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pMRX-puro-HA-mouse PAT4 (#RDB15392)

Retroviral expression vector of mouse PAT, N-terminal HA-tag.

Clone info. Retroviral expression vector of mouse proton assisted amino acid transporter 4 (PAT4) [Slc36a4 in NCBI], tagged with N-terminal HA, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-IRES-puro (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Slc36a4 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsui, T. and Fukuda, M., EMBO Rep. 14 (5): 450-457, 2013)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Matsui, T. and Fukuda, M., EMBO Rep. 14 (5): 450-457, 2013)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15392 pMRX-puro-HA-mouse PAT4 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMRX-puro-HA-mouse PAT4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15392).

Reference section:

Matsui, T., Fukuda, M., Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4. EMBO Rep. 14 (5): 450-457 (2013). PMID 23478338. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15392_A7Ibp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol,insert 5'(HA,mPAT4 5')
Sequence file: RDB15392_A7Iba.seq check
>15392_15392_B2C2F_MMLV-pol_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNGGNATCG CAGCTTGGAN ACNCGCCGCC CACGTGAAGG CTGCCGACCC
   61 CGGGGGTGGA CCATCCTCTA GACTGCCGGA TCTCCACCAT GGGTTACCCA TACGACGTCC
  121 CAGACTACGC TGGATCCATG GAAGCGCCGG CGCCGGCGGA GACGGCGGGA TGCGAGGAGC
  181 TCGATATGGA CGTGATGAGG CCCTTAATAA ACGAGCAGAA TTTCGATGGG TCGTCCGACG
  241 AGGAGCAGGA ACAGACGCTT GTGCCCATAC AGAAGCACTA CCAGCTCGAT GGGCAGCACG
  301 GGATTTCGTT CCTGCAGACC TTGGTGCATC TTCTCAAGGG GAACATCGGG ACTGGCCTTT
  361 TAGGGCTCCC CTTGGCTATA AAGAACGCGG GCATCGTGCT TGGACCAATC AGCCTTGTGT
  421 TTATAGGAAT TATTTCCGTC CACTGTATGC ACATATTGGT ACGTTGCAGT CACTTTCTAT
  481 GTCAGAGGTT TAAGAAGTCA ACATTGGGGT ACAGTGACAC CGTGAGTTTT GCCATGGAGG
  541 CTAGTCCGTG GAGTTGCCTT CNGCGACAGG CAGCATGGGG GCGGANCGTG GTCNACTTCN
  601 TTCTGGTGAT AACNCNGCTG GGNNTCNGCA GCGTCTACNT TGNCNTCNNA NCTGANANTG
661 NGNAANNNNN NNNTNNNNNN NNNN
//
Primer: IRES-F (Pr0036)
Region: IRES ,PuroR 5'
Sequence file: RDB15392_A7Ibb.seq check
>D04916C1_A7Ib_1_IRES-F_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNTNNGT CGAGGTTNNN AAACGTCTAG GCCCCCCGAA CCNCGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
  121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC
  181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT
  241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
  301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
  361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
  421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
  481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
  541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
  601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
  661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
  721 GCAGCGCCCG ANCGAAAGGA GCGCACGANC CCATGGCTCG ACGATAAAAT AAAAGATTTT
  781 ATTTAGTCTC CAGAAAAAGG NNGGAATGAA AGANCCCACC TGTANGTTTG GNAAGCTAGC
  841 TTAAGTNACG CCNTTTTGCN AGGNATGGAA AAATACNTAA CTGANAATAG ANAANNNCNN
  901 ATCAAGGTCN NNAACAGATG GNNCAGCTGN ANATGGGNCA AACNNNANAT CTGNGGTAAG
  961 CAGTTNCNGN CNNNGNTCNN NNNNANANNA NANGGNNNAG CTNNNNTGGN NNNANNNNNN
1021 NNNN
//
Primer: IRES-R (Pr0037)
Region: insert 3'(mPAT4 3')
Sequence file: RDB15392_A7Ibc.seq check
>15392_15392_B2C2F_IRES-R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNANCG TTAGGGGGGG GGGCGGAATT TACGTAGCGG CCGCGAATTC
   61 ACTAGTGATT TTGATCATTA CAAACCACTT GTTATGCATG TAGAGTTCAC ATTCAGAAAG
  121 GGACTCTGGG AGGTGCCAGC AACAACTGCC GTAGTCGGAT AAATAATTTC TTCAACAGTG
  181 ACATAGGTGC CCAACAAGAA GCCCACGACT CCAGTGAACG CTATGGAAAT GTTTTTCAGG
  241 ATCATCCATA TGTTGTAGTG GTCCTTAGAA AATGTAAGGA TTTCCACAAG TGGGGGCAGG
  301 ATCAGGGCTA GTGTACTGCT GCTCACAGCC CCCACGAAGG AGATCACAAT GTCTAGGCGA
  361 GGAATGAGAA TCGCCCCAGC ACAGGTGATA CTAACCAAGA GGGACCGTAT CCCAAATTCA
  421 CAAATGCGCT TCCATTTGGC ATGAAGTCTA GCAGTGACTC CAGGGATAAT GATCTCTGCT
  481 GGGACATAGA ACTGAATTGA ATAGGTCACA AAAATGCCAA AGGAATACAG AATTTTCACT
  541 GACTGATACA ACCACATGTC CTGGGGAAGA TTCAGTGTAA TGCTGCCTTT GATCTCGTCN
  601 CGGAAACACA TGTAGCCTAA AGTGGCTAGG CTGANGTACA GCACGGNGAC GATGGNNNTG
  661 CCNATGTTCN NNGNCTGNNG NNNNGTTTGG NNTCTCTNNT TTGGTTTTCC NNNNNNANCN
721 NNNNNNNNN
//
Primer: MMLV psi_F (Pr0863)
Region: MMVV psi,gag,pol
Sequence file: RDB15392_A7Ibd.seq check
>15392_15392_B2C2F_MMLVpsi_F_C01_03_ABI08.ab1
    1 NNNNNNNNNC NNNNNNNNNN NTTTNCTTTC GGTTTGGGAC CGAAGCCGCG CCGCGCGTCT
   61 TGTCTGCTGC AGCATCGTTC TGTGTTGTCT CTGTCTGACT GTGTTTCTGT ATTTGTCTGA
  121 AAATATGGGC CCGGGCCAGA CTGTTACCAC TCCCTTAAGT TTGACCTTAG GTCACTGGAA
  181 AGATGTCGAG CGGATCGCTC ACAACCAGTC GGTAGATGTC AAGAAGAGAC GTTGGGTTAC
  241 CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG CCGCGAGACG GCACCTTTAA
  301 CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA CCTGGCCCGC ATGGACACCC
  361 AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT TTTGACCCCC CTCCCTGGGT
  421 CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCCT CCATCCGCCC CGTCTCTCCC
  481 CCTTGAACCT CCTCGTTCGA CCCCGCCTCG ATCCTCCCTT TATCCAGCCC TCACTCCTTC
  541 TCTAGGCGCC CCCATATGGC CNTATGAGAT CTTATATGGG GCACCCCCGC CCCTTGNAAA
  601 CTTCCCTGAN CCTGACNTGA CAAGAGTTAC TAACAGCCCC TCTCTNCAAG CTNNCTTACA
  661 GGCTCTCTAC TTANTCCNGC ACGAAGTCNG GNNNNNTCNG NNNGCNNCCT ANNNNNNANN
721 NCNNNNNNNA NNNNNNNNNC NNNNNNNNNN
//
Primer: PuroR_F (Pr0554)
Region: PuroR 3',3' LTR
Sequence file: RDB15392_A7Ibe.seq check
>15392_15392_B2C2F_PuroR_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNCNNNGTGC CCGAAGGANC GCGCACCTGG TGCATGACCC GNNNGCCCGG
   61 TGCCTGACGC CCGCCCCACG ACCCGCAGCG CCCGACCGAA AGGAGCGCAC GACCCCATGG
  121 CTCGACGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA AAGGGGGGAA TGAAAGACCC
  181 CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC
  241 ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG
  301 GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG
  361 AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG
  421 GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG CAGTTTCTAG AGAACCATCA
  481 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA
  541 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC
  601 ACAACCCCTC ACTCGGGGCG CCNGTCCTCC GATTGACTGA GTCGCCCGGG TANCCGNGTA
  661 TCCNATAAAC CCTCNNGCAN TTGCNTCCNA NTNGNGGNCT CGCTNNNCNN NNNNNNGNNC
721 NNNNNNANNG ANNGNNNNNC NNNNNNNNGG GGNNNNNNNN NN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,CMV en,deltta U3 5'LTR
Sequence file: RDB15392_A7Ibf.seq check
>D04916C1_A7Ib_1_Amp_R_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGANC ATTNATCNGG GTTATTGTCT CNTGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
  181 GGCCCTTTCG TCTTCACTCG AGGAGCTTGG CCCATTGCAT ACGTTGTATC CATATCATAA
  241 TATGTACATT TATATTGGCT CATGTCCAAC ATTACCGCCA TGTTGACATT GATTATTGAC
  301 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
  361 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
  421 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
  481 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
  541 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
  601 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
  661 CATGCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GANCTGAAAT GANCCTGTGC
  721 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
  781 AGCTCAATAA AANAGCCCAC AACCCCTCAC TCGGCGCGCC NGTCCTCCGA TTGANTGANT
  841 CGCCCGGGTA CCCGNGTATN CNATAAACCC TCTTGNAGTT GCNTCCGACT TGTGNTCTCG
  901 CTGNTCCTNG GGANGGTCTC CTCTGANNGN NTGANNANCN NNCAGCGGGG GNCTTNNNTT
  961 TGGGGNNTCG NCCNGNNNNN GNNNANCNNN NNCCAGGGNN NANNNANCNN NNNNNNGNNN
1021 NTNNNNNNGN NNNNNNNNTN NNNNGNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsui, T., Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4. EMBO Rep. 14 (5): 450-457 (2013). PMID 23478338. [link to RRC of NBRP]