cg_3_ CMV_Forward_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNGCNAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCGTTTAG TGAACCGTCA GAATTAATTC AGATCGATCT ACCATGGACT
  121 ACAAAGACGA TGACGACAAG CTTGCGGCCG CGAATTCATC GATAGATCTG ATATCGGTAC
  181 CAGTCGACTC TAGAGCCATG TCGGCCGGCG AGGTCGAGCG CCTAGTGTCG GAGCTGAGCG
  241 GCGGGACCGG AGGGGATGAG GAGGAAGAGT GGCTCTATGG CGATGAAAAT GAAGTTGAAA
  301 GGCCAGAAGA AGAAAATGCC AGTGCTAATC CTCCATCTGG AATTGAAGAT GAAACTGCTG
  361 AAAATGGTGT ACCAAAACCG AAAGTGACTG AGACCGAAGA TGATAGTGAT AGTGACAGCG
  421 ATGATGATGA AGATGATGTT CATGTCACTA TAGGAGACAT TAAAACGGGA GCACCACAGT
  481 ATGGGAGTTA TGGTACAGCA CCTGTAAATC TTAACATCAA GACAGGGGGA AGAGTTTATG
  541 GAACTACAGG GACAAAAGTC AAAGGAGTAG ACCTTGATGC ACCTGGAAGC ATTAATGGAG
  601 TTCCACTCTT AGAGGTAGAT TTGGATTCTT TTGAAGATAA ACCATGGCGT AAACCTGGTG
  661 CTGATCTTTC TGATTATTTT AATTATGGGT TTAATGAAGA TACCTGGAAA GCTTACTGTG
  721 AAAAACAAAA GAGGANACGA ATGGGACTTG AAGTTATACC AGTAACCTCT ACTACNAATA
  781 AAATTACNGT ACAGCANGGA AGANNTGGAA ACTCANANAA AGAAACTGNC CTTCCNTCTA
  841 CAAAAGCTGA NTTNACTTCT CCTNCTTCTT TGTTCNANAN TGGGCTTCCN CCNANCNNNA
  901 NANTACNNGG GGNNATNGAT GNNNNCGGNC ANANTNTNAC TATCANCCNA NTANANNNNN
961 NNNGANNGNN NANTGANNNN NGCNNCNTAC NGCTGNNNNN NNNNN
//
Primer: PDGFRA_F2
Sequence file: RDB15460_B2Cgb.seq check
>D04855A7_B2Cg_3_ PDGFRA_F2_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CCTCCTTTTA CCNCCNNGAG TGANNNNNNN
   61 NAGAATCTNG CTGCCNNGGA CAATATAAAA AGAGTTATGA AAAAATTCAC CTGGACTTCC
  121 TGAAGAGTGA CCATCCTGCT GTGGCACGCA TGCGTGTGGA CTCAGACAAT GCATACATTG
  181 GTGTCACCTA CAAAAACGAG GAAGACAAGC TGAAGGACTG GGAGGGTGGT CTGGATGAGC
  241 AGAGACTGAG CGCTGACAGT GGCTACATCA TTCCTCTGCC TGACATTGAC CCTGTCCCTG
  301 AGGAGGAGGA CCTGGGCAAG AGGAACAGAC ACAGCTCGCA GACCTCTGAA GAGAGTGCCA
  361 TTGAGACGGG TTCCAGCAGT TCCACCTTCA TCAAGAGAGA GGACGAGACC ATTGAAGACA
  421 TCGACATGAT GGACGACATC GGCATAGACT CTTCAGACCT GGTGGAAGAC AGCTTCCTGT
  481 AACTCGACCT GATGGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG
  541 CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA
  601 TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA
  661 AGGGGCAAGT TGGGAAGACA ACCTGTAGGG NCTGCGGGGT CTATTGGGAA NNNAGCTGGA
  721 GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGNNTTCAA GCGATTCTCC
  781 TGCCTCAGCC TCCCGAGTTG TTGGGANTCC AGGNATGCNT GANNNNGCTC ANCTAANTTT
  841 TGTTTTTTTG GNANANNNGG GGTTTCACCA TATTGGCNNN GCTNNNCTCN NACTNCNNAN
  901 CTCNNGNGAN NNACCCANCT NGNNNNCCCN AANNGNNGGN NTNCNNGNNT GNNCNANNGC
961 TCCNNNCCNN TNNTTNNNAN TTNAAANNAN NNNNNNNNN
//
Primer: FIP1L1_F1
Sequence file: RDB15460_B2Cgc.seq check
>D04855A7_B2Cg_3_ FIP1L1_F1_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNNNTCTT TTGNNGNNAA ACCATGGCGT
   61 AAACCTGGTG CTGATCTTTC TGATTATTTT AATTATGGGT TTAATGAAGA TACCTGGAAA
  121 GCTTACTGTG AAAAACAAAA GAGGATACGA ATGGGACTTG AAGTTATACC AGTAACCTCT
  181 ACTACAAATA AAATTACGGT ACAGCAGGGA AGAACTGGAA ACTCAGAGAA AGAAACTGCC
  241 CTTCCATCTA CAAAAGCTGA GTTTACTTCT CCTCCTTCTT TGTTCAAGAC TGGGCTTCCA
  301 CCGAGCAGGA GATTACCTGG GGCAATTGAT GTTATCGGTC AGACTATAAC TATCAGCCGA
  361 GTAGAAGGCA GGCGACGGGC AAATGAGAAC AGCAACATAC AGCTGCCATA TGACTCAAGA
  421 TGGGAGTTTC CAAGAGATGG ACTAGTGCTT GGTCGGGTCT TGGGGTCTGG AGCGTTTGGG
  481 AAGGTGGTTG AAGGAACAGC CTATGGATTA AGCCGGTCCC AACCTGTCAT GAAAGTTGCA
  541 GTGAAGATGC TAAAACCCAC GGCCAGATCC AGTGAAAAAC AAGCTCTCAT GTCTGAACTG
  601 AGGATAATGA CTCACCTGGG GCCACATTTG AACATCGTAA ACTTGCTGGG AGCCTGCACC
  661 AAGTCAGGCC CCATTTACAT CATCACAGAG TATTGCTTCT ATGGAGATTT GGTCAACTAT
  721 TTGCATAAGA ATAGGGANAG CTTCCTGAGC CANCACCCAG AGAANCCAAA GAAAGAGCTG
  781 GATATCTTTG GACTGAACCC TGCTGATGAA AGCACACGGA NCTATGTTAT TTTATCTTTT
  841 GAAAACAATG GTGANTACNT GGACNTGAAG CNNNTGATAC TACNCANNAT GNNCCCNNGC
  901 TANAAAGGAA ANANGNTNCT AAANATTCCN ANNTNCNNAN NTNANTCNAT GATCGTCCNN
  961 CNTCNTNNNN ANNAANTCTA TGNNANNNTN NNNANTCNAA ANNNNNNNTT CNNNNGNNNN
1021 NNNNNN
//
Primer: FIP1L1v3_F2
Sequence file: RDB15460_B2Cgd.seq check
>D04855A7_B2Cg_3_FIP1L1v3_F2_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCGNNNGNNN NNNAGAACNG CAACATACAG CTGCCATATG
   61 ACTCAAGATG GGAGTTTCCA AGAGATGGAC TAGTGCTTGG TCGGGTCTTG GGGTCTGGAG
  121 CGTTTGGGAA GGTGGTTGAA GGAACAGCCT ATGGATTAAG CCGGTCCCAA CCTGTCATGA
  181 AAGTTGCAGT GAAGATGCTA AAACCCACGG CCAGATCCAG TGAAAAACAA GCTCTCATGT
  241 CTGAACTGAG GATAATGACT CACCTGGGGC CACATTTGAA CATCGTAAAC TTGCTGGGAG
  301 CCTGCACCAA GTCAGGCCCC ATTTACATCA TCACAGAGTA TTGCTTCTAT GGAGATTTGG
  361 TCAACTATTT GCATAAGAAT AGGGATAGCT TCCTGAGCCA CCACCCAGAG AAGCCAAAGA
  421 AAGAGCTGGA TATCTTTGGA CTGAACCCTG CTGATGAAAG CACACGGAGC TATGTTATTT
  481 TATCTTTTGA AAACAATGGT GACTACATGG ACATGAAGCA GGCTGATACT ACACAGTATG
  541 TCCCCATGCT AGAAAGGAAA GAGGTTTCTA AATATTCCGA CATCCAGAGA TCACTCTATG
  601 ATCGTCCAGC CTCATATAAG AAGAAATCTA TGTTAGACTC AGAAGTCAAA AACCTCCTTT
  661 CANGTGATAA CTCAGAANGN CTTACTTTAT TGGATTTGTT GAGCTTCNCC TATCAAGTTG
  721 CCCGAGGAAT GGAGTTTTTG GCTTCNAAAA NTTGTGTCCA CCGNGATCTG GCTGCTCGCA
  781 ACGTCCTCCT GGCACAAGGA AAAANTGTGA ANANCTGTGA NTTTNNNATN NNNNGANANA
  841 TCATGNNTGA NTCGAANTAT NNGNCNNNAN NNAGTANCTT TCTGNCNNNG AANTGNNNGG
  901 NTNNNGNNAN CNTNTTNNNN NNNNNCNACN NNNCNCNNNN NGNNGNNNGG NNTNANNNNN
961 NNNNN
//
Primer: PDGFRA_F1
Sequence file: RDB15460_B2Cge.seq check
>D04855A7_B2Cg_3_ PDGFRA_F1_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNGAAGGNN NAGGNTTCNN AAATATTCCG ACATCCAGAG
   61 ATCACTCTAT GATCGTCCAG CCTCATATAA GAAGAAATCT ATGTTAGACT CAGAAGTCAA
  121 AAACCTCCTT TCAGGTGATA ACTCAGAAGG CCTTACTTTA TTGGATTTGT TGAGCTTCAC
  181 CTATCAAGTT GCCCGAGGAA TGGAGTTTTT GGCTTCAAAA AATTGTGTCC ACCGTGATCT
  241 GGCTGCTCGC AACGTCCTCC TGGCACAAGG AAAAATTGTG AAGATCTGTG ACTTTGGCAT
  301 GGCCAGAGAC ATCATGCATG ATTCGAACTA TGTGTCGGAA GGCAGTACCT TTCTGCCCGT
  361 GAAGTGGATG GCTCCTGAGA GCATCTTTGA CAACCTCTAC ACCACACTGA GTGATGTCTG
  421 GTCTTATGGC ATTCTGCTCT GGGAGATCTT TTCCCTTGGT GGCACCCCTT ACCCCGGCAT
  481 GATGGTGGAT TCTACTTTCT ACAATAAGAT CAAGAGTGGG TACCGGATGG CCAAGCCTGA
  541 CCACGCTACC AGTGAAGTCT ACGAGATCAT GGTGAAATGC TGGAACAGTG AGCCGGAGAA
  601 GAGACCCTCC TTTTACCACC TGAGTGAGAT TGTGGAGAAT CTGCTGCCTG GACAATATAA
  661 AAAGAGTTAT GAAAAAATTC ACCTGGACTT CCTGAAGAGT GACCATCCTG CTGTGGCACG
  721 CATGCGTGTG GACTCAGACA ATGCATACAT TGGTGTCACC TACNAAAACG AGGAANANAA
  781 GCTGAANGAN TGNNNNGGTG GTCTGGATGA NCAGANANTG ANCGCTGANA GTGNCTACNT
  841 CATTCCTCTG NCTGANNNTG ANCCNGNCCC NGANGANGAN GANNNGGGCA AGANGAACAN
  901 NNNCNNCTCG CANANCTCTG AANANAGTGN CNTNGANANN GNNNCCNNCA NTNCCNNCTT
961 CNTCNNNANA NANNNNNANA NNNTTNNNNA NNTCNNNNTG ANNNNNNANN NNNNNNNN
//
Primer: SV40pro_ori_F
Sequence file: RDB15460_B2Cgf.seq check
>D04855A7_B2Cg_3_ SV40pro_ori_F_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNG NCNCCTCGGC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG
   61 CTTTTTTGGA GGAATTGATC AGCTTGGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC
  121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
  181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
  241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
  301 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
  361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
  421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
  481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
  541 GCATCGAGCG AGCACGTACT CNNNTGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
  601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG
  661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
  721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGN TGTGGCGGAN CGCTATCAGG
  781 ACATAGCGTT GGCTACCCGT GATATTGCTG AANAGCTNGN CGGCGAATGG GCTGANCGCT
  841 TCCNCGNGNT TTACNGNATC GCCGCTNCCN ATTCNNANCG NATCNCCNTC TATCNNCNNN
  901 NNGANNANTN CTTCTGANNG GNNNTCNGGG GNNCNAAATG ANNGANCNAN CNNNNNCCNN
961 NCNNNNNNTN NNNANATTTC NANTCCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ibata, M., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034. [link to RRC of NBRP]
reference Iwasaki, J., FIP1L1 presence in FIP1L1-RARA or FIP1L1-PDGFRA differentially contributes to the pathogenesis of distinct types of leukemia. Ann. Hematol. 93 (9): 1473-1481 (2014). PMID 24763514.