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pCMV-Mreg delta C/MST-mStr-Rab27A-QL/CA (#RDB15185)

Expression vector of mouse Rab27A mutant (Q78L) lacking C-terminal geranylgeranylation sites (C219A, C221A).

Clone info. Expression vector of mouse Rab27A mutant (Q78L) lacking C-terminal geranylgeranylation sites (C219A, C221A), tagged with N-terminal MST and C-terminal mStr, CMV promoter, see Fig2A of Ishida, M. et al., J. Biol. Chem., 289 (16): 11059-11067, 2014.
Comment Expression was confirmed by the depositor with fluorescence microscope (negative control).
Vector backbone pmStr-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rab27a cDNA mouse Mreg cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (partial) RDB15185pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Cell Sci. 125; 125 (Pt6): 1508-1518, 2012); (Pigment Cell Melanoma Res. 23 (3): 365-374, 2010); (Genes Cells 11 (9): 1023-1037, 2006); (J. Biol. Chem. 289 (16): 11059-11067, 2014)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Cell Sci. 125; 125 (Pt6): 1508-1518, 2012); (Pigment Cell Melanoma Res. 23 (3): 365-374, 2010); (Genes Cells 11 (9): 1023-1037, 2006); (J. Biol. Chem. 289 (16): 11059-11067, 2014)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15185 pCMV-Mreg delta C/MST-mStr-Rab27A-QL/CA Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCMV-Mreg delta C/MST-mStr-Rab27A-QL/CA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15185).

Reference section:

Ishida, M., Arai, S.P., Ohbayashi, N., Fukuda, M., The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targeting tag. J. Biol. Chem. 289 (16): 11059-11067 (2014). PMID 24584932. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ishida, M., The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targeting tag. J. Biol. Chem. 289 (16): 11059-11067 (2014). PMID 24584932. [link to RRC of NBRP]
reference Ohbayashi, N., Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes. J. Cell Sci. 125 (Pt 6): 1508-1518 (2012). PMID 22275436. [link to RRC of NBRP]
reference Ohbayashi, N., Functional characterization of two RAB27A missense mutations found in Griscelli syndrome type 2. Pigment. Cell Melanoma Res. 23 (3): 365-374 (2010). PMID 20370853. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]