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pGL4-JF1_886-1349 (#RDB15329)

Luciferase reporter of the mouse Ccnb1ip1, nt.886 to nt.1349, sense orientation.

Clone info. Luciferase reporter of the mouse cyclin B1 interacting protein 1 (ccnb1ip1) promoter/enhancer [nt.886 to nt.1349, sense orientation] was inserted, see Fig. 2A of Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4.23 [luc2/minP] (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r
Gene/insert name mouse Ccnb1ip1 genomic DNA
Depositor|Developer Yoshida, Wataru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15329 pGL4-JF1_886-1349 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-JF1_886-1349 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15329).

Reference section:

Yoshida, W., Tomikawa, J., Inaki, M., Kimura, H., Onodera, M., Hata, K., Nakabayashi, K., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15329_A7Hmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB15329_A7Hma.seq check
>D04779A3_A7Hm_1_pGL4-4174F_C04_07_ABI24.ab1
    1 ACTGGGTCAG ACATTTCTCT GGCTAACTGG CCGCCCTGAA GGCAATTTCT AAGTTGGATA
   61 GTTACAGACT GTTTATAATT GGACCTATTA TTACCTTGCA TAAGTTAGCT ATGTTGAGGT
  121 TGCAAAAGGA CGGGAGGTTA CTCAGGATGG AAACTGTTTG GAATAGAACA AGGACATGCC
  181 CAAACCGTTT CAATTATCTG TGTTTGTTTG TTTTGTGTCA TGATCCTGAA AGAAATTTTG
  241 TGTAGGAGTT CAATCTTAAA ATGAGCGTTG AGTTGCAGTC TGGCAGCACA TTTTAAAATT
  301 AAGAGGGGAA CAGGGAGGAG GGGGAGAAGA AGGAGGAAGA AAAAGAAGAA GAGGAGGAGG
  361 AAGAGGAAGA AGAGGAGGAG GAAGAGGAAG AAGAGGAGGA GGAGGAGGAG GAGGAGGAGG
  421 AGGAGGAGGA GGAGGAGATC TTGAACTCAG CCAATCAGGT TCAGAGGCCC ATTACTTAGG
  481 TAGCTTCCCG TCCTTGGGCC TCGGCGGCCA AGCTTAGACA CTAGAGGGTA TATAATGGAA
  541 GCTCGACTTC CAGCTTGGCA ATCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA
  601 AACATTAAGA AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG
  661 CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA
  721 CATATCGAGG TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA
  781 GCTATGAAGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC
  841 TTGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT
  901 AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC
  961 GTATTCGTGA GCAAGAAAGG GGCTGCAAAA GATCCCTCAA CGTGCAAAAA GAAAGCTACC
 1021 CGATCATACA AAGATCATCA TTCATGGGAA TAGCAAGACC GGACTTACTA GGCTTCGAAA
1081 AGCAATGTAC CAACCATTCG GTTGAACCTT TTC
//
Primer: pGL4-136R ()
Region: insert 3'
Sequence file: RDB15329_A7Hmb.seq check
>D04779A3_A7Hm_1_pGL4-136R_C05_08_ABI24.ab1
    1 GTCTTTAGGC TTTTGGCATC TTCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTG
   61 GAAGTCGAGC TTCCATTATA TACCCTCTAG TGTCTAAGCT TGGCCGCCGA GGCCCAAGGA
  121 CGGGAAGCTA CCTAAGTAAT GGGCCTCTGA ACCTGATTGG CTGAGTTCAA GATCTCCTCC
  181 TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCTCCTCTT CTTCCTCTTC CTCCTCCTCT
  241 TCTTCCTCTT CCTCCTCCTC TTCTTCTTTT TCTTCCTCCT TCTTCTCCCC CTCCTCCCTG
  301 TTCCCCTCTT AATTTTAAAA TGTGCTGCCA GACTGCAACT CAACGCTCAT TTTAAGATTG
  361 AACTCCTACA CAAAATTTCT TTCAGGATCA TGACACAAAA CAAACAAACA CAGATAATTG
  421 AAACGGTTTG GGCATGTCCT TGTTCTATTC CAAACAGTTT CCATCCTGAG TAACCTCCCG
  481 TCCTTTTGCA ACCTCAACAT AGCTAACTTA TGCAAGGTAA TAATAGGTCC AATTATAAAC
  541 AGTCTGTAAC TATCCAACTT AGAAATTGCC TTCAGGGCGG CCAGTTAGGC CAGAGAAATG
  601 TTCTGGCACC TGCACTTGCA CTGGGGACAG CCTATTTTGC TAGTTTGTTT TGTTTCGTTT
  661 TGTTTTGATG GAGAGCGTAT GTTAGTACTA TCGATTCACA CAAAAAACCA ACACACAGAT
  721 GTAATGAAAA TAAAGATATT TTATTGCGGC CTGTCCAATA CCTCCCGTAC CTTAATATTA
  781 CTTACTTATC CTTGAGAGAC GTACTAGTAA CCCTGATAAA TGCTTCAATA ATATTGAAAA
  841 AGGACGAGTA TGAGCATCCA ACATTTTCGT GTCGCACTCA TTCCCTTCTT TGCGGCATTT
  901 TGCTTGCCTG TTTTTGCACA CCCCGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAA
  961 CTGGGTGCAA GAGTGGGGCT ATATCGAACT GGGATCTCAA TAGCGGGCAA GATCCTTTGA
 1021 GTCTTTCCGC CCCGAAGACG ATTTCCCGAT GAATGAAGCA CTTTCAAGTA CTGCCTATGT
1081 GACGCGATGT GGTCCCGTAA TAGGACGCCC GGGCTAAGAA GC
//
Primer: pAxCALNL_F1 ()
Region: Luciferase 3'
Sequence file: RDB15329_A7Hmc.seq check
>D04779A3_A7Hm_1_pAxCALNLF1_F11_17_ABI24.ab1
    1 ATAAGTATCT TATCATGTCT GCTCGAGCGG CCGGCCGCCC CGACTCTAGA ATTATTACAC
   61 GGCGATCTTG CCGCCCTTCT TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA
  121 CTTGCCGGTC AGTCCTTTAG GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT
  181 GGCGGTTGTA ACCTGGCTGG CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC
  241 GTGTTCCAGC ACGACGACTG CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC
  301 GACCCCGGCG TCGAAGATGT TGGGGTGTTG CAGCAGGATG CTCTCCAGTT CGGCTGGGGC
  361 TACCTGGTAG CCCTTGTATT TGATCAGGCT CTTCAGCCGG TCCACGATGA AGAAGTGCTC
  421 GTCCTCGTCC CAGTAGGCGA TGTCGCCGCT GTGCAGCCAG CCGTCCTTGT CGATGAGAGC
  481 GTTTGTAGCC TCGGGGTTGT TAACGTAGCC GCTCATGATC ATGGGGCCAC GGACGCACAG
  541 CTCGCCGCGC TGGTTCACAC CCAGTGTCTT ACCGGTGTCC AAGTCCACCA CCTTAGCCTC
  601 GAAGAAGGGC ACCACCTTGC CTACTGCGCC AGGCTTGTCG TCCCCTTCGG GGGTGATCAG
  661 AATGGCGCTG GTTGTTTCTG TCAGGCCGTA GCCCTGGCGG ATGCCTGGTA GGTGGAAGCG
  721 TTTGGCCACG GCCTCACCTA CCTCCTTGCT GAGCGGCGCC CCGCCGCTGG CGATCTCGTG
  781 CAAGTTGCTT AGGTCGTACT TGTCGATGAG AGTGCTCTTA GCGAAGAAGC TAAATAGTGT
  841 GGGCACCAGC AGGGCAGATT GAATCTTATA GTCTTGCAAG CTGCGCAAGA ATAGCTCCTC
  901 CTCGAAGCGG TACATGAGCA CGACCCGAAA GCCGCAGATC AAGTAGCCCA GCGTGGTGAA
  961 CATGCCGAAA GCCCGTGGTG AAATGGCACC ACGCTGAGGA TAGCGTGTCG GGGAATGATC
 1021 TGGGTTTGCC GAGATGGGGG TCGCGGCATG ACTGGATTCG ACACAGCGTT GCGCTGCGGT
1081 AAGGCTACGT CTCTTGTCAA GTCAGTACTG CCACTACGTT CATGATCAGG GAGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, W., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]