Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCA-mIKKe-GFP (#RDB15222)

Expression vector of mouse I-kappaB kinase epsilon (IKK epsilon), tagged with GFP, CAG promoter.

Clone info. Expression vector of mouse I-kappaB kinase epsilon (IKK epsilon), tagged with GFP, CAG promoter.
Comment Expression was confirmed by the depositor with microscopy. pCA-Sal-EGFP, Ichii and Takeichi, Genes to Cell, 12: 827-839, 2007.
Vector backbone pCA-Sal-EGFP (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r
Gene/insert name mouse IKKepsilon cDNA
Depositor|Developer Hayashi, Shigeo |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Otani, T. et al., Dev. Cell, 20 (2): 219-232, 2011).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Otani, T. et al., Dev. Cell, 20 (2): 219-232, 2011)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15222 pCA-mIKKe-GFP Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCA-mIKKe-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15222).

Reference section:

Otani, T., Oshima, K., Onishi, S., Takeda, M., Shinmyozu, K., Yonemura, S., Hayashi, S., IKK epsilon regulates cell elongation through recycling endosome shuttling. Dev. Cell 20 (2): 219-232 (2011). PMID 21316589. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Otani, T., IKK epsilon regulates cell elongation through recycling endosome shuttling. Dev. Cell 20 (2): 219-232 (2011). PMID 21316589. [link to RRC of NBRP]