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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-mouse T7-Munc13-4 (#RDB15181)

Expression vector of mouse Munc13-4 [Unc13d in NCBI].

Clone info. Expression vector of mouse Munc13-4 [Unc13d in NCBI], tagged with T7, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-T7 (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Unc13d cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (partial) RDB15181pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Pigment Cell Melanoma Res. 23 (3): 365-374, 2010)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Pigment Cell Melanoma Res. 23 (3): 365-374, 2010)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15181 pEF-mouse T7-Munc13-4 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-mouse T7-Munc13-4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15181).

Reference section:

Ohbayashi, N., Mamishi, S., Ishibashi, K., Maruta, Y., Pourakbari, B., Tamizifar, B., Mohammadpour, M., Fukuda, M., Parvaneh, N., Functional characterization of two RAB27A missense mutations found in Griscelli syndrome type 2. Pigment. Cell Melanoma Res. 23 (3): 365-374 (2010). PMID 20370853. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15181_A7Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Sequence file: RDB15181_A7Fma.seq check
>D04709B7_1_A7Fm_EF1a-C_F_D04_10_ABI24.ab1
    1 CAGGGGATGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGCGGAT CTATGGCGAC
  181 ACACCTTTCC CACCCCCAGC GGCGTCCACT CTTGCGCCAG GCCATCAAGA TAAGGCGCCG
  241 CAGGGTCAGA GACCTGCAGG ATCCACCACC CCAAGCCACC CAGGAGGTCC AGGTTCAGTC
  301 CCACCATTTC TCCCCAGAGG AGCGGGACCT GCTGTATGAG GAAGCCCTCT ATACTGTCCT
  361 GCACCGCCTG GGTCAGCCAG AGCCCAACCA TGTGAAGGAG GCCTCGGAAC TGCTGAGCTA
  421 TCTGCAGGAG GCTTTCCAGG TGCAGCCTGA GGAGCACCAG CAGATGCTGC GGCGCGTCAG
  481 GGAGCTCGAG AAGCCAGTAT TTTGCCTGAA GGCCACAGTG AAACAGGCCA AGGGTATTCT
  541 GGGAAAAGAT GTCAGCGGGT TCAGCGACCC CTACTGCCTG CTGGGCATCG AGCAGAAGGT
  601 TGGTGTAGCA GAGGGCAGCC CTGTGTCCCG ACGTCGGCAG AAGGCAGTGG TGAAGCACAC
  661 CATCCCGGAA GAGGAGACCC ATCGCACCCA AGTCAAGAGC CAGACACTTA ATCCCGTCTG
  721 GGACGAGACC TTTATCTTGG AGTTTGAGGA CATAGCCAAT GCAAGCTTTC ACCTGGACAT
  781 GTGGGACCTG GACACTGTGG AGTCTGTCAG GCAGAAGCTC GGGGAGCTCA CGGACCTGCA
  841 CGGGCTCCGG AGGATCTTTA AAGAAGCTCG GAAGGATAAA GGCCAGGACG ACTTTCTGGG
  901 GAATGTGGTT CTGAGGTTGC AGGACCTGCG CTGCCGAGAG GACCAGTGGT TCCCGCTAGA
  961 GCCCTGCACA GAGACCTACC CAGACCGCGG GCCAGTGCCA CCTTCAGTTT CCAGTTCATT
 1021 CACAAGAGGA GAGCCACGGG CGGGCCCAGC CGCTCTCAGC CCAGCTACAC TGTTACACTT
 1081 TCACTACTGC AGCAGCTGAT GTCCATGAGT TCACACAGCA CCAAGCTGGC AGGTAACCTC
1141 CTGGGGAACC GCCAATTCAA
//
Primer: hGCSF-R ()
Sequence file: RDB15181_A7Fmb.seq check
>D04709B7_1_A7Fm_hGCSF-R_E04_13_ABI24.ab1
    1 CTGGGGGAAT GAAACTCTCA GGGTCCCAGG TGGGGACCCT CACTCTAGAG CGGCCGCTAT
   61 GGTCGACCTG CAGGCGGCCG CGAATTCACT AGTGATTTCA CGGGGCATGT TGGGAGGCCT
  121 GCTTGGCTCT CTGCCTCCTC AGCTTTACAA AGGCCTGGGC CTCGCGATCC CCCTTCCGGC
  181 TCTCCAACAG CCGCAGAATT GGGTCCCCGT TGGGGGCAGG GTATGTGAGA GGCAGGCGCA
  241 TTTGAGGTGC TTCGCCCGGC TCTGCACAGT CCGTCAGTCC AGGTACCCTG CAGAGCGGTA
  301 AGAAGGCCTC TCCCTCCAGG TCGTCTGCTC CCAGTCTGTC GTGGTCCAGC ACAGTGAGCA
  361 GGAGGCATGC CCAGGCTTTT TGGCAAGGCT CAGCAGGCAC CAGGAATTCA AAGGTCTCAT
  421 CAAAGAGTGG GTGAAGTTCC TTCTTGTGCT TCTGGGTCTC CCGGGGGGCC ACTTCAGGGA
  481 ATTCATGTCT GGGTTCCAGT GTCAACTGAA CAAAGGGGTC ACTGGAACCA TTGGAGTCCA
  541 GGGGCAGCAG GCTAGAAGCA CTGAGCAGTT CCACGCGAAG CCTCTGCTCA GAGGCGCGGT
  601 AGGAGACCTT GACGGTGACT GCGCCCAGCC TCTCAGAAGT GGTTTCGGCC TGCTGCTGGA
  661 TTCGGCTGCA GAAGTACTTC TGGATAAGCT CCCGGCTGGA GGCCGCCTGC AGCTCCAGGT
  721 CGTTCTGCAG AGCCTGGAAG GTGTCTGTGT GCAGGGCCTC TGGTGGCAGA CCACAGCCCT
  781 CAGCGTGGAA GCAGACCTCC AGGTTCTGAA GGGCGACCTT CAGCCTGCCA GAAGCCAGGG
  841 ACGAGCTACG CTGGGAAGAA GCCACCTCCA CCAGCACAGT AAGCGTGTGG GTCCACAACA
  901 GAGTCAGAAG GCTGCTGAAG TTCTCCTGGA CCAGGTTGGT GTTCATGTAG CAAAGCTTCA
  961 CCTCCAAGGA ATTTCATCAC GGGCAGAATG GCATCCCTCG GGGCAGAACA GACTCCCTTG
 1021 ACGCCCAATG AGTTTTCTGG GATGTGTGTG GGCAATGCCC ACCTCCCAAC TGCTCTGCCA
 1081 AGGGTACGGG ACACCAGTAC GGATTCTCAA TTGCCAGGCC CCGGCCCAAG GGCGGCCCTT
1141 GCCAGCTGAA GCATGTACGT GATCTGCAAA GCCTGCCCCA TTCCAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohbayashi, N., Functional characterization of two RAB27A missense mutations found in Griscelli syndrome type 2. Pigment. Cell Melanoma Res. 23 (3): 365-374 (2010). PMID 20370853. [link to RRC of NBRP]