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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse myosin Vb-tail

Expression vector of mouse myosin Vb tail region (Myo5b).

Catalog number RDB15286
Resource name pEF-FLAG-mouse myosin Vb-tail
Clone info. Expression vector of mouse myosin Vb tail region [Myo5b in NCBI], tagged with N-terminal FLAG, EF1 promoter. cDNA fragment 3853nt to 5619nt of NM_201600.2 is cloned.
Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : G to A substitution at 4419nt of NM_201600.2 (Arg, Synonym).
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo5b cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Myo5b (NCBI Gene 17919) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Swiatecka-Urban, A. et al., J. Biol. Chem. 282 (32): 23725-23736, 2007).
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15286 pEF-FLAG-mouse myosin Vb-tail DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Swiatecka-Urban, A., Myosin Vb is required for trafficking of the cystic fibrosis transmembrane conductance regulator in Rab11a-specific apical recycling endosomes in polarized human airway epithelial cells. J. Biol. Chem. 282 (32): 23725-23736 (2007). PMID 17462998.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15286_A7Gop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB15286_A7Goa.seq check
>D04773A4_A7Go_1_EF1a-C_F_D07_10_ABI24.ab1
    1 CCATTAATGA CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC
  121 GATTCCACCA TGGACTACAA GGACGATGAC GACAAGGGAT CCAGCTCCCC AGACAGCTAC
  181 AGCCTCCTCC TGAACCAGCT CAAGCTGGCC AATGAGGAGC TCGAGGTCCG CAAAGAGGAG
  241 GTGCTGATCC TCAGGACCCA GATCATGAAT GCCGACCAGC GCCGCCTGTC TGGCAAGAAC
  301 ATGGAGCCGA ACATCAATGC CAGAACAAGT TGGCCCAACA GTGAGAAGCA CGTGGACCAG
  361 GAAGACGCCA TTGAGGCCTA TCACGGGGTC TGCCAGACAA ACAGGTTGCT GGAGGCCCAG
  421 CTGCAGGCCC AGAGCCTGGA GCATGAGGAG GAGGTGGAAC ATCTCAAGGC CCAGGTGGAA
  481 GCCCTGAAAG AGGAGATGGA CAAACAGCAG CAGACCTTCT GCCAGACCCT GCTGCTCTCC
  541 CCAGAGGCCC AGGTAGAATT TGGTGTCCAG CAGGAGATAT CCCGGCTGAC CAATGAGAAC
  601 CTGGATTTTA AGGAATTGGT GGAAAAGCTG GAGAAGAATG AGAGGAAGCT GAAGAAGCAG
  661 CTGAAGATTT ACATGAAGAA GGTCCAGGAC TTAGAAGCTG CCCAGGCGTT GGCACAGAGT
  721 GACAGGAGGC ACCATGAACT CACAAGACAG GTCACAGTCC AACGAAAAGA GAAGGACTTC
  781 CAAGGCATGC TGGAGTACCA CAAAGAGGAC GAAGCCCTCC TCATCCGGAA CCTGGTGACA
  841 GACCTGAAGC CTCAGATGCT GTCGGGCACC GTGCCCTGTC TGCCTGCATA CATACTCTAT
  901 ATGTGCATCA CGCACGCGGA TTACACCAAC GATGACCTCA AAGGTGCACT CGTTGCTGAG
  961 CTCCACCATC AACGGCATTA AGAAAGTCCT CAAGAAGCAC AATGATGACT TTGAGATGAA
 1021 CGTCAATTCT GGCTATCCCA ACACTTGGCG GCTCGTCACT GTCTGAAGCA TTACAGTGAT
1081 TGAATGAGGG ATTTTCAATG GAACACAG
//
Primer: hCGSF_R
Sequence file: RDB15286_A7Gob.seq check
>D04773A4_A7Go_1_hGCSF_R_D08_11_ABI24.ab1
    1 CTGCTCTCAT CTCTCAGGGT CCCAGGTGGG GACCCTCACT CTAGAGCGGC CGCTATGGTC
   61 GACCTGCAGG CGGCCGCGAA TTCACTAGTG ATTTCAGACT TCATTGAGAA ACTCCAGGTT
  121 GAGACAGGCC GGGATGTGGA TCGAGTCCAT GGTCAGAGCA GATGGGTTAT ATGGAAACAG
  181 AACTGGGAAC ACGTGCTTGG AGTCCAGCAG GAGCTGCTGA GGGTCATTCC TCTCTTGTAG
  241 CTGAGCCTGG ATTGTTCGTA TGAAGGACAC TGTGACCCGT TCCTCAAATT CATTCAAGGG
  301 AGTGTAGAGG TTTAAAATTT TGACAATCTG CTGGGTGCTG AGGGAGGTGC ACAGAGAGCA
  361 GATGGCCTCA GCATCCTCGT GGGTTTTCTT CTTCAGCTGG AGGAGCTGGG CTGCCTGGAT
  421 GAGGGGCTCC ATGGTCTGAA CTGCTCCACT TTGGTGAAGG TTTTTGCCCC GAAGCCACTC
  481 TTCCAGTTGA CTTATGTTGT ACCTGAGTTG CATGCCTGTG CTCCAGGAGC AGGCGTCTTT
  541 CCGCAGGAGT AGGTTGTTAA GAGTCACAGC GTTGATCATG TAGAAGAGCT GTTTGAACAC
  601 CTGCAGGATA ATCTCGGGGT CCAGACCCTG GTCACACAGG ACTGTATGGA AAGAATTCAT
  661 CTGGCGGACG ATGGCCTCCA GGCAATACGA ATTCTCTCCA TCCACCATGC TGGAGGAGCG
  721 CTTCCGGTAA CCAGTTGGTC TCACACCAGA CAGCCCCTGG ATACTCTCAT TTTCCAACAT
  781 GGCAGAAACT ATCATAGGCT GCAGCAAGCC CTCGGCGATT TTAATGAGCT GCTGATAGAT
  841 CTGAATGGAA AGGTCGCTTA GCACCTGGCG GTATTCAGTG AGGTCAAAGT TCTTGAGACA
  901 GTGCTCATTC TGCTTCGCAG TGTTCTGTGT CATGAAACCC TCATCACCAC TGTATTGCTT
  961 CAGACAGTGA AGGAAGCGGC AGGTGTTGGG ATAGCCCAGA ATGACGTCAT CTCAAGTCAT
 1021 CCATTGTGCT TCTTGAGGAC TTTCTATGCG ATGGATGGTT GGAAGCTCAG CAACGAATTG
1081 CAATATGAGG TTCATCGATT GGGTGTAATT CG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Swiatecka-Urban, A., Myosin Vb is required for trafficking of the cystic fibrosis transmembrane conductance regulator in Rab11a-specific apical recycling endosomes in polarized human airway epithelial cells. J. Biol. Chem. 282 (32): 23725-23736 (2007). PMID 17462998.

2021.03.25

GNP_filter3_RDBDEP_html_210308.pl