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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse myosin Vb-tail (#RDB15286)

Expression vector of mouse myosin Vb tail region (Myo5b).

Clone info. Expression vector of mouse myosin Vb tail region [Myo5b in NCBI], tagged with N-terminal FLAG, EF1 promoter. ｃDNA fragment 3853nt to 5619nt of NM_201600.2 is cloned.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : G to A substitution at 4419nt of NM_201600.2 (Arg, Synonym).
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo5b cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Swiatecka-Urban, A. et al., J. Biol. Chem. 282 (32): 23725-23736, 2007).
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Swiatecka-Urban, A. et al., J. Biol. Chem. 282 (32): 23725-23736, 2007)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15286 pEF-FLAG-mouse myosin Vb-tail DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse myosin Vb-tail was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15286).

Reference section:

Swiatecka-Urban, A., Talebian, L., Kanno, E., Moreau-Marquis, S., Coutermarsh, B., Hansen, K., Karlson, K.H., Barnaby, R., Cheney, R.E., Langford, G.M., Fukuda, M., Stanton, B.A., Myosin Vb is required for trafficking of the cystic fibrosis transmembrane conductance regulator in Rab11a-specific apical recycling endosomes in polarized human airway epithelial cells. J. Biol. Chem. 282 (32): 23725-23736 (2007). PMID 17462998. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15286_A7Gop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB15286_A7Goa.seq check
>D04773A4_A7Go_1_EF1a-C_F_D07_10_ABI24.ab1
    1 CCATTAATGA CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC
  121 GATTCCACCA TGGACTACAA GGACGATGAC GACAAGGGAT CCAGCTCCCC AGACAGCTAC
  181 AGCCTCCTCC TGAACCAGCT CAAGCTGGCC AATGAGGAGC TCGAGGTCCG CAAAGAGGAG
  241 GTGCTGATCC TCAGGACCCA GATCATGAAT GCCGACCAGC GCCGCCTGTC TGGCAAGAAC
  301 ATGGAGCCGA ACATCAATGC CAGAACAAGT TGGCCCAACA GTGAGAAGCA CGTGGACCAG
  361 GAAGACGCCA TTGAGGCCTA TCACGGGGTC TGCCAGACAA ACAGGTTGCT GGAGGCCCAG
  421 CTGCAGGCCC AGAGCCTGGA GCATGAGGAG GAGGTGGAAC ATCTCAAGGC CCAGGTGGAA
  481 GCCCTGAAAG AGGAGATGGA CAAACAGCAG CAGACCTTCT GCCAGACCCT GCTGCTCTCC
  541 CCAGAGGCCC AGGTAGAATT TGGTGTCCAG CAGGAGATAT CCCGGCTGAC CAATGAGAAC
  601 CTGGATTTTA AGGAATTGGT GGAAAAGCTG GAGAAGAATG AGAGGAAGCT GAAGAAGCAG
  661 CTGAAGATTT ACATGAAGAA GGTCCAGGAC TTAGAAGCTG CCCAGGCGTT GGCACAGAGT
  721 GACAGGAGGC ACCATGAACT CACAAGACAG GTCACAGTCC AACGAAAAGA GAAGGACTTC
  781 CAAGGCATGC TGGAGTACCA CAAAGAGGAC GAAGCCCTCC TCATCCGGAA CCTGGTGACA
  841 GACCTGAAGC CTCAGATGCT GTCGGGCACC GTGCCCTGTC TGCCTGCATA CATACTCTAT
  901 ATGTGCATCA CGCACGCGGA TTACACCAAC GATGACCTCA AAGGTGCACT CGTTGCTGAG
  961 CTCCACCATC AACGGCATTA AGAAAGTCCT CAAGAAGCAC AATGATGACT TTGAGATGAA
 1021 CGTCAATTCT GGCTATCCCA ACACTTGGCG GCTCGTCACT GTCTGAAGCA TTACAGTGAT
1081 TGAATGAGGG ATTTTCAATG GAACACAG
//
Primer: hCGSF_R
Sequence file: RDB15286_A7Gob.seq check
>D04773A4_A7Go_1_hGCSF_R_D08_11_ABI24.ab1
    1 CTGCTCTCAT CTCTCAGGGT CCCAGGTGGG GACCCTCACT CTAGAGCGGC CGCTATGGTC
   61 GACCTGCAGG CGGCCGCGAA TTCACTAGTG ATTTCAGACT TCATTGAGAA ACTCCAGGTT
  121 GAGACAGGCC GGGATGTGGA TCGAGTCCAT GGTCAGAGCA GATGGGTTAT ATGGAAACAG
  181 AACTGGGAAC ACGTGCTTGG AGTCCAGCAG GAGCTGCTGA GGGTCATTCC TCTCTTGTAG
  241 CTGAGCCTGG ATTGTTCGTA TGAAGGACAC TGTGACCCGT TCCTCAAATT CATTCAAGGG
  301 AGTGTAGAGG TTTAAAATTT TGACAATCTG CTGGGTGCTG AGGGAGGTGC ACAGAGAGCA
  361 GATGGCCTCA GCATCCTCGT GGGTTTTCTT CTTCAGCTGG AGGAGCTGGG CTGCCTGGAT
  421 GAGGGGCTCC ATGGTCTGAA CTGCTCCACT TTGGTGAAGG TTTTTGCCCC GAAGCCACTC
  481 TTCCAGTTGA CTTATGTTGT ACCTGAGTTG CATGCCTGTG CTCCAGGAGC AGGCGTCTTT
  541 CCGCAGGAGT AGGTTGTTAA GAGTCACAGC GTTGATCATG TAGAAGAGCT GTTTGAACAC
  601 CTGCAGGATA ATCTCGGGGT CCAGACCCTG GTCACACAGG ACTGTATGGA AAGAATTCAT
  661 CTGGCGGACG ATGGCCTCCA GGCAATACGA ATTCTCTCCA TCCACCATGC TGGAGGAGCG
  721 CTTCCGGTAA CCAGTTGGTC TCACACCAGA CAGCCCCTGG ATACTCTCAT TTTCCAACAT
  781 GGCAGAAACT ATCATAGGCT GCAGCAAGCC CTCGGCGATT TTAATGAGCT GCTGATAGAT
  841 CTGAATGGAA AGGTCGCTTA GCACCTGGCG GTATTCAGTG AGGTCAAAGT TCTTGAGACA
  901 GTGCTCATTC TGCTTCGCAG TGTTCTGTGT CATGAAACCC TCATCACCAC TGTATTGCTT
  961 CAGACAGTGA AGGAAGCGGC AGGTGTTGGG ATAGCCCAGA ATGACGTCAT CTCAAGTCAT
 1021 CCATTGTGCT TCTTGAGGAC TTTCTATGCG ATGGATGGTT GGAAGCTCAG CAACGAATTG
1081 CAATATGAGG TTCATCGATT GGGTGTAATT CG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Swiatecka-Urban, A., Myosin Vb is required for trafficking of the cystic fibrosis transmembrane conductance regulator in Rab11a-specific apical recycling endosomes in polarized human airway epithelial cells. J. Biol. Chem. 282 (32): 23725-23736 (2007). PMID 17462998. [link to RRC of NBRP]