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pMSCV-3xFLAG-hCep63 (#RDB15404)

Retroviral expression vector of human Cep63 tagged with 3xFLAG at N-terminal.

Clone info. Retroviral expression vector of human Cep63 tagged with 3xFLAG at N-terminal.
Comment Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pMSCV–Flag(3x) (plasmid)
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name human CEP63 cDNA
Depositor|Developer Shimizu, Shigeomi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15404 pMSCV-3xFLAG-hCep63 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMSCV-3xFLAG-hCep63 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15404).

Reference section:

Watanabe, Y., Honda, S., Konishi, A., Arakawa, S., Murohashi, M., Yamaguchi, H., Torii, S., Tanabe, M., Tanaka, S., Warabi, E., Shimizu, S., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15404_A7Ikp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F (Pr0730)
Region: gag3', 3xFLAG, insert5'
Sequence file: RDB15404_A7Ika.seq check
>D04516A4_A7Ik_1_gag-M_F_A06_03_ABI24.ab1
    1 ATTTGGCAGC CTTTGTACAC CCTAAGCCTC CGCCTCCTCT TCCTCCATCC GCCCCGTCTC
   61 TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA GCCCTCACTC
  121 CTTCTCTAGG CGCCGGAATT AGATCCATGG ATTACAAGGA TGACGACGAT AAGAGATCCG
  181 ATTACAAGGA TGACGACGAT AAGAGATCCG ATTACAAGGA TGACGACGAT AAGAGATCCA
  241 TGGAGGCTTT GTTAGAAGGA ATACAAAATC GAGGGCATGG TGGGGGATTT TTGACATCTT
  301 GTGAAGCAGA ACTACAGGAG CTCATGAAAC AGATTGACAT AATGGTGGCT CATAAAAAAT
  361 CTGAATGGGA AGGACGTACA CATGCTCTAG AAACTTGCTT GAAAATCCGT GAACAGGAAC
  421 TTAAGAGTCT TAGGAGTCAG TTGGATGTGA CACATAAGGA GGTTGGAATG TTGCATCAGC
  481 AGGTAGAAGA ACATGAAAAA ATCAAGCAAG AGATGACCAT GGAATATAAG CAGGAGTTGA
  541 AGAAACTACA TGAAGAATTA TGCATACTGA AGAGAAGCTA TGAAAAGCTT CAGAAAAAGC
  601 AAATGAGGGA ATTCAGAGGA AATACCAAAA ATCACAGGGA AGATCGGTCT GAAATTGAGA
  661 GGTTAACTGC AAAAATAGAG GAATTCCGTC AGAAATCGCT GGACTGGGAG AAGCAACGCT
  721 TGATTTATCA GCAACAGGTA TCTTCACTGG AGGCACAAAG GAAGGCTCTG GCTGAACAAT
  781 CAGAGATAAT TCAGGCTCAG CTTGTCAATC GGAAACAGAA ATTAGAGTCT GTGGAACTTT
  841 CTAGCCAATC AGAAATTCAA CACTTAAGCA GTAAACTGGA GCGGGCTAAT GACACTATCT
  901 GTGCCAATGA GTTGGAAATA GAGCGCCTCA CCATGAGGGT CAATGACTTG GTTTGGAACC
  961 AGTATGACTG TCCTACAGGG AGCAGCAGCA AAAAGAAGAA AAATTTGAGG ATCTGAAAAA
 1021 CTATTAGAGG GCTCTGCAGA GAAAAGAGAG AATTTGAAGC CAGCTCCTTC CAGTCTCAGA
 1081 TATCTCCATA CATGGAGTCA GGATTACAAA AGAGAGGTAC CAGGAAGTAA GCCTACTTAC
1141 ATCTTCAGA
//
Primer: PGK_N_rev (Pr0243)
Region: insert3'
Sequence file: RDB15404_A7Ikb.seq check
>D04516A4_A7Ik_1_PGK_N_rev_B06_06_ABI24.ab1
    1 CCTGGTGCGG CCTCCCTACC CGGTAGATTC GTTAACCTCG AGCTACTTTA AGGCTGTGAA
   61 TTGTCTCACT GTCTTTTCAC TCTCTCTTTT TAGTTCTTCA ATATGGGCAT CCAAGCGCTC
  121 TAGAATGTGA TGAGACCTCA GTTCCTCCTC TTCCAAAAAT CTGGTAGCTA TTGAACCAAG
  181 GGGAGATACA GGCAAGGAAC ACGAAGATAT AAACTCTTCT TGGTCATTCA TACTCTCAGA
  241 CATAGTGTCA GAAAAATTGG CTTCATTTGT ATCTAGCGCT GAAGGCAAAG AATGAGTTTT
  301 ACATAGAGAG TAGGAAGTCA AAGACCTGGT GGAGCTGCAA GGGCTGATTT GAGGACTCAG
  361 GGGGCTTAGC ACCCTAGACA TGGGGTTTAT ACTATCCGAC GCTTGTCCTC TTGGAGAATG
  421 AAGATCTGTT TTGTAGTGCT CAGTCTTTAT TCCATCATGT CTGTGCTGGC CATGGGTTGG
  481 CTTGAACTCT GTATTCTTGA ACTCAGTTGT TCTGCTGTGT GTTGGTTTAA AGATCCTGTC
  541 ATTTTGTTTT TTGCACATCT GAGTAGAAAT CTCCAGCTGA GATTTGGTAT TCATCAAATC
  601 TTTCTGTAGT TGTTGATTTT CAGACACTAA TTTATTTAAT GCCTCAATAT AATTCTCCTG
  661 GAGGTCTGCT AGTGAAATCT CTTTTGCCTC ATGTAAACCC AATTCCAATT TCATCACCAT
  721 TTCAGAAAGA TGACGATTTT CTAATTTGAG TGACTCCAGC TGATCCAAAA TCTCCTTATG
  781 CTCTACTGCT TTGTCTTCTG CCTTTTGCAT CTCTGCTCTG AGTCGCTTTT CCATGTCTGA
  841 GGAAGAACCT TTGGTGGAAG CAATAGTGAT ATCTCGCTGA TGTAACTCCT GAGTTAGATG
  901 GGAGATTTCC ATCTTCATTC CTTCTAGTGC AGAACTGTAA CTTTGTTCAC CCTGTAAAAT
  961 CTGTTCTTTC AACTTCTTAA TCTCTGCTTT GTATTCATTG TTATGTGCTT CCATCCCTCT
 1021 TTCAGTTTCT TTCATATTTT TCCATTTAGT TCTTGGCTCA GCTGTTTACA CGTTGCACTC
 1081 ACAGATTCCC AAACTGCCTC CAGACAGGTT CAACGTCTGC CCTGCAGAAG GTCCCTCAAC
1141 TTAGTAATTG
//
Primer: pBR322_BamHI_R (Pr0065)
Region: 5'LTR, MESV psi
Sequence file: RDB15404_A7Ikc.seq check
>D04516A4_A7Ik_1_pBR322_BamHI_R_C06_09_ABI24.ab1
    1 TGTGGATAGG AAGGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG
   61 CTGAAGCCTA TAGAGTACGA GCCATAGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA
  121 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  181 TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG
  241 AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
  301 AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA
  361 TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC
  421 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG
  481 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT
  541 ATTCCCAATA AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG
  601 GGTCTCCTCA GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA
  661 GATTTGGAGA CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC
  721 GGTCGTTTCG TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC
  781 CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC
  841 GAGTTCTGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGGCCGTTT
  901 TTGTGGCCCG ACCTGAGGAA GGGAGTCGAT GTGGAAATCC GACCCCGTCA GGATATGTGG
  961 TTCTGGTAGG AGACGAGAAC CTAAACAGTT TCCCGCCTCC GTCTGAATTT TTGCTTTTCG
 1021 CTTGGAACCC GAAGCCGCGC GTCTGTCTGC CTGCAGCCGC TGCAGCATCG TTCCTGTGGT
 1081 GGTCTCTTGT CTGACTGTGT TTCTGTATTG TCTGGAAATT AGGGCCCAGA CTGGTTACCC
1141 ACTTCCTCTT ATAAGTTGA
//
Primer: PGKpro_F (Pr0249)
Region: PGK pro, HygR 5'
Sequence file: RDB15404_A7Ikd.seq check
>D04516A4_A7Ik_1_PGKpro_F_B07_04_ABI24.ab1
    1 ATTGGCGTAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCAGCCCAAG CTTATGAAAA
   61 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT
  121 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG
  181 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG
  241 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAGT
  301 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC
  361 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG
  421 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC
  481 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC
  541 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC
  601 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA
  661 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG
  721 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG
  781 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC
  841 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT
  901 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA
  961 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGGCCG TCTGGACCCG ATGGCTGTGT
 1021 AGAAAGTACT CGCCGATAGT GGAAACCCGA CGCCCCAGCA CTCGTCCGAG GGCAAAGGTA
1081 TAGATTCGGA TAAATTAAAA GAATTTTTAA TTTTAAGTTC TTTCCAAGAA AAAAAA
//
Primer: HygR-C_F (Pr0501)
Region: HygR 3', 3'LTR
Sequence file: RDB15404_A7Ike.seq check
>D04516A4_A7Ik_1_HygR-C_F_E02_05_ABI08.ab1
    1 CGAATCGGGG GGATCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT
   61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG
  121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC
  181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA
  241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC
  301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG
  361 GAATAGATCG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA
  421 CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAT
  481 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA GCAGAATATG
  541 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG
  601 GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT
  661 GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC
  721 GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC
  781 GGCGCGCCAG TCCTCCGATA GACTGCGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC
  841 TTGCAGTTGC ATCCGACTTG TGGTCTCGCT GTTCCTTGGG AGGGTCTCCT CTGAGTGATT
  901 GACTACCCGT CAGCGGGGGG TCTTTCATGG GTAACAGTTT CTTGAAGTTG GGAGAACAAC
  961 ATTCTGAGGG TAGGAGTCGG AATATTAAAG TAATCCTGAC TCATTTAGCC ACTGGTTTTG
 1021 AATCCACATA CTCCCAATAC TTCCTTGAAT TAGGTTCCAT TATGGACAGC GCAGAGAAGC
1081 CTGGGGGGAG GAATTGCTGC AAAACTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]