Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMSCV-3xFLAG-mp62-del PB1

Retroviral expression vector of mouse p62 deletion mutant (PB1 domain, 21-102 a.a) tagged with 3xFLAG at N-terminal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15405_A7Ikp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F Region: gag3', 3xFLAG, insert5' Sequence file: RDB15405_A7Ika.seq
>D04516A5_A7Ik_2_gag-M_F_F06_18_ABI24.ab1     1 AGGTTGGGCA GCCCTTTGTA CACCCTAAGC CTCCGCCTCC TCTTCCTCCA TCCGCCCCGT    61 CTCTCCCCCT TGAACCTCCT CGTTCGACCC CGCCTCGATC CTCCCTTTAT CCAGCCCTCA   121 CTCCTTCTCT AGGCGCCGGA ATTAGATCCA TGGATTACAA GGATGACGAC GATAAGAGAT   181 CCGATTACAA GGATGACGAC GATAAGAGAT CCGATTACAA GGATGACGAC GATAAGAGAT   241 CCCCCGGGCT GCAGGAATTC ATGGCGTCGT TCACGGTGAA GGCCTATCTT CTGGGCAAGG   301 AGGAGGCGAC CCGCGAGATC GGATCTAAGG AGTGCCGGCG GGAACATCGC CCACCATGTG   361 CTCAGGAGGC ACCCCGAAAC ATGGTGCACC CCAATGTGAT CTGTGATGGT TGCAACGGGC   421 CTGTGGTGGG AACTCGCTAT AAGTGCAGTG TGTGCCCAGA CTACGACCTG TGCAGCGTGT   481 GCGAGGGGAA GGGCCTGCAC AGGGAACACA GCAAGCTCAT CTTTCCCAAC CCCTTTGGCC   541 ACCTCTCTGA TAGCTTCTCT CATAGCCGCT GGCTTCGGAA GCTGAAACAT GGACACTTTG   601 GCTGGCCTGG CTGGGAGATG GGCCCACCGG GGAACTGGAG CCCACGTCCT CCTCGTGCAG   661 GGGATGGCCG CCCTTGCCCT ACAGCTGAGT CAGCTTCTGC TCCACCAGAA GATCCCAATG   721 TCAATTTCCT GAAGAATGTG GGGGAGAGTG TGGCAGCTGC CCTCAGCCCT CTAGGCATTG   781 AGGTTGACAT TGATGTGGAA CATGGAGGGA AGAGAAGCCG CCTGACACCC ACTACCCCAG   841 AAAGTTCCAG CACAGGCACA GAAGACAAGA GTAACACTCA GCCAAGCAGC TGCTCTTCGG   901 AAGTCAGCAA ACCTGACGGG GCTGGGGAGG GCCCTGCTCA GTCTCTGACA GAGCAAATGA   961 AAAAGATAGC CCTTGGAGTC GGTGGGACAG CCAGAGGAAC AGATGGGAGT CGGGAACTGC  1021 TCAGAGAGAC GATGACTGCA CACATTTGTC TTCAAAGAGT GGACCCAATC TACAGGTGAC  1081 TCCAGTCTCT ACGATGCAGA TTCGGCACGG ACCAGCCTCC TCTAGACCCT CGTTTCACAG //
Primer: PGK_N_rev Region: PGK pro, insert3' Sequence file: RDB15405_A7Ikb.seq
>D04516A5_A7Ik_2_PGK_N_rev_G06_21_ABI24.ab1     1 CATGTTACGG CCTCCCCTAC CCGGTAGAAT TCGTTAACCT CGAGTCACAA TGGTGGAGGG    61 TGCTTCGAAT ACTGGATCGT GTCCAGAGCA GCCCCGATGT CGTAATTCTT GGTCTGTAGG   121 AGCCTGGTGA GCCAGCCGCC TTCATCCGAG AAACCCATGG ACAGCATCTG GGAGAGGGAC   181 TCAATCAGCC GGGGATCAGC CTCTGGTGGG AGATGTGGGT ATAGGGCAGC TTCCTTCAGC   241 CCTGTGGGTC CTTCCTGTGA GGGGTCTAGA GAGCTTGGCC CTTCCGATTC TGGCATCTGT   301 AGAGACTGGA GTTCACCTGT AGATGGGTCC ACTTCTTTTG AAGACAAATG TGTCCAGTCA   361 TCGTCTCCTC CTGAGCAGTT TCCCGACTCC ATCTGTTCCT CTGGCTGTCC CACCGACTCC   421 AAGGCTATCT TTTTCATTTG CTCTGTCAGA GACTGAGCAG GGCCCTCCCC AGCCCCGTCA   481 GGTTTGCTGA CTTCCGAAGA GCAGCTGCTT GGCTGAGTGT TACTCTTGTC TTCTGTGCCT   541 GTGCTGGAAC TTTCTGGGGT AGTGGGTGTC AGGCGGCTTC TCTTCCCTCC ATGTTCCACA   601 TCAATGTCAA CCTCAATGCC TAGAGGGCTG AGGGCAGCTG CCACACTCTC CCCCACATTC   661 TTCAGGAAAT TGACATTGGG ATCTTCTGGT GGAGCAGAAG CTGACTCAGC TGTAGGGCAA   721 GGGCGGCCAT CCCCTGCACG AGGAGGACGT GGGCTCCAGT TCCCCGGTGG GCCCATCTCC   781 CAGCCAGGCC AGCCAAAGTG TCCATGTTTC AGCTTCCGAA GCCAGCGGCT ATGAGAGAAG   841 CTATCAGAGA GGTGGCCAAA GGGGTTGGGA AAGATGAGCT TGCTGTGTTC CCTGTGCAGG   901 CCCTTCCCCT CGCACACGCT GCACAGGTCG TAGTCTGGGC ACACACTGCA CTTATAGCGA   961 GTTCCCACCA CAGGCCCGTT GCAACCATCA CAGATCACAT TGGGGGTGCA CCATGTTTCG  1021 GGTGCCTCCT GAGCACATGG TGGGCGATGT TCCCGCCGGC ACTCCTTTAG ATCCGATCTC  1081 GCGGGTCGCC TTCCTTCTGC CAGAAGAATA GCACTTCACC GTGAACCGAC GCCTAATTGA 1141 AT //
Primer: pBR322_BamHI_R Region: 5'LTR, MESV psi Sequence file: RDB15405_A7Ikc.seq
>D04516A5_A7Ik_2_pBR322_BamHI_R_H06_24_ABI24.ab1     1 TTTCTAGGAA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG    61 AAGCCTATAG AGTACGAGCC ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG   121 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC   181 AAGGCATGGA AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA   241 GACAGCAGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG   301 GCCAAGAACA GATGGTCCCC AGATGCGGTC CCGCCCTCAG CAGTTTCTAG AGAACCATCA   361 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA   421 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC   481 ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATAGACTGC GTCGCCCGGG TACCCGTATT   541 CCCAATAAAG CCTCTTGCTG TTTGCATCCG AATCGTGGAC TCGCTGATCC TTGGGAGGGT   601 CTCCTCAGAT TGATTGACTG CCCACCTCGG GGGTCTTTCA TTTGGAGGTT CCACCGAGAT   661 TTGGAGACCC CTGCCCAGGG ACCACCGACC CCCCCGCCGG GAGGTAAGCT GGCCAGCGGT   721 CGTTTCGTGT CTGTCTCTGT CTTTGTGCGT GTTTGTGCCG GCATCTAATG TTTGCGCCTG   781 CGTCTGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG AACTGACGAG   841 TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG GCCGTTTTTG   901 TGGCCCGACC TGAGGAAAGG GAGTCGATGT GGAATCCGAC CCCGTCAGGA TATGTGGGTT   961 CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGGT  1021 TGGAACCGAA AGCCGCGCGT CTTGTCTGCT GCAGCGCTGC AGCATCGTTC TGTGTGTCTC  1081 TGTCTGACTG TGTTTCCTGT ATTTGATCTG AATAAGGGCA GACTGATTAC CCACCTCCCT 1141 TAAGGTTG //
Primer: PGKpro_F Region: PGK pro, HygR 5' Sequence file: RDB15405_A7Ikd.seq
>D04516A5_A7Ik_2_PGKpro_F_B01_04_ABI24.ab1     1 AGCGGGGCGT ATCTCTCTTC TCATCTCCGG GCCTTTCGAC CTGCAGCCCA AGCTTATGAA    61 AAAGCCTGAA CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT   121 CTCCGACCTG ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG   181 AGGGCGTGGA TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA   241 TGTTTATCGG CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA   301 GTTCAGCGAG AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA   361 CCTGCCTGAA ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT   421 CGCTGCGGCC GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG   481 TCAATACACT ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG   541 GCAAACTGTG ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT   601 GCTTTGGGCC GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA   661 CAATGTCCTG ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT   721 CGGGGATTCC CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT   781 GGAGCAGCAG ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT   841 CCGGGCGTAT ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTGACGGCAA   901 TTTCGATGAT GCAGCTTGGG GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGGAGCC   961 GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG CCCGTCTGGA CCGATGGGCT  1021 GTGTAGAAAG TACTCGCCCG ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGGAGGCACG  1081 ATAGATCGAT AAAATTAAAG ATTTTATTTA GGTCTCAGAA AAGGGGGAAT GAAAAAGAAC 1141 CCCC //
Primer: HygR-C_F Region: HygR 3', 3'LTR Sequence file: RDB15405_A7Ike.seq
>D04516A5_A7Ik_2_HygR-C_F_F02_06_ABI08.ab1     1 CGGTGCCGGG GGATCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT    61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG   121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC   181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA   241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC   301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG   361 GAATAGATCG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA   421 CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAT   481 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA GCAGAATATG   541 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG   601 GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT   661 GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC   721 GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC   781 GGCGCGCCAG TCCTCCGATA GACTGCGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC   841 TTGCAGTTTG CATCCGACTT GTGGTCTCGC TGTTTCCTTG GGAGGGTCTC CTCTGAGTGA   901 TTGACTACCC GTCAGCGGGG GGTCTTTCAT GGGTAACAGT TTCTTGAAGT TGGAGAACCA   961 ACATTCTGAG GGTAGGAGTC GAAATATTTA AGTAAATCCT GACTCAAATT AGCCCACTGA  1021 TTTGATCCAC ATACTCCAAT ACCTCCATGA AATAGGTTTC ATTATGGGAC CAGCCGCCAG 1081 AGAGCCTGGG GGAGAACTTG TTGAAACTTG GTTATTCCGC T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl