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pCMV-Mreg delta C/MST-mStr-Rab27A-QL/CA

Expression vector of mouse Rab27A mutant (Q78L) lacking C-terminal geranylgeranylation sites (C219A, C221A).

Catalog number RDB15185
Resource name pCMV-Mreg delta C/MST-mStr-Rab27A-QL/CA
Clone info. Expression vector of mouse Rab27A mutant (Q78L) lacking C-terminal geranylgeranylation sites (C219A, C221A), tagged with N-terminal MST and C-terminal mStr, CMV promoter, see Fig2A of Ishida, M. et al., J. Biol. Chem., 289 (16): 11059-11067, 2014.
Expression was confirmed by the depositor with fluorescence microscope (negative control).
Vector backbone pmStr-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab27a cDNA mouse Mreg cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab27a (NCBI Gene 11891) | mouse Mreg (NCBI Gene 381269) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Cell Sci. 125; 125 (Pt6): 1508-1518, 2012); (Pigment Cell Melanoma Res. 23 (3): 365-374, 2010); (Genes Cells 11 (9): 1023-1037, 2006); (J. Biol. Chem. 289 (16): 11059-11067, 2014)
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15185 pCMV-Mreg delta C/MST-mStr-Rab27A-QL/CA Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB15185pka01.seq

Original reference

original Ishida, M., The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targeting tag. J. Biol. Chem. 289 (16): 11059-11067 (2014). PMID 24584932.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Ishida, M., The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targeting tag. J. Biol. Chem. 289 (16): 11059-11067 (2014). PMID 24584932.
reference Ohbayashi, N., Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes. J. Cell Sci. 125 (Pt 6): 1508-1518 (2012). PMID 22275436.
reference Ohbayashi, N., Functional characterization of two RAB27A missense mutations found in Griscelli syndrome type 2. Pigment. Cell Melanoma Res. 23 (3): 365-374 (2010). PMID 20370853.
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

2021.05.03

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