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pEF-Myc-mouse EHD1

Expression vector of mouse EHD1, N-terminal Myc-tag.

Catalog number RDB15386
Resource name pEF-Myc-mouse EHD1
Clone info. Expression vector of mouse EH-domain containing 1 (EHD1), tagged with N-terminal Myc, EF1 promoter.
Expression was confirmed by the depositor with immunoprecipitation and microscopy.
Vector backbone pEF-Myc (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Ehd1 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Ehd1 (NCBI Gene 13660) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15386 pEF-Myc-mouse EHD1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Kobayashi, H., Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth. J Cell Sci. 2013 Jun 1;126(Pt 11):2424-2435 (2013). PMID 23572513.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15386_A7Ibp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15386_A7Iba.seq
>D04916B4_A7Ib_1_EF1a-C_F_F07_16_ABI24.ab1
    1 TCGATCATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCTCTAGAA
  121 CTAGTGGATC TGCCACCATG GAGCAAAAGC TCATTTCTGA AGAGGACTTG AATGAAGGAT
  181 CCATGTTCAG CTGGGTGAGC AAGGATGCCC GCCGCAAGAA GGAGCCGGAG CTCTTCCAGA
  241 CGGTGGCCGA GGGGCTGCGG CAGCTGTACG CGCAGAAGCT GCTGCCGCTG GAGGAGCACT
  301 ATCGCTTCCA CGAGTTCCAC TCGCCCGCGC TGGAGGACGC TGACTTCGAC AACAAGCCGA
  361 TGGTGCTCCT GGTCGGCCAG TACAGCACCG GCAAGACCAC CTTCATCCGC CACCTGATCG
  421 AGCAGGACTT CCCGGGGATG CGCATCGGGC CGGAGCCCAC CACCGACTCC TTCATCGCGG
  481 TCATGCACGG CCCCACCGAG GGCGTGGTGC CCGGCAACGC GCTCGTCGTG GACCCGCGGC
  541 GCCCCTTCCG CAAGCTCAAC GCCTTCGGCA ACGCCTTCCT CAACAGGTTC ATGTGTGCAC
  601 AGCTGCCCAA CCCAGTACTG GACAGCATCA GCATCATTGA CACTCCTGGG ATCCTGTCTG
  661 GGGAGAAGCA GCGCATCAGC CGAGGTTATG ACTTTGCGGC TGTCCTTGAG TGGTTCGCAG
  721 AGCGTGTGGA CCGCATCATC TTGTTGTTCG ACGCCCACAA GCTGGACATC TCAGACGAGT
  781 TCTCAGAAGT CATCAAGGCC CTCAAAAATC ACGAGGACAA GATCCGTGTG GTGCTGAACA
  841 AGGCTGATCA GATCGAGACG CAGCAGCTGA TGCGAGTATA CGGGGCCCTC ATGTGGTCCC
  901 TGGGGAAGAT CATCAACACC CCCGAGGTGG TCAGAGTCTA CATCGGCTCC TTCTGGGTCA
  961 CACCCACTGC TCATCCCTGA CACCCGGAAA GCTCTTTCGA GGCAGAGGAG CAGGACCTCT
 1021 TCAAAGACAT CCAGTCTCTG CCGAGAAACG TCGCCCTCAG AGCTCATGAC CTCATCCAGC
 1081 GGTCAGCTGG CCAAGGTCAT GCCTACATCA TCAGCTTCCC TCAGAGGAGA ATGCCCAAAT
1141 GATTTTCC
//
Primer: hGCSF_R RDB15386_A7Ibb.seq
>D04916B4_A7Ib_1_hGCSF_R_G07_19_ABI24.ab1
    1 GGGGGACTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCGGGAAT
   61 TCGATTTAGT CGACTCACTC GTGCCTCCGT TTGGAGGGTG GAATGAGATG TGGAGGAAGG
  121 TCAGCGGGCA GCTCGTGGCC CTCTAGCTTC ACCTTGATAA GGTGGTTGGC CAGGGCAAAC
  181 TCCTCGTCAT CCAGCAGGCC ATCCTTGTCC ACATCTGCCA ACTTCCAGAT CTTCCCCAGC
  241 ACTGTGTTGG GCAGCTTGGA CTTCACCATC TCCTTCTTGG CATTAGCACC TGTGATCTTG
  301 CCGTTGACAG GAGACAGTGT GTAGAAGATC TCATCATAGG TGGGCTTGTC CTTGCCAACT
  361 ACCCACTCAA CATCATCAAT GCCCTCGCCA GCCCCCTCGC CGTAGCCATG CCCAAAGGGC
  421 CCATTCATGG TGCCATCAAA AGCACCACCC TTCACAGCCT GTGAGGGCAT CAGGGACTCC
  481 TCCTGGCGCA CCATCACCAT CAGCCGAGCT ATATCGTTGG CCAGCATATC ATCCACTGTA
  541 TCCAGCAGCT TGGGCTTCAA GGCCTGGAAC TTGCTGAAGT CCTGGGTCTG CAGGAGTTCC
  601 TGCATCTTAC GCAGGCTTGG GAAGTCGCCG GAGGAGATCT GGTGCTCCCG CTCGATCTTC
  661 TGGTAGATCT CTCCCAGGTT GTTCACCAGC TCTTTCTTCT TGCTCTCTTT CCCGAAAACA
  721 TTGGGCATCT CCTTCTTGAG GGAGCTGATG ATGTAGGCAT GGACCTTGGC CAGCCTGGCC
  781 CGCTTGATGA GGTCATTGAG CTTCCTGAGG GCGGCGTTTC TCGGCAGAGA CTGGATGTCT
  841 TTGAAGAGGT CCTGCTCCTC TGCCTCGAAG AGCTTCCGGT TGTCAGGGAT GAGCAGTGGG
  901 TGTGACCAGA AGGAGCCGAT GTAGACTCTG ACCACCTCGG GGGTGTTGAT GATCTTCCCC
  961 AGGGACCACA TGAGGGCCCC GTATACTCGC ATCAGCTGCT GCGTCTCGAT CTGATCAGCC
 1021 CTTGTTCAGC ACCACACGGA TCTTGTCCTC GTGATTTTTG AGGGCCTTGA TGACTTTCTG
 1081 AGAACTCGTC TGAGATGTCC AGCTTGTTGG GGGCGTTCGA ACCACCAGGA TGGATGCCGG
1141 TCCACACACG CCTCTTGGCG AAACCAACCT TCCAAGGGAA CAAGAGTCGG GCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kobayashi, H., Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth. J Cell Sci. 2013 Jun 1;126(Pt 11):2424-2435 (2013). PMID 23572513.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl