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pET16b-fullHP1a-His (#RDB15203)

Expression vector of Drosophila Heterochromatin protein 1a (HP1a)

Clone info. Expression vector of Drosophila Heterochromatin protein 1a (HP1a) [full length, 1 to 206 a.a], tagged with His.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pET-16b (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Drosophila dHP1 cDNA
Depositor|Developer Yamaguchi, Masamitsu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15203 pET16b-fullHP1a-His DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET16b-fullHP1a-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15203).

Reference section:

Vo, N., Anh Suong, D.N., Yoshino, N., Yoshida, H., Cotterill, S., Yamaguchi, M., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15203_A8Ksp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: phRLR1 ()
Sequence file: RDB15203_A8Ksa.seq check
>D04544A2_A8Ks_3_phRLR1_F01_16_ABI24.ab1
    1 ATGTCATGAG CGGATACATA TTTGATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
   61 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC
  121 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTTCAA GAATTCTTAA TCTTCATTAT
  181 CAGAGTACCA GGATAGGCGC TCTTCGTAGA AGTGGATTAC CATTCGTGGG ATTTTTTCAT
  241 TGGCCACTGA GGAGGGCACC ATTTCTGCTT GGTCCACGCC TTTGAACTGA ATGAGGAATG
  301 TCAGGCGGCC ATTATTGTCG GAGGCACCCA AGATCTTTTC GGCCTCCAGG CCGCGATCGA
  361 ATCCGGTAGA TCCTGAAACG GGAATGGTGT CCTGCTCCGC ATCTGTGGTA CGTTTTGATT
  421 TATTGCCAGA GGGCGCTGTT GGTTCTTCGG ACTTTCGCTT GCTTGCTGTG GACGTCGAAC
  481 TGCTGGCGCG TCCTTGAGTT TCCTTGGCCT TGGCGCTGCT GCTGGGTCGA TCCTTCTTGG
  541 AGGCGGCTGA CTTCTCCTCA TCCTTGCGGC TCGCCTCGTA CTGCTGGATA AGATCCTGGC
  601 AGTCGAGATT GTTCTCCGGC TCCCACGTGT TCTCAGTTTC GGGATAGCCC TTCCATTTCA
  661 GATAGTACTC CACCTTTCCC TTGCGCACCC GCCTGTCGAT GATCTTTTCC ACGGCGTACT
  721 CCTCCTCCTC CTCTTCGGCA TCGGAAACCT TTGCCGAGCT CTCAGGGTTG TCGATTTTCT
  781 TGCCCATCTC GAGCATATGA CGACCTTCGA TATGGCCGCT GCTGTGATGA TGATGATGAT
  841 GATGATGATG ATGGCCCATG GTATATCTCC TTCTTAAAGT TAAACAAAAT TATTTCTAGA
  901 GGGGAATTGT TATCCGCTCA CAATTCCCCT ATAGTGAGTC GTATTAATTT CGCGGGATCG
  961 AGATCTCGAT CCTCTACGCC GGACGCATCG TGGCCCGGCA TCACCGGCGC CACAGGTGCG
 1021 GGTTGCTGGG CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT CGCCACTTCG
 1081 GCTCATGAGC GCTTTGTTTC GGCGTGGGTA TGGTGGCAGG CCCCGGTGGA CGGGGGACTG
1141 TTTGGGGCCG GCCAATTCCT CTCCTTTCA
//
Primer: lacIpro_F ()
Sequence file: RDB15203_A8Ksb.seq check
>D04544A2_A8Ks_3_lacIpro_F_G01_19_ABI24.ab1
    1 TTTCGGCTGA TGCGCCGGAG AGAGTCATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA
   61 TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG
  121 GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT
  181 TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT
  241 GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC
  301 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC
  361 TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT
  421 CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA
  481 TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT
  541 ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG
  601 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT
  661 CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT
  721 CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC
  781 GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG
  841 GATATCTCGG TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA
  901 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA
  961 CTCTCTCAGG CCCAGGCGGT GAAGGGCAAT CAGCTGTTTG CCCGTCTCAC TGGTGAAAAG
 1021 AAAAACCACC CTGGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG GCGATCATTA
 1081 ATTGCAGCTG GGCACGACAG TTCCGGAACT TGCAAGCCGG GCAGTGAGCG CAACGCCAAC
1141 TTAATGTTAA AGGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. [link to RRC of NBRP]