RDB15440 - pcDNA3.1(+)-c.C225R

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pcDNA3.1(+)-c.C225R

Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225R missense mutation.

Catalog number	RDB15440
Resource name	pcDNA3.1(+)-c.C225R
Clone info.	Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225R (c.637T>A) missense mutation. Total RNA was extracted from mouse embryos at embryonic day 18.5.
Comment	Expression was confirmed by the depositor with western blotting.
Vector backbone	pcDNA3.1(+) (plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	mouse Tgfb1 cDNA
Depositor\|Developer	Furuichi, Tatsuya \|

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mouse Tgfb1

External Database
mouse Tgfb1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017).

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017）。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15440	pcDNA3.1(+)-c.C225R	DNA solution

Please review the QC test results indicated by check

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References and tips

Electronic file

Original reference

original	Ichimura, S., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15440_A7Jgp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB15440_A7Jga.seq
>D04886A3_A7Jg_1_CMV-Forward_G03_21_ABI24.ab1 1 CTTACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC 61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT 121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTGGTACC GAGCTCGGAT 181 CCACTAGTCC AGTGTGGTGG AATTCATCCC ACCTTTGCCG AGGGTTCCCG CTCTCCGAAG 241 TGCCGTGGGG CGCCGCCTCC CCCATGCCGC CCTCGGGGCT GCGGCTACTG CCGCTTCTGC 301 TCCCACTCCC GTGGCTTCTA GTGCTGACGC CCGGGAGGCC AGCCGCGGGA CTCTCCACCT 361 GCAAGACCAT CGACATGGAG CTGGTGAAAC GGAAGCGCAT CGAAGCCATC CGTGGCCAGA 421 TCCTGTCCAA ACTAAGGCTC GCCAGTCCCC CAAGCCAGGG GGAGGTACCG CCCGGCCCGC 481 TGCCCGAGGC GGTGCTCGCT TTGTACAACA GCACCCGCGA CCGGGTGGCA GGCGAGAGCG 541 CCGACCCAGA GCCGGAGCCC GAAGCGGACT ACTATGCTAA AGAGGTCACC CGCGTGCTAA 601 TGGTGGACCG CAACAACGCC ATCTATGAGA AAACCAAAGA CATCTCACAC AGTATATATA 661 TGTTCTTCAA TACGTCAGAC ATTCGGGAAG CAGTGCCCGA ACCCCCATTG CTGTCCCGTG 721 CAGAGCTGCG CTTGCAGAGA TTAAAATCAA GTGTGGAGCA ACATGTGGAA CTCTACCAGA 781 AATATAGCAA CAATTCCTGG CGTTACCTTG GTAACCGGCT GCTGACCCCC ACTGATACGC 841 CTGAGTGGCT GTCTTTTGAC GTCACTGGAG TTGTACGGCA GTGGCTGAAC CAAGGAGACG 901 GAAATACAGG GCTTTCGATT CAGCGCTCAC TGCTCTCGTG ACAGCAAAGA TAACAACTCC 961 ACGTGGTAAA TCAACGGGGA TCAGCCCCCA AACGTCTGGG CGACCCTGGG GCACCATCCC 1021 ATGACATGAA CCCGGCCCTT CCTGCCTCAT CATTGACCAC CCCCCCGTGG CAAGGACCAA 1081 GCAACTTGCA CAAGCTCCAC GGCCAAACCC //
Primer: BGH_rev2 Sequence file: RDB15440_A7Jgb.seq
>D04886A3_A7Jg_1_BGH_rev2_H03_24_ABI24.ab1 1 CATCGTTAGA GCACGGGGGA GGGGCAACAA CAGATGGCTG GCAACTAGAA GGCACAGTCG 61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCTCA GCTGCACTTG 121 CAGGAGCGCA CAATCATGTT GGACAACTGC TCCACCTTGG GCTTGCGACC CACGTAGTAG 181 ACGATGGGCA GTGGCTCCAA AGCCTGCGGC ACGCAGCACG GTGACGCCGA AGCGCCCGGG 241 TTGTGTTGGT TGTAGAGGGC AAGGACCTTG CTGTACTGTG TGTCCAGGCT CCAAATATAG 301 GGGCAGGGTC CCAGACAGAA GTTGGCATGG TAGCCCTTGG GCTCGTGGAT CCACTTCCAA 361 CCCAGGTCCT TCCTAAAGTC AATGTACAGC TGCCGCACAC AGCAGTTCTT CTCTGTGGAG 421 CTGAAGCAAT AGTTGGTATC CAGGGCTCTC CGGTGCCGTG AGCTGTGCAG GTGCTGGGCC 481 CTTTCCAGGG GGGTGGCCAT GAGGAGCAGG AAGGGCCGGT TCATGTCATG GATGGTGCCC 541 AGGTCGCCCC GACGTTTGGG GCTGATCCCG TTGATTTCCA CGTGGAGTTT GTTATCTTTG 601 CTGTCACGAG AGCAGTGAGC GCTGAATCGA AAGCCCTGTA TTCCGTCTCC TTGGTTCAGC 661 CACTGCCGTA CAACTCCAGT GACGTCAAAA GACAGCCACT CAGGCGTATC AGTGGGGGTC 721 AGCAGCCGGT TACCAAGGTA ACGCCAGGAA TTGTTGCTAT ATTTCTGGTA GAGTTCCACA 781 TGTTGCTCCA CACTTGATTT TAATCTCTGC AAGCGCAGCT CTGCACGGGA CAGCAATGGG 841 GGTTCGGGCA CTGCTTCCCG AATGTCTGAC GTATTGAAGA ACATATATAT ACTGTGTGAG 901 ATGTCTTTGG TTTTCTCATA GATGGCGTTG TTGCGGTCCA CCATTAGCAC GCGGGTGACC 961 CTCTTTAGCA TAGTAGTCCG CTTTCGGGCT CCGGGCTCTG GGTCGGGCGC TCTCGCCTGG 1021 CCACCCGGTC GCGGTGCTGT TGTACCAAGC CGAGCAACGC TCGGGCAGCG GTCGGTCGTT 1081 AGTCCCCCTT GACTTGGGGG GAACTGACCT GA //
Primer: SV40pro_F Sequence file: RDB15440_A7Jgc.seq
>D04886A3_A7Jg_1_SV40pro_F_H02_23_ABI24.ab1 1 GCTGGTCATT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT 121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC 661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC 961 CCAACCCTGC CCATCACGAG ATTTCGATTC CACCGCCGCC TTTCTATGAA AGGTTGGGCT 1021 TCGGAATCGG TTTTTCCGGG ACGCCGGCTG GATGAATCTT CAGCGCGGGG ATTCTTCATG 1081 CTGCAGTCCA TCGCCCAACC CCGACTTGCA TTATTTGCCA G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Ichimura, S., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl