RDB15396 - pEF-T7-mouse GARI-L4

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pEF-T7-mouse GARI-L4 (#RDB15396)

Expression vector of mouse GARI-L4, N-terminal T7-tag.

Clone info.	Expression vector of mouse Golgi-associated Rab2B interactor-like 4 (GARI-L4) [Fam71a in NCBI], tagged with N-terminal T7, EF1 promoter.
Comment	Expression was confirmed by the depositor with immunoblotting.
Vector backbone	pEF-T7 (plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	mouse Fam71a cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. and Fukuda, M., J. Biol. Chem. 290 (36): 22250-22261, 2015)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. and Fukuda, M., J. Biol. Chem. 290 (36): 22250-22261, 2015) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aizawa, M. and Fukuda, M., J. Biol. Chem. 290 (36): 22250-22261, 2015)。 MTAに書く付加的使用条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aizawa, M. and Fukuda, M., J. Biol. Chem. 290 (36): 22250-22261, 2015)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15396	pEF-T7-mouse GARI-L4	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-mouse GARI-L4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15396).

Reference section:

Aizawa, M., Fukuda, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15396_A7Ibp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert(T7 tag,GARI-L4 5') Sequence file: RDB15396_A7Iba.seq
>D04916C5_A7Ib_2_EF1a-C_F_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTTNGGNN CTTGGTTNNT TCTCAAGCCT CAGACAGTGG 61 TTCAAAGTTT TTTTCTTCCN TTTCAGGTGT CGTGAGGAAT TCTCTAGAGC GGCCGCGGGA 121 ATTCGATTCC ACCATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGCG GATCCATGGA 181 CGTAAAGGAA GAGTCTCTGT TACCATATTA TACAGCCCAG AGTGGTGCTG AAGTCGGCAT 241 GTTCAACGGC TTGTTCGACA CCGCCACGGG GAAATTACAG CAGCAGTTGC ACAAAGGAGA 301 GTATGATATA TTCAAGTACG CGCCGATATT CGAGAGCGAC TTCATCCAGA TCACCAGATA 361 TGGAGAAGTG ATCGACGTGC ACAATCGTGT CCGAATGGTG ACTGTGGGCA TTGCGTGCAC 421 CAGTCCCATC CTTCCACTCC CTGATGTCAT GCTGCTGGCC CGACCAGCAC CATTCGATGA 481 GCTTCTGGGA CATGGCCAGT CCACCAACAG CACCAAAGGC AAAAAACGCA AGGCTGGAAA 541 GACCTTGGAG CTCACTAGGC TCCTTCCTTT GAAGTTTGTA AGGATTTCTG TTCACAGACG 601 TGATGAACAA CAGCTACGTC TCAAGTTTGC TACTGGCCGT TCTTGCTACC TGCAGTTGTG 661 CCCCCCTCCC GAAAGCAAGG ATGATCTCTT TTCCTACTGG GAAAAATTGA TTGACCTCCT 721 GAGACCACCT GTGGACAGTA ACACCAGTAC CTATGCCATC CCAGCANGGG ACATGATGAG 781 CACGCCTTTT CTGGATGAAG AACTGAAGAT ACCAAAAAAA AAANGCAAAA GAAAATCCGG 841 CCACCNAAAA AAAAGANATC TATACAANGT CAGCGNCAAA AGCCTCCCCN CGANCACAGA 901 TGTNNNNGCG ATCAGCNCTT CNGCTTTTTT CNNGNGGGGN NGNAAANCCN NNANNNTCAN 961 ATNNNNNNNA NNNNNNNNNN //
Primer: hGCSF_R (Pr0031) Region: insert(GARI-L4 3') Sequence file: RDB15396_A7Ibb.seq
>D04916C5_A7Ib_2_hGCSF_R_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNGGGGNNCC TCACTCTAGN NCGGCCGCGA 61 ATTCACTAGT GATTTTAATG AGCTTCAAAG GTCACTGTCT CCATGACCTC GGATGTCACA 121 GAATCAATGA TTTCCAGGCC CTGGCCATCC TCTGCCTCCA TGATGGCCTT TACGTCGGTT 181 GTCTCTAATG TCTTAGTCAT GATGCCCACA TCTTGGCCAC GTGGTGCCAG GCTTTTTCCA 241 GCCAAGCTGG CTCTGATGTT TCTCAGGAAC AAGCTGATTC TGCCCACCCT CTTGCTCGTA 301 GAGGCAGAGC TGGCAGTGGA GATGGTCCGG GACAGTTTGT GAGAGCTCCG GGACTCCTTT 361 GTGATGGGGA CATGGCTGAC CCCTTTGTGC CACCTATTGC GCTTGCTGCC TTTGTCCTTG 421 TCATCGCCAA CACCTCTGTG ACTGGTTGAT TTTTGAAACT CCTTGTCCCC TTCTGTCTTG 481 TGACCTTCTC CTGTCCTCTG GTGCTGCTCA CTCCTGCTGC CAACTCTTTC CTTTGCTCTC 541 TGGTCCCTTC TCGACCTCCT TTCCTTATGG GCTTTGGTAC TGGTCTCATA GGCTTTCCGA 601 CGTCCAGTCT CTTCATTTGA GTGGCTTTCG CTTGGCAAGG TCAAGGCGAG GACAACTGGG 661 ACTGGGTCAT TTGCTGTTTC TCCAACAGAC TTTCCTGTAN TCTCAATCCT GGAATTTGTT 721 ACAGTCATGA TCTCTTTGCG GGANANGCTG GCAGGTGAAC TGGGAATATA CTCCAAGGAC 781 TTGCTTGCCA TCCCTGCCAA GGNAATCTTC ATGCTCTTTG GCGACATTTT GCCAGTGCTT 841 GCTATGGTCA AATGGCTTTT GCTTCCTCTT GCTCCCNANG GCANCCTCCT CTACTGNANC 901 TTTNTTTATC TGNCCCNAGC CTTCNNNNTG NTNGCTGCCN ANCTCAAAGA NTCTACNNNN 961 NTCACACNCT ANTNNCNNNT GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634. [link to RRC of NBRP]

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Other resources in our bank

mouse Fam71a

External Database
mouse Fam71a

Reference sequence