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pcDNA3.1(+)-c.C225S (#RDB15439)

Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225S missense mutation.

Clone info. Expression vector of mouse transforming growth factor-beta 1 (Tgfb1), C225S (c.637T>A) missense mutation. Total RNA was extracted from mouse embryos at embryonic day 18.5.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Tgfb1 cDNA
Depositor|Developer Furuichi, Tatsuya |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する（Ichimura, S et al. Exp. Anim. 66 (2) : 137-144, 2017）。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15439 pcDNA3.1(+)-c.C225S DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(+)-c.C225S was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15439).

Reference section:

Ichimura, S., Sasaki, S., Murata, T., Fukumura, R., Gondo, Y., Ikegawa, S., Furuichi, T., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15439_A7Jgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB15439_A7Jga.seq check
>D04886A2_A7Jg_1_CMV-Forward_D03_12_ABI24.ab1
    1 CGTTCCTCCG GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG
  181 GATCCACTAG TCCAGTGTGG TGGAATTCAT CCCACCTTTG CCGAGGGTTC CCGCTCTCCG
  241 AAGTGCCGTG GGGCGCCGCC TCCCCCATGC CGCCCTCGGG GCTGCGGCTA CTGCCGCTTC
  301 TGCTCCCACT CCCGTGGCTT CTAGTGCTGA CGCCCGGGAG GCCAGCCGCG GGACTCTCCA
  361 CCTGCAAGAC CATCGACATG GAGCTGGTGA AACGGAAGCG CATCGAAGCC ATCCGTGGCC
  421 AGATCCTGTC CAAACTAAGG CTCGCCAGTC CCCCAAGCCA GGGGGAGGTA CCGCCCGGCC
  481 CGCTGCCCGA GGCGGTGCTC GCTTTGTACA ACAGCACCCG CGACCGGGTG GCAGGCGAGA
  541 GCGCCGACCC AGAGCCGGAG CCCGAAGCGG ACTACTATGC TAAAGAGGTC ACCCGCGTGC
  601 TAATGGTGGA CCGCAACAAC GCCATCTATG AGAAAACCAA AGACATCTCA CACAGTATAT
  661 ATATGTTCTT CAATACGTCA GACATTCGGG AAGCAGTGCC CGAACCCCCA TTGCTGTCCC
  721 GTGCAGAGCT GCGCTTGCAG AGATTAAAAT CAAGTGTGGA GCAACATGTG GAACTCTACC
  781 AGAAATATAG CAACAATTCC TGGCGTTACC TTGGTAACCG GCTGCTGACC CCCACTGATA
  841 CGCCTGAGTG GCTGTCTTTT GACGTCACTG GAGTTGTACG GCAGTGGCTG AACCAAGGAG
  901 ACGGAATACA GGGCTTTCGA TTCAGCGCTC ACTGCTCTAG TGACAGCAAA GATAACAAAC
  961 TCCACGTGGT AATCAACGGG ATCAGCCCCC AAACGTCGGG GCGACCCTGC GCACCATCCC
 1021 ATGACATGAA CCGGGCCCTT TCCTGCTTCA TCATTGACAA CCCCCCTGCA AAGGACCAGC
1081 ACTTGCACAG CTCACGGCAC CGGGAGAAGT TC
//
Primer: BGH_rev2 ()
Sequence file: RDB15439_A7Jgb.seq check
>D04886A2_A7Jg_1_BGH_rev2_E03_15_ABI24.ab1
    1 CATCGGGTCA GCAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC TCAGCTGCAC
  121 TTGCAGGAGC GCACAATCAT GTTGGACAAC TGCTCCACCT TGGGCTTGCG ACCCACGTAG
  181 TAGACGATGG GCAGTGGCTC CAAAGCCTGC GGCACGCAGC ACGGTGACGC CGAAGCGCCC
  241 GGGTTGTGTT GGTTGTAGAG GGCAAGGACC TTGCTGTACT GTGTGTCCAG GCTCCAAATA
  301 TAGGGGCAGG GTCCCAGACA GAAGTTGGCA TGGTAGCCCT TGGGCTCGTG GATCCACTTC
  361 CAACCCAGGT CCTTCCTAAA GTCAATGTAC AGCTGCCGCA CACAGCAGTT CTTCTCTGTG
  421 GAGCTGAAGC AATAGTTGGT ATCCAGGGCT CTCCGGTGCC GTGAGCTGTG CAGGTGCTGG
  481 GCCCTTTCCA GGGGGGTGGC CATGAGGAGC AGGAAGGGCC GGTTCATGTC ATGGATGGTG
  541 CCCAGGTCGC CCCGACGTTT GGGGCTGATC CCGTTGATTT CCACGTGGAG TTTGTTATCT
  601 TTGCTGTCAC TAGAGCAGTG AGCGCTGAAT CGAAAGCCCT GTATTCCGTC TCCTTGGTTC
  661 AGCCACTGCC GTACAACTCC AGTGACGTCA AAAGACAGCC ACTCAGGCGT ATCAGTGGGG
  721 GTCAGCAGCC GGTTACCAAG GTAACGCCAG GAATTGTTGC TATATTTCTG GTAGAGTTCC
  781 ACATGTTGCT CCACACTTGA TTTTAATCTC TGCAAGCGCA GCTCTGCACG GGACAGCAAT
  841 GGGGGTTCGG GCACTGCTTC CCGAATGTCT GACGTATTGA AGAACATATA TATACTGTGT
  901 GAGATGTCTT TGGTTTTCTC ATAGATGGCG TTGTTGCGGT CCACCATTAG CACGCGGGTG
  961 ACCTCTTTAG CATAGTAGTC CGCTTCGGGC TCCGGCTCTG GGGTCGGCGC TCTCGCCCTG
 1021 CCCACCCCGG GTCGCGGGTG CTGTTGTACA AAGCGAGCAC CGCCTCGGGC AGCGGTGGCG
1081 TTACCTCCCT CTGACTTGGG GGGAACTTGG GGCGGGAGCT
//
Primer: SV40pro_F ()
Sequence file: RDB15439_A7Jgc.seq check
>D04886A2_A7Jg_1_SV40pro_F_F03_18_ABI24.ab1
    1 CTTGGTCCTT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC
  961 CCAACCCTGC CCATCACGAG ATTTCGATTC CCACCCGCCC GCCTTCTATG AAAGGTTGGG
 1021 CTTTCGGGAT CGTTTTCCGG GACGCCGGCT GGATGGATTC TTCAGCGCGG GGGATCTCAT
1081 GCTGCAAGTC TTCGTCCAAC CCCCGACCTT GATTAATTTG CCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ichimura, S., An ENU-induced p.C225S missense mutation in the mouse Tgfb1 gene does not cause Camurati-Engelmann disease-like skeletal phenotypes. Exp. Anim. 66 (2): 137-144 (2017). PMID 27928112. [link to RRC of NBRP]