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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-mouse FLAG-Munc13-1 (#RDB15180)

Expression vector of mouse Munc13-1 [Unc13b in NCBI].

Clone info. Expression vector of mouse Munc13-1 [Unc13b in NCBI], tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Unc13b cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Genes Cells 9(9): 831-842, 2004)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Genes Cells 9(9): 831-842, 2004)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15180 pEF-mouse FLAG-Munc13-1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-mouse FLAG-Munc13-1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15180).

Reference section:

Fukuda, M., Alternative splicing in the first alpha-helical region of the Rab-binding domain of Rim regulates Rab3A binding activity: is Rim a Rab3 effector protein during evolution? Genes Cells 9 (9): 831-842 (2004). PMID 15330860. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15180_A7Fmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB15180_A7Fma.seq check
>D04709B6_2_A7Fm_EF1a-C_F_B04_04_ABI24.ab1
    1 CCTGGGATGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCATGTCGC TGCTCTGTGT
  181 GCGTGTTAAA AGAGCCAAAT TCCAGGGTTC ACCAGATAAA TTTAACACAT ATGTGACCCT
  241 GAAAGTCCAG AACGTGAAGA GCACAACTGT AGCAGTTCGT GGTGATCAGC CTTCCTGGGA
  301 GCAGGATTTC ATGTTTGAGA TCAGTCGTCT GGACCTGGGC CTAAGTGTGG AGGTGTGGAA
  361 CAAGGGACTG ATATGGGACA CAATGGTGGG CACCGTGTGG ATTGCACTGA AGACAATCCG
  421 TCAGTCGGAT GAGGAAGGGC CCGGGGAGTG GTCTACACTG GAGGCAGAGA CGTTAATGAA
  481 AGATGATGAG ATCTGTGGAA CTAAAAACCC AACTCCTCAT AAAATCTTGC TTGATACAAG
  541 ATTTGAGCTG CCTTTTGACA TCCCAGAGGA GGAAGCCAGA TATTGGACCT ACAAATTGGA
  601 GCAAATCAAT GCCTTGGCGG ATGATAATGA GTATTCTAGT CAAGAAGAAA GCCAGAGGAA
  661 GCCATTACCC ACTGCTGCCG CCCAGTGTTC CTTTGAAGAC CCTGATAGTG CCGTTGATGA
  721 CCGAGACAGT GACTACCGCA GTGAGACCAG CAATAGCGCC CCACCTCCTT ACCACACGAC
  781 TACCCAGCCC AACGCTTCTG TGCACCAGTT CCCTGTTCCT GTGCGGCTGC CACAGCAGCT
  841 CTTTCTTCAA GGCAGTTCCC ATGACTCTTG CAATGACTCT ATGCAGAGTT ACGACCTTGA
  901 TTATCCAGAG CGTCGGGCCC TCAGCCCTAC CAGCAGTAGT AGGTATGGCT CCTCCTGTAA
  961 TGTGAGTCAA GGAAGCTCTC TGCTGAGTGA ACTGGACCAG TATCATGAGC AAGATGATGA
 1021 TGGGTCGGGG AGAGGGACTC CATTTCATTC TAGTCATAGC TATGGGCAGC CTCCTCCAAG
 1081 ATGACCAGGC TGGGATTTGG AGACAAGGAG AAGCCCTTGT AGTGACATGT GAATTCAGTA
1141 CAGAGCAATC GTTGCATTCC GAAAAGGCCA GGAT
//
Primer: hGCSF_R
Sequence file: RDB15180_A7Fmb.seq check
>D04709B6_2_A7Fm_hGCSF-R_C04_07_ABI24.ab1
    1 TGGGGAAATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCTATGG
   61 TCGACCTGCA GGCGGCCGCG AATTCACTAG TGATTTCAGC TCCCTTCCTC TGTGGATCGA
  121 GACTCTGATT TGAGCTTCAC GAACTCTCGG GCTACCTCGT CATTGCTCCT CTGAGACAGA
  181 ATCCGGAGAA TGGTCATGCC TGTCTCATCC ATATGGATCT TCCGGCCCAG TGGGCACCAG
  241 CAGGCACAGC TGCCCTTAGC TGCCACATCC CTCAGGGGCA TCACTGCCAG TCCTATCACA
  301 CGATCCTCCC GGGCAAAGCA GTAATCCTTC ACACAGATCT GCAATTCATA GGCCTCTGGA
  361 CCCTCTTCAT TTCCCAGGAG GAAGTGAAAC GTCTCGTTGT ACTTGGGAGT CCAGTTGTTA
  421 CTTTTAGACT TGGTTGTGAA CTTCCTCTTC TTATCACTTT GGTGTGGGCC AACCATGGTC
  481 ACTTCCACAA AAGGCCGGAA CATACCCGCT GTCTGCCACT TGAGGTCATT GGCTGCTACC
  541 ACTTTCACTG TGACCTTATG CTCCCCAGTG CCAGGATGTG TAAATAAGTC CACCTGAATA
  601 GAGACTTCTC CCACGGGGTC ATCCACACCA GCCCCCTGGG CAGTCTGTGA GCGCACAAAT
  661 GTCTTGATGA GAGTGTCTGT AGTCTGTGTA TACAGAGAGA GGGCATAACG CAGAGACTGC
  721 AGGTCTGGGC TCTTTTCCAG GAAGGTTTTC TTCAGACCAT TGCCTCCTGC ATGGAAGTAT
  781 TGCTTGATGG TGTCCAAGGC CAAGTCGAGG ACAGCACACT GCTTTGGAGT GAGATTCCGT
  841 GTTTCCTCCC GTACCATGTG GTCCTTGAGT TTGGAAAGCT GGCTCAGCTC CTTGGCAGCA
  901 GTTAAGATCA GCTGGGTGCC CGTCTGGTCA GTGAGTGGGG GCAGGACAAT AACCCGCTCC
  961 ATGGTGTTCA TCACCACCCG CCAGAGCTCC TTCAGGACCC GCTTCAGAAC TGTCTTCTCA
 1021 CACACAGTGG CAAACAGCGT GAGGTTGCCC ATCCAGGAAG TCCATGAGAG GTCGCAGCAC
 1081 ACTATTCTGC ATCCTGAGCC ACCGAAGTCC CTTGGCCGTA AGGAATGCAT CCTGTGCTCC
1141 GAACTTGGAC CCAAGGAATG TTCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Alternative splicing in the first alpha-helical region of the Rab-binding domain of Rim regulates Rab3A binding activity: is Rim a Rab3 effector protein during evolution? Genes Cells 9 (9): 831-842 (2004). PMID 15330860. [link to RRC of NBRP]