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pEGFP-C1-mouse centaurin-b2-N610A/N691A

Expression vector of mouse ACAP2, Rab35 binding-deficient mutant N610A/N691A), N-terminal EGFP-tag.

Catalog number RDB15384
Resource name pEGFP-C1-mouse centaurin-b2-N610A/N691A
Clone info. Expression vector of mouse centaurin beta2 [ACAP2 in NCBI] Rab35 binding-deficient mutant (N610A/N691A), fused with N-terminal EGFP, CMV promoter. shRNA-resistant in Etoh, K. and Fukuda, M. J. Biol. Chem. 290, 9064-9074, 2015.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Acap2 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Acap2 (NCBI Gene 78618) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Etoh, K. and Fukuda, M., J. Biol. Chem. 290(360): 9064-9074, 2015)
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Schedule A (GFP-A) [Link]
Remarks
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RDB15384 pEGFP-C1-mouse centaurin-b2-N610A/N691A Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Kobayashi, H., Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. J. Cell Sci. 125 (Pt 9): 2235-2243 (2012). PMID 22344257.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

Original, user report and related articles

original Kobayashi, H., Rab35 regulates Arf6 activity through centaurin-β2 (ACAP2) during neurite outgrowth. J. Cell Sci. 125 (Pt 9): 2235-2243 (2012). PMID 22344257.
reference Kanno, E., Comprehensive screening for novel rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs. Traffic 11 (4): 491-507 (2010). PMID 20070612.

2019.07.17

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