Expression vector of mouse ACAP2, Rab35 binding-deficient mutant N610A/N691A), N-terminal EGFP-tag.
Catalog number | RDB15384 |
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Resource name | pEGFP-C1-mouse centaurin-b2-N610A/N691A |
Clone info. | Expression vector of mouse centaurin beta2 [ACAP2 in NCBI] Rab35 binding-deficient mutant (N610A/N691A), fused with N-terminal EGFP, CMV promoter. shRNA-resistant in Etoh, K. and Fukuda, M. J. Biol. Chem. 290, 9064-9074, 2015. Expression was confirmed by the depositor with fluorescence microscope. |
Vector backbone | pEGFP-C1 (plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells). |
Gene/insert name | mouse Acap2 cDNA |
Depositor | Fukuda, Mitsunori | |
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Other clones in our bank | mouse Acap2 (NCBI Gene 78618) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Etoh, K. and Fukuda, M., J. Biol. Chem. 290(360): 9064-9074, 2015) |
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Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
Remarks | Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15384 | pEGFP-C1-mouse centaurin-b2-N610A/N691A | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
original | Kobayashi, H., Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. J. Cell Sci. 125 (Pt 9): 2235-2243 (2012). PMID 22344257. |
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Further references such as user reports and related articles (go to bottom)
Featured content
This clone will be sequenced a portion and digested by restriction enzyme for examination.
Original, user report and related articles
original | Kobayashi, H., Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. J. Cell Sci. 125 (Pt 9): 2235-2243 (2012). PMID 22344257. |
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reference | Kanno, E., Comprehensive screening for novel rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs. Traffic 11 (4): 491-507 (2010). PMID 20070612. |
2019.07.17
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