Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-Myc-mouse EHD1

Expression vector of mouse EHD1, N-terminal Myc-tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013)
Additional terms and conditions for distribution	Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013)。
付加的提供条件	pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15386_A7Ibp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Sequence file: RDB15386_A7Iba.seq
>D04916B4_A7Ib_1_EF1a-C_F_F07_16_ABI24.ab1     1 TCGATCATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC    61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCTCTAGAA   121 CTAGTGGATC TGCCACCATG GAGCAAAAGC TCATTTCTGA AGAGGACTTG AATGAAGGAT   181 CCATGTTCAG CTGGGTGAGC AAGGATGCCC GCCGCAAGAA GGAGCCGGAG CTCTTCCAGA   241 CGGTGGCCGA GGGGCTGCGG CAGCTGTACG CGCAGAAGCT GCTGCCGCTG GAGGAGCACT   301 ATCGCTTCCA CGAGTTCCAC TCGCCCGCGC TGGAGGACGC TGACTTCGAC AACAAGCCGA   361 TGGTGCTCCT GGTCGGCCAG TACAGCACCG GCAAGACCAC CTTCATCCGC CACCTGATCG   421 AGCAGGACTT CCCGGGGATG CGCATCGGGC CGGAGCCCAC CACCGACTCC TTCATCGCGG   481 TCATGCACGG CCCCACCGAG GGCGTGGTGC CCGGCAACGC GCTCGTCGTG GACCCGCGGC   541 GCCCCTTCCG CAAGCTCAAC GCCTTCGGCA ACGCCTTCCT CAACAGGTTC ATGTGTGCAC   601 AGCTGCCCAA CCCAGTACTG GACAGCATCA GCATCATTGA CACTCCTGGG ATCCTGTCTG   661 GGGAGAAGCA GCGCATCAGC CGAGGTTATG ACTTTGCGGC TGTCCTTGAG TGGTTCGCAG   721 AGCGTGTGGA CCGCATCATC TTGTTGTTCG ACGCCCACAA GCTGGACATC TCAGACGAGT   781 TCTCAGAAGT CATCAAGGCC CTCAAAAATC ACGAGGACAA GATCCGTGTG GTGCTGAACA   841 AGGCTGATCA GATCGAGACG CAGCAGCTGA TGCGAGTATA CGGGGCCCTC ATGTGGTCCC   901 TGGGGAAGAT CATCAACACC CCCGAGGTGG TCAGAGTCTA CATCGGCTCC TTCTGGGTCA   961 CACCCACTGC TCATCCCTGA CACCCGGAAA GCTCTTTCGA GGCAGAGGAG CAGGACCTCT  1021 TCAAAGACAT CCAGTCTCTG CCGAGAAACG TCGCCCTCAG AGCTCATGAC CTCATCCAGC  1081 GGTCAGCTGG CCAAGGTCAT GCCTACATCA TCAGCTTCCC TCAGAGGAGA ATGCCCAAAT 1141 GATTTTCC //
Primer: hGCSF_R Sequence file: RDB15386_A7Ibb.seq
>D04916B4_A7Ib_1_hGCSF_R_G07_19_ABI24.ab1     1 GGGGGACTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCGGGAAT    61 TCGATTTAGT CGACTCACTC GTGCCTCCGT TTGGAGGGTG GAATGAGATG TGGAGGAAGG   121 TCAGCGGGCA GCTCGTGGCC CTCTAGCTTC ACCTTGATAA GGTGGTTGGC CAGGGCAAAC   181 TCCTCGTCAT CCAGCAGGCC ATCCTTGTCC ACATCTGCCA ACTTCCAGAT CTTCCCCAGC   241 ACTGTGTTGG GCAGCTTGGA CTTCACCATC TCCTTCTTGG CATTAGCACC TGTGATCTTG   301 CCGTTGACAG GAGACAGTGT GTAGAAGATC TCATCATAGG TGGGCTTGTC CTTGCCAACT   361 ACCCACTCAA CATCATCAAT GCCCTCGCCA GCCCCCTCGC CGTAGCCATG CCCAAAGGGC   421 CCATTCATGG TGCCATCAAA AGCACCACCC TTCACAGCCT GTGAGGGCAT CAGGGACTCC   481 TCCTGGCGCA CCATCACCAT CAGCCGAGCT ATATCGTTGG CCAGCATATC ATCCACTGTA   541 TCCAGCAGCT TGGGCTTCAA GGCCTGGAAC TTGCTGAAGT CCTGGGTCTG CAGGAGTTCC   601 TGCATCTTAC GCAGGCTTGG GAAGTCGCCG GAGGAGATCT GGTGCTCCCG CTCGATCTTC   661 TGGTAGATCT CTCCCAGGTT GTTCACCAGC TCTTTCTTCT TGCTCTCTTT CCCGAAAACA   721 TTGGGCATCT CCTTCTTGAG GGAGCTGATG ATGTAGGCAT GGACCTTGGC CAGCCTGGCC   781 CGCTTGATGA GGTCATTGAG CTTCCTGAGG GCGGCGTTTC TCGGCAGAGA CTGGATGTCT   841 TTGAAGAGGT CCTGCTCCTC TGCCTCGAAG AGCTTCCGGT TGTCAGGGAT GAGCAGTGGG   901 TGTGACCAGA AGGAGCCGAT GTAGACTCTG ACCACCTCGG GGGTGTTGAT GATCTTCCCC   961 AGGGACCACA TGAGGGCCCC GTATACTCGC ATCAGCTGCT GCGTCTCGAT CTGATCAGCC  1021 CTTGTTCAGC ACCACACGGA TCTTGTCCTC GTGATTTTTG AGGGCCTTGA TGACTTTCTG  1081 AGAACTCGTC TGAGATGTCC AGCTTGTTGG GGGCGTTCGA ACCACCAGGA TGGATGCCGG 1141 TCCACACACG CCTCTTGGCG AAACCAACCT TCCAAGGGAA CAAGAGTCGG GCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.03.25

GNP_filter3_RDBDEP_html_210308.pl