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pEGFP-C1-mouse Mon1a (#RDB15294)

Expression vector of mouse MON1 homolog A, secretory traffciking associated (Mon1a).

Clone info. Expression vector of mouse MON1 homolog A, secretory traffciking associated (Mon1a), fused with N-terminal EGFP, CMV promoter. Recruited to the late-endosomal state of macropinosomes (Fig.5 of Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2011)..
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Mon1a cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016).
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15294 pEGFP-C1-mouse Mon1a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse Mon1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15294).

Reference section:

Yasuda, S., Morishita, S., Fujita, A., Nanao, T., Wada, N., Waguri, S., Schiavo, G., Fukuda, M., Nakamura, T., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15294_A7Gop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert 5'
Sequence file: RDB15294_A7Goa.seq check
>D04773B3_A7Go_1_EGFP-C2_E12_15_ABI24.ab1
    1 TACCACCACG AGAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TATGGCTGCT GACATGCAGA
  121 GGAAGAGAAG CAGCGAATGC CCCGAGGGCA CACTGGCTCC TTCCAATGGC CAAAGTGTGG
  181 AAAGAGCTGA GAGCCCCACG CCAGGACTAA CCCAGGGAAC GGAGCCAGGT GCTGGGCAGG
  241 AGGGTGCGAT GTTCGTCCAT ACACGTTCCT ATGAAGACCT GACAGAGTTA GAAGACAGAG
  301 AAGCTTCTGG GGACAGCCCC AAAGAGTGCG TTGGGAGTCC CCCACCACTG GCTACGGACA
  361 TGCGCCAGAT TAGCCAGGAC TTCAGCGAGC TAAGCACCCA GCTGACCGGT GTGGCCCGGG
  421 ACCTGCAGGA GGAAATGCTG CCAGGAAGCT CTGAAGACTG GCCGGAGCCC CAGGGCGCAG
  481 CTGGCCGAGG AGCAGCCACA GAGCCCTCCC AAGAAGGCTC AACTGAAGGG GAAGAGGAAG
  541 ACGCTACGGA GGCATGGCGT CTCCACCAGA AGCATGTGTT TGTACTGAGT GAGGCAGGGA
  601 AGCCTGTGTA TTCTCGCTAT GGGTCTGAGG AAGCCCTTTC CAGCACTATG GGTGTCATGG
  661 TTGCCCTGGT GTCCTTCCTG GAGGCAGACA AGAATGCCAT CCGCTCCATC CATGCAGATG
  721 GCTACAAGGT AGTATTCGTG CGCCGGAGCC CTCTGGTGCT GGTGGCGGTG GCCCGCACGC
  781 GGCAGTCGGC CCAGGAGCTG GCGCAGAGCT ACTGTACATC TACTACCAGA TCCTGAGCCT
  841 TCTTACCGGC GCGCAGCTGA GCCACATCTT CCAGCAGAAG CAGAACTACG ACCTGCGGCG
  901 CCTGCTCTCG GGCTCGGAGC GAATCACCGA TAACCTGCTG CAGCTAATGG CTCGAGACCC
  961 CAGTTTCCTG ATGGGCGCGG CGCGGTGCCT GCCTTTGGCA GCGGCCGTGC GGGACACGGT
 1021 GAGTGCAGCC TGCAGCAGGC GCGTGCTCGC AGCTGTCTTT TCATCTGCTG ACCATTACAG
 1081 CCTGTTGCGC TCGGTGCGCC GCAAGAACAA TTCTCAATTC AATCGACTGC ACTGGCCTTT
1141 TCCACCCTCT CATTTAGA
//
Primer: pAxEF R2 (Pr0059)
Region: SV40pA,insert 3'
Sequence file: RDB15294_A7Gob.seq check
>D04773B3_A7Go_1_pAxEF_R2_G06_07_ABI08.ab1
    1 TAGGGAAAAA AGGGCCTTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA
   61 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC
  121 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  181 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACCTGCAGG
  241 CGGCCGCGAA TTCACTAGTG ATTTCAATAG GTGAGGGGCG TGAGGATGAA GAGACGGTCT
  301 TCCTCTTTAC GGATCCAGCG CATCAGCTTA TGGATGGCGC TGACAGCTGA TGCCTTGGTC
  361 CCCAGTGGGC TGTAGCACAT GTAGAGTTCA AAGGCACCTG TCACCCAGGC TAGGAGGTTC
  421 TCATTGGGGC CTGTGTAGTA GATGGTCTTG AGTGGGCGAG AGGCATTGTG GGCACGGCTA
  481 TGCAGGTACT GGTAGAGGCC CAACAGTCGC TCCTGCTCCT CCTCACTGGA GTACGGGGCC
  541 TCAATCTCAG GACTGGTGAA GAGTCCTGAG CTCTTTGATT TATAGAGGAA GTGGCGAAGG
  601 TCAGGGATGC CCACTTGGGC AACGCTGTAG TAAGGTGTGC GCAGCGCCTC CCGCAGGGCC
  661 AAATGGGTTC CCCGCTTTCG GAGGCGTTCC TGGAAGCGCC GTCGGCAGTC AGAGACTGCA
  721 AAGAAGTCCT CACGGTCAGT GGAGATCAGC AGCAGGCAGA GGTCGGTGTC AGGTTCTAGG
  781 TAAGAGATGT GTGCGTGGAA GAAGCCGGCC GCATTGAACT TTGGCAGGCA CACTGGGGGT
  841 CCAAGCCCTC GCCCTCACGG GAAGGAGGAG GAGGAGCTAA TGAGGTTGAA AAAGCAGGTG
  901 CAGGTCGATG GGATGAAAGG AATTGGTCCT TGCGGCGCAC GAGCGCCACC AGCTGGTTAT
  961 GGGCCAAGCA GGATGGGAAA AAGACCAAGG CTGCGAGCAC GCGCCCTGCT GCAAGCTGGG
 1021 CCACTTCACG TGTCCCGGCA ACGATCGCTG CCTAAAGCAA GGCAACGCGT CGTCACCAAT
1081 CCAGAAACTG GGGGATCCTC CGGAAGCTCA GATTAAGAGC
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB15294_A7Goc.seq check
>D04773B3_A7Go_1_CMV-Forward_H06_08_ABI08.ab1
    1 CGATATAACT TCCGGCCCCC ATTGGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
  601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
  661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
  721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
  781 CTGCTGGAGT TCGTGACCGC CGCCGGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC
  841 CGGGACTCAG ATCTATGGCT GCTGACATGC AGAGGAAGAG AAAGCAGCGA ATGCCCCCGA
  901 GGGCACACTG GGCTCCTTCC AAATGACCAA AGTGTGGAAA GAGCCTGAGA GCCCCCACGC
  961 CAGGGACTAA CCCCAGGGGA AACGGGAGCC AGGTGCTGGG GCAAGGGAGG ATGCGGATGT
 1021 TTCGTCCATA CAACGTTTCC CTAATGAAGA ACCTGACCAG CAGTAGAAGT ACAGAAGAAG
1081 GCTTTCCTGG GGGGGAAC
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB15294_A7God.seq check
>D04773B3_A7Go_1_SV40pro_F_V2_D12_12_ABI24.ab1
    1 AGGAAGCTTT TTGGAGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
  901 AACCCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT GGGCTTCGGA
  961 ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGGC GGGGGATCTC ATGCTGGAGT
 1021 TCTTCGCCCA CCCTAGGGGA GCTAACTGAA ACACGGATGG AGACAATACC GGAGACCCGC
 1081 GCTATGACGC AATAAAGAAC GATAAACGCA CGGTGTTGGA TCGTTGCTCA TAACGCGGGT
1141 TCGCTTCCCA GGGCTTGTGG G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yasuda, S., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821. [link to RRC of NBRP]