MST tag, a novel melanosome-targeting tag.
Clone info. | MST tag, a novel melanosome-targeting tag. MST tag expression vector, mouse melanoregulin (Mreg) lacking a stop codon and of an N-terminal portion of Mreg (1 to 139 AA), fused with C-terminal mStr, CMV promoter, see Fig2A of Ishida, M. et al., J. Biol. Chem., 289 (16): 11059-11067, 2014. |
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Comment | Expression was confirmed by the depositor with fluorescence microscope. |
Vector backbone | pmStr-N1 (Plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | mouse Mreg cDNA |
Depositor|Developer | Fukuda, Mitsunori | |
Sequence (partial) | RDB15184pka01.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Cell Sci. 125; 125 (Pt6): 1508-1518, 2012); (J. Biol. Chem. 289 (16): 11059-11067, 2014) |
Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Cell Sci. 125; 125 (Pt6): 1508-1518, 2012); (J. Biol. Chem. 289 (16): 11059-11067, 2014)。 |
備考 | Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15184 | pCMV-Mreg delta C/MST-mStr | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pCMV-Mreg delta C/MST-mStr was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15184). |
Reference section:
Ishida, M., Arai, S.P., Ohbayashi, N., Fukuda, M., The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targeting tag. J. Biol. Chem. 289 (16): 11059-11067 (2014). PMID 24584932. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Ishida, M., The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targeting tag. J. Biol. Chem. 289 (16): 11059-11067 (2014). PMID 24584932. [link to RRC of NBRP] |
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reference | Ohbayashi, N., Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes. J. Cell Sci. 125 (Pt 6): 1508-1518 (2012). PMID 22275436. [link to RRC of NBRP] |
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