Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGL4-JF1_1128-1312

Luciferase reporter of the mouse Ccnb1ip1, nt.1128 to nt.1312, sense orientation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15331_A7Hmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert 5' Sequence file: RDB15331_A7Hma.seq
>D04779A5_A7Hm_1_pGL4-4174F_E04_13_ABI24.ab1     1 ACTGGGTCAG ACTTTCTCTG GCCTAACTGG CCGCAGTCTG GCAGCACATT TTAAAATTAA    61 GAGGGGAACA GGGAGGAGGG GGAGAAGAAG GAGGAAGAAA AAGAAGAAGA GGAGGAGGAA   121 GAGGAAGAAG AGGAGGAGGA AGAGGAAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG   181 GAGGAGGAGG AGGAGATCTT GAACTCAGCC AATCAGGGGC CTCGGCGGCC AAGCTTAGAC   241 ACTAGAGGGT ATATAATGGA AGCTCGACTT CCAGCTTGGC AATCCGGTAC TGTTGGTAAA   301 GCCACCATGG AAGATGCCAA AAACATTAAG AAGGGCCCAG CGCCATTCTA CCCACTCGAA   361 GACGGGACCG CCGGCGAGCA GCTGCACAAA GCCATGAAGC GCTACGCCCT GGTGCCCGGC   421 ACCATCGCCT TTACCGACGC ACATATCGAG GTGGACATTA CCTACGCCGA GTACTTCGAG   481 ATGAGCGTTC GGCTGGCAGA AGCTATGAAG CGCTATGGGC TGAATACAAA CCATCGGATC   541 GTGGTGTGCA GCGAGAATAG CTTGCAGTTC TTCATGCCCG TGTTGGGTGC CCTGTTCATC   601 GGTGTGGCTG TGGCCCCAGC TAACGACATC TACAACGAGC GCGAGCTGCT GAACAGCATG   661 GGCATCAGCC AGCCCACCGT CGTATTCGTG AGCAAGAAAG GGCTGCAAAA GATCCTCAAC   721 GTGCAAAAGA AGCTACCGAT CATACAAAAG ATCATCATCA TGGATAGCAA GACCGACTAC   781 CAGGGCTTCC AAAGCATGTA CACCTTCGTG ACTTCCCATT TGCCACCCGG CTTCAACGAG   841 TACGACTTCG TGCCCGAGAG CTTCGACCGG GACAAAACCA TCGCCCTGAT CATGAACAGT   901 AGTGGCAGTA CCGGATTGCC CAAGGGCGTA GCCCTACCGC ACCGCACCGC TTGTGTCCGA   961 TTCAGTCATG CCCGCGACCC CATCTTCGGC AAACCAGATC ATCCCCGACA CCGCTATCCC  1021 TCAGCGGTGG TGCCAATTTC AACCACGGGC TTTCAGCCAT GGATCACCAC GCTGGGCTAC 1081 TTGATCTGCG CTTCCGGGAT CGTGCTTCAT GGTACCCGCC TTTCCAAAG //
Primer: pGL4-136R Region: insert 3' Sequence file: RDB15331_A7Hmb.seq
>D04779A5_A7Hm_1_pGL4-136R_E05_14_ABI24.ab1     1 GCCTTTTGCT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTG    61 GAAGTCGAGC TTCCATTATA TACCCTCTAG TGTCTAAGCT TGGCCGCCGA GGCCCCTGAT   121 TGGCTGAGTT CAAGATCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCTCCTCCT   181 CTTCTTCCTC TTCCTCCTCC TCTTCTTCCT CTTCCTCCTC CTCTTCTTCT TTTTCTTCCT   241 CCTTCTTCTC CCCCTCCTCC CTGTTCCCCT CTTAATTTTA AAATGTGCTG CCAGACTGCG   301 GCCAGTTAGG CCAGAGAAAT GTTCTGGCAC CTGCACTTGC ACTGGGGACA GCCTATTTTG   361 CTAGTTTGTT TTGTTTCGTT TTGTTTTGAT GGAGAGCGTA TGTTAGTACT ATCGATTCAC   421 ACAAAAAACC AACACACAGA TGTAATGAAA ATAAAGATAT TTTATTGCGG CCTGTCCAAT   481 ACCTCCCGTA CCTTAATATT ACTTACTTAT CCTTGAGAGA CGTACTAGTA ACCCTGATAA   541 ATGCTTCAAT AATATTGAAA AAGGACGAGT ATGAGCATCC AACATTTTCG TGTCGCACTC   601 ATTCCCTTCT TTGCGGCATT TTGCTTGCCT GTTTTTGCAC ACCCCGAAAC GCTGGTGAAA   661 GTAAAAGATG CTGAAGATCA ACTGGGTGCA AGAGTGGGCT ATATCGAACT GGATCTCAAT   721 AGCGGCAAGA TCCTTGAGTC TTTCCGCCCC GAAGAACGAT TCCCGATGAT GAGCACTTTC   781 AAAGTACTGC TATGTGGCGC GGTGTTGTCC CGTATAGACG CCGGGCAAGA GCAGCTTGGG   841 TCGCCGTATA CACTACTCAC AAAACGACTT GGTTGAGTAC TCGCCGGTCA CGGAAAAGCA   901 TCTTACGGAT GGCATGACGG TAAGAGAATT GTGTAGTGCT GCCATTACCA TGAGCGACAA   961 CACCGCGGGC CAACTTTACT TTCTGACAAC GATCGGGAGG CCCTAAAGGA GCTGACTGCA  1021 TTTTCTTCCA TTATATGGGT GATCATGTGA CCCGGGCTTG AACCGCTTGG ACCAGGAGGT 1081 TGAACGAGTC ATTACCCGCA CCGGACGAGG CGGTGAATAC CCAACCGGAA TG //
Primer: pAxCALNL_F1 Region: Luciferase 3' Sequence file: RDB15331_A7Hmc.seq
>D04779A5_A7Hm_1_pAxCALNLF1_C12_09_ABI24.ab1     1 AATAATATAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA    61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC   121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT   181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT   241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC   301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG   361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG   421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG   481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA   541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC   601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT   661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA   721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC   781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG   841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC   901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT   961 GAACATGCCG AAGCCGTGGT GAAATGGCAC CCACGCTGAG GATAGCGGTG TCGGGGATGA  1021 TCTGGTTTGC CGGAGATGGG GTCGCGGGCA TGACTGAGTC CGACACAGCG CTGCGGTGCG 1081 GTACGCTTAC GCCCTGGGCA TCCGGTTACC TGCCCACTTA ACTGAATC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl