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pEGFP-C1-mouse centaurin-b2-N610A/N691A

Expression vector of mouse ACAP2, Rab35 binding-deficient mutant N610A/N691A), N-terminal EGFP-tag.

Catalog number RDB15384
Resource name pEGFP-C1-mouse centaurin-b2-N610A/N691A
Clone info. Expression vector of mouse centaurin beta2 [ACAP2 in NCBI] Rab35 binding-deficient mutant (N610A/N691A), fused with N-terminal EGFP, CMV promoter. shRNA-resistant in Etoh, K. and Fukuda, M. J. Biol. Chem. 290, 9064-9074, 2015.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Acap2 cDNA
Depositor|Developer Fukuda, Mitsunori |
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mouse Acap2
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Etoh, K. and Fukuda, M., J. Biol. Chem. 290(360): 9064-9074, 2015)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Etoh, K. and Fukuda, M., J. Biol. Chem. 290(360): 9064-9074, 2015)。
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          RDB15384 pEGFP-C1-mouse centaurin-b2-N610A/N691A Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Kobayashi, H., Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. J. Cell Sci. 125 (Pt 9): 2235-2243 (2012). PMID 22344257.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Kobayashi, H., Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. J. Cell Sci. 125 (Pt 9): 2235-2243 (2012). PMID 22344257.
          reference Kanno, E., Comprehensive screening for novel rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs. Traffic 11 (4): 491-507 (2010). PMID 20070612.

          2023.04.06

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