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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-mouse T7-Munc13-4

Expression vector of mouse Munc13-4 [Unc13d in NCBI].

Catalog number RDB15181
Resource name pEF-mouse T7-Munc13-4
Clone info. Expression vector of mouse Munc13-4 [Unc13d in NCBI], tagged with T7, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-T7 (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Unc13d cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Unc13d

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Pigment Cell Melanoma Res. 23 (3): 365-374, 2010)
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Pigment Cell Melanoma Res. 23 (3): 365-374, 2010)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15181 pEF-mouse T7-Munc13-4 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (partial) RDB15181pka01.seq provided by the depositor

          Original reference

          original Ohbayashi, N., Functional characterization of two RAB27A missense mutations found in Griscelli syndrome type 2. Pigment. Cell Melanoma Res. 23 (3): 365-374 (2010). PMID 20370853.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15181_A7Fmp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB15181_A7Fma.seq check
          >D04709B7_1_A7Fm_EF1a-C_F_D04_10_ABI24.ab1
              1 CAGGGGATGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
             61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
            121 CGATTCCACC ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGCGGAT CTATGGCGAC
            181 ACACCTTTCC CACCCCCAGC GGCGTCCACT CTTGCGCCAG GCCATCAAGA TAAGGCGCCG
            241 CAGGGTCAGA GACCTGCAGG ATCCACCACC CCAAGCCACC CAGGAGGTCC AGGTTCAGTC
            301 CCACCATTTC TCCCCAGAGG AGCGGGACCT GCTGTATGAG GAAGCCCTCT ATACTGTCCT
            361 GCACCGCCTG GGTCAGCCAG AGCCCAACCA TGTGAAGGAG GCCTCGGAAC TGCTGAGCTA
            421 TCTGCAGGAG GCTTTCCAGG TGCAGCCTGA GGAGCACCAG CAGATGCTGC GGCGCGTCAG
            481 GGAGCTCGAG AAGCCAGTAT TTTGCCTGAA GGCCACAGTG AAACAGGCCA AGGGTATTCT
            541 GGGAAAAGAT GTCAGCGGGT TCAGCGACCC CTACTGCCTG CTGGGCATCG AGCAGAAGGT
            601 TGGTGTAGCA GAGGGCAGCC CTGTGTCCCG ACGTCGGCAG AAGGCAGTGG TGAAGCACAC
            661 CATCCCGGAA GAGGAGACCC ATCGCACCCA AGTCAAGAGC CAGACACTTA ATCCCGTCTG
            721 GGACGAGACC TTTATCTTGG AGTTTGAGGA CATAGCCAAT GCAAGCTTTC ACCTGGACAT
            781 GTGGGACCTG GACACTGTGG AGTCTGTCAG GCAGAAGCTC GGGGAGCTCA CGGACCTGCA
            841 CGGGCTCCGG AGGATCTTTA AAGAAGCTCG GAAGGATAAA GGCCAGGACG ACTTTCTGGG
            901 GAATGTGGTT CTGAGGTTGC AGGACCTGCG CTGCCGAGAG GACCAGTGGT TCCCGCTAGA
            961 GCCCTGCACA GAGACCTACC CAGACCGCGG GCCAGTGCCA CCTTCAGTTT CCAGTTCATT
           1021 CACAAGAGGA GAGCCACGGG CGGGCCCAGC CGCTCTCAGC CCAGCTACAC TGTTACACTT
           1081 TCACTACTGC AGCAGCTGAT GTCCATGAGT TCACACAGCA CCAAGCTGGC AGGTAACCTC
          1141 CTGGGGAACC GCCAATTCAA
          //
          Primer: hGCSF-R
          Sequence file: RDB15181_A7Fmb.seq check
          >D04709B7_1_A7Fm_hGCSF-R_E04_13_ABI24.ab1
              1 CTGGGGGAAT GAAACTCTCA GGGTCCCAGG TGGGGACCCT CACTCTAGAG CGGCCGCTAT
             61 GGTCGACCTG CAGGCGGCCG CGAATTCACT AGTGATTTCA CGGGGCATGT TGGGAGGCCT
            121 GCTTGGCTCT CTGCCTCCTC AGCTTTACAA AGGCCTGGGC CTCGCGATCC CCCTTCCGGC
            181 TCTCCAACAG CCGCAGAATT GGGTCCCCGT TGGGGGCAGG GTATGTGAGA GGCAGGCGCA
            241 TTTGAGGTGC TTCGCCCGGC TCTGCACAGT CCGTCAGTCC AGGTACCCTG CAGAGCGGTA
            301 AGAAGGCCTC TCCCTCCAGG TCGTCTGCTC CCAGTCTGTC GTGGTCCAGC ACAGTGAGCA
            361 GGAGGCATGC CCAGGCTTTT TGGCAAGGCT CAGCAGGCAC CAGGAATTCA AAGGTCTCAT
            421 CAAAGAGTGG GTGAAGTTCC TTCTTGTGCT TCTGGGTCTC CCGGGGGGCC ACTTCAGGGA
            481 ATTCATGTCT GGGTTCCAGT GTCAACTGAA CAAAGGGGTC ACTGGAACCA TTGGAGTCCA
            541 GGGGCAGCAG GCTAGAAGCA CTGAGCAGTT CCACGCGAAG CCTCTGCTCA GAGGCGCGGT
            601 AGGAGACCTT GACGGTGACT GCGCCCAGCC TCTCAGAAGT GGTTTCGGCC TGCTGCTGGA
            661 TTCGGCTGCA GAAGTACTTC TGGATAAGCT CCCGGCTGGA GGCCGCCTGC AGCTCCAGGT
            721 CGTTCTGCAG AGCCTGGAAG GTGTCTGTGT GCAGGGCCTC TGGTGGCAGA CCACAGCCCT
            781 CAGCGTGGAA GCAGACCTCC AGGTTCTGAA GGGCGACCTT CAGCCTGCCA GAAGCCAGGG
            841 ACGAGCTACG CTGGGAAGAA GCCACCTCCA CCAGCACAGT AAGCGTGTGG GTCCACAACA
            901 GAGTCAGAAG GCTGCTGAAG TTCTCCTGGA CCAGGTTGGT GTTCATGTAG CAAAGCTTCA
            961 CCTCCAAGGA ATTTCATCAC GGGCAGAATG GCATCCCTCG GGGCAGAACA GACTCCCTTG
           1021 ACGCCCAATG AGTTTTCTGG GATGTGTGTG GGCAATGCCC ACCTCCCAAC TGCTCTGCCA
           1081 AGGGTACGGG ACACCAGTAC GGATTCTCAA TTGCCAGGCC CCGGCCCAAG GGCGGCCCTT
          1141 GCCAGCTGAA GCATGTACGT GATCTGCAAA GCCTGCCCCA TTCCAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ohbayashi, N., Functional characterization of two RAB27A missense mutations found in Griscelli syndrome type 2. Pigment. Cell Melanoma Res. 23 (3): 365-374 (2010). PMID 20370853.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl