RDB15439 - pcDNA3.1(+)-c.C225S

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pcDNA3.1(+)-c.C225S

Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225S missense mutation.

Catalog number	RDB15439
Resource name	pcDNA3.1(+)-c.C225S
Clone info.	Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225S (c.637T>A) missense mutation. Total RNA was extracted from mouse embryos at embryonic day 18.5.
Comment	Expression was confirmed by the depositor with western blotting.
Vector backbone	pcDNA3.1(+) (plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	mouse Tgfb1 cDNA
Depositor\|Developer	Furuichi, Tatsuya \|

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mouse Tgfb1

External Database
mouse Tgfb1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017).

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017）。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15439	pcDNA3.1(+)-c.C225S	DNA solution

Please review the QC test results indicated by check

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References and tips

Electronic file

Original reference

original	Ichimura, S., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15439_A7Jgp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB15439_A7Jga.seq
>D04886A2_A7Jg_1_CMV-Forward_D03_12_ABI24.ab1 1 CGTTCCTCCG GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG 121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG 181 GATCCACTAG TCCAGTGTGG TGGAATTCAT CCCACCTTTG CCGAGGGTTC CCGCTCTCCG 241 AAGTGCCGTG GGGCGCCGCC TCCCCCATGC CGCCCTCGGG GCTGCGGCTA CTGCCGCTTC 301 TGCTCCCACT CCCGTGGCTT CTAGTGCTGA CGCCCGGGAG GCCAGCCGCG GGACTCTCCA 361 CCTGCAAGAC CATCGACATG GAGCTGGTGA AACGGAAGCG CATCGAAGCC ATCCGTGGCC 421 AGATCCTGTC CAAACTAAGG CTCGCCAGTC CCCCAAGCCA GGGGGAGGTA CCGCCCGGCC 481 CGCTGCCCGA GGCGGTGCTC GCTTTGTACA ACAGCACCCG CGACCGGGTG GCAGGCGAGA 541 GCGCCGACCC AGAGCCGGAG CCCGAAGCGG ACTACTATGC TAAAGAGGTC ACCCGCGTGC 601 TAATGGTGGA CCGCAACAAC GCCATCTATG AGAAAACCAA AGACATCTCA CACAGTATAT 661 ATATGTTCTT CAATACGTCA GACATTCGGG AAGCAGTGCC CGAACCCCCA TTGCTGTCCC 721 GTGCAGAGCT GCGCTTGCAG AGATTAAAAT CAAGTGTGGA GCAACATGTG GAACTCTACC 781 AGAAATATAG CAACAATTCC TGGCGTTACC TTGGTAACCG GCTGCTGACC CCCACTGATA 841 CGCCTGAGTG GCTGTCTTTT GACGTCACTG GAGTTGTACG GCAGTGGCTG AACCAAGGAG 901 ACGGAATACA GGGCTTTCGA TTCAGCGCTC ACTGCTCTAG TGACAGCAAA GATAACAAAC 961 TCCACGTGGT AATCAACGGG ATCAGCCCCC AAACGTCGGG GCGACCCTGC GCACCATCCC 1021 ATGACATGAA CCGGGCCCTT TCCTGCTTCA TCATTGACAA CCCCCCTGCA AAGGACCAGC 1081 ACTTGCACAG CTCACGGCAC CGGGAGAAGT TC //
Primer: BGH_rev2 Sequence file: RDB15439_A7Jgb.seq
>D04886A2_A7Jg_1_BGH_rev2_E03_15_ABI24.ab1 1 CATCGGGTCA GCAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC TCAGCTGCAC 121 TTGCAGGAGC GCACAATCAT GTTGGACAAC TGCTCCACCT TGGGCTTGCG ACCCACGTAG 181 TAGACGATGG GCAGTGGCTC CAAAGCCTGC GGCACGCAGC ACGGTGACGC CGAAGCGCCC 241 GGGTTGTGTT GGTTGTAGAG GGCAAGGACC TTGCTGTACT GTGTGTCCAG GCTCCAAATA 301 TAGGGGCAGG GTCCCAGACA GAAGTTGGCA TGGTAGCCCT TGGGCTCGTG GATCCACTTC 361 CAACCCAGGT CCTTCCTAAA GTCAATGTAC AGCTGCCGCA CACAGCAGTT CTTCTCTGTG 421 GAGCTGAAGC AATAGTTGGT ATCCAGGGCT CTCCGGTGCC GTGAGCTGTG CAGGTGCTGG 481 GCCCTTTCCA GGGGGGTGGC CATGAGGAGC AGGAAGGGCC GGTTCATGTC ATGGATGGTG 541 CCCAGGTCGC CCCGACGTTT GGGGCTGATC CCGTTGATTT CCACGTGGAG TTTGTTATCT 601 TTGCTGTCAC TAGAGCAGTG AGCGCTGAAT CGAAAGCCCT GTATTCCGTC TCCTTGGTTC 661 AGCCACTGCC GTACAACTCC AGTGACGTCA AAAGACAGCC ACTCAGGCGT ATCAGTGGGG 721 GTCAGCAGCC GGTTACCAAG GTAACGCCAG GAATTGTTGC TATATTTCTG GTAGAGTTCC 781 ACATGTTGCT CCACACTTGA TTTTAATCTC TGCAAGCGCA GCTCTGCACG GGACAGCAAT 841 GGGGGTTCGG GCACTGCTTC CCGAATGTCT GACGTATTGA AGAACATATA TATACTGTGT 901 GAGATGTCTT TGGTTTTCTC ATAGATGGCG TTGTTGCGGT CCACCATTAG CACGCGGGTG 961 ACCTCTTTAG CATAGTAGTC CGCTTCGGGC TCCGGCTCTG GGGTCGGCGC TCTCGCCCTG 1021 CCCACCCCGG GTCGCGGGTG CTGTTGTACA AAGCGAGCAC CGCCTCGGGC AGCGGTGGCG 1081 TTACCTCCCT CTGACTTGGG GGGAACTTGG GGCGGGAGCT //
Primer: SV40pro_F Sequence file: RDB15439_A7Jgc.seq
>D04886A2_A7Jg_1_SV40pro_F_F03_18_ABI24.ab1 1 CTTGGTCCTT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT 121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC 661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC 961 CCAACCCTGC CCATCACGAG ATTTCGATTC CCACCCGCCC GCCTTCTATG AAAGGTTGGG 1021 CTTTCGGGAT CGTTTTCCGG GACGCCGGCT GGATGGATTC TTCAGCGCGG GGGATCTCAT 1081 GCTGCAAGTC TTCGTCCAAC CCCCGACCTT GATTAATTTG CCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Ichimura, S., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl