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pEGFP-C1-mouse Ccz1 (#RDB15295)

Expression vector of mouse CCZ1 vacuolar protein trafficking and biogenesis associated (Ccz1).

Clone info. Expression vector of mouse CCZ1 vacuolar protein trafficking and biogenesis associated (Ccz1), fused with N-terminal EGFP, CMV promoter. Recruited to the late-endosomal state of macropinosomes (Fig.5 of Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2011)..
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Ccz1 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016)。

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RDB15295 pEGFP-C1-mouse Ccz1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Ccz1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15295).

Reference section:

Yasuda, S., Morishita, S., Fujita, A., Nanao, T., Wada, N., Waguri, S., Schiavo, G., Fukuda, M., Nakamura, T., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15295_A7Gop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB15295_A7Goa.seq check
>D04773B4_A7Go_2_EGFP-C2_H12_24_ABI24.ab1
    1 TTATACCCAA CGAGAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
   61 CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA TCCATGGCGG CAGCCGCGGC
  121 CGGGCCGGGT GCTTGGGCGG CCCAGGAGAA GCAGTTCCCG CCAGCGCTGC TGAGCTTCTT
  181 CATCTACAAC CCCCGTTTCG GGCCGCGCGA GGGAGAGGAA GAAAATAAAA TTCTGTTTTA
  241 CCATCCAAAT GAAGTAGAAA AGAATGAAAA AATTAGAAAT GTTGGATTAT GTGAAGCAAT
  301 TGTACAGTTT ACAAGGACTT TTAGCCCATC GAAACCTGCA AAATCTTTAC ACACACAGAA
  361 GAACAGACAG TTCTTCAATG AACCAGAAGA AAATTTCTGG ATGGTTATGG TTGTTCGGAA
  421 TCCTATCATC GAGAAGCAAA GTAAAGATGG CAAGGCGGTA GTGGAGTACC AAGAGGAGGA
  481 GCTGTTGGAC AAGGTGTACA GCTCAGTGCT GCAGCAGTGC TACAGCATGT ACAAGCTTTT
  541 CAATGGGACG TTTCTGAAAG CCATGGAAGA TGGAGGCGTC AAGCTCCTAA AGGAAAGATT
  601 AGAGAAATTC TTCCATCGGT ATTTGCAGAC ACTACATTTG CAGTCATGTG ACCTACTTGA
  661 TATTTTTGGT GGAATCAGCT TCTTCCCATT GGATAAAATG ACTTACTTGA AAATCCAGTC
  721 CTTTATTAAC CGAATGGAAG AAAGCCTAAG TGTAGTCAAA TATACAGCAT TCCTCTACAA
  781 TGACCAACTT ATCTGGAGTG GACTAGAGCA AGATGACATG AGAATTTTGT ACAAATACCT
  841 CACCACCTCA CTGTTCCCCA GGCACATCGA ACCTGAGTTG GCGGGAAGGG ACTCCCCAGT
  901 CAGGGCAGAG ATGCCTGGGA ATCTTCAGCA CTATGGAAGA TTTCTTACTG GACCCTTGAA
  961 CCTTAATGAT CCAGAAGCAA AATGCAGATT CCCCAAAATT TTTGTAAATA CAGATGACAC
 1021 GTATGAGAGC TCCATTTAAT TGTTTATAGC CATGAGTGCA GCCGTGTGCT TCATGATGAC
 1081 GCTCACGCCG CTCACGCCTG AATTTCTGCC GAGACTGGAC AGCAATCGAT TGGGTCCCAA
1141 GCCTTAACCG
//
Primer: pAxEF R2 (Pr0059)
Region: SV40pA,insert 3'
Sequence file: RDB15295_A7Gob.seq check
>D04773B4_A7Go_2_pAxEF_R2_F12_18_ABI24.ab1
    1 TGGGTCAAAA TGCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
   61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
  121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
  181 GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT GCAGGCGGCC
  241 GCGAATTCAC TAGTGATTTC AATCCAAGAA GAAGATGTTG TTGAACTGCG TGGCACACAG
  301 TTTCTTCACC TCTTCATTGA CCTCGATCAG GTTCGCATTC TTCTGACTGA GAATCACGTA
  361 CAGCTCCCGC TGGTCAGACT TCTTCCCAAC AACCCAGTAG TCGCTCATGG CCTTCACAAT
  421 GATTTCTTCA TCTTCATCTG CTCTGGTAAA GTCACTGTTG ATGTCCCCTA ATATCTTCAT
  481 TAGATCTGGG TGGACGGATG TGAGTGACAC ACTGGGCGTT TTCCTCATGT GAATTGTACT
  541 TTTCTCTGCT AAATTCATGT GGTTGAAGTA GATGAACTTA AACTGGGGCT CCTTTTCAGA
  601 CCCAGAGATC CTCTTGTTGA TATTAAACTG CTCACAGATG TCAGATGCCA GCACCGTAAG
  661 CTGGGGCCCA ACGATGCTGT CCAGTCTTCG GCAGAAATCC AGCGTGAGCG GCGTGGAGGC
  721 GTCAATCATG AAGCACACGG CTGCACTCAT GGCCTTATAA ACAATTAAAT GGAGCTCTTC
  781 ATACGTGTCA TCTGTATTTA CAAAAATTTT GGGGAATCTG CATTTTGCTT CTGGATCATT
  841 AAGTTCAAGG GTCCAGTAAG AAATCTTCCA TAGTGCTGAA GATTCCCAGG CATCTCTGCC
  901 CTGACTGGGG AGTCCCTTCC CGCCAACTCA GTTCGATGTG CCTGGGGAAC AGTGAGGTGG
  961 TGAGGTATTT GTACAAAATT CTCATGTCAT CTTGCTCTAG TCCACTCCAG ATAAGTTGGG
 1021 TCATTGTAGA GGATGCTGTA TATTTGACTA CACTTAGCTT TCTTCATTCG GTATAAGGAC
 1081 TGGATTTTCC AGTAGTCATT TTTATCATGG GAGAGGCTGA ATCATAAATA TCAAGTAAGT
1141 TCACATGACT GCAATGTAAG TGTTCTTGCA AAATATAC
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5',insert 5'
Sequence file: RDB15295_A7Goc.seq check
>D04773B4_A7Go_2_CMV-Forward_G12_21_ABI24.ab1
    1 TGTTACAACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
  121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
  601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
  661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
  721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
  781 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT
  841 CAGATCCATG GCGGCAGCCG CGGCCGGGCC GGGTGCTTGG GCGGCCCAGG AGAAGCAGTT
  901 CCCGCCAGCG CTGCTGAGCT TCTTCATCTA CAACCCCCGT TTCGGGCCGC GCGAGGGAGA
  961 GGAAGAAAAT AAAATTCTGT TTTACCATCC AAATGAAGTA GAAAAGAATG AAAAAAATTA
 1021 GAAATGTGGG ATTATGTGAA AGCAAATTGT ACAGTTTACA AGGAACTTTT AGCCCAATCG
 1081 GAATCTGCAA AATCTTTACA CACACAGAGG ACCAAACAGT CCTCAATGAC CAGAAAGAAA
1141 AATTTCTTGG GAATGGGTTA TAGGGGATG
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB15295_A7God.seq check
>D04773B4_A7Go_2_SV40pro_F_V2_H12_24_ABI24.ab1
    1 AACGAGCTTT TTGAGGCCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC
  901 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG
  961 ATCGTTTTCC GGGACGCCGG CTGGATGATC TCAGCGCGGG GATCTCATGC TGGAGTCTCG
 1021 CCCACCCTAG GGGAGGCTAC TGAACACGAT GAGACATACT GAGACCCGCG CTATGACGCA
1081 TTAAAGGACG GATACGCACG TGATGATCGT GTCCATAACG CGGGGTTTCG GATCCAAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yasuda, S., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821. [link to RRC of NBRP]