Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMSCV-3xFLAG-mp62-PB1/Zinc

Retroviral expression vector of mouse p62 deletion mutant (PB1/zinc, 21-102 a.a/123-167 a.a) tagged with 3xFLAG at N-terminal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15407_A7Ikp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F Region: gag3', 3xFLAG, insert5' Sequence file: RDB15407_A7Ika.seq
>D04516A7_A7Ik_2_gag-M_F_A02_02_ABI24.ab1     1 ATGTGGTAGC TTTGTAACCC TAAGCCTCCG CCTCCTCTTC CTCCATCCGC CCCGTCTCTC    61 CCCCTTGAAC CTCCTCGTTC GACCCCGCCT CGATCCTCCC TTTATCCAGC CCTCACTCCT   121 TCTCTAGGCG CCGGAATTAG ATCCATGGAT TACAAGGATG ACGACGATAA GAGATCCGAT   181 TACAAGGATG ACGACGATAA GAGATCCGAT TACAAGGATG ACGACGATAA GAGATCCCCC   241 GGGCTGCAGG AATTCATGGC GTCGTTCACG GTGAAGGCCT ATCTTCTGGG CAAGGAGGAG   301 GCGACCCGCG AGATCGGATC TAAGGAGTGC CGGCGGGAAC ATCGCCCACC ATGTGCTCAG   361 GAGGCACCCC GAAACATGGT GAGATCTTTT CCCAACCCCT TTGGCCACCT CTCTGATAGC   421 TTCTCTCATA GCCGCTGGCT TCGGAAGCTG AAACATGGAC ACTTTGGCTG GCCTGGCTGG   481 GAGATGGGCC CACCGGGGAA CTGGAGCCCA CGTCCTCCTC GTGCAGGGGA TGGCCGCCCT   541 TGCCCTACAG CTGAGTCAGC TTCTGCTCCA CCAGAAGATC CCAATGTCAA TTTCCTGAAG   601 AATGTGGGGG AGAGTGTGGC AGCTGCCCTC AGCCCTCTAG GCATTGAGGT TGACATTGAT   661 GTGGAACATG GAGGGAAGAG AAGCCGCCTG ACACCCACTA CCCCAGAAAG TTCCAGCACA   721 GGCACAGAAG ACAAGAGTAA CACTCAGCCA AGCAGCTGCT CTTCGGAAGT CAGCAAACCT   781 GACGGGGCTG GGGAGGGCCC TGCTCAGTCT CTGACAGAGC AAATGAAAAA GATAGCCTTG   841 GAGTCGGTGG GACAGCCAGA GGAACAGATG GAGTCGGGAA ACTGCTCAGA GGAGACGATG   901 ACTGGACACA TTTGTCTTCA AAAGAAGTGG ACCCATCTAC AGGTGAACTC CAGTCTCTAC   961 AGATGCCAGA ATCGGGAAGG GCCAAGCTCT CTAGACCCCT CACAGGAAGG ACCCACAGGG  1021 CTGATGAAGC TGCCCTATAC CACATCTCCA CAGAGCTGAT TCCCCGGCCT GATGGAGTCC 1081 TTCTCCTGAT GCTGTCCATG CATTCTCGGA ATGAGGCCGC CTATCTCACC AGGACT //
Primer: PGK_N_rev Region: insert3', 3xFLAG Sequence file: RDB15407_A7Ikb.seq
>D04516A7_A7Ik_2_PGK_N_rev_B02_05_ABI24.ab1     1 CCTGGGCGCT CCCTACCCGG TAGATTCGTT AACCTCGAGT CACAATGGTG GAGGGTGCTT    61 CGAATACTGG ATCGTGTCCA GAGCAGCCCC GATGTCGTAA TTCTTGGTCT GTAGGAGCCT   121 GGTGAGCCAG CCGCCTTCAT CCGAGAAACC CATGGACAGC ATCTGGGAGA GGGACTCAAT   181 CAGCCGGGGA TCAGCCTCTG GTGGGAGATG TGGGTATAGG GCAGCTTCCT TCAGCCCTGT   241 GGGTCCTTCC TGTGAGGGGT CTAGAGAGCT TGGCCCTTCC GATTCTGGCA TCTGTAGAGA   301 CTGGAGTTCA CCTGTAGATG GGTCCACTTC TTTTGAAGAC AAATGTGTCC AGTCATCGTC   361 TCCTCCTGAG CAGTTTCCCG ACTCCATCTG TTCCTCTGGC TGTCCCACCG ACTCCAAGGC   421 TATCTTTTTC ATTTGCTCTG TCAGAGACTG AGCAGGGCCC TCCCCAGCCC CGTCAGGTTT   481 GCTGACTTCC GAAGAGCAGC TGCTTGGCTG AGTGTTACTC TTGTCTTCTG TGCCTGTGCT   541 GGAACTTTCT GGGGTAGTGG GTGTCAGGCG GCTTCTCTTC CCTCCATGTT CCACATCAAT   601 GTCAACCTCA ATGCCTAGAG GGCTGAGGGC AGCTGCCACA CTCTCCCCCA CATTCTTCAG   661 GAAATTGACA TTGGGATCTT CTGGTGGAGC AGAAGCTGAC TCAGCTGTAG GGCAAGGGCG   721 GCCATCCCCT GCACGAGGAG GACGTGGGCT CCAGTTCCCC GGTGGGCCCA TCTCCCAGCC   781 AGGCCAGCCA AAGTGTCCAT GTTTCAGCTT CCGAAGCCAG CGGCTATGAG AGAAGCTATC   841 AGAGAGGTGG CCAAAGGGGT TGGGAAAAGA TCTCACCATG TTTCGGGGTG CCTCCTGAGC   901 ACATGGTGGG CGATGTTCCC GCCGGCACTC CTTAGATCCG ATCTCGCGGG TCGCCTCCTC   961 CTTGCCCAGA AGATAGGCCT TCACCGTGAA CGACGCCATG AATTCCTGCA GCCCGGGGGA  1021 TCTCTTATCG TCGTCATCCC TTTGTAATCG GGATCTCTTA TCGTCGTCAT TCTGTTAATC  1081 GGATCTTCTT ATCGTCGTCA ATCAGTAGTC AATGAATCTA AATTTCCGGC GCCTTAAGAG 1141 GAAGGGAA //
Primer: pBR322_BamHI_R Region: 5'LTR, MESV psi Sequence file: RDB15407_A7Ikc.seq
>D04516A7_A7Ik_2_pBR322_BamHI_R_C02_08_ABI24.ab1     1 TTTTTAGACG GATATCAGTG GTCCAGGCTC TAGTTTTGAC TCAACAATAT CACCAGCTGA    61 AGCCTATAGA GTACGAGCCA TAGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAAGG   121 GGGGAATGAA AGACCCCACC TGTAGGTTTG GCAAGCTAGC TTAAGTAACG CCATTTTGCA   181 AGGCATGGAA AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTTAG GAACAGAGAG   241 ACAGCAGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG   301 CCAAGAACAG ATGGTCCCCA GATGCGGTCC CGCCCTCAGC AGTTTCTAGA GAACCATCAG   361 ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA ACTAACCAAT   421 CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC GAGCTCAATA AAAGAGCCCA   481 CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATAGACTGCG TCGCCCGGGT ACCCGTATTC   541 CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT CGCTGATCCT TGGGAGGGTC   601 TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT TTGGAGGTTC CACCGAGATT   661 TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG AGGTAAGCTG GCCAGCGGTC   721 GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG CATCTAATGT TTGCGCCTGC   781 GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT   841 TCTGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTTGGGGGC CGTTTTTGTG   901 GCCCGACCTG AGGAAGGGAG TCGATGTGGA ATCCGACCCC GTCAGGATAT GTGGTTCTGG   961 TAGGAGACGA GAACCTAAAA CAGTTCCCGC CTCCGTCTGA ATTTTTGCTT TCGGGTTTGG  1021 AACCCGAAGC CCGCGCGTCT TGTCTGCTGC AGCCGCCTGC AGCATCGATT CTGTGTTGTT  1081 CTCTGTTCTG ACTGTTGTTT CTGTATTGGT CCTGGAAAAT AACGGATAGA ACTGTTAACC 1141 AACCTTCCCG TTAAGTTTGG AACC //
Primer: PGKpro_F Region: PGK pro, HygR 5' Sequence file: RDB15407_A7Ikd.seq
>D04516A7_A7Ik_2_PGKpro_F_E02_14_ABI24.ab1     1 ATCGGGCGTA CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCAGCCCAAG CTTATGAAAA    61 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT   121 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG   181 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG   241 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAGT   301 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC   361 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG   421 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC   481 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC   541 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC   601 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA   661 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG   721 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG   781 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC   841 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT   901 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA   961 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGGCCG TCTGGACCGA TGGCTGTGTA  1021 GAAGTACTCG CCCGAATAGT GGAAAACCGA CGCCCCAGCA CTCGTCCCGA GGCAAGGAAT  1081 AGATCGATAA AATTAAAGAT TTTATTTAGG TCTCCAGAAT AAAGGGGGGA AATGCAAAGA 1141 ACCCCCCCCA ACT //
Primer: HygR-C_F Region: HygR 3', 3'LTR Sequence file: RDB15407_A7Ike.seq
>D04516A7_A7Ik_2_HygR-C_F_H02_08_ABI08.ab1     1 CGATGCGGGG GATCCAATAC GAGGTCGCCA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG    61 TATGGAGCAG CAGACGCGCT ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCGCG   121 GCTCCGGGCG TATATGCTCC GCATTGGTCT TGACCAACTC TATCAGAGCT TGGTTGACGG   181 CAATTTCGAT GATGCAGCTT GGGCGCAGGG TCGATGCGAC GCAATCGTCC GATCCGGAGC   241 CGGGACTGTC GGGCGTACAC AAATCGCCCG CAGAAGCGCG GCCGTCTGGA CCGATGGCTG   301 TGTAGAAGTA CTCGCCGATA GTGGAAACCG ACGCCCCAGC ACTCGTCCGA GGGCAAAGGA   361 ATAGATCGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC   421 CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAATAC   481 ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTTAGGAACA GAGAGACAGC AGAATATGGG   541 CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT   601 CCCCAGATGC GGTCCCGCCC TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC   661 CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC   721 TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG   781 CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT   841 GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGGA GGGTCTCCTC TGAGTGATTG   901 ACTACCCGTC AGCGGGGGGT CTTTCATGGG TAACAGTTTC CTTGAAGTTG GAGAACAACA   961 TTCTGAGGGT AGGAGTCGAA ATATTAAGTA ATCCTGACTC AATTTAGCCA CTGTTTTTGG  1021 ATTCCACATA CTCCCAATAC TCCTGAATTA GTCCAATTAA TGGACAGCGC CAGAAGAGCC 1081 TTGGGGAGAA TTGCTGCAAC TTGGTTTAAT TCCGGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl