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pcDNA3.1(+)-c.C225R (#RDB15440)

Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225R missense mutation.

Clone info. Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225R (c.637T>A) missense mutation. Total RNA was extracted from mouse embryos at embryonic day 18.5.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Tgfb1 cDNA
Depositor|Developer Furuichi, Tatsuya |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017）。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15440 pcDNA3.1(+)-c.C225R DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(+)-c.C225R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15440).

Reference section:

Ichimura, S., Sasaki, S., Murata, T., Fukumura, R., Gondo, Y., Ikegawa, S., Furuichi, T., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15440_A7Jgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB15440_A7Jga.seq check
>D04886A3_A7Jg_1_CMV-Forward_G03_21_ABI24.ab1
    1 CTTACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTGGTACC GAGCTCGGAT
  181 CCACTAGTCC AGTGTGGTGG AATTCATCCC ACCTTTGCCG AGGGTTCCCG CTCTCCGAAG
  241 TGCCGTGGGG CGCCGCCTCC CCCATGCCGC CCTCGGGGCT GCGGCTACTG CCGCTTCTGC
  301 TCCCACTCCC GTGGCTTCTA GTGCTGACGC CCGGGAGGCC AGCCGCGGGA CTCTCCACCT
  361 GCAAGACCAT CGACATGGAG CTGGTGAAAC GGAAGCGCAT CGAAGCCATC CGTGGCCAGA
  421 TCCTGTCCAA ACTAAGGCTC GCCAGTCCCC CAAGCCAGGG GGAGGTACCG CCCGGCCCGC
  481 TGCCCGAGGC GGTGCTCGCT TTGTACAACA GCACCCGCGA CCGGGTGGCA GGCGAGAGCG
  541 CCGACCCAGA GCCGGAGCCC GAAGCGGACT ACTATGCTAA AGAGGTCACC CGCGTGCTAA
  601 TGGTGGACCG CAACAACGCC ATCTATGAGA AAACCAAAGA CATCTCACAC AGTATATATA
  661 TGTTCTTCAA TACGTCAGAC ATTCGGGAAG CAGTGCCCGA ACCCCCATTG CTGTCCCGTG
  721 CAGAGCTGCG CTTGCAGAGA TTAAAATCAA GTGTGGAGCA ACATGTGGAA CTCTACCAGA
  781 AATATAGCAA CAATTCCTGG CGTTACCTTG GTAACCGGCT GCTGACCCCC ACTGATACGC
  841 CTGAGTGGCT GTCTTTTGAC GTCACTGGAG TTGTACGGCA GTGGCTGAAC CAAGGAGACG
  901 GAAATACAGG GCTTTCGATT CAGCGCTCAC TGCTCTCGTG ACAGCAAAGA TAACAACTCC
  961 ACGTGGTAAA TCAACGGGGA TCAGCCCCCA AACGTCTGGG CGACCCTGGG GCACCATCCC
 1021 ATGACATGAA CCCGGCCCTT CCTGCCTCAT CATTGACCAC CCCCCCGTGG CAAGGACCAA
1081 GCAACTTGCA CAAGCTCCAC GGCCAAACCC
//
Primer: BGH_rev2 ()
Sequence file: RDB15440_A7Jgb.seq check
>D04886A3_A7Jg_1_BGH_rev2_H03_24_ABI24.ab1
    1 CATCGTTAGA GCACGGGGGA GGGGCAACAA CAGATGGCTG GCAACTAGAA GGCACAGTCG
   61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCTCA GCTGCACTTG
  121 CAGGAGCGCA CAATCATGTT GGACAACTGC TCCACCTTGG GCTTGCGACC CACGTAGTAG
  181 ACGATGGGCA GTGGCTCCAA AGCCTGCGGC ACGCAGCACG GTGACGCCGA AGCGCCCGGG
  241 TTGTGTTGGT TGTAGAGGGC AAGGACCTTG CTGTACTGTG TGTCCAGGCT CCAAATATAG
  301 GGGCAGGGTC CCAGACAGAA GTTGGCATGG TAGCCCTTGG GCTCGTGGAT CCACTTCCAA
  361 CCCAGGTCCT TCCTAAAGTC AATGTACAGC TGCCGCACAC AGCAGTTCTT CTCTGTGGAG
  421 CTGAAGCAAT AGTTGGTATC CAGGGCTCTC CGGTGCCGTG AGCTGTGCAG GTGCTGGGCC
  481 CTTTCCAGGG GGGTGGCCAT GAGGAGCAGG AAGGGCCGGT TCATGTCATG GATGGTGCCC
  541 AGGTCGCCCC GACGTTTGGG GCTGATCCCG TTGATTTCCA CGTGGAGTTT GTTATCTTTG
  601 CTGTCACGAG AGCAGTGAGC GCTGAATCGA AAGCCCTGTA TTCCGTCTCC TTGGTTCAGC
  661 CACTGCCGTA CAACTCCAGT GACGTCAAAA GACAGCCACT CAGGCGTATC AGTGGGGGTC
  721 AGCAGCCGGT TACCAAGGTA ACGCCAGGAA TTGTTGCTAT ATTTCTGGTA GAGTTCCACA
  781 TGTTGCTCCA CACTTGATTT TAATCTCTGC AAGCGCAGCT CTGCACGGGA CAGCAATGGG
  841 GGTTCGGGCA CTGCTTCCCG AATGTCTGAC GTATTGAAGA ACATATATAT ACTGTGTGAG
  901 ATGTCTTTGG TTTTCTCATA GATGGCGTTG TTGCGGTCCA CCATTAGCAC GCGGGTGACC
  961 CTCTTTAGCA TAGTAGTCCG CTTTCGGGCT CCGGGCTCTG GGTCGGGCGC TCTCGCCTGG
 1021 CCACCCGGTC GCGGTGCTGT TGTACCAAGC CGAGCAACGC TCGGGCAGCG GTCGGTCGTT
1081 AGTCCCCCTT GACTTGGGGG GAACTGACCT GA
//
Primer: SV40pro_F ()
Sequence file: RDB15440_A7Jgc.seq check
>D04886A3_A7Jg_1_SV40pro_F_H02_23_ABI24.ab1
    1 GCTGGTCATT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
  961 CCAACCCTGC CCATCACGAG ATTTCGATTC CACCGCCGCC TTTCTATGAA AGGTTGGGCT
 1021 TCGGAATCGG TTTTTCCGGG ACGCCGGCTG GATGAATCTT CAGCGCGGGG ATTCTTCATG
1081 CTGCAGTCCA TCGCCCAACC CCGACTTGCA TTATTTGCCA G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ichimura, S., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112. [link to RRC of NBRP]