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pMSCV-3xFLAG-mp62-del PB1 (#RDB15405)

Retroviral expression vector of mouse p62 deletion mutant (PB1 domain, 21-102 a.a) tagged with 3xFLAG at N-terminal.

Clone info. Retroviral expression vector of mouse p62 deletion mutant (PB1 domain, 21-102 a.a) tagged with 3xFLAG at N-terminal. See Fig.5e of Watanabe, Y. et al. Nat. Commun., 7: 13508, 2016.
Comment Expression was confirmed by the depositor with immunostaining and western blotting.
Vector backbone pMSCV–Flag(3x) (plasmid)
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name mouse p62 cDNA
Depositor|Developer Shimizu, Shigeomi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15405 pMSCV-3xFLAG-mp62-del PB1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMSCV-3xFLAG-mp62-del PB1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15405).

Reference section:

Watanabe, Y., Honda, S., Konishi, A., Arakawa, S., Murohashi, M., Yamaguchi, H., Torii, S., Tanabe, M., Tanaka, S., Warabi, E., Shimizu, S., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15405_A7Ikp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F (Pr0730)
Region: gag3', 3xFLAG, insert5'
Sequence file: RDB15405_A7Ika.seq check
>D04516A5_A7Ik_2_gag-M_F_F06_18_ABI24.ab1
    1 AGGTTGGGCA GCCCTTTGTA CACCCTAAGC CTCCGCCTCC TCTTCCTCCA TCCGCCCCGT
   61 CTCTCCCCCT TGAACCTCCT CGTTCGACCC CGCCTCGATC CTCCCTTTAT CCAGCCCTCA
  121 CTCCTTCTCT AGGCGCCGGA ATTAGATCCA TGGATTACAA GGATGACGAC GATAAGAGAT
  181 CCGATTACAA GGATGACGAC GATAAGAGAT CCGATTACAA GGATGACGAC GATAAGAGAT
  241 CCCCCGGGCT GCAGGAATTC ATGGCGTCGT TCACGGTGAA GGCCTATCTT CTGGGCAAGG
  301 AGGAGGCGAC CCGCGAGATC GGATCTAAGG AGTGCCGGCG GGAACATCGC CCACCATGTG
  361 CTCAGGAGGC ACCCCGAAAC ATGGTGCACC CCAATGTGAT CTGTGATGGT TGCAACGGGC
  421 CTGTGGTGGG AACTCGCTAT AAGTGCAGTG TGTGCCCAGA CTACGACCTG TGCAGCGTGT
  481 GCGAGGGGAA GGGCCTGCAC AGGGAACACA GCAAGCTCAT CTTTCCCAAC CCCTTTGGCC
  541 ACCTCTCTGA TAGCTTCTCT CATAGCCGCT GGCTTCGGAA GCTGAAACAT GGACACTTTG
  601 GCTGGCCTGG CTGGGAGATG GGCCCACCGG GGAACTGGAG CCCACGTCCT CCTCGTGCAG
  661 GGGATGGCCG CCCTTGCCCT ACAGCTGAGT CAGCTTCTGC TCCACCAGAA GATCCCAATG
  721 TCAATTTCCT GAAGAATGTG GGGGAGAGTG TGGCAGCTGC CCTCAGCCCT CTAGGCATTG
  781 AGGTTGACAT TGATGTGGAA CATGGAGGGA AGAGAAGCCG CCTGACACCC ACTACCCCAG
  841 AAAGTTCCAG CACAGGCACA GAAGACAAGA GTAACACTCA GCCAAGCAGC TGCTCTTCGG
  901 AAGTCAGCAA ACCTGACGGG GCTGGGGAGG GCCCTGCTCA GTCTCTGACA GAGCAAATGA
  961 AAAAGATAGC CCTTGGAGTC GGTGGGACAG CCAGAGGAAC AGATGGGAGT CGGGAACTGC
 1021 TCAGAGAGAC GATGACTGCA CACATTTGTC TTCAAAGAGT GGACCCAATC TACAGGTGAC
 1081 TCCAGTCTCT ACGATGCAGA TTCGGCACGG ACCAGCCTCC TCTAGACCCT CGTTTCACAG
//
Primer: PGK_N_rev (Pr0243)
Region: PGK pro, insert3'
Sequence file: RDB15405_A7Ikb.seq check
>D04516A5_A7Ik_2_PGK_N_rev_G06_21_ABI24.ab1
    1 CATGTTACGG CCTCCCCTAC CCGGTAGAAT TCGTTAACCT CGAGTCACAA TGGTGGAGGG
   61 TGCTTCGAAT ACTGGATCGT GTCCAGAGCA GCCCCGATGT CGTAATTCTT GGTCTGTAGG
  121 AGCCTGGTGA GCCAGCCGCC TTCATCCGAG AAACCCATGG ACAGCATCTG GGAGAGGGAC
  181 TCAATCAGCC GGGGATCAGC CTCTGGTGGG AGATGTGGGT ATAGGGCAGC TTCCTTCAGC
  241 CCTGTGGGTC CTTCCTGTGA GGGGTCTAGA GAGCTTGGCC CTTCCGATTC TGGCATCTGT
  301 AGAGACTGGA GTTCACCTGT AGATGGGTCC ACTTCTTTTG AAGACAAATG TGTCCAGTCA
  361 TCGTCTCCTC CTGAGCAGTT TCCCGACTCC ATCTGTTCCT CTGGCTGTCC CACCGACTCC
  421 AAGGCTATCT TTTTCATTTG CTCTGTCAGA GACTGAGCAG GGCCCTCCCC AGCCCCGTCA
  481 GGTTTGCTGA CTTCCGAAGA GCAGCTGCTT GGCTGAGTGT TACTCTTGTC TTCTGTGCCT
  541 GTGCTGGAAC TTTCTGGGGT AGTGGGTGTC AGGCGGCTTC TCTTCCCTCC ATGTTCCACA
  601 TCAATGTCAA CCTCAATGCC TAGAGGGCTG AGGGCAGCTG CCACACTCTC CCCCACATTC
  661 TTCAGGAAAT TGACATTGGG ATCTTCTGGT GGAGCAGAAG CTGACTCAGC TGTAGGGCAA
  721 GGGCGGCCAT CCCCTGCACG AGGAGGACGT GGGCTCCAGT TCCCCGGTGG GCCCATCTCC
  781 CAGCCAGGCC AGCCAAAGTG TCCATGTTTC AGCTTCCGAA GCCAGCGGCT ATGAGAGAAG
  841 CTATCAGAGA GGTGGCCAAA GGGGTTGGGA AAGATGAGCT TGCTGTGTTC CCTGTGCAGG
  901 CCCTTCCCCT CGCACACGCT GCACAGGTCG TAGTCTGGGC ACACACTGCA CTTATAGCGA
  961 GTTCCCACCA CAGGCCCGTT GCAACCATCA CAGATCACAT TGGGGGTGCA CCATGTTTCG
 1021 GGTGCCTCCT GAGCACATGG TGGGCGATGT TCCCGCCGGC ACTCCTTTAG ATCCGATCTC
 1081 GCGGGTCGCC TTCCTTCTGC CAGAAGAATA GCACTTCACC GTGAACCGAC GCCTAATTGA
1141 AT
//
Primer: pBR322_BamHI_R (Pr0065)
Region: 5'LTR, MESV psi
Sequence file: RDB15405_A7Ikc.seq check
>D04516A5_A7Ik_2_pBR322_BamHI_R_H06_24_ABI24.ab1
    1 TTTCTAGGAA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG
   61 AAGCCTATAG AGTACGAGCC ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG
  121 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC
  181 AAGGCATGGA AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA
  241 GACAGCAGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG
  301 GCCAAGAACA GATGGTCCCC AGATGCGGTC CCGCCCTCAG CAGTTTCTAG AGAACCATCA
  361 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA
  421 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC
  481 ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATAGACTGC GTCGCCCGGG TACCCGTATT
  541 CCCAATAAAG CCTCTTGCTG TTTGCATCCG AATCGTGGAC TCGCTGATCC TTGGGAGGGT
  601 CTCCTCAGAT TGATTGACTG CCCACCTCGG GGGTCTTTCA TTTGGAGGTT CCACCGAGAT
  661 TTGGAGACCC CTGCCCAGGG ACCACCGACC CCCCCGCCGG GAGGTAAGCT GGCCAGCGGT
  721 CGTTTCGTGT CTGTCTCTGT CTTTGTGCGT GTTTGTGCCG GCATCTAATG TTTGCGCCTG
  781 CGTCTGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG AACTGACGAG
  841 TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG GCCGTTTTTG
  901 TGGCCCGACC TGAGGAAAGG GAGTCGATGT GGAATCCGAC CCCGTCAGGA TATGTGGGTT
  961 CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGGT
 1021 TGGAACCGAA AGCCGCGCGT CTTGTCTGCT GCAGCGCTGC AGCATCGTTC TGTGTGTCTC
 1081 TGTCTGACTG TGTTTCCTGT ATTTGATCTG AATAAGGGCA GACTGATTAC CCACCTCCCT
1141 TAAGGTTG
//
Primer: PGKpro_F (Pr0249)
Region: PGK pro, HygR 5'
Sequence file: RDB15405_A7Ikd.seq check
>D04516A5_A7Ik_2_PGKpro_F_B01_04_ABI24.ab1
    1 AGCGGGGCGT ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGCAGCCCA AGCTTATGAA
   61 AAAGCCTGAA CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT
  121 CTCCGACCTG ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG
  181 AGGGCGTGGA TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA
  241 TGTTTATCGG CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA
  301 GTTCAGCGAG AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA
  361 CCTGCCTGAA ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT
  421 CGCTGCGGCC GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG
  481 TCAATACACT ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG
  541 GCAAACTGTG ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT
  601 GCTTTGGGCC GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA
  661 CAATGTCCTG ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT
  721 CGGGGATTCC CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT
  781 GGAGCAGCAG ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT
  841 CCGGGCGTAT ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTGACGGCAA
  901 TTTCGATGAT GCAGCTTGGG GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGGAGCC
  961 GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG CCCGTCTGGA CCGATGGGCT
 1021 GTGTAGAAAG TACTCGCCCG ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGGAGGCACG
 1081 ATAGATCGAT AAAATTAAAG ATTTTATTTA GGTCTCAGAA AAGGGGGAAT GAAAAAGAAC
1141 CCCC
//
Primer: HygR-C_F (Pr0501)
Region: HygR 3', 3'LTR
Sequence file: RDB15405_A7Ike.seq check
>D04516A5_A7Ik_2_HygR-C_F_F02_06_ABI08.ab1
    1 CGGTGCCGGG GGATCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT
   61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG
  121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC
  181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA
  241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC
  301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG
  361 GAATAGATCG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA
  421 CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAT
  481 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA GCAGAATATG
  541 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG
  601 GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT
  661 GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC
  721 GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC
  781 GGCGCGCCAG TCCTCCGATA GACTGCGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC
  841 TTGCAGTTTG CATCCGACTT GTGGTCTCGC TGTTTCCTTG GGAGGGTCTC CTCTGAGTGA
  901 TTGACTACCC GTCAGCGGGG GGTCTTTCAT GGGTAACAGT TTCTTGAAGT TGGAGAACCA
  961 ACATTCTGAG GGTAGGAGTC GAAATATTTA AGTAAATCCT GACTCAAATT AGCCCACTGA
 1021 TTTGATCCAC ATACTCCAAT ACCTCCATGA AATAGGTTTC ATTATGGGAC CAGCCGCCAG
1081 AGAGCCTGGG GGAGAACTTG TTGAAACTTG GTTATTCCGC T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]