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pEGFP-C1-mouse centaurin-b2-N610A/N691A (#RDB15384)

Expression vector of mouse ACAP2, Rab35 binding-deficient mutant N610A/N691A), N-terminal EGFP-tag.

Clone info. Expression vector of mouse centaurin beta2 [ACAP2 in NCBI] Rab35 binding-deficient mutant (N610A/N691A), fused with N-terminal EGFP, CMV promoter. shRNA-resistant in Etoh, K. and Fukuda, M. J. Biol. Chem. 290, 9064-9074, 2015.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Acap2 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Etoh, K. and Fukuda, M., J. Biol. Chem. 290(360): 9064-9074, 2015)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Etoh, K. and Fukuda, M., J. Biol. Chem. 290(360): 9064-9074, 2015)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15384 pEGFP-C1-mouse centaurin-b2-N610A/N691A Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse centaurin-b2-N610A/N691A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15384).

Reference section:

Kobayashi, H., Fukuda, M., Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. J. Cell Sci. 125 (Pt 9): 2235-2243 (2012). PMID 22344257. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kobayashi, H., Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. J. Cell Sci. 125 (Pt 9): 2235-2243 (2012). PMID 22344257. [link to RRC of NBRP]
reference Kanno, E., Comprehensive screening for novel rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs. Traffic 11 (4): 491-507 (2010). PMID 20070612. [link to RRC of NBRP]