Sequencing and PCR primers for primer information.
Expression vector of mouse EHD1, N-terminal Myc-tag.
| Catalog number | RDB15386 |
|---|---|
| Resource name | pEF-Myc-mouse EHD1 |
| Clone info. | Expression vector of mouse EH-domain containing 1 (EHD1), tagged with N-terminal Myc, EF1 promoter. Expression was confirmed by the depositor with immunoprecipitation and microscopy. |
| Vector backbone | pEF-Myc (plasmid) |
| Size of vector backbone | 5.7 kb |
| Selectable markers | Amp^r |
| Gene/insert name | mouse Ehd1 cDNA |
| Depositor | Fukuda, Mitsunori | |
| Other clones in our bank | mouse Ehd1 (NCBI Gene 13660) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013) |
|---|---|
| Additional terms and conditions for distribution | Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15386 | pEF-Myc-mouse EHD1 | DNA solution |
Electronic file
Featured content
References
| original | Kobayashi, H., Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth. J Cell Sci. 2013 Jun 1;126(Pt 11):2424-2435 (2013). PMID 23572513. |
|---|
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15386_A7Ibp1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: EF1a-C_F | RDB15386_A7Iba.seq |
|---|---|
|
>D04916B4_A7Ib_1_EF1a-C_F_F07_16_ABI24.ab1 1 TCGATCATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC 61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCTCTAGAA 121 CTAGTGGATC TGCCACCATG GAGCAAAAGC TCATTTCTGA AGAGGACTTG AATGAAGGAT 181 CCATGTTCAG CTGGGTGAGC AAGGATGCCC GCCGCAAGAA GGAGCCGGAG CTCTTCCAGA 241 CGGTGGCCGA GGGGCTGCGG CAGCTGTACG CGCAGAAGCT GCTGCCGCTG GAGGAGCACT 301 ATCGCTTCCA CGAGTTCCAC TCGCCCGCGC TGGAGGACGC TGACTTCGAC AACAAGCCGA 361 TGGTGCTCCT GGTCGGCCAG TACAGCACCG GCAAGACCAC CTTCATCCGC CACCTGATCG 421 AGCAGGACTT CCCGGGGATG CGCATCGGGC CGGAGCCCAC CACCGACTCC TTCATCGCGG 481 TCATGCACGG CCCCACCGAG GGCGTGGTGC CCGGCAACGC GCTCGTCGTG GACCCGCGGC 541 GCCCCTTCCG CAAGCTCAAC GCCTTCGGCA ACGCCTTCCT CAACAGGTTC ATGTGTGCAC 601 AGCTGCCCAA CCCAGTACTG GACAGCATCA GCATCATTGA CACTCCTGGG ATCCTGTCTG 661 GGGAGAAGCA GCGCATCAGC CGAGGTTATG ACTTTGCGGC TGTCCTTGAG TGGTTCGCAG 721 AGCGTGTGGA CCGCATCATC TTGTTGTTCG ACGCCCACAA GCTGGACATC TCAGACGAGT 781 TCTCAGAAGT CATCAAGGCC CTCAAAAATC ACGAGGACAA GATCCGTGTG GTGCTGAACA 841 AGGCTGATCA GATCGAGACG CAGCAGCTGA TGCGAGTATA CGGGGCCCTC ATGTGGTCCC 901 TGGGGAAGAT CATCAACACC CCCGAGGTGG TCAGAGTCTA CATCGGCTCC TTCTGGGTCA 961 CACCCACTGC TCATCCCTGA CACCCGGAAA GCTCTTTCGA GGCAGAGGAG CAGGACCTCT 1021 TCAAAGACAT CCAGTCTCTG CCGAGAAACG TCGCCCTCAG AGCTCATGAC CTCATCCAGC 1081 GGTCAGCTGG CCAAGGTCAT GCCTACATCA TCAGCTTCCC TCAGAGGAGA ATGCCCAAAT 1141 GATTTTCC // |
|
| Primer: hGCSF_R | RDB15386_A7Ibb.seq |
|
>D04916B4_A7Ib_1_hGCSF_R_G07_19_ABI24.ab1 1 GGGGGACTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCGGGAAT 61 TCGATTTAGT CGACTCACTC GTGCCTCCGT TTGGAGGGTG GAATGAGATG TGGAGGAAGG 121 TCAGCGGGCA GCTCGTGGCC CTCTAGCTTC ACCTTGATAA GGTGGTTGGC CAGGGCAAAC 181 TCCTCGTCAT CCAGCAGGCC ATCCTTGTCC ACATCTGCCA ACTTCCAGAT CTTCCCCAGC 241 ACTGTGTTGG GCAGCTTGGA CTTCACCATC TCCTTCTTGG CATTAGCACC TGTGATCTTG 301 CCGTTGACAG GAGACAGTGT GTAGAAGATC TCATCATAGG TGGGCTTGTC CTTGCCAACT 361 ACCCACTCAA CATCATCAAT GCCCTCGCCA GCCCCCTCGC CGTAGCCATG CCCAAAGGGC 421 CCATTCATGG TGCCATCAAA AGCACCACCC TTCACAGCCT GTGAGGGCAT CAGGGACTCC 481 TCCTGGCGCA CCATCACCAT CAGCCGAGCT ATATCGTTGG CCAGCATATC ATCCACTGTA 541 TCCAGCAGCT TGGGCTTCAA GGCCTGGAAC TTGCTGAAGT CCTGGGTCTG CAGGAGTTCC 601 TGCATCTTAC GCAGGCTTGG GAAGTCGCCG GAGGAGATCT GGTGCTCCCG CTCGATCTTC 661 TGGTAGATCT CTCCCAGGTT GTTCACCAGC TCTTTCTTCT TGCTCTCTTT CCCGAAAACA 721 TTGGGCATCT CCTTCTTGAG GGAGCTGATG ATGTAGGCAT GGACCTTGGC CAGCCTGGCC 781 CGCTTGATGA GGTCATTGAG CTTCCTGAGG GCGGCGTTTC TCGGCAGAGA CTGGATGTCT 841 TTGAAGAGGT CCTGCTCCTC TGCCTCGAAG AGCTTCCGGT TGTCAGGGAT GAGCAGTGGG 901 TGTGACCAGA AGGAGCCGAT GTAGACTCTG ACCACCTCGG GGGTGTTGAT GATCTTCCCC 961 AGGGACCACA TGAGGGCCCC GTATACTCGC ATCAGCTGCT GCGTCTCGAT CTGATCAGCC 1021 CTTGTTCAGC ACCACACGGA TCTTGTCCTC GTGATTTTTG AGGGCCTTGA TGACTTTCTG 1081 AGAACTCGTC TGAGATGTCC AGCTTGTTGG GGGCGTTCGA ACCACCAGGA TGGATGCCGG 1141 TCCACACACG CCTCTTGGCG AAACCAACCT TCCAAGGGAA CAAGAGTCGG GCA // |
|
Please visit Sequencing and PCR primers for primer information.
References
| original | Kobayashi, H., Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth. J Cell Sci. 2013 Jun 1;126(Pt 11):2424-2435 (2013). PMID 23572513. |
|---|
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl