RDB15376 - pEF-T7-mouse Atg16L1g

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icon below as well as clone information before placing your order.

pEF-T7-mouse Atg16L1g (#RDB15376)

Expression vector of mouse Atg16L1, N-terminal T7-tag.

Clone info.	Expression vector of mouse autophagy related 16-like 1 (Atg16L1), tagged with N-terminal T7, EF1 promoter.
Comment	Expression was confirmed by the depositor with immunoblotting.
Vector backbone	pEF-T7 (plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	mouse Atg16l1 cDNA
Reference of insert sequence	AB476646 (DDBJ accession number)
Depositor\|Developer	Fukuda, Mitsunori \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)。 MTAに書く付加的使用条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al., Mol. Biol. Cell, 19 (7): 2916-2925, 2008)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15376	pEF-T7-mouse Atg16L1g	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-mouse Atg16L1g was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15376).

Reference section:

Itoh, T., Fujita, N., Kanno, E., Yamamoto, A., Yoshimori, T., Fukuda, M., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15376_A7Ibp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(T7 tag,Atg16L1g 5') Sequence file: RDB15376_A7Iba.seq
>D04916A3_A7Ib_2_EF1a-C_F_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTCNTTCT CAAGCCTCAG ACAGTGGTTC 61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT 121 CGATTCCACC ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGCGGAT CCATGTCGTC 181 GGGCCTGCGC GCCGCAGACT TTCCCCGCTG GAAGCGTCAC ATCGCGGAGG AACTGAGGCG 241 CCGGGACCGA CTGCAGAGGC AGGCGTTCGA GGAGATCATT CTGCAGTATA CCAAGTTGCT 301 GGAAAAGTCA GATCTTCATT CAGTATTGAC CCAGAAACTA CAAGCAGAAA AGCATGACAT 361 GCCAAATAGG CATGAAATAA GTCCTGGACA TGATGGTGCG TGGAATGATA GTCAACTACA 421 AGAAATGGCC CAGTTGAGGA TCAAACACCA GGAAGAGCTG ACCGAACTGC ACAAGAAGCG 481 TGGGGAGTTA GCTCAGTTGG TGATTGACCT GAACAACCAA ATGCAGCAGA AGGACAAGGA 541 GATACAGATG AATGAAGCAA AGATTTCGGA GTATTTACAG ACCATCTCTG ACCTGGAGAC 601 AAACTGCCTG GACCTGCGCA CCAAACTGCA GGACCTCGAG GTAGCCAACC AGACCCTGAA 661 NGANGAGTAT GACGCCCTGC AGATTACTTT TACTGCCCTA GANNAGAAAC TGANGAAAAC 721 TACTGANGAG AACCAGGAAC TGGTCACCAG ATGGATGGCT GANAANGCCC AAGAAGCCNA 781 TCGCCTCAAT GCAGANAATG ANAANGANTC CNGGNNGCGT CAAGCACGGC TGCAGAANGA 841 NCTTGNANAA NCAGCAAANG AACCTCTACC NGNTGAACAN GNNGATGNNN TTNNNGNCNT 901 NNNGGNNGAN NNCNCNNANN NCNCAGNANN ANNNCNNNNN CNNNNNNNNN N //
Primer: hGCSF_R (Pr0031) Region: hG-CSF pA,insert 3'(Atg16L1g 3') Sequence file: RDB15376_A7Ibb.seq
>D04916A3_A7Ib_2_hGCSF_R_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GGGTCCNNGG NGGGGNNCCT CACTCTAGAG CGGCCGCTAT 61 GGTCGACCTG CAGGCGGCCG CGAATTCACT AGTGATTTCA AGGCTGTGCC CACAGCACAG 121 CTCTGCTTCC TTTGTCCACA CTGACAACAT GTAAGCCCGA GGGGGCCCAC GCCACCGCAT 181 TGATAGAAGA GCTGTGCTGT TTTGAAAGAA CCTTCTCCAC TTTCCCTGTC AGCACACTCC 241 AGACATAAAG AGAACCCTCG GCTGAGCCTG CTGCCACGTA ACTGCCATCA GGGCTGAAGA 301 CAACCCGGGT CCAGTCAGAG CCGCATTTGA ATCCAGGCGC ACTGAATGTC TGTTTGACTG 361 CATTTGTCCG GAGGTCGATG ACTTTTAGCA GGTCATCACG GGAGCAGCTC AGGAGCTCAG 421 TTCTCTCAGG GTTTAGGTCC AGAGCAGTGA TCTTCCCTAA CAGTTCCATC TCTCGGACCA 481 CACTCTCTGA CCGGATATCC CAGAAACGAA TTTTCTTGTC AAAATGTCCA CTCATTACAC 541 ATTGTTCAGT GCAAACAATG TCATTGCAGC TGGATCCTGC AAACACTGTT TTTATGCAGA 601 CTTTGCTGCG GAGATCCCAG AGTTTGAGGG TCCGGTCGTG ACTTCCTGAG ACAATCCGTG 661 CATTGTCCAG CAGGAACTTG GCAGANAGGA CTTTCCCGCT GTGGCCTGTG AGTGTGTGCC 721 GTAATCGATA ATCATCNACA GTCCAGATTC GGCTTGCNAA ATCATTTGAA GCTGCTAATA 781 NGTAANCTCC AGCACTATCA AATTCAATGC TTGTAATTCC AGCATTACTG CCNGANAGGG 841 NNCCCTTGAA TTCACATTTA TCTCCNANNG CTNCCCNAAG TTNCACCNNG CGGNNCNNGC 901 NNCCNNNGNN CNNGCAACCN GGANCCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Itoh, T., Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell 19 (7): 2916-2925 (2008). PMID 18448665. [link to RRC of NBRP]

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mouse Atg16l1

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mouse Atg16l1

Reference sequence