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pFN18A-HP1a-Shadow-Halo

Expression vector of Drosophila Heterochromatin protein 1a (HP1a), Chromo Shadoe domain

Catalog number RDB15212
Resource name pFN18A-HP1a-Shadow-Halo
Clone info. Expression vector of Drosophila truncated form of Heterochromatin protein 1a (HP1a) [Chromo Shadoe domain, 145 to 199 a.a], tagged with Halo, see Fig. 4A of Vo, N. et al. Nucleic Acids Res., 45 (3): 1233-1254, 2017.
Expression was confirmed by the depositor with western blotting.
Vector backbone pFN18A Flexi (plasmid)
Selectable markers Amp^r
Gene/insert name Drosophila dHP1 cDNA
Depositor Yamaguchi, Masamitsu |
 
Other clones in our bank Drosophila Su(var)205 (KEGG orthology K11587) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
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RDB15212 pFN18A-HP1a-Shadow-Halo DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15212_A8Ksp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Halo-C_F RDB15212_A8Ksa.seq
>D04544B2_A8Ks_2_Halo-C_F_A06_03_ABI24.ab1
    1 GGGCCGGACT TGCTGCAGAA GACACCCGGA CCTGATCGGC AGCGAGATCG CGCGCTGGCT
   61 GTCGACGCTC GAGATTTCCG GCGAGCCAAC CACTGAGGAT CTGTACTTTC AGAGCGATAA
  121 CGCGATCGCC CGCGGCCTGG AGGCCGAAAA GATCTTGGGT GCCTCCGACA ATAATGGCCG
  181 CCTGACATTC CTCATTCAGT TCAAAGGCGT GGACCAAGCA GAAATGGTGC CCTCCTCAGT
  241 GGCCAATGAA AAAATCCCAC GAATGGTAAT CCACTTCTAC GAAGAGCGCC TATCCGTTTA
  301 AACGAATTCG GGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTG
  361 ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT
  421 AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG
  481 GAACTATATC CGGTTCGCTT GCTGTCCATA AAACCGCCCA GTCTAGCTAT CGCCATGTAA
  541 GCCCACTGCA AGCTACCTGC TTTCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC
  601 AGTAGCTGAC ATTCATCCGG GGTCAGCACC GTTTCTGCGG ACTGGCTTTC TACGTAATGG
  661 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT
  721 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC
  781 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT
  841 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG
  901 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA
  961 AGATCCTTGA GAGTTTTCGC CCCGAAAGAA CGTTTTCCCA ATGATGAGCA CTTTCAAAGT
 1021 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCCGG GCAAGAGCAA CTCGGGTCGG
 1081 CGGCATACAC TATTCTCAGA ATGGACCTGT TGAGTACTTC ACCAGGTCAC AGAAAAAGGC
1141 ATCTTTAACC GGAAAT
//
Primer: rrnBT1ter_F RDB15212_A8Ksb.seq
>D04544B2_A8Ks_2_rrnBT1ter_F_F01_06_ABI08.ab1
    1 TGGGGGGGGG TTTTGAACGT TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC
   61 GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC
  121 GTTTCTACAA ACTCTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGG
  181 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGTA TTTTAACTTT ACTAAGGAGA
  241 ATTCATCATG GCAGAAATCG GTACTGGCTT TCCATTCGAC CCCCATTATG TGGAAGTCCT
  301 GGGCGAGCGC ATGCACTACG TCGATGTTGG TCCGCGCGAT GGCACCCCTG TGCTGTTCCT
  361 GCACGGTAAC CCGACCTCCT CCTACGTGTG GCGCAACATC ATCCCGCATG TTGCACCGAC
  421 CCATCGCTGC ATTGCTCCAG ACCTGATCGG TATGGGCAAA TCCGACAAAC CAGACCTGGG
  481 TTATTTCTTC GACGACCACG TCCGCTTCAT GGATGCCTTC ATCGAAGCCC TGGGTCTGGA
  541 AGAGGTCGTC CTGGTCATTC ACGACTGGGG CTCCGCTCTG GGTTTCCACT GGGCCAAGCG
  601 CAATCCAGAG CGCGTCAAAG GTATTGCATT TATGGAGTTC ATCCGCCCTA TCCCGACCTG
  661 GGACGAATGG CCAGAATTTG CCCGCGAGAC CTTCCAGGCC TTCCGCACCA CCGACGTCGG
  721 CCGCAAGCTG ATCATCGATC AGAACGTTTT TATCGAGGGT ACGCTGCCGA TGGGTGTCGT
  781 CCGCCCGCTG ACTGAAGTCG AGATGGACCA TTACCGCGAG CCGTTCCTGA ATCCTGTTGA
  841 CCGCGAGCCA CTGTGGCGCT TCCCAAACGA GCTGCCAATC GCCGGTGAGC CAGCGAACAT
  901 CGTCGCGCTG GTCGAAGAAT ACATGGGACT GGCTGCACCA GTCCCCTGTC CCGAAGCTGC
  961 TGTTCTGGGG GCACCCCAGG CGTTCTGATC CCACCGGGCC GAAGCCCGCT CGGCTTGACA
 1021 AAAGCCTGCC CTAACTGCAA GCTGTGGACA TCGGCCGGTC TGAATCTGCT GCAAGGAAGA
1081 ACCCCTGACC TTGATTCGGC AGCCGAAGAA TCGCGCGCTT GGCTTGTTCT CGTCG
//
Primer: pTrcHis-R RDB15212_A8Ksc.seq
>D04544B2_A8Ks_2_pTrcHiS-R_B06_06_ABI24.ab1
    1 CGGGCCGTAA GAACTCGATC CTCTACGCCG GACGCATCGT GGCCGGAGTA CTGCGGTTTA
   61 TCTCGATGGC TACGAGGGCA GACAGTAAGT GGATTTACCA TAATCCCTTA ATTGTACGCA
  121 CCGCTAAAAC GCGTTCAGCG CGATCACGGC AGCAGACAGG TAAAAATGGC AACAAACCAC
  181 CCGAAAAACT GCCGCAATCG CGCCTGATAA ATTTTAACCG TATGAATACC TATGCAACCA
  241 GAGGGTACAG GCCACATTAC CCCCACTTAA TCCACTGAAG CTGCCATTTT TCATGGTTTC
  301 ACCATCCCAG CGAAGGGCCA TCCAGCGTGC GTTCCTGTAT TTCCGGACTA TAAAAGGATC
  361 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC
  421 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAAATCC TTTTTTTCTG
  481 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG
  541 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
  601 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
  661 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
  721 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
  781 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
  841 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
  901 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGGAAACGC
  961 CTGGTATCTT TATAGTCCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
 1021 GATGCTCGTC AGGGGGGCGG AGCCCTATGG AAAAACGCCA GCACGCGGGC CTTTTTTAAC
 1081 GGATCTGGCC TTTTGCTGGG CCGTATGGCT TCACATGGAT CGTTCCTGCG TATCCCCTGG
1141 AATCTGTGAT ACGCTATTAC GGCCTTTTTT TGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

2019.06.11

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