Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMSCV-3xFLAG-hCep63

Retroviral expression vector of human Cep63 tagged with 3xFLAG at N-terminal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15404_A7Ikp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F Region: gag3', 3xFLAG, insert5' Sequence file: RDB15404_A7Ika.seq
>D04516A4_A7Ik_1_gag-M_F_A06_03_ABI24.ab1     1 ATTTGGCAGC CTTTGTACAC CCTAAGCCTC CGCCTCCTCT TCCTCCATCC GCCCCGTCTC    61 TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA GCCCTCACTC   121 CTTCTCTAGG CGCCGGAATT AGATCCATGG ATTACAAGGA TGACGACGAT AAGAGATCCG   181 ATTACAAGGA TGACGACGAT AAGAGATCCG ATTACAAGGA TGACGACGAT AAGAGATCCA   241 TGGAGGCTTT GTTAGAAGGA ATACAAAATC GAGGGCATGG TGGGGGATTT TTGACATCTT   301 GTGAAGCAGA ACTACAGGAG CTCATGAAAC AGATTGACAT AATGGTGGCT CATAAAAAAT   361 CTGAATGGGA AGGACGTACA CATGCTCTAG AAACTTGCTT GAAAATCCGT GAACAGGAAC   421 TTAAGAGTCT TAGGAGTCAG TTGGATGTGA CACATAAGGA GGTTGGAATG TTGCATCAGC   481 AGGTAGAAGA ACATGAAAAA ATCAAGCAAG AGATGACCAT GGAATATAAG CAGGAGTTGA   541 AGAAACTACA TGAAGAATTA TGCATACTGA AGAGAAGCTA TGAAAAGCTT CAGAAAAAGC   601 AAATGAGGGA ATTCAGAGGA AATACCAAAA ATCACAGGGA AGATCGGTCT GAAATTGAGA   661 GGTTAACTGC AAAAATAGAG GAATTCCGTC AGAAATCGCT GGACTGGGAG AAGCAACGCT   721 TGATTTATCA GCAACAGGTA TCTTCACTGG AGGCACAAAG GAAGGCTCTG GCTGAACAAT   781 CAGAGATAAT TCAGGCTCAG CTTGTCAATC GGAAACAGAA ATTAGAGTCT GTGGAACTTT   841 CTAGCCAATC AGAAATTCAA CACTTAAGCA GTAAACTGGA GCGGGCTAAT GACACTATCT   901 GTGCCAATGA GTTGGAAATA GAGCGCCTCA CCATGAGGGT CAATGACTTG GTTTGGAACC   961 AGTATGACTG TCCTACAGGG AGCAGCAGCA AAAAGAAGAA AAATTTGAGG ATCTGAAAAA  1021 CTATTAGAGG GCTCTGCAGA GAAAAGAGAG AATTTGAAGC CAGCTCCTTC CAGTCTCAGA  1081 TATCTCCATA CATGGAGTCA GGATTACAAA AGAGAGGTAC CAGGAAGTAA GCCTACTTAC 1141 ATCTTCAGA //
Primer: PGK_N_rev Region: insert3' Sequence file: RDB15404_A7Ikb.seq
>D04516A4_A7Ik_1_PGK_N_rev_B06_06_ABI24.ab1     1 CCTGGTGCGG CCTCCCTACC CGGTAGATTC GTTAACCTCG AGCTACTTTA AGGCTGTGAA    61 TTGTCTCACT GTCTTTTCAC TCTCTCTTTT TAGTTCTTCA ATATGGGCAT CCAAGCGCTC   121 TAGAATGTGA TGAGACCTCA GTTCCTCCTC TTCCAAAAAT CTGGTAGCTA TTGAACCAAG   181 GGGAGATACA GGCAAGGAAC ACGAAGATAT AAACTCTTCT TGGTCATTCA TACTCTCAGA   241 CATAGTGTCA GAAAAATTGG CTTCATTTGT ATCTAGCGCT GAAGGCAAAG AATGAGTTTT   301 ACATAGAGAG TAGGAAGTCA AAGACCTGGT GGAGCTGCAA GGGCTGATTT GAGGACTCAG   361 GGGGCTTAGC ACCCTAGACA TGGGGTTTAT ACTATCCGAC GCTTGTCCTC TTGGAGAATG   421 AAGATCTGTT TTGTAGTGCT CAGTCTTTAT TCCATCATGT CTGTGCTGGC CATGGGTTGG   481 CTTGAACTCT GTATTCTTGA ACTCAGTTGT TCTGCTGTGT GTTGGTTTAA AGATCCTGTC   541 ATTTTGTTTT TTGCACATCT GAGTAGAAAT CTCCAGCTGA GATTTGGTAT TCATCAAATC   601 TTTCTGTAGT TGTTGATTTT CAGACACTAA TTTATTTAAT GCCTCAATAT AATTCTCCTG   661 GAGGTCTGCT AGTGAAATCT CTTTTGCCTC ATGTAAACCC AATTCCAATT TCATCACCAT   721 TTCAGAAAGA TGACGATTTT CTAATTTGAG TGACTCCAGC TGATCCAAAA TCTCCTTATG   781 CTCTACTGCT TTGTCTTCTG CCTTTTGCAT CTCTGCTCTG AGTCGCTTTT CCATGTCTGA   841 GGAAGAACCT TTGGTGGAAG CAATAGTGAT ATCTCGCTGA TGTAACTCCT GAGTTAGATG   901 GGAGATTTCC ATCTTCATTC CTTCTAGTGC AGAACTGTAA CTTTGTTCAC CCTGTAAAAT   961 CTGTTCTTTC AACTTCTTAA TCTCTGCTTT GTATTCATTG TTATGTGCTT CCATCCCTCT  1021 TTCAGTTTCT TTCATATTTT TCCATTTAGT TCTTGGCTCA GCTGTTTACA CGTTGCACTC  1081 ACAGATTCCC AAACTGCCTC CAGACAGGTT CAACGTCTGC CCTGCAGAAG GTCCCTCAAC 1141 TTAGTAATTG //
Primer: pBR322_BamHI_R Region: 5'LTR, MESV psi Sequence file: RDB15404_A7Ikc.seq
>D04516A4_A7Ik_1_pBR322_BamHI_R_C06_09_ABI24.ab1     1 TGTGGATAGG AAGGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG    61 CTGAAGCCTA TAGAGTACGA GCCATAGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA   121 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT   181 TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG   241 AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC   301 AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA   361 TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC   421 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG   481 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT   541 ATTCCCAATA AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG   601 GGTCTCCTCA GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA   661 GATTTGGAGA CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC   721 GGTCGTTTCG TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC   781 CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC   841 GAGTTCTGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGGCCGTTT   901 TTGTGGCCCG ACCTGAGGAA GGGAGTCGAT GTGGAAATCC GACCCCGTCA GGATATGTGG   961 TTCTGGTAGG AGACGAGAAC CTAAACAGTT TCCCGCCTCC GTCTGAATTT TTGCTTTTCG  1021 CTTGGAACCC GAAGCCGCGC GTCTGTCTGC CTGCAGCCGC TGCAGCATCG TTCCTGTGGT  1081 GGTCTCTTGT CTGACTGTGT TTCTGTATTG TCTGGAAATT AGGGCCCAGA CTGGTTACCC 1141 ACTTCCTCTT ATAAGTTGA //
Primer: PGKpro_F Region: PGK pro, HygR 5' Sequence file: RDB15404_A7Ikd.seq
>D04516A4_A7Ik_1_PGKpro_F_B07_04_ABI24.ab1     1 ATTGGCGTAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCAGCCCAAG CTTATGAAAA    61 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT   121 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG   181 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG   241 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAGT   301 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC   361 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG   421 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC   481 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC   541 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC   601 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA   661 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG   721 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG   781 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC   841 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT   901 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA   961 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGGCCG TCTGGACCCG ATGGCTGTGT  1021 AGAAAGTACT CGCCGATAGT GGAAACCCGA CGCCCCAGCA CTCGTCCGAG GGCAAAGGTA 1081 TAGATTCGGA TAAATTAAAA GAATTTTTAA TTTTAAGTTC TTTCCAAGAA AAAAAA //
Primer: HygR-C_F Region: HygR 3', 3'LTR Sequence file: RDB15404_A7Ike.seq
>D04516A4_A7Ik_1_HygR-C_F_E02_05_ABI08.ab1     1 CGAATCGGGG GGATCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT    61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG   121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC   181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA   241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC   301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG   361 GAATAGATCG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA   421 CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAT   481 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA GCAGAATATG   541 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG   601 GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT   661 GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC   721 GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC   781 GGCGCGCCAG TCCTCCGATA GACTGCGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC   841 TTGCAGTTGC ATCCGACTTG TGGTCTCGCT GTTCCTTGGG AGGGTCTCCT CTGAGTGATT   901 GACTACCCGT CAGCGGGGGG TCTTTCATGG GTAACAGTTT CTTGAAGTTG GGAGAACAAC   961 ATTCTGAGGG TAGGAGTCGG AATATTAAAG TAATCCTGAC TCATTTAGCC ACTGGTTTTG  1021 AATCCACATA CTCCCAATAC TTCCTTGAAT TAGGTTCCAT TATGGACAGC GCAGAGAAGC 1081 CTGGGGGGAG GAATTGCTGC AAAACTTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl