Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-mouse Ccz1

Expression vector of mouse CCZ1 vacuolar protein trafficking and biogenesis associated (Ccz1).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016).

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15295_A7Gop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 Region: EGFP 3',insert 5' Sequence file: RDB15295_A7Goa.seq
>D04773B4_A7Go_2_EGFP-C2_H12_24_ABI24.ab1     1 TTATACCCAA CGAGAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT    61 CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA TCCATGGCGG CAGCCGCGGC   121 CGGGCCGGGT GCTTGGGCGG CCCAGGAGAA GCAGTTCCCG CCAGCGCTGC TGAGCTTCTT   181 CATCTACAAC CCCCGTTTCG GGCCGCGCGA GGGAGAGGAA GAAAATAAAA TTCTGTTTTA   241 CCATCCAAAT GAAGTAGAAA AGAATGAAAA AATTAGAAAT GTTGGATTAT GTGAAGCAAT   301 TGTACAGTTT ACAAGGACTT TTAGCCCATC GAAACCTGCA AAATCTTTAC ACACACAGAA   361 GAACAGACAG TTCTTCAATG AACCAGAAGA AAATTTCTGG ATGGTTATGG TTGTTCGGAA   421 TCCTATCATC GAGAAGCAAA GTAAAGATGG CAAGGCGGTA GTGGAGTACC AAGAGGAGGA   481 GCTGTTGGAC AAGGTGTACA GCTCAGTGCT GCAGCAGTGC TACAGCATGT ACAAGCTTTT   541 CAATGGGACG TTTCTGAAAG CCATGGAAGA TGGAGGCGTC AAGCTCCTAA AGGAAAGATT   601 AGAGAAATTC TTCCATCGGT ATTTGCAGAC ACTACATTTG CAGTCATGTG ACCTACTTGA   661 TATTTTTGGT GGAATCAGCT TCTTCCCATT GGATAAAATG ACTTACTTGA AAATCCAGTC   721 CTTTATTAAC CGAATGGAAG AAAGCCTAAG TGTAGTCAAA TATACAGCAT TCCTCTACAA   781 TGACCAACTT ATCTGGAGTG GACTAGAGCA AGATGACATG AGAATTTTGT ACAAATACCT   841 CACCACCTCA CTGTTCCCCA GGCACATCGA ACCTGAGTTG GCGGGAAGGG ACTCCCCAGT   901 CAGGGCAGAG ATGCCTGGGA ATCTTCAGCA CTATGGAAGA TTTCTTACTG GACCCTTGAA   961 CCTTAATGAT CCAGAAGCAA AATGCAGATT CCCCAAAATT TTTGTAAATA CAGATGACAC  1021 GTATGAGAGC TCCATTTAAT TGTTTATAGC CATGAGTGCA GCCGTGTGCT TCATGATGAC  1081 GCTCACGCCG CTCACGCCTG AATTTCTGCC GAGACTGGAC AGCAATCGAT TGGGTCCCAA 1141 GCCTTAACCG //
Primer: pAxEF R2 Region: SV40pA,insert 3' Sequence file: RDB15295_A7Gob.seq
>D04773B4_A7Go_2_pAxEF_R2_F12_18_ABI24.ab1     1 TGGGTCAAAA TGCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA    61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG   121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT   181 GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT GCAGGCGGCC   241 GCGAATTCAC TAGTGATTTC AATCCAAGAA GAAGATGTTG TTGAACTGCG TGGCACACAG   301 TTTCTTCACC TCTTCATTGA CCTCGATCAG GTTCGCATTC TTCTGACTGA GAATCACGTA   361 CAGCTCCCGC TGGTCAGACT TCTTCCCAAC AACCCAGTAG TCGCTCATGG CCTTCACAAT   421 GATTTCTTCA TCTTCATCTG CTCTGGTAAA GTCACTGTTG ATGTCCCCTA ATATCTTCAT   481 TAGATCTGGG TGGACGGATG TGAGTGACAC ACTGGGCGTT TTCCTCATGT GAATTGTACT   541 TTTCTCTGCT AAATTCATGT GGTTGAAGTA GATGAACTTA AACTGGGGCT CCTTTTCAGA   601 CCCAGAGATC CTCTTGTTGA TATTAAACTG CTCACAGATG TCAGATGCCA GCACCGTAAG   661 CTGGGGCCCA ACGATGCTGT CCAGTCTTCG GCAGAAATCC AGCGTGAGCG GCGTGGAGGC   721 GTCAATCATG AAGCACACGG CTGCACTCAT GGCCTTATAA ACAATTAAAT GGAGCTCTTC   781 ATACGTGTCA TCTGTATTTA CAAAAATTTT GGGGAATCTG CATTTTGCTT CTGGATCATT   841 AAGTTCAAGG GTCCAGTAAG AAATCTTCCA TAGTGCTGAA GATTCCCAGG CATCTCTGCC   901 CTGACTGGGG AGTCCCTTCC CGCCAACTCA GTTCGATGTG CCTGGGGAAC AGTGAGGTGG   961 TGAGGTATTT GTACAAAATT CTCATGTCAT CTTGCTCTAG TCCACTCCAG ATAAGTTGGG  1021 TCATTGTAGA GGATGCTGTA TATTTGACTA CACTTAGCTT TCTTCATTCG GTATAAGGAC  1081 TGGATTTTCC AGTAGTCATT TTTATCATGG GAGAGGCTGA ATCATAAATA TCAAGTAAGT 1141 TCACATGACT GCAATGTAAG TGTTCTTGCA AAATATAC //
Primer: CMV_Forward Region: CMV pro,EGFP 5',insert 5' Sequence file: RDB15295_A7Goc.seq
>D04773B4_A7Go_2_CMV-Forward_G12_21_ABI24.ab1     1 TGTTACAACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT   121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA   181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA   241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT   301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA   361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA   421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA   481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT   541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT   601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA   661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT   721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT   781 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT   841 CAGATCCATG GCGGCAGCCG CGGCCGGGCC GGGTGCTTGG GCGGCCCAGG AGAAGCAGTT   901 CCCGCCAGCG CTGCTGAGCT TCTTCATCTA CAACCCCCGT TTCGGGCCGC GCGAGGGAGA   961 GGAAGAAAAT AAAATTCTGT TTTACCATCC AAATGAAGTA GAAAAGAATG AAAAAAATTA  1021 GAAATGTGGG ATTATGTGAA AGCAAATTGT ACAGTTTACA AGGAACTTTT AGCCCAATCG  1081 GAATCTGCAA AATCTTTACA CACACAGAGG ACCAAACAGT CCTCAATGAC CAGAAAGAAA 1141 AATTTCTTGG GAATGGGTTA TAGGGGATG //
Primer: SV40pro_F_V2 Region: NeoR_KanR Sequence file: RDB15295_A7God.seq
>D04773B4_A7Go_2_SV40pro_F_V2_H12_24_ABI24.ab1     1 AACGAGCTTT TTGAGGCCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT    61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC   121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC   181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG   241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG   301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG   361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG   421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC   481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG   541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC   601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG   661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC   721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC   781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC   841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC   901 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG   961 ATCGTTTTCC GGGACGCCGG CTGGATGATC TCAGCGCGGG GATCTCATGC TGGAGTCTCG  1021 CCCACCCTAG GGGAGGCTAC TGAACACGAT GAGACATACT GAGACCCGCG CTATGACGCA 1081 TTAAAGGACG GATACGCACG TGATGATCGT GTCCATAACG CGGGGTTTCG GATCCAAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl