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pEGFP-C1-mouse Mon1a

Expression vector of mouse MON1 homolog A, secretory traffciking associated (Mon1a).

Catalog number RDB15294
Resource name pEGFP-C1-mouse Mon1a
Clone info. Expression vector of mouse MON1 homolog A, secretory traffciking associated (Mon1a), fused with N-terminal EGFP, CMV promoter. Recruited to the late-endosomal state of macropinosomes (Fig.5 of Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2011)..
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Mon1a cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Mon1a

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016)。
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          RDB15294 pEGFP-C1-mouse Mon1a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Yasuda, S., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15294_A7Gop1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP 3',insert 5'
          Sequence file: RDB15294_A7Goa.seq check
          >D04773B3_A7Go_1_EGFP-C2_E12_15_ABI24.ab1
              1 TACCACCACG AGAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
             61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TATGGCTGCT GACATGCAGA
            121 GGAAGAGAAG CAGCGAATGC CCCGAGGGCA CACTGGCTCC TTCCAATGGC CAAAGTGTGG
            181 AAAGAGCTGA GAGCCCCACG CCAGGACTAA CCCAGGGAAC GGAGCCAGGT GCTGGGCAGG
            241 AGGGTGCGAT GTTCGTCCAT ACACGTTCCT ATGAAGACCT GACAGAGTTA GAAGACAGAG
            301 AAGCTTCTGG GGACAGCCCC AAAGAGTGCG TTGGGAGTCC CCCACCACTG GCTACGGACA
            361 TGCGCCAGAT TAGCCAGGAC TTCAGCGAGC TAAGCACCCA GCTGACCGGT GTGGCCCGGG
            421 ACCTGCAGGA GGAAATGCTG CCAGGAAGCT CTGAAGACTG GCCGGAGCCC CAGGGCGCAG
            481 CTGGCCGAGG AGCAGCCACA GAGCCCTCCC AAGAAGGCTC AACTGAAGGG GAAGAGGAAG
            541 ACGCTACGGA GGCATGGCGT CTCCACCAGA AGCATGTGTT TGTACTGAGT GAGGCAGGGA
            601 AGCCTGTGTA TTCTCGCTAT GGGTCTGAGG AAGCCCTTTC CAGCACTATG GGTGTCATGG
            661 TTGCCCTGGT GTCCTTCCTG GAGGCAGACA AGAATGCCAT CCGCTCCATC CATGCAGATG
            721 GCTACAAGGT AGTATTCGTG CGCCGGAGCC CTCTGGTGCT GGTGGCGGTG GCCCGCACGC
            781 GGCAGTCGGC CCAGGAGCTG GCGCAGAGCT ACTGTACATC TACTACCAGA TCCTGAGCCT
            841 TCTTACCGGC GCGCAGCTGA GCCACATCTT CCAGCAGAAG CAGAACTACG ACCTGCGGCG
            901 CCTGCTCTCG GGCTCGGAGC GAATCACCGA TAACCTGCTG CAGCTAATGG CTCGAGACCC
            961 CAGTTTCCTG ATGGGCGCGG CGCGGTGCCT GCCTTTGGCA GCGGCCGTGC GGGACACGGT
           1021 GAGTGCAGCC TGCAGCAGGC GCGTGCTCGC AGCTGTCTTT TCATCTGCTG ACCATTACAG
           1081 CCTGTTGCGC TCGGTGCGCC GCAAGAACAA TTCTCAATTC AATCGACTGC ACTGGCCTTT
          1141 TCCACCCTCT CATTTAGA
          //
          Primer: pAxEF R2 (Pr0059)
          Region: SV40pA,insert 3'
          Sequence file: RDB15294_A7Gob.seq check
          >D04773B3_A7Go_1_pAxEF_R2_G06_07_ABI08.ab1
              1 TAGGGAAAAA AGGGCCTTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA
             61 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC
            121 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
            181 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACCTGCAGG
            241 CGGCCGCGAA TTCACTAGTG ATTTCAATAG GTGAGGGGCG TGAGGATGAA GAGACGGTCT
            301 TCCTCTTTAC GGATCCAGCG CATCAGCTTA TGGATGGCGC TGACAGCTGA TGCCTTGGTC
            361 CCCAGTGGGC TGTAGCACAT GTAGAGTTCA AAGGCACCTG TCACCCAGGC TAGGAGGTTC
            421 TCATTGGGGC CTGTGTAGTA GATGGTCTTG AGTGGGCGAG AGGCATTGTG GGCACGGCTA
            481 TGCAGGTACT GGTAGAGGCC CAACAGTCGC TCCTGCTCCT CCTCACTGGA GTACGGGGCC
            541 TCAATCTCAG GACTGGTGAA GAGTCCTGAG CTCTTTGATT TATAGAGGAA GTGGCGAAGG
            601 TCAGGGATGC CCACTTGGGC AACGCTGTAG TAAGGTGTGC GCAGCGCCTC CCGCAGGGCC
            661 AAATGGGTTC CCCGCTTTCG GAGGCGTTCC TGGAAGCGCC GTCGGCAGTC AGAGACTGCA
            721 AAGAAGTCCT CACGGTCAGT GGAGATCAGC AGCAGGCAGA GGTCGGTGTC AGGTTCTAGG
            781 TAAGAGATGT GTGCGTGGAA GAAGCCGGCC GCATTGAACT TTGGCAGGCA CACTGGGGGT
            841 CCAAGCCCTC GCCCTCACGG GAAGGAGGAG GAGGAGCTAA TGAGGTTGAA AAAGCAGGTG
            901 CAGGTCGATG GGATGAAAGG AATTGGTCCT TGCGGCGCAC GAGCGCCACC AGCTGGTTAT
            961 GGGCCAAGCA GGATGGGAAA AAGACCAAGG CTGCGAGCAC GCGCCCTGCT GCAAGCTGGG
           1021 CCACTTCACG TGTCCCGGCA ACGATCGCTG CCTAAAGCAA GGCAACGCGT CGTCACCAAT
          1081 CCAGAAACTG GGGGATCCTC CGGAAGCTCA GATTAAGAGC
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,EGFP 5'
          Sequence file: RDB15294_A7Goc.seq check
          >D04773B3_A7Go_1_CMV-Forward_H06_08_ABI08.ab1
              1 CGATATAACT TCCGGCCCCC ATTGGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
            121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
            181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
            241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
            301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
            361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
            421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
            481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
            541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
            601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
            661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
            721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
            781 CTGCTGGAGT TCGTGACCGC CGCCGGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC
            841 CGGGACTCAG ATCTATGGCT GCTGACATGC AGAGGAAGAG AAAGCAGCGA ATGCCCCCGA
            901 GGGCACACTG GGCTCCTTCC AAATGACCAA AGTGTGGAAA GAGCCTGAGA GCCCCCACGC
            961 CAGGGACTAA CCCCAGGGGA AACGGGAGCC AGGTGCTGGG GCAAGGGAGG ATGCGGATGT
           1021 TTCGTCCATA CAACGTTTCC CTAATGAAGA ACCTGACCAG CAGTAGAAGT ACAGAAGAAG
          1081 GCTTTCCTGG GGGGGAAC
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB15294_A7God.seq check
          >D04773B3_A7Go_1_SV40pro_F_V2_D12_12_ABI24.ab1
              1 AGGAAGCTTT TTGGAGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT
             61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
            121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
            181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
            241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
            301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
            361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
            421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
            481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
            541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
            601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
            661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
            721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
            781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
            841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
            901 AACCCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT GGGCTTCGGA
            961 ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGGC GGGGGATCTC ATGCTGGAGT
           1021 TCTTCGCCCA CCCTAGGGGA GCTAACTGAA ACACGGATGG AGACAATACC GGAGACCCGC
           1081 GCTATGACGC AATAAAGAAC GATAAACGCA CGGTGTTGGA TCGTTGCTCA TAACGCGGGT
          1141 TCGCTTCCCA GGGCTTGTGG G
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yasuda, S., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821.

          2023.04.06

          GNP_filter3_RDBDEP_html_230405.pl