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pEGFP-C1-mouse Rab33B

Expression vector of mouse RAB33B, N-terminal EGFP-tag.

Catalog number RDB15379
Resource name pEGFP-C1-mouse Rab33B
Clone info. Expression vector of mouse RAB33B, fused with N-terminal EGFP, CMV promoter.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab33b cDNA
Reference of nucleotide sequence AB232629 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab33b (NCBI Gene 19338) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006); (Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)
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Schedule A (GFP-A) [Link]
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RDB15379 pEGFP-C1-mouse Rab33B DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Itoh, T., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Visualization of Organelles (English text)
Featured content Rab family protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15379_A7Ibp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Sequence file: RDB15379_A7Ibe.seq check
>D04916C8_A7Ib_1_EGFP-C2_F04_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNANNNN NNNNCNNNTG GTCCTGCTGG ANTTCGTGAC CGCCGCCGGG
   61 NTCACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA GATCCATGAC TTCGGAGATG
  121 GAGTCGTCGC TGGAGGTCAG CTTCTCGTCC AGCTGTGCGG TGTCAGGGGC GTCTGGGTGT
  181 TTGCCTCCCG CCCGCTCCCG CATCTTCAAG ATCATAGTGA TCGGCGACTC GAACGTGGGC
  241 AAGACGTGCC TGACTTACCG CTTCTGCGCC GGCCGCTTCC CCGACCGCAC CGAGGCCACG
  301 ATCGGGGTGG ACTTCCGAGA GCGAGCCGTG GATATTGATG GAGAGCGCAT CAAGATCCAG
  361 TTGTGGGACA CGGCAGGGCA GGAGCGGTTC AGGAAGAGCA TGGTCCAGCA CTACTACAGG
  421 AATGTGCATG CTGTCGTCTT TGTGTATGAC ATGACCAACA TGGCCAGCTT CCACAGCCTG
  481 CCGGCTTGGA TAGAGGAATG CAAACAGCAC TTGCTGGCGA ACGACATACC TCGAATTCTG
  541 GTGGGAAATA AATGTGACTT GAGAAGCGCC ATTCAGGTGC CCACAGACTT GGCACAGAAG
  601 TTTGCTGACA CACACAGTAT GCCTTTGTTT GAGACCTCTG CTAAAAACCC CAATGATAAT
  661 GACCACGTAG AAGCTATATT CATGACCTTA GCTCATAAGC TAAAGAGCCA CAAGCCATTG
  721 ATGCTTAGCC AGCTGCCTGA TAACAGAATT AGCCTGAAAC CTGAGANCAA GCCTGCGGTG
  781 ACTTGCTGGT GCTGAAATCA CTAGTGAATT CGCGGCCGCC TGCANGTCGA CNGTACCGCG
  841 GGCCCNGGAT NCANNNGATC TAGATAACTG ATCATAATCA GCCNTACCAC ATTTGNANAG
901 GTTTTANTTG CTTTAAAAAA CCTNCNNCAN CTCCCCNNNN NNNNNN
//
Primer: SV40polyA_R2
Sequence file: RDB15379_A7Ibf.seq check
>D04916C8_A7Ib_1_SV40polyA_R2_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA GTTNNNNNNN ACNATTGCAT TCATTTTATG
   61 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
  121 GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC
  181 GACCTGCAGG CGGCCGCGAA TTCACTAGTG ATTTCAGCAC CAGCAAGTCA CCGCAGGCTT
  241 GGTCTCAGGT TTCAGGCTAA TTCTGTTATC AGGCAGCTGG CTAAGCATCA ATGGCTTGTG
  301 GCTCTTTAGC TTATGAGCTA AGGTCATGAA TATAGCTTCT ACGTGGTCAT TATCATTGGG
  361 GTTTTTAGCA GAGGTCTCAA ACAAAGGCAT ACTGTGTGTG TCAGCAAACT TCTGTGCCAA
  421 GTCTGTGGGC ACCTGAATGG CGCTTCTCAA GTCACATTTA TTTCCCACCA GAATTCGAGG
  481 TATGTCGTTC GCCAGCAAGT GCTGTTTGCA TTCCTCTATC CAAGCCGGCA GGCTGTGGAA
  541 GCTGGCCATG TTGGTCATGT CATACACAAA GACGACAGCA TGCACATTCC TGTAGTAGTG
  601 CTGGACCATG CTCTTCCTGA ACCGCTCCTG CCCTGCCGTG TCCCACAACT GGATCTTGAT
  661 GCGCTCTCCA TCAATATCCA CGGCTCGCTC TCGGAAGTCC ACCCCGATCG TGGCCTCGGT
  721 GCGGTCGGGG AAGCGGCCGG CGCANAANCG GTAAGTCAGG CACGTCTTGC CCACGTTCNA
  781 GTCGCCGATC ACTATGATCT TGAAGATGCG GGAGCGGGCG GGANGCANAC ACCCAGACNC
  841 CCCTGACACC GCACAGCTGG ACGAGANNCT GACCTNNAGC GACNANTCCA TCNNCNANNT
  901 CATGGATCTG ANTCCNNANN TGNACNGCTC GNCCATGCCN ANAGTGNTCC CGGNGGCGGT
  961 CACNAACNNC NGCNNNNCAT GTGATCGCNC TNCNNGTNGG GGNNTTNGNN CNNGGNGGAN
1021 NGGGNGNNNN NNNANNNNNN GTCGGGNANC NNNNNNGNGN NNNNNNNNN
//
Primer: CMV_Forward
Sequence file: RDB15379_A7Ibg.seq check
>D04916C8_A7Ib_1_CMV_Forward_E04_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNGNNNN NGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG
  121 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
  181 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
  241 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
  301 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG
  361 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
  421 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
  481 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
  541 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
  601 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
  661 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
  721 CTGAGCACCC AGTCCGCCCT GANCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
  781 CTGGAGTTCG TGACCGCCGC CNGGATCACT CTCNGNATGG ACGANCTGTA CAAGTCCGGA
  841 NTCANATCCA TGACTTCNGA NATGGAGTCG NCNCTGGANG NCAGCTTCTC GTCCAGCNNN
  901 GCGGNGTCAN GGGCNNCTGG NNGNTTGCCT CCNNNNNNNT CCCGCATCNT NNAGANCATA
961 NNGNNCNGCN NNNCNNNCNN GGGNNNNNNG TGNNNGANNN NNNNNNN
//
Primer: SV40pro_F_V2
Sequence file: RDB15379_A7Ibh.seq check
>D04916C8_A7Ib_1_SV40pro_F_V2_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN NNNNNNNNNN CGNTNNNNGA GNCAGGATGA
   61 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG
  121 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG
  181 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC
  241 CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT
  301 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA
  361 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG
  421 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA
  481 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT
  541 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG
  601 AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC
  661 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GNCGGCTGGG TGTGGCGGAC
  721 CGCTATCAGG ACATANCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG
  781 GCTGACCGCT NCCTCGNGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC
  841 TATCGCCTTC TTGANNANTT CTTCTGANCG GNACTCTNGG GTTCNAANTG ANNGACCANG
  901 CGNNGCCNNA CCTGNCNTCA CGANANTTNN NATTCCNNCN GCCNCCTTCT ATNAANGGNN
  961 GGGCTNCNNA ATCGTTTTTC CGGGANGNNN NNNNGNATNN NNCNTCCNGC GNNGGGNTNN
1021 CNNGCTNGNN NTNNTNNGNC NNCCNNNNNG GGGNNNNNNN NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, T., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665.
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

2021.04.26

GNP_filter3_RDBDEP_html_210401.pl