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pGL4-JF1_1128-1312 (#RDB15331)

Luciferase reporter of the mouse Ccnb1ip1, nt.1128 to nt.1312, sense orientation.

Clone info. Luciferase reporter of the mouse cyclin B1 interacting protein 1 (ccnb1ip1) promoter/enhancer [nt.1128 to nt.1312, sense orientation] was inserted, see Fig. 2A of Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4.23 [luc2/minP] (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r
Gene/insert name mouse Ccnb1ip1 genomic DNA
Depositor|Developer Yoshida, Wataru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15331 pGL4-JF1_1128-1312 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-JF1_1128-1312 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15331).

Reference section:

Yoshida, W., Tomikawa, J., Inaki, M., Kimura, H., Onodera, M., Hata, K., Nakabayashi, K., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15331_A7Hmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB15331_A7Hma.seq check
>D04779A5_A7Hm_1_pGL4-4174F_E04_13_ABI24.ab1
    1 ACTGGGTCAG ACTTTCTCTG GCCTAACTGG CCGCAGTCTG GCAGCACATT TTAAAATTAA
   61 GAGGGGAACA GGGAGGAGGG GGAGAAGAAG GAGGAAGAAA AAGAAGAAGA GGAGGAGGAA
  121 GAGGAAGAAG AGGAGGAGGA AGAGGAAGAA GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG
  181 GAGGAGGAGG AGGAGATCTT GAACTCAGCC AATCAGGGGC CTCGGCGGCC AAGCTTAGAC
  241 ACTAGAGGGT ATATAATGGA AGCTCGACTT CCAGCTTGGC AATCCGGTAC TGTTGGTAAA
  301 GCCACCATGG AAGATGCCAA AAACATTAAG AAGGGCCCAG CGCCATTCTA CCCACTCGAA
  361 GACGGGACCG CCGGCGAGCA GCTGCACAAA GCCATGAAGC GCTACGCCCT GGTGCCCGGC
  421 ACCATCGCCT TTACCGACGC ACATATCGAG GTGGACATTA CCTACGCCGA GTACTTCGAG
  481 ATGAGCGTTC GGCTGGCAGA AGCTATGAAG CGCTATGGGC TGAATACAAA CCATCGGATC
  541 GTGGTGTGCA GCGAGAATAG CTTGCAGTTC TTCATGCCCG TGTTGGGTGC CCTGTTCATC
  601 GGTGTGGCTG TGGCCCCAGC TAACGACATC TACAACGAGC GCGAGCTGCT GAACAGCATG
  661 GGCATCAGCC AGCCCACCGT CGTATTCGTG AGCAAGAAAG GGCTGCAAAA GATCCTCAAC
  721 GTGCAAAAGA AGCTACCGAT CATACAAAAG ATCATCATCA TGGATAGCAA GACCGACTAC
  781 CAGGGCTTCC AAAGCATGTA CACCTTCGTG ACTTCCCATT TGCCACCCGG CTTCAACGAG
  841 TACGACTTCG TGCCCGAGAG CTTCGACCGG GACAAAACCA TCGCCCTGAT CATGAACAGT
  901 AGTGGCAGTA CCGGATTGCC CAAGGGCGTA GCCCTACCGC ACCGCACCGC TTGTGTCCGA
  961 TTCAGTCATG CCCGCGACCC CATCTTCGGC AAACCAGATC ATCCCCGACA CCGCTATCCC
 1021 TCAGCGGTGG TGCCAATTTC AACCACGGGC TTTCAGCCAT GGATCACCAC GCTGGGCTAC
1081 TTGATCTGCG CTTCCGGGAT CGTGCTTCAT GGTACCCGCC TTTCCAAAG
//
Primer: pGL4-136R ()
Region: insert 3'
Sequence file: RDB15331_A7Hmb.seq check
>D04779A5_A7Hm_1_pGL4-136R_E05_14_ABI24.ab1
    1 GCCTTTTGCT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTG
   61 GAAGTCGAGC TTCCATTATA TACCCTCTAG TGTCTAAGCT TGGCCGCCGA GGCCCCTGAT
  121 TGGCTGAGTT CAAGATCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCTCCTCCT
  181 CTTCTTCCTC TTCCTCCTCC TCTTCTTCCT CTTCCTCCTC CTCTTCTTCT TTTTCTTCCT
  241 CCTTCTTCTC CCCCTCCTCC CTGTTCCCCT CTTAATTTTA AAATGTGCTG CCAGACTGCG
  301 GCCAGTTAGG CCAGAGAAAT GTTCTGGCAC CTGCACTTGC ACTGGGGACA GCCTATTTTG
  361 CTAGTTTGTT TTGTTTCGTT TTGTTTTGAT GGAGAGCGTA TGTTAGTACT ATCGATTCAC
  421 ACAAAAAACC AACACACAGA TGTAATGAAA ATAAAGATAT TTTATTGCGG CCTGTCCAAT
  481 ACCTCCCGTA CCTTAATATT ACTTACTTAT CCTTGAGAGA CGTACTAGTA ACCCTGATAA
  541 ATGCTTCAAT AATATTGAAA AAGGACGAGT ATGAGCATCC AACATTTTCG TGTCGCACTC
  601 ATTCCCTTCT TTGCGGCATT TTGCTTGCCT GTTTTTGCAC ACCCCGAAAC GCTGGTGAAA
  661 GTAAAAGATG CTGAAGATCA ACTGGGTGCA AGAGTGGGCT ATATCGAACT GGATCTCAAT
  721 AGCGGCAAGA TCCTTGAGTC TTTCCGCCCC GAAGAACGAT TCCCGATGAT GAGCACTTTC
  781 AAAGTACTGC TATGTGGCGC GGTGTTGTCC CGTATAGACG CCGGGCAAGA GCAGCTTGGG
  841 TCGCCGTATA CACTACTCAC AAAACGACTT GGTTGAGTAC TCGCCGGTCA CGGAAAAGCA
  901 TCTTACGGAT GGCATGACGG TAAGAGAATT GTGTAGTGCT GCCATTACCA TGAGCGACAA
  961 CACCGCGGGC CAACTTTACT TTCTGACAAC GATCGGGAGG CCCTAAAGGA GCTGACTGCA
 1021 TTTTCTTCCA TTATATGGGT GATCATGTGA CCCGGGCTTG AACCGCTTGG ACCAGGAGGT
1081 TGAACGAGTC ATTACCCGCA CCGGACGAGG CGGTGAATAC CCAACCGGAA TG
//
Primer: pAxCALNL_F1 ()
Region: Luciferase 3'
Sequence file: RDB15331_A7Hmc.seq check
>D04779A5_A7Hm_1_pAxCALNLF1_C12_09_ABI24.ab1
    1 AATAATATAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA
   61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC
  121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT
  181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT
  241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC
  301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG
  361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG
  421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG
  481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA
  541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC
  601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT
  661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA
  721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC
  781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG
  841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC
  901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT
  961 GAACATGCCG AAGCCGTGGT GAAATGGCAC CCACGCTGAG GATAGCGGTG TCGGGGATGA
 1021 TCTGGTTTGC CGGAGATGGG GTCGCGGGCA TGACTGAGTC CGACACAGCG CTGCGGTGCG
1081 GTACGCTTAC GCCCTGGGCA TCCGGTTACC TGCCCACTTA ACTGAATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, W., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]