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pMSCV-3xFLAG-mp62-PB1/Zinc (#RDB15407)

Retroviral expression vector of mouse p62 deletion mutant (PB1/zinc, 21-102 a.a/123-167 a.a) tagged with 3xFLAG at N-terminal.

Clone info. Retroviral expression vector of mouse p62 deletion mutant (PB1/zinc, 21-102 a.a/123-167 a.a) tagged with 3xFLAG at N-terminal. See Fig.5e of Watanabe, Y. et al. Nat. Commun., 7: 13508, 2016.
Comment Expression was confirmed by the depositor with immunostaining and western blotting.
Vector backbone pMSCV–Flag(3x) (plasmid)
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name mouse p62 cDNA
Depositor|Developer Shimizu, Shigeomi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15407 pMSCV-3xFLAG-mp62-PB1/Zinc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMSCV-3xFLAG-mp62-PB1/Zinc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15407).

Reference section:

Watanabe, Y., Honda, S., Konishi, A., Arakawa, S., Murohashi, M., Yamaguchi, H., Torii, S., Tanabe, M., Tanaka, S., Warabi, E., Shimizu, S., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15407_A7Ikp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F (Pr0730)
Region: gag3', 3xFLAG, insert5'
Sequence file: RDB15407_A7Ika.seq check
>D04516A7_A7Ik_2_gag-M_F_A02_02_ABI24.ab1
    1 ATGTGGTAGC TTTGTAACCC TAAGCCTCCG CCTCCTCTTC CTCCATCCGC CCCGTCTCTC
   61 CCCCTTGAAC CTCCTCGTTC GACCCCGCCT CGATCCTCCC TTTATCCAGC CCTCACTCCT
  121 TCTCTAGGCG CCGGAATTAG ATCCATGGAT TACAAGGATG ACGACGATAA GAGATCCGAT
  181 TACAAGGATG ACGACGATAA GAGATCCGAT TACAAGGATG ACGACGATAA GAGATCCCCC
  241 GGGCTGCAGG AATTCATGGC GTCGTTCACG GTGAAGGCCT ATCTTCTGGG CAAGGAGGAG
  301 GCGACCCGCG AGATCGGATC TAAGGAGTGC CGGCGGGAAC ATCGCCCACC ATGTGCTCAG
  361 GAGGCACCCC GAAACATGGT GAGATCTTTT CCCAACCCCT TTGGCCACCT CTCTGATAGC
  421 TTCTCTCATA GCCGCTGGCT TCGGAAGCTG AAACATGGAC ACTTTGGCTG GCCTGGCTGG
  481 GAGATGGGCC CACCGGGGAA CTGGAGCCCA CGTCCTCCTC GTGCAGGGGA TGGCCGCCCT
  541 TGCCCTACAG CTGAGTCAGC TTCTGCTCCA CCAGAAGATC CCAATGTCAA TTTCCTGAAG
  601 AATGTGGGGG AGAGTGTGGC AGCTGCCCTC AGCCCTCTAG GCATTGAGGT TGACATTGAT
  661 GTGGAACATG GAGGGAAGAG AAGCCGCCTG ACACCCACTA CCCCAGAAAG TTCCAGCACA
  721 GGCACAGAAG ACAAGAGTAA CACTCAGCCA AGCAGCTGCT CTTCGGAAGT CAGCAAACCT
  781 GACGGGGCTG GGGAGGGCCC TGCTCAGTCT CTGACAGAGC AAATGAAAAA GATAGCCTTG
  841 GAGTCGGTGG GACAGCCAGA GGAACAGATG GAGTCGGGAA ACTGCTCAGA GGAGACGATG
  901 ACTGGACACA TTTGTCTTCA AAAGAAGTGG ACCCATCTAC AGGTGAACTC CAGTCTCTAC
  961 AGATGCCAGA ATCGGGAAGG GCCAAGCTCT CTAGACCCCT CACAGGAAGG ACCCACAGGG
 1021 CTGATGAAGC TGCCCTATAC CACATCTCCA CAGAGCTGAT TCCCCGGCCT GATGGAGTCC
1081 TTCTCCTGAT GCTGTCCATG CATTCTCGGA ATGAGGCCGC CTATCTCACC AGGACT
//
Primer: PGK_N_rev (Pr0243)
Region: insert3', 3xFLAG
Sequence file: RDB15407_A7Ikb.seq check
>D04516A7_A7Ik_2_PGK_N_rev_B02_05_ABI24.ab1
    1 CCTGGGCGCT CCCTACCCGG TAGATTCGTT AACCTCGAGT CACAATGGTG GAGGGTGCTT
   61 CGAATACTGG ATCGTGTCCA GAGCAGCCCC GATGTCGTAA TTCTTGGTCT GTAGGAGCCT
  121 GGTGAGCCAG CCGCCTTCAT CCGAGAAACC CATGGACAGC ATCTGGGAGA GGGACTCAAT
  181 CAGCCGGGGA TCAGCCTCTG GTGGGAGATG TGGGTATAGG GCAGCTTCCT TCAGCCCTGT
  241 GGGTCCTTCC TGTGAGGGGT CTAGAGAGCT TGGCCCTTCC GATTCTGGCA TCTGTAGAGA
  301 CTGGAGTTCA CCTGTAGATG GGTCCACTTC TTTTGAAGAC AAATGTGTCC AGTCATCGTC
  361 TCCTCCTGAG CAGTTTCCCG ACTCCATCTG TTCCTCTGGC TGTCCCACCG ACTCCAAGGC
  421 TATCTTTTTC ATTTGCTCTG TCAGAGACTG AGCAGGGCCC TCCCCAGCCC CGTCAGGTTT
  481 GCTGACTTCC GAAGAGCAGC TGCTTGGCTG AGTGTTACTC TTGTCTTCTG TGCCTGTGCT
  541 GGAACTTTCT GGGGTAGTGG GTGTCAGGCG GCTTCTCTTC CCTCCATGTT CCACATCAAT
  601 GTCAACCTCA ATGCCTAGAG GGCTGAGGGC AGCTGCCACA CTCTCCCCCA CATTCTTCAG
  661 GAAATTGACA TTGGGATCTT CTGGTGGAGC AGAAGCTGAC TCAGCTGTAG GGCAAGGGCG
  721 GCCATCCCCT GCACGAGGAG GACGTGGGCT CCAGTTCCCC GGTGGGCCCA TCTCCCAGCC
  781 AGGCCAGCCA AAGTGTCCAT GTTTCAGCTT CCGAAGCCAG CGGCTATGAG AGAAGCTATC
  841 AGAGAGGTGG CCAAAGGGGT TGGGAAAAGA TCTCACCATG TTTCGGGGTG CCTCCTGAGC
  901 ACATGGTGGG CGATGTTCCC GCCGGCACTC CTTAGATCCG ATCTCGCGGG TCGCCTCCTC
  961 CTTGCCCAGA AGATAGGCCT TCACCGTGAA CGACGCCATG AATTCCTGCA GCCCGGGGGA
 1021 TCTCTTATCG TCGTCATCCC TTTGTAATCG GGATCTCTTA TCGTCGTCAT TCTGTTAATC
 1081 GGATCTTCTT ATCGTCGTCA ATCAGTAGTC AATGAATCTA AATTTCCGGC GCCTTAAGAG
1141 GAAGGGAA
//
Primer: pBR322_BamHI_R (Pr0065)
Region: 5'LTR, MESV psi
Sequence file: RDB15407_A7Ikc.seq check
>D04516A7_A7Ik_2_pBR322_BamHI_R_C02_08_ABI24.ab1
    1 TTTTTAGACG GATATCAGTG GTCCAGGCTC TAGTTTTGAC TCAACAATAT CACCAGCTGA
   61 AGCCTATAGA GTACGAGCCA TAGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAAGG
  121 GGGGAATGAA AGACCCCACC TGTAGGTTTG GCAAGCTAGC TTAAGTAACG CCATTTTGCA
  181 AGGCATGGAA AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTTAG GAACAGAGAG
  241 ACAGCAGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG
  301 CCAAGAACAG ATGGTCCCCA GATGCGGTCC CGCCCTCAGC AGTTTCTAGA GAACCATCAG
  361 ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA ACTAACCAAT
  421 CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC GAGCTCAATA AAAGAGCCCA
  481 CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATAGACTGCG TCGCCCGGGT ACCCGTATTC
  541 CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT CGCTGATCCT TGGGAGGGTC
  601 TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT TTGGAGGTTC CACCGAGATT
  661 TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG AGGTAAGCTG GCCAGCGGTC
  721 GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG CATCTAATGT TTGCGCCTGC
  781 GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT
  841 TCTGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTTGGGGGC CGTTTTTGTG
  901 GCCCGACCTG AGGAAGGGAG TCGATGTGGA ATCCGACCCC GTCAGGATAT GTGGTTCTGG
  961 TAGGAGACGA GAACCTAAAA CAGTTCCCGC CTCCGTCTGA ATTTTTGCTT TCGGGTTTGG
 1021 AACCCGAAGC CCGCGCGTCT TGTCTGCTGC AGCCGCCTGC AGCATCGATT CTGTGTTGTT
 1081 CTCTGTTCTG ACTGTTGTTT CTGTATTGGT CCTGGAAAAT AACGGATAGA ACTGTTAACC
1141 AACCTTCCCG TTAAGTTTGG AACC
//
Primer: PGKpro_F (Pr0249)
Region: PGK pro, HygR 5'
Sequence file: RDB15407_A7Ikd.seq check
>D04516A7_A7Ik_2_PGKpro_F_E02_14_ABI24.ab1
    1 ATCGGGCGTA CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCAGCCCAAG CTTATGAAAA
   61 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT
  121 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG
  181 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG
  241 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAGT
  301 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC
  361 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG
  421 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC
  481 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC
  541 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC
  601 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA
  661 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG
  721 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG
  781 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC
  841 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT
  901 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA
  961 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGGCCG TCTGGACCGA TGGCTGTGTA
 1021 GAAGTACTCG CCCGAATAGT GGAAAACCGA CGCCCCAGCA CTCGTCCCGA GGCAAGGAAT
 1081 AGATCGATAA AATTAAAGAT TTTATTTAGG TCTCCAGAAT AAAGGGGGGA AATGCAAAGA
1141 ACCCCCCCCA ACT
//
Primer: HygR-C_F (Pr0501)
Region: HygR 3', 3'LTR
Sequence file: RDB15407_A7Ike.seq check
>D04516A7_A7Ik_2_HygR-C_F_H02_08_ABI08.ab1
    1 CGATGCGGGG GATCCAATAC GAGGTCGCCA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG
   61 TATGGAGCAG CAGACGCGCT ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCGCG
  121 GCTCCGGGCG TATATGCTCC GCATTGGTCT TGACCAACTC TATCAGAGCT TGGTTGACGG
  181 CAATTTCGAT GATGCAGCTT GGGCGCAGGG TCGATGCGAC GCAATCGTCC GATCCGGAGC
  241 CGGGACTGTC GGGCGTACAC AAATCGCCCG CAGAAGCGCG GCCGTCTGGA CCGATGGCTG
  301 TGTAGAAGTA CTCGCCGATA GTGGAAACCG ACGCCCCAGC ACTCGTCCGA GGGCAAAGGA
  361 ATAGATCGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC
  421 CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAATAC
  481 ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTTAGGAACA GAGAGACAGC AGAATATGGG
  541 CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT
  601 CCCCAGATGC GGTCCCGCCC TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC
  661 CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC
  721 TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG
  781 CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT
  841 GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGGA GGGTCTCCTC TGAGTGATTG
  901 ACTACCCGTC AGCGGGGGGT CTTTCATGGG TAACAGTTTC CTTGAAGTTG GAGAACAACA
  961 TTCTGAGGGT AGGAGTCGAA ATATTAAGTA ATCCTGACTC AATTTAGCCA CTGTTTTTGG
 1021 ATTCCACATA CTCCCAATAC TCCTGAATTA GTCCAATTAA TGGACAGCGC CAGAAGAGCC
1081 TTGGGGAGAA TTGCTGCAAC TTGGTTTAAT TCCGGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]