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pCMV-Mreg delta C/MST-mStr

MST tag, a novel melanosome-targeting tag.

Catalog number RDB15184
Resource name pCMV-Mreg delta C/MST-mStr
Clone info. MST tag, a novel melanosome-targeting tag. MST tag expression vector, mouse melanoregulin (Mreg) lacking a stop codon and of an N-terminal portion of Mreg (1 to 139 AA), fused with C-terminal mStr, CMV promoter, see Fig2A of Ishida, M. et al., J. Biol. Chem., 289 (16): 11059-11067, 2014.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pmStr-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Mreg cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Mreg (NCBI Gene 381269) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Cell Sci. 125; 125 (Pt6): 1508-1518, 2012); (J. Biol. Chem. 289 (16): 11059-11067, 2014)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15184 pCMV-Mreg delta C/MST-mStr Under QC test. Please contact us. DNA solution

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Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB15184pka01.seq

Original reference

original Ishida, M., The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targeting tag. J. Biol. Chem. 289 (16): 11059-11067 (2014). PMID 24584932.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Visualization of Organelles (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Ishida, M., The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targeting tag. J. Biol. Chem. 289 (16): 11059-11067 (2014). PMID 24584932.
reference Ohbayashi, N., Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes. J. Cell Sci. 125 (Pt 6): 1508-1518 (2012). PMID 22275436.

2021.05.03

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